公开(公告)号：US08247184B2

公开(公告)日：2012-08-21

申请号：US13020068

申请日：2011-02-03

申请人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

发明人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

IPC分类号： G01N33/53 ,  G01N33/567 ,  C12N1/00 ,  C12N5/07 ,  C12N5/10

CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/10

摘要： This invention relates to chimeric taste receptors comprising the extracellular portion of one T1R or a variant or fragment thereof, either T1R1 or T1R2, and the transmembrane portion of another T1R or a variant or fragment thereof, either T1R1 or T1R2, preferably associated with a T1R3 polypeptide and a suitable G protein. These chimeric taste receptors and cells which express such chimeric taste receptors are useful in assays for identifying sweet and umami ligands as well in assays for identifying sweet and umami enhancers. Additionally, these chimeric taste receptors and cells which express same can be used to map and determine where specific sweet and umami ligands interact with their respective receptors and to elucidate the mechanism of receptor activation.

摘要翻译： 本发明涉及包含一个T1R的细胞外部分或其变体或片段T1R1或T1R2的嵌合味觉感受器，以及T1R1或其变体或片段的跨膜部分，T1R1或T1R2，优选与T1R3相关 多肽和合适的G蛋白。 这些嵌合味觉受体和表达这种嵌合味觉受体的细胞可用于鉴定甜味和鲜味配体的测定以及用于鉴定甜味和鲜味增强剂的测定中。 此外，这些嵌合味觉受体和表达相同的细胞可以用于绘制和确定特定甜味和鲜味配体与其各自受体相互作用的位置，并阐明受体活化的机制。

公开(公告)号：US20070161053A1

公开(公告)日：2007-07-12

申请号：US11583097

申请日：2006-10-19

申请人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

发明人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

IPC分类号： G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705

CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/10

摘要： This invention relates to chimeric taste receptors comprising the extracellular portion of one T1R or a variant or fragment thereof, either T1R1 or T1R2, and the transmembrane portion of another T1R or a variant or fragment thereof, either T1R1 or T1R2, preferably associated with a T1R3 polypeptide and a suitable G protein. These chimeric taste receptors and cells which express such chimeric taste receptors are useful in assays for identifying sweet and umami ligands as well in assays for identifying sweet and umami enhancers. Additionally, these chimeric taste receptors and cells which express same can be used to map and determine where specific sweet and umami ligands interact with their respective receptors and to elucidate the mechanism of receptor activation.

摘要翻译： 本发明涉及包含一个T 1 R的细胞外部分或其变体或片段，即T 1 R 1或T 1 R 2以及另一个T 1 R或其变体或片段的跨膜部分的嵌合味觉感受器， T 1 R 1或T 1 R 2，优选与T 1 R 3多肽和合适的G蛋白相关。 这些嵌合味觉受体和表达这种嵌合味觉受体的细胞可用于鉴定甜味和鲜味配体的测定以及用于鉴定甜味和鲜味增强剂的测定中。 此外，这些嵌合味觉受体和表达相同的细胞可以用于绘制和确定特定甜味和鲜味配体与其各自受体相互作用的位置，并阐明受体活化的机制。

公开(公告)号：US07906627B2

公开(公告)日：2011-03-15

申请号：US11583097

申请日：2006-10-19

申请人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

发明人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

IPC分类号： C07K1/00 ,  C07K14/00 ,  C07K16/00 ,  C07K17/00 ,  C08H1/00 ,  C07H21/04 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N15/74

CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/10

摘要： This invention relates to chimeric taste receptors comprising the extracellular portion of one T1R or a variant or fragment thereof, either T1R1 or T1R2, and the transmembrane portion of another T1R or a variant or fragment thereof, either T1R1 or T1R2, preferably associated with a T1R3 polypeptide and a suitable G protein. These chimeric taste receptors and cells which express such chimeric taste receptors are useful in assays for identifying sweet and umami ligands as well in assays for identifying sweet and umami enhancers. Additionally, these chimeric taste receptors and cells which express same can be used to map and determine where specific sweet and umami ligands interact with their respective receptors and to elucidate the mechanism of receptor activation.

