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1.发明授权Cellulase variants with improved expression, activity and stability, and use thereof 有权具有改善的表达，活性和稳定性的纤维素酶变体及其用途公开(公告)号：US08679816B2
公开(公告)日：2014-03-25
申请号：US13321928
申请日：2010-06-03
申请人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W. J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
发明人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W. J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有改善的表达，活性和/或稳定性的纤维素酶变体。 还描述了编码纤维素酶变体的核酸，包含纤维素酶变体的组合物及其使用方法。
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2.发明申请CELLULASE VARIANTS WITH IMPROVED EXPRESSION, ACTIVITY AND STABILITY, AND USE THEREOF 有权具有改进的表达，活性和稳定性的纤维素酶变体及其用途公开(公告)号：US20120135500A1
公开(公告)日：2012-05-31
申请号：US13321928
申请日：2010-06-03
申请人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W.J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
发明人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W.J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有改善的表达，活性和/或稳定性的纤维素酶变体。 还描述了编码纤维素酶变体的核酸，包含纤维素酶变体的组合物及其使用方法。
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公开(公告)号：US09447400B2
公开(公告)日：2016-09-20
申请号：US13510902
申请日：2010-11-19
申请人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Wilhelmus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev , Wilhelmus Antonious Hendricus Van Der Kley , Johannes Franciscus Thomas Van Lieshout , Sander Van Stigt Thans , Gudrun Vogtentanz , Mats Sandgren
发明人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Wilhelmus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev , Wilhelmus Antonious Hendricus Van Der Kley , Johannes Franciscus Thomas Van Lieshout , Sander Van Stigt Thans , Gudrun Vogtentanz , Mats Sandgren
CPC分类号： C12N9/2445 , C12N15/52 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
摘要翻译： 本公开通常涉及酶，特别是β-葡糖苷酶变体。 还描述了编码β-葡糖苷酶变体的核酸，包含β-葡糖苷酶变体的组合物，使用β-葡糖苷酶变体的方法和鉴定另外有用的β-葡糖苷酶变体的方法。
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公开(公告)号：US20130143301A1
公开(公告)日：2013-06-06
申请号：US13510902
申请日：2010-11-19
申请人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev
发明人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev
IPC分类号： C12N9/24
CPC分类号： C12N9/2445 , C12N15/52 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
摘要翻译： 本公开通常涉及酶，特别是β-葡糖苷酶变体。 还描述了编码β-葡糖苷酶变体的核酸，包含β-葡糖苷酶变体的组合物，使用β-葡糖苷酶变体的方法和鉴定另外有用的β-葡糖苷酶变体的方法。
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公开(公告)号：US20100192985A1
公开(公告)日：2010-08-05
申请号：US12616097
申请日：2009-11-10
申请人： Wolfgang Aehle , Luis G. Cascao-Pereira , David A. Estell , Frits Goedegebuur , James T. Kellis, JR. , Ayrookaran J. Poulose , Brian F. Schmidt
发明人： Wolfgang Aehle , Luis G. Cascao-Pereira , David A. Estell , Frits Goedegebuur , James T. Kellis, JR. , Ayrookaran J. Poulose , Brian F. Schmidt
CPC分类号： C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/38636 , C11D3/38645 , C11D3/38654 , C12N9/50 , C12N9/54 , C12Y304/21062
摘要： The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
摘要翻译： 本发明提供蛋白质工程方法和丝氨酸蛋白酶变体。 具体地，本发明提供了与参考丝氨酸蛋白酶相比具有一个或多个取代的丝氨酸蛋白酶变体。 此外，本发明提供包含这些丝氨酸蛋白酶变体的组合物。 在一些实施方案中，本发明提供了包含这些丝氨酸蛋白酶变体中的至少一种的清洁组合物。
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公开(公告)号：US08715997B2
公开(公告)日：2014-05-06
申请号：US13178227
申请日：2011-07-07
申请人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
发明人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
IPC分类号： C12N9/42 , C12N9/14 , C12P21/06 , C12P19/34 , C12N15/00 , C12N1/20 , C12N1/00 , C07H21/04 , C07K1/00
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译： 本文描述了H.cecorina CBH2，Cel6A酶的变体。 本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。
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公开(公告)号：US20120040435A1
公开(公告)日：2012-02-16
申请号：US13178227
申请日：2011-07-07
申请人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
发明人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译： 本文描述了H.cecorina CBH2，Cel6A酶的变体。 本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。
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公开(公告)号：US20060205042A1
公开(公告)日：2006-09-14
申请号：US11317110
申请日：2005-12-22
申请人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
发明人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译： 本文描述了H.cecorina CBH2，Cel6A酶的变体。 本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。
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公开(公告)号：US20120067373A1
公开(公告)日：2012-03-22
申请号：US13079126
申请日：2011-04-04
申请人： Philip Frank Souter , Glenn Steven Ward , Frits Goedegebuur , Ayrookaran Joseph Poulose , David A. Estell , Richard R. Bott , Luis Gustavo Cascäo-Pereira
发明人： Philip Frank Souter , Glenn Steven Ward , Frits Goedegebuur , Ayrookaran Joseph Poulose , David A. Estell , Richard R. Bott , Luis Gustavo Cascäo-Pereira
CPC分类号： C12N9/54 , C11D3/33 , C11D3/378 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/38645 , C11D3/38681 , C11D17/043 , C11D17/044 , C11D17/045
摘要： The present invention is in the field of automatic dishwashing detergent compositions, as well as methods of making and using same. In particular, it relates to an automatic dishwashing detergent composition comprising a new protease. The automatic dishwashing detergent composition provides improved cleaning and finishing. In particular the composition of the invention provides better proteinaceous removal at the same level of other proteases available in the market. This also alternatively allows for the use of a lower level of the protease of the invention and therefore a more cost effective composition.
摘要翻译： 本发明涉及自动餐具洗涤剂组合物领域，以及制造和使用它们的方法。 特别地，本发明涉及一种包含新蛋白酶的自动餐具洗涤剂组合物。 自动洗碗洗涤剂组合物提供改进的清洁和整理。 特别地，本发明的组合物在市场上可获得的相同水平的其它蛋白酶中提供更好的蛋白质去除。 这也可以允许使用较低水平的本发明的蛋白酶，因此使用更具成本效益的组合物。
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公开(公告)号：US20110281327A1
公开(公告)日：2011-11-17
申请号：US13085392
申请日：2011-04-12
申请人： Richard R. Bott , Luis Gustavo Cascao-Pereira , David A. Estell , Frits Goedegebuur , Ayrookaran Joseph Poulose
发明人： Richard R. Bott , Luis Gustavo Cascao-Pereira , David A. Estell , Frits Goedegebuur , Ayrookaran Joseph Poulose
CPC分类号： C12N9/54
摘要： The present invention provides variant proteases, compositions comprising such variant proteases, and methods of cleaning comprising such variant proteases.
摘要翻译： 本发明提供了变体蛋白酶，包含这种变体蛋白酶的组合物和包含这种变体蛋白酶的清洁方法。
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