Diffusion-controlling sensor layer
    1.
    发明申请
    Diffusion-controlling sensor layer 审中-公开
    扩散控制传感器层

    公开(公告)号:US20070292912A1

    公开(公告)日:2007-12-20

    申请号:US11893800

    申请日:2007-08-16

    IPC分类号: C12Q1/68

    摘要: The invention relates to a diffusion-controlling sensor layer for detecting the biological effect of substances consisting of a diffusion-controlling matrix and sensors suspended therein. The invention further comprises a method and an apparatus for the location-dependent detection of the biological effect of substances using the sensor layer according to the invention (“imaging” of the spatial distribution of biological activity). A further embodiment of the method according to the invention relates to the coupling of investigations of the biological activity of substances with chromatographic and electrophoretic separation techniques so that biologically active individual substances can be identified directly in mixtures. An additional embodiment of the method according to the invention comprises the direct combination of the detection of the effect via sensor layers with chromatographic and electrophoretic separation techniques which are in turn coupled to spectroscopic techniques. This close coupling of substance separation, detection of effect and spectroscopy directly provides results on the structure of the biologically active substances in a mixture of substances.

    摘要翻译: 本发明涉及一种扩散控制传感器层,用于检测由扩散控制基质和悬浮在其中的传感器组成的物质的生物学效应。 本发明还包括用于使用根据本发明的传感器层(“成像”生物活性的空间分布)的物质的位置相关检测物质的生物学效应的方法和装置。 根据本发明的方法的另一个实施方案涉及物质生物活性的研究与色谱和电泳分离技术的偶联,使得生物活性的个体物质可以直接在混合物中鉴定。 根据本发明的方法的另外的实施例包括通过传感器层检测效应与色谱和电泳分离技术的直接组合,这些技术又耦合到光谱技术。 物质分离的紧密耦合,效应和光谱的检测直接提供了物质混合物中生物活性物质结构的结果。

    Method and device for detecting biologically active substances
    2.
    发明申请
    Method and device for detecting biologically active substances 失效
    用于检测生物活性物质的方法和装置

    公开(公告)号:US20070154978A1

    公开(公告)日:2007-07-05

    申请号:US11711560

    申请日:2007-02-27

    IPC分类号: C12Q1/18 C12N1/20

    摘要: This application claims a method for detecting biologically active substances, comprising the following steps a) providing a support carrying substances to be tested b) providing a suspension containing luminescent microorganisms, c) coating the support with the suspension of microorganisms, d) detecting the biologically active substances on the support by detecting the change in luminescence of the suspension of microorganisms, and e) stimulating the luminescence of the microorganisms before or during detection, and/or f) extending the period of luminescence of the microorganisms by employing substances for regulating and extending the period of luminescence of the microorganisms.

    摘要翻译: 本申请要求一种用于检测生物活性物质的方法,包括以下步骤:a)提供携带待测物质的载体b)提供含有发光微生物的悬浮液,c)用微生物悬浮液涂覆载体,d) 通过检测微生物悬浮液的发光变化,并且e)在检测前或检测期间刺激微生物的发光,和/或f)通过使用用于调节和调节微生物的物质来延长微生物的发光期 延长微生物的发光时间。

    Method and device for detecting biologically active substances
    3.
    发明授权
    Method and device for detecting biologically active substances 失效
    用于检测生物活性物质的方法和装置

    公开(公告)号:US07695900B2

    公开(公告)日:2010-04-13

    申请号:US11711560

    申请日:2007-02-27

    IPC分类号: C12Q1/00 C12Q1/66

    摘要: This application claims a method for detecting biologically active substances, comprising the following steps a) providing a support carrying substances to be tested b) providing a suspension containing luminescent microorganisms, c) coating the support with the suspension of microorganisms, d) detecting the biologically active substances on the support by detecting the change in luminescence of the suspension of microorganisms, and e) stimulating the luminescence of the microorganisms before or during detection, and/or f) extending the period of luminescence of the microorganisms by employing substances for regulating and extending the period of luminescence of the microorganisms.