摘要翻译： 本发明涉及包含一个T1R的细胞外部分或其变体或片段T1R1或T1R2的嵌合味觉感受器，以及T1R1或其变体或片段的跨膜部分，T1R1或T1R2，优选与T1R3相关 多肽和合适的G蛋白。 这些嵌合味觉受体和表达这种嵌合味觉受体的细胞可用于鉴定甜味和鲜味配体的测定以及用于鉴定甜味和鲜味增强剂的测定中。 此外，这些嵌合味觉受体和表达相同的细胞可以用于绘制和确定特定甜味和鲜味配体与其各自受体相互作用的位置，并阐明受体活化的机制。

公开(公告)号：US20100304402A9

公开(公告)日：2010-12-02

申请号：US11583097

申请日：2006-10-19

申请人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

发明人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

IPC分类号： G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705

CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/10

摘要： This invention relates to chimeric taste receptors comprising the extracellular portion of one T1R or a variant or fragment thereof, either T1R1 or T1R2, and the transmembrane portion of another T1R or a variant or fragment thereof, either T1R1 or T1R2, preferably associated with a T1R3 polypeptide and a suitable G protein. These chimeric taste receptors and cells which express such chimeric taste receptors are useful in assays for identifying sweet and umami ligands as well in assays for identifying sweet and umami enhancers. Additionally, these chimeric taste receptors and cells which express same can be used to map and determine where specific sweet and umami ligands interact with their respective receptors and to elucidate the mechanism of receptor activation.

摘要翻译： 本发明涉及包含一个T1R的细胞外部分或其变体或片段T1R1或T1R2的嵌合味觉感受器，以及T1R1或其变体或片段的跨膜部分，T1R1或T1R2，优选与T1R3相关 多肽和合适的G蛋白。 这些嵌合味觉受体和表达这种嵌合味觉受体的细胞可用于鉴定甜味和鲜味配体的测定以及用于鉴定甜味和鲜味增强剂的测定中。 此外，这些嵌合味觉受体和表达相同的细胞可以用于绘制和确定特定甜味和鲜味配体与其各自受体相互作用的位置，并阐明受体活化的机制。

公开(公告)号：US20110223618A1

公开(公告)日：2011-09-15

申请号：US13020068

申请日：2011-02-03

申请人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

发明人： Xiaodong Li ,  Feng Zhang ,  Hong Xu ,  Qing Li

IPC分类号： G01N33/566

CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/10

摘要： This invention relates to chimeric taste receptors comprising the extracellular portion of one T1R or a variant or fragment thereof, either T1R1 or T1R2, and the transmembrane portion of another T1R or a variant or fragment thereof, either T1R1 or T1R2, preferably associated with a T1R3 polypeptide and a suitable G protein. These chimeric taste receptors and cells which express such chimeric taste receptors are useful in assays for identifying sweet and umami ligands as well in assays for identifying sweet and umami enhancers. Additionally, these chimeric taste receptors and cells which express same can be used to map and determine where specific sweet and umami ligands interact with their respective receptors and to elucidate the mechanism of receptor activation.

摘要翻译： 本发明涉及包含一个T1R的细胞外部分或其变体或片段T1R1或T1R2的嵌合味觉感受器，以及T1R1或其变体或片段的跨膜部分，T1R1或T1R2，优选与T1R3相关 多肽和合适的G蛋白。 这些嵌合味觉受体和表达这种嵌合味觉受体的细胞可用于鉴定甜味和鲜味配体的测定以及用于鉴定甜味和鲜味增强剂的测定中。 此外，这些嵌合味觉受体和表达相同的细胞可以用于绘制和确定特定甜味和鲜味配体与其各自受体相互作用的位置，并阐明受体活化的机制。

公开(公告)号：US08318447B2

公开(公告)日：2012-11-27

申请号：US13094974

申请日：2011-04-27

申请人： Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang

发明人： Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang

IPC分类号： G01N33/567 ,  C07K14/705

CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/00 ,  G01N2500/10 ,  Y10S514/974

摘要： The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter compounds, i.e., chlorogenic lactone compounds that contribute at least partially to the bitter taste of many coffee beverages. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors by chlorogenic lactones and related compounds and which may be used as additives and/or removed from foods, beverages and medicinals in order to modify (block) T2R-associated bitter taste. A preferred embodiment is the use of the identified compounds as additives in coffee and coffee-flavored foods, beverages and medicinals.

摘要翻译： 本发明涉及T2R味觉受体家族中的特定人味受体对特定苦味化合物的反应的发现，即，至少部分地促成许多咖啡饮料的苦味的绿色内酯化合物。 本发明还涉及这些受体在测定中用于鉴定调节绿色内酯和相关化合物来调节这些味觉受体的活化并可以用作添加剂和/或从食品，饮料和药物中去除的配体的用途，以便 修改（阻断）T2R相关的苦味。 优选的实施方案是使用所鉴定的化合物作为咖啡和咖啡风味食品，饮料和药物中的添加剂。

公开(公告)号：US07915003B2

公开(公告)日：2011-03-29

申请号：US12166980

申请日：2008-07-02

申请人： Xiaodong Li ,  Hong Xu ,  Huixian Tang ,  Qing Li

发明人： Xiaodong Li ,  Hong Xu ,  Huixian Tang ,  Qing Li

IPC分类号： G01N33/566 ,  C07K14/705

CPC分类号： G01N33/74 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02

摘要： The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter ligands, i.e., acetaminophen, ranitidine, strychnine and denatonium. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors and which may be used as additives in foods, beverages and medicinals for modifying (blocking) T2R-associated bitter taste.

