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公开(公告)号：US5091300A
公开(公告)日：1992-02-25
申请号：US389207
申请日：1989-08-03
IPC分类号： G01N33/569 , G01N33/576 , G01N33/577
CPC分类号： G01N33/5764 , Y10S435/81 , Y10S435/948 , Y10S436/804 , Y10S436/808 , Y10S436/82
摘要： Anti-hepatitis B virus surface protein (anti-HBS) antibody is adsorbed onto the surface of a substratum. Hepatitis B virus PreS2+S (PreS2+S) protein is then adsorbed onto the same surface through the interaction of the anti-HBS antibody with the "S" portion of the PreS2+S protein. The coated surface is then incubated concomitantly with the test sample and a radiolabelled antibody specific for the "PreS2" portion of the PreS2+S protein.
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公开(公告)号：US4382076A
公开(公告)日：1983-05-03
申请号：US383668
申请日：1982-06-01
IPC分类号： G01N33/576 , G01N33/48 , G01N33/54 , C12Q1/70
CPC分类号： G01N33/5768 , Y10S435/961 , Y10S435/962 , Y10S436/804 , Y10S436/82
摘要： A diagnostic reagent for hepatitis A antibody is prepared by adhering hepatitis A antibody to a surface by non-specific adsorption followed by specific coupling of hepatitis A antigen to the antibody. This reagent is useful in an in vitro assay for hepatitis A antibody.
摘要翻译： 通过非特异性吸附将甲型肝炎抗体粘附到表面，然后将甲型肝炎抗原与抗体特异性偶联来制备用于甲型肝炎抗体的诊断试剂。 该试剂可用于甲型肝炎抗体的体外测定。
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公开(公告)号：US4293538A
公开(公告)日：1981-10-06
申请号：US31712
申请日：1979-04-20
IPC分类号： G01N33/576 , G01N33/48 , A61K43/00 , G01T1/00
CPC分类号： G01N33/5768 , Y10S436/81 , Y10S436/82
摘要： Liquid containing hepatitis A antibody is adsorbed on a surface. The surface is then coated with a proteinaceous material and incubated in the presence of the sample to form a complex of hepatitis A antigen and hepatitis A antibody. The complex is incubated again in the presence of excess radioactively labelled hepatitis A antibody, and the resulting radionuclide is measured.
摘要翻译： 含有甲型肝炎抗体的液体被吸附在表面上。 然后将表面用蛋白质材料包被，并在样品存在下孵育以形成甲型肝炎抗原和甲型肝炎抗体的复合物。 在过量放射性标记的甲型肝炎抗体存在下再次培养该复合物，并测量所得的放射性核素。
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公开(公告)号：US4861706A
公开(公告)日：1989-08-29
申请号：US857695
申请日：1986-04-29
CPC分类号： C12Q1/706 , Y10S435/948
摘要： A quantitative assay for HAV comprises infecting at a temperature of from about 30.degree. to about 37.degree. C. a cell sheet with a dilution series of hepatitis A virus and then adding Newcastle disease virus (NDV) to the infected cell sheet and incubating the infected cell sheet in the presence of NDV at an elevated temperature for a time sufficient to permit a cytopathic effect (CPE) to become manifest, this time typically being at least about 4 days and preferably about 5 days. At an incubation temperature of from about 31.degree. to about 33.degree. C. the absence of CPE indicates the presence of HAV and the presence of CPE indicates the absence of HAV. At an incubation temperature of from about 34.degree. to about 36.degree. C. the absence of CPE indicates the absence of HAV and the presence of CPE indicates the presence of HAV.
摘要翻译： 用于HAV的定量测定包括在约30℃至约37℃的温度下感染具有稀释系列的甲型肝炎病毒的细胞片，然后向感染的细胞片中加入新城疫病毒（NDV）并孵育感染的 细胞片在NDV存在下在升高的温度下持续足以允许细胞病变效应（CPE）变得明显的时间，此时通常为至少约4天，优选约5天。 在约31℃至约33℃的温育温度下，不存在CPE表明存在HAV，而CPE的存在表明不存在HAV。 在约34℃至约36℃的培养温度下，不存在CPE表明不存在HAV，且CPE的存在表明存在HAV。
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公开(公告)号：US4187989A
公开(公告)日：1980-02-12
申请号：US917811
申请日：1978-06-22
摘要： A method and apparatus for recovering embryos from embryonated eggs wherein the egg is cracked open the shell discarded and the contents dropped onto a continuously vibrating screen which separates the embryo from the yolk sac and accompanying fluid. This associated material, which is less cohesive than the embryo, passes through the screen. The screen itself is tilted at a compound angle from the horizontal and its vibrating action causes the embryos to move along the upper surface of the screen toward a discharge chute located at the lower corner of the screen.