    摘要翻译: 本申请要求一种用于检测生物活性物质的方法,包括以下步骤:a)提供携带待测物质的载体b)提供含有发光微生物的悬浮液,c)用微生物悬浮液涂覆载体,d) 通过检测微生物悬浮液的发光变化,并且e)在检测前或检测期间刺激微生物的发光,和/或f)通过使用用于调节和调节微生物的物质来延长微生物的发光期 延长微生物的发光时间。

    Method and device for detecting biologically active substances
    4.
    发明授权
    Method and device for detecting biologically active substances 失效
    用于检测生物活性物质的方法和装置

    公开(公告)号:US07183113B2

    公开(公告)日:2007-02-27

    申请号:US10424067

    申请日:2003-04-25

    IPC分类号: G01N33/53

    摘要: The invention claimed herein is a kit for detecting biologically active substances on a support, comprising the luminescent marine bacteria Vibria Fischeri in a form stabilized for transport and storage, one or more cultivation media or the individual components of cultivation media for the microorganism wherein the cultivation media contains 100 mg/l to 500 mg/l of aspartic acid, and optionally additives for extending the period of luminescence selected from N-acylhomoserine lactones, dipeptides, oligopeptides, or their biochemical precursors, and boron compounds.

    摘要翻译: 本文所要求的发明是用于检测载体上的生物活性物质的试剂盒,其包含以稳定运输和储存的形式的发光海洋细菌Vibria Fischeri,一种或多种培养基或微生物培养基的各组分,其中培养 培养基含有100mg / l至500mg / l的天冬氨酸,以及任选的用于延长选自N-酰基高丝氨酸内酯,二肽,寡肽或其生化前体的发光期和硼化合物的添加剂。

    Analytical process for testing mixtures for toxic constituents
    5.
    发明授权
    Analytical process for testing mixtures for toxic constituents 失效
    用于测试有毒成分混合物的分析方法

    公开(公告)号:US06238928B1

    公开(公告)日:2001-05-29

    申请号:US08116382

    申请日:1993-09-02

    IPC分类号: G01N3090

    摘要: The mixture to be tested for toxic components is first separated into fractions by chromatography. The separated fractions are then contacted with luminescent microorganisms in the chromatographic system itself and the toxicity of the fractions is determined by measurement of the bioluminescence. The chromatographic separation may be carried out either by thin layer chromatography or by column liquid chromatography.

    摘要翻译: 待测试有毒成分的混合物首先通过色谱法分离成级分。 然后将分离的级分与色谱系统中的发光微生物本身接触,并通过测量生物发光来测定级分的毒性。 色谱分离可以通过薄层色谱法或通过柱色谱法进行。

    Process for improving the quality of Robusta coffee
    9.
    发明授权
    Process for improving the quality of Robusta coffee 失效
    提高罗布斯塔咖啡质量的过程

    公开(公告)号:US5019413A

    公开(公告)日:1991-05-28

    申请号:US601301

    申请日:1990-10-22

    IPC分类号: A23F5/02 A23F5/04 A23F5/16

    CPC分类号: A23F5/02 A23F5/16

    摘要: The quality of Robusta coffee is increased by treating raw Robusta coffee beans whose water content has been increased to about 30% to 45% with steam at about 135.degree. C. to 140.degree. C. and at a pressure ranging from 3 bar to 4 bar approx. for 60 min to 120 min. This treatment particularly removes the substance responsible for the "earthy" and "musty" aroma note of Robusta coffee, namely the compound 2-methylisoborneol. After the treatment, the beans are processed and roasted in the customary way.

    摘要翻译: 罗布斯塔咖啡的质量通过在约135℃至140℃和约3巴至4巴的压力下处理含水量已增加至约30%至45%的原始罗布斯塔咖啡豆而增加 大约 60分钟至120分钟。 这种处理特别消除了负责罗布斯塔咖啡的“泥土”和“发霉”香气的物质,即化合物2-甲基异山梨醇。 处理后,豆类以常规方式加工和烘烤。