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    Polypeptides having polymerase activity and methods of use thereof
    1.
    发明授权
    Polypeptides having polymerase activity and methods of use thereof 失效
    具有聚合酶活性的多肽及其使用方法

    公开(公告)号：US07056703B2

    公开(公告)日：2006-06-06

    申请号：US09948369

    申请日：2001-09-06

    申请人： Walter Callen ,  Eric J. Mathur ,  Jay Short

    发明人： Walter Callen ,  Eric J. Mathur ,  Jay Short

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12N9/12

    CPC分类号： C12N9/1252 ,  A01K2217/05

    摘要： The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90° C. up to 113° C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of designing new thermostable DNA polymerases and methods of use thereof are also provided. The polymerases have increased activity and stability at increased pH and temperature.

    摘要翻译： 本发明涉及聚合酶活性温度在90℃至113℃范围内的热稳定聚合酶，例如源自富马酸火焰菌的那些，以及编码聚合酶的多核苷酸。此外，设计新的耐热DNA聚合酶的方法 还提供了其使用方法。 聚合酶在增加的pH和温度下具有增加的活性和稳定性。

    Method of making a protein polymer and uses of the polymer
    2.
    发明授权
    Method of making a protein polymer and uses of the polymer 失效
    制备蛋白质聚合物的方法和聚合物的用途

    公开(公告)号：US07459172B2

    公开(公告)日：2008-12-02

    申请号：US09997807

    申请日：2001-11-30

    申请人： Jay Short ,  Eric J. Mathur ,  W. Michael Lafferty ,  Nelson Barton ,  Kevin Chow

    发明人： Jay Short ,  Eric J. Mathur ,  W. Michael Lafferty ,  Nelson Barton ,  Kevin Chow

    IPC分类号： A61K31/74 ,  A61K38/16

    CPC分类号： B82Y30/00 ,  A61K9/0092 ,  A61K9/1274 ,  C07K14/195

    摘要： A polymer is prepared by self-assembly of a plurality of monomeric polypeptide units. The polymer tends to form a nanotube and is capable of encapsulating a particular drug molecule. Once encapsulated in the polymer of the present invention, the drug molecule may be delivered to a particular location of human body to effectively cure a disease or treat a symptom.Generally, the monomeric polypeptide unit of the present invention has a sequence found in Pyrodictium abyssi, a microorganism that produces an extracellular network having hollow protein tubes, or a sequence substantially identical thereto. The monomeric polypeptide may be mass produced using recombinant biotechnologies and be polymerized into the polymer of the present invention. One or more additional targeting vector may be attached to the monomeric polypeptide unit or the polymer to facilitate the targeting of the drug molecule that may be held there within. The sequence contained in the monomeric polypeptide unit may be further optimized using one or more technique selected from Gene Site Saturation Mutagenesis and GeneReasembly™.

    摘要翻译： 通过多个单体多肽单元的自组装制备聚合物。 聚合物倾向于形成纳米管并且能够封装特定的药物分子。 一旦包封在本发明的聚合物中，药物分子可以被递送到人体的特定位置以有效治愈疾病或治疗症状。 通常，本发明的单体多肽单元具有在黑腹果蝇（Pyrodictium abyssi）中发现的序列，该菌株产生具有中空蛋白管的细胞外网络或与其基本相同的序列。 单体多肽可以使用重组生物技术大规模生产并聚合成本发明的聚合物。 一个或多个另外的靶向载体可以连接到单体多肽单元或聚合物，以促进可能在其中保持的药物分子的靶向。 可以使用选自Gene Site Saturation Mutagenesis和GeneReasembly TM的一种或多种技术进一步优化单体多肽单元中所含的序列。

    CHIMERIC CANNULAE PROTEINS AND METHODS FOR MAKING AND USING THEM
    4.
    发明申请
    CHIMERIC CANNULAE PROTEINS AND METHODS FOR MAKING AND USING THEM 审中-公开
    CHIMERIC CANNULAE蛋白质及其制备和使用方法

    公开(公告)号：US20100172968A1

    公开(公告)日：2010-07-08

    申请号：US11929781

    申请日：2007-10-30

    申请人： Jay Short ,  Eric J. Mathur ,  W. Michael Lafferty ,  Nelson Barton ,  Kevin Chow

    发明人： Jay Short ,  Eric J. Mathur ,  W. Michael Lafferty ,  Nelson Barton ,  Kevin Chow

    IPC分类号： A61K9/48 ,  A61K9/10 ,  C12N15/63 ,  C07K14/00 ,  C07K16/00

    CPC分类号： B82Y30/00 ,  A61K9/0092 ,  A61K9/1274 ,  C07K14/195

    摘要： A polymer is prepared by self-assembly of a plurality of monomeric polypeptide units. The polymer tends to form a nanotube and is capable of encapsulating a particular drug molecule. Once encapsulated in the polymer of the present invention, the drug molecule may be delivered to a particular location of human body to effectively cure a disease or treat a symptom.Generally, the monomeric polypeptide unit of the present invention has a sequence found in Pyrodictium abyssi, a microorganism that produces an extracellular network having hollow protein tubes, or a sequence substantially identical thereto. The monomeric polypeptide may be mass produced using recombinant biotechnologies and be polymerized into the polymer of the present invention. One or more additional targeting vector may be attached to the monomeric polypeptide unit or the polymer to facilitate the targeting of the drug molecule that may be held there within. The sequence contained in the monomeric polypeptide unit may be further optimized using one or more technique selected from Gene Site Saturation Mutagenesis and GeneReasembly.

    摘要翻译： 通过多个单体多肽单元的自组装制备聚合物。 聚合物倾向于形成纳米管并且能够封装特定的药物分子。 一旦包封在本发明的聚合物中，药物分子可以被递送到人体的特定位置以有效治愈疾病或治疗症状。 通常，本发明的单体多肽单元具有在黑腹果蝇（Pyrodictium abyssi）中发现的序列，该菌株产生具有中空蛋白管的细胞外网络或与其基本相同的序列。 单体多肽可以使用重组生物技术大规模生产并聚合成本发明的聚合物。 一个或多个另外的靶向载体可以连接到单体多肽单元或聚合物，以促进可能在其中保持的药物分子的靶向。 可以使用选自基因位点饱和诱变和基因重组的一种或多种技术进一步优化单体多肽单元中所含的序列。