摘要翻译： 一种用于从胚胎卵中回收胚胎的方法和装置，其中蛋破裂，将所丢弃的壳开放并将内容物滴落到将卵与卵黄囊和伴随液体分离的连续振动筛上。 这种相对于胚胎粘合力较小的材料通过筛网。 屏幕本身以与水平面的复合角度倾斜，并且其振动作用使胚胎沿着屏幕的上表面朝向位于屏幕下角的排放槽移动。
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公开(公告)号：US4028190A
公开(公告)日：1977-06-07
申请号：US632610
申请日：1975-11-17
摘要： An apparatus and a method for preparing live, sterile cells from tissue are disclosed which enable high yields of viable isolated cells to be obtained with a greatly reduced possibility of contamination of the cells during the process. The apparatus and method allows one to truly mass manufacture isolated cells as contrasted to a small hand operation requiring many hours and dozens of people. The improved apparatus of the invention comprises a shredder device which mechanically shreds the tissue into small pieces of high surface to volume ratio, a processing vessel in which the shredded tissues are contacted with an enzyme solution to break down the intercellular material and thereby separate the cells one from the other and a means for separating the cells from the enzyme solution. Preferably, the means for separating the cells from the enzyme solution is a flow centrifuge which has been specifically designed to reduce shear force inside the bowl during operation. Also disclosed is a process for shredding tissue employing a plurality of moveable blade elements and at least a corresponding number of separated fixed elements. Interposing the tissue between the fixed and rotating elements and then driving the moveable blade elements through the spaces defined by adjacent fixed elements results in the tissue being shredded into small pieces of high surface to volume ratio. This in turn optimizes surface area for contact between the shredded tissue and the enzyme solution. In addition, a completely closed process is disclosed for obtaining cells from the tissue comprising the steps of sequentially shredding the tissue, contacting the shredded tissue at a controlled temperature with an enzyme solution to break down the intercellular material and then separating the isolated cells from the enzyme solution.
摘要翻译： 公开了一种用于从组织制备活的无菌细胞的装置和方法，其使得可以在该过程中大大降低细胞污染的可能性而获得高得多的活的分离的细胞。 该装置和方法允许真正大量制造隔离的细胞，与需要数十小时和数十人的小手操作相反。 本发明的改进的装置包括一个粉碎装置，其将组织机械地切碎成高的表面与体积比的小块;处理容器，其中切碎的组织与酶溶液接触以分解细胞间材料，从而分离细胞 一个来自另一个和用于从酶溶液中分离细胞的方法。 优选地，用于从酶溶液中分离细胞的装置是流动离心机，其已经被专门设计用于在操作期间减小碗内的剪切力。 还公开了一种使用多个可移动叶片元件和至少相应数量的分离的固定元件来切碎组织的方法。 将组织插入固定和旋转元件之间，然后通过由相邻的固定元件限定的空间驱动可移动的刀片元件，导致组织被切碎成高的表面与体积比的小块。 这又优化了切碎的组织和酶溶液之间的接触的表面积。 另外，公开了一种从组织获得细胞的完全封闭的方法，包括以下步骤：顺序地切碎组织，在受控温度下将切碎的组织与酶溶液接触以分解细胞间材料，然后将分离的细胞从 酶溶液。
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公开(公告)号：US4004981A
公开(公告)日：1977-01-25
申请号：US573655
申请日：1975-05-01
摘要： Apparatus for physically removing cells in sterile manner from a disc stack on which the cells have been cultured comprises a substantially shaft-like member having at least one rod-like member mounted at substantially a right angle to the axis of the shaft.
摘要翻译： 用于以消毒方式物理去除细胞已经培养的盘堆叠的细胞的装置包括具有至少一个棒状部件的大致轴状部件，所述杆状部件以与轴的轴线大致成直角的角度安装。
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公开(公告)号：US4217418A
公开(公告)日：1980-08-12
申请号：US18385
申请日：1979-03-09
CPC分类号： A61K35/16 , Y10S530/806 , Y10S530/83
摘要： It has now been found that small size particles may be extracted from a proteinaceous liquid at a low flow rate in flow-centrifuge operation by precooling the liquid before it enters the centrifuge and by employing a multi-step gradient.
摘要翻译： 现在已经发现，通过在液体进入离心机之前预冷却并通过采用多步梯度，可以在流离心机操作中以低流速从蛋白质液体中提取小尺寸颗粒。
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公开(公告)号：US4242457A
公开(公告)日：1980-12-30
申请号：US13494
申请日：1979-02-21
摘要： Automated means are provided to harvest virus at a time when the yield is optimized and store the harvest with minimum loss thereby eliminating difficulties when the optimum yield time does not fall within normal working hours, enhancing sterility by limiting human contact and enhancing stability of the product.
摘要翻译： 提供自动装置以在产量优化的时候收获病毒，并以最小的损失存储收获，从而在最佳产量时间不在正常工作时间内消除困难，通过限制人的接触和增强产品的稳定性来增强不育 。
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公开(公告)号：US4319271A
公开(公告)日：1982-03-09
申请号：US970722
申请日：1978-12-18
CPC分类号： C12Q1/70 , G01N21/253
摘要： A method for determining the end point in a viral assay comprisingelectronically scanning a microtiter plate to obtain a plurality of data points from each well,transmitting the data points to a digital computer programed to analyze the data points from each well to determine whether each well is positive or negative for CPE in accordance with predetermined parameters, andcalculating the viral titer from the positive or negative results for each well.
摘要翻译： 一种用于确定病毒测定中的终点的方法，包括电子扫描微量滴定板以从每个孔获得多个数据点，将数据点传送到数字计算机，该数字计算机被编程以分析来自每个孔的数据点，以确定每个孔 根据预定参数对于CPE是阳性或阴性，并且从每个孔的阳性或阴性结果计算病毒滴度。
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