摘要:
The present invention relates to methods for preparing an artificial immune system. The artificial immune system comprises a cell culture comprising a three-dimensional matrix comprising lymphoid tissue, a three-dimensional matrix comprising epithelial and/or endothelial cells, and diseased cells. The artificial immune system of the present invention can be used for in vitro testing of vaccines, adjuvants, immunotherapy candidates, cosmetics, drugs, biologics and other chemicals.
摘要:
Single-use long-life faucet mounted water filtration devices are disclosed. A bathroom water filtration device having two outlets for filtered water is disclosed. A rotatable fountain head adjustable in three axes is included for use in the bathroom water filtration device. Press control of the water expelled from the fountain head is controlled by a valve arrangement which divides the flow between the two filtered water outlets. The water filtration device is of unibody construction formed by ultrasonically welding certain parts thereof together. The filter housing end cap includes stops to insure that the filter assembly does not rotate. Since the devices disclosed are disposable, no filter replacement or other maintenance is performed. A gate, magnet(s), sensor and electronics provide an indication of filter performance enabling disposal of the water filtration device and installation of a new device. A kitchen water filtration device is larger than the bathroom device. Both the kitchen and bathroom water filtration devices are small and are mounted behind the faucet connection so as to facilitate full utilization of the sink or wash basin
摘要:
Single-use long-life faucet mounted water filtration devices are disclosed. A bathroom water filtration device having two outlets for filtered water is disclosed. Additionally, a fountain head is included for use in the bathroom water filtration device. The water filtration device is of unibody construction formed by ultrasonically welding certain parts thereof together. Since the devices disclosed are disposable, no filter replacement or other maintenance is performed. A gate, magnet, sensor and electronics provide an indication of filter performance enabling disposal of the water filtration device and installation of a new device. A kitchen water filtration device is larger than the bathroom device. Both the kitchen and bathroom water filtration devices are small and are mounted behind the faucet connection so as to facilitate full utilization of the sink or wash basin.
摘要:
Single-use long-life faucet mounted water filtration devices are disclosed. A bathroom water filtration device having two outlets for filtered water is disclosed. Additionally, a fountain head is included for use in the bathroom water filtration device. The water filtration device is of unibody construction formed by ultrasonically welding certain parts thereof together. Since the devices disclosed are disposable, no filter replacement or other maintenance is performed. A gate, magnet, sensor and electronics provide an indication of filter performance enabling disposal of the water filtration device and installation of a new device. A kitchen water filtration device is larger than the bathroom device. Both the kitchen and bathroom water filtration devices are small and are mounted behind the faucet connection so as to facilitate full utilization of the sink or wash basin.
摘要:
Single-use long-life faucet mounted water filtration devices are disclosed. A bathroom water filtration device having two outlets for filtered water is disclosed. Additionally, a fountain head is included for use in the bathroom water filtration device. The water filtration device is of unibody construction formed by ultrasonically welding certain parts thereof together. Since the devices disclosed are disposable, no filter replacement or other maintenance is performed. A gate, magnet, sensor and electronics provide an indication of filter performance enabling disposal of the water filtration device and installation of a new device. A kitchen water filtration device is larger than the bathroom device. Both the kitchen and bathroom water filtration devices are small and are mounted behind the faucet connection so as to facilitate full utilization of the sink or wash basin.
摘要:
The present invention directs to compositions and methods for modulating immune system. One aspect of the present invention relates to a composition comprising FADD-dependent signaling pathway modulators. Another aspect of the present invention relates to biodegradable microparticles, such as a chitosan microparticle, or PLGA/PEI microparticle, designed to deliver nucleic acids and/or proteins, such as FADD-dependent signaling pathway modulators, to boost different pathways of an immune response. Another aspect of the present invention relates to the method of making biodegradable microparticles. The further aspect of the present invention relates to the use of the chitosan and other polycationic microparticles to deliver FADD-dependent signaling pathway modulators to modulate immune system for the prevention and/or treatment infectious diseases and cancers.
摘要:
The trash container for collecting trash may include an outer container having a first cavity and an inner container being connected to the outer container being positioned within the first cavity. The outer container may include a bottom first wall opposing first walls and a back first wall to define the first cavity. The inner container may include a bottom inner wall, the opposing inner sidewalls and aback inner wall to define a second cavity. The cavity between walls of outer an inner would be created. A cavity would be created in between the inner walls also.
摘要:
The present invention incorporates germinal centers (GCs) into three-dimensional (3D) engineered tissue constructs (ETCs). In an embodiment, we have incorporated the GC in the design of an artificial immune system (AIS) to examine immune responses to vaccines and other compounds. Development of an in vitro GC adds functionality to an AIS, in that it enables generation of an in vitro human humoral response by human B lymphocytes that is accurate and reproducible, without using human subjects. The invention also permits evaluation of, for example, vaccines, allergens, and immunogens, and activation of human B cells specific for a given antigen, which can then be used to generate human antibodies. In an embodiment of the present invention the function of the in vitro GC is enhanced by placing FDCs and other immune cells in a 3D ETC; FDCs appear more effective over a longer time (antibody production is sustained for up to about 14 days.
摘要:
Dendritic cells (DCs) for research and clinical applications are typically derived from purified blood monocytes that are cultured in a cocktail of cytokines for a week or more. Because it has been suggested that these cytokine-derived DCs may be deficient in some important immunological functions and might not accurately represent antigen-presenting cell (APC) populations found under physiologic conditions, there is a need for methods that allow the generation of DCs in a more physiologically relevant manner. The present invention comprises a simple and reliable technique for generating large numbers of highly purified DCs, based on a single migration of blood monocytes through endothelial cells that are cultured in, for example, a Transwell® device. The resultant APCs, harvested from the lower Transwell® chamber, resemble other in vitro-generated DC populations in their expression of major histocompatibility (MHC) and costimulatory molecules, ability to phagocytose foreign antigens, and capacity to trigger antigen-specific T cell responses.
摘要:
The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.