摘要翻译： 本发明涉及T2R味觉受体家族中的特定人味受体对特定苦味配体即对乙酰氨基酚，雷尼替丁，士的宁和丹那铵的反应的发现。 本发明还涉及这些受体在用于鉴定调节这些味觉受体的活化的配体的测定中的用途，并且其可用作用于改变（阻断）T2R相关苦味的食品，饮料和药物中的添加剂。

公开(公告)号：US08715946B2

公开(公告)日：2014-05-06

申请号：US13019580

申请日：2011-02-02

申请人： Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang ,  Alexey Pronin

发明人： Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang ,  Alexey Pronin

IPC分类号： G01N33/567 ,  C07K14/705

CPC分类号： G01N33/566 ,  G01N33/5008 ,  G01N33/5023 ,  G01N2500/00 ,  G01N2500/10

摘要： The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter compounds. Also, the invention relates to the discovery of specific hT2R9 alleles and their disparate activity in functional assays with the same bitter ligands. The invention further relates to the use of these T2R receptors in assays for identifying ligands that modulate the activation of these taste receptors by specific bitter ligands and related compounds. These compounds may be used as additives and/or removed from foods, beverages, cosmetics and medicinals in order to modify (block) T2R-associated bitter taste. Also T2R ligands may be used as therapeutics to treat and modulate T2R associated gastrointestinal and metabolic functions as well as treat gastrointestinal and metabolic diseases such as eating disorders, food sensing, food absorption, obesity, diabetes, Crohn's disease, celiac disease, et al.

摘要翻译： 本发明涉及T2R味觉受体家族中的特定人味受体对特定苦味化合物的反应的发现。 此外，本发明涉及特异性hT2R9等位基因的发现及其在具有相同苦味配体的功能测定中的不同活性。 本发明还涉及这些T2R受体在用于鉴定通过特定的苦配体和相关化合物调节这些味觉受体的活化的配体的测定中的用途。 这些化合物可以用作添加剂和/或从食品，饮料，化妆品和药物中去除，以便改变（阻断）T2R相关的苦味。 也可以使用T2R配体作为治疗和调节T2R相关胃肠和代谢功能以及治疗胃肠道和代谢疾病如饮食失调，食物感觉，食物吸收，肥胖，糖尿病，克罗恩病，乳糜泻等的治疗剂。

公开(公告)号：US08273542B2

公开(公告)日：2012-09-25

申请号：US13052578

申请日：2011-03-21

申请人： Xiaodong Li ,  Hong Xu ,  Hulxian Tang ,  Qing Li

发明人： Xiaodong Li ,  Hong Xu ,  Hulxian Tang ,  Qing Li

IPC分类号： G01N33/53 ,  G01N33/566

CPC分类号： G01N33/74 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02

摘要： The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter ligands, i.e., acetaminophen, ranitidine, strychnine and denatonium. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors and which may be used as additives in foods, beverages and medicinals for modifying (blocking) T2R-associated bitter taste.

摘要翻译： 本发明涉及T2R味觉受体家族中的特定人味受体对特定苦味配体即对乙酰氨基酚，雷尼替丁，士的宁和丹那铵的反应的发现。 本发明还涉及这些受体在用于鉴定调节这些味觉受体的活化的配体的测定中的用途，并且其可用作用于改变（阻断）T2R相关苦味的食品，饮料和药物中的添加剂。

公开(公告)号：US07939276B2

公开(公告)日：2011-05-10

申请号：US11455693

申请日：2006-06-20

申请人： Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang

发明人： Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang

IPC分类号： G01N33/567 ,  C07K14/705

CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/00 ,  G01N2500/10 ,  Y10S514/974

摘要： The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter compounds, i.e., chlorogenic lactone compounds that contribute at least partially to the bitter taste of many coffee beverages. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors by chlorogenic lactones and related compounds and which may be used as additives and/or removed from foods, beverages and medicinals in order to modify (block) T2R-associated bitter taste. A preferred embodiment is the use of the identified compounds as additives in coffee and coffee-flavored foods, beverages and medicinals.

摘要翻译： 本发明涉及T2R味觉受体家族中的特定人味受体对特定苦味化合物的反应的发现，即，至少部分地促成许多咖啡饮料的苦味的绿色内酯化合物。 本发明还涉及这些受体在测定中用于鉴定调节绿色内酯和相关化合物来调节这些味觉受体的活化并可以用作添加剂和/或从食品，饮料和药物中去除的配体的用途，以便 修改（阻断）T2R相关的苦味。 优选的实施方案是使用所鉴定的化合物作为咖啡和咖啡风味食品，饮料和药物中的添加剂。