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    SYSTEMS AND METHODS FOR PROTEIN PRODUCTION
    2.
    发明申请
    SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 审中-公开
    蛋白质生产的系统和方法

    公开(公告)号：US20120322104A1

    公开(公告)日：2012-12-20

    申请号：US13446649

    申请日：2012-04-13

    申请人： Yijie GAO ,  Nicloe M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    发明人： Yijie GAO ,  Nicloe M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    IPC分类号： C12N5/10 ,  C12P21/00 ,  C12N15/79 ,  C12N5/16

    CPC分类号： C07K14/4705 ,  A01K2217/075 ,  C07K14/51 ,  C12P21/02

    摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6-mediated UPR pathway.

    摘要翻译： 本发明涉及生产目标蛋白质的系统和方法。 本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。 一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。 多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。 在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XBP1或ATF6介导的UPR途径的组分或调节剂。

    Systems and methods for protein production
    3.
    发明授权
    Systems and methods for protein production 有权
    蛋白质生产的系统和方法

    公开(公告)号：US08173394B2

    公开(公告)日：2012-05-08

    申请号：US12419018

    申请日：2009-04-06

    申请人： Yijie Gao ,  Nicole M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    发明人： Yijie Gao ,  Nicole M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    IPC分类号： C12P21/06 ,  C12N5/00

    CPC分类号： C07K14/4705 ,  A01K2217/075 ,  C07K14/51 ,  C12P21/02

    摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XB1- or ATF6-mediated UPR pathway.

    摘要翻译： 本发明涉及生产目标蛋白质的系统和方法。 本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。 一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。 多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。 在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XB1或ATF6介导的UPR途径的组分或调节剂。

    SYSTEMS AND METHODS FOR PROTEIN PRODUCTION
    4.
    发明申请
    SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 有权
    蛋白质生产的系统和方法

    公开(公告)号：US20090221035A1

    公开(公告)日：2009-09-03

    申请号：US12419018

    申请日：2009-04-06

    申请人： Yijie Gao ,  Nicole M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    发明人： Yijie Gao ,  Nicole M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    IPC分类号： C12P21/00 ,  C12N5/04 ,  C12N5/06 ,  A01K67/027 ,  C12N15/00

    CPC分类号： C07K14/4705 ,  A01K2217/075 ,  C07K14/51 ,  C12P21/02

    摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XB1- or ATF6-mediated UPR pathway.

    摘要翻译： 本发明涉及生产目标蛋白质的系统和方法。 本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。 一方面，本发明的特征在于遗传工程改造的细胞，其包含一个或多个编码（1）目的蛋白质的重组表达盒，和（2）在细胞的未折叠的蛋白质应答（UPR）途径中有功能的多肽。 多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。 在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XB1或ATF6介导的UPR途径的组分或调节剂。

    Systems and methods for protein production
    5.
    发明授权
    Systems and methods for protein production 有权
    蛋白质生产的系统和方法

    公开(公告)号：US07514237B2

    公开(公告)日：2009-04-07

    申请号：US11217137

    申请日：2005-09-01

    申请人： Yijie Gao ,  Nicole M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    发明人： Yijie Gao ,  Nicole M. Piche ,  Mei Geng ,  Stephen H. Herrmann ,  Xiaotian Zhong ,  Ronald Kriz ,  Zhijian Lu

    IPC分类号： C12P21/06 ,  C12N1/20

    CPC分类号： C07K14/4705 ,  A01K2217/075 ,  C07K14/51 ,  C12P21/02

    摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6-mediated UPR pathway.

    摘要翻译： 本发明涉及生产目标蛋白质的系统和方法。 本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。 一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。 多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。 在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XBP1或ATF6介导的UPR途径的组分或调节剂。

    Methods of labeling transiently expressed proteins in large-scale eukaryotic cell cultures
    6.
    发明申请
    Methods of labeling transiently expressed proteins in large-scale eukaryotic cell cultures 审中-公开
    在大规模真核细胞培养物中标记瞬时表达的蛋白质的方法

    公开(公告)号：US20070161047A1

    公开(公告)日：2007-07-12

    申请号：US11651135

    申请日：2007-01-09

    申请人： Xiaotian Zhong ,  Ronald Kriz

    发明人： Xiaotian Zhong ,  Ronald Kriz

    IPC分类号： G01N33/53 ,  C12P21/06 ,  C12N1/18 ,  C12N5/06 ,  C07K14/47

    CPC分类号： C07K1/13 ,  C12N9/12 ,  C12P21/02

    摘要： The present invention is based on observations that transiently transfecting large-scale eukaryotic cell cultures with a polynucleotide encoding a protein of interest can be used to rapidly produce the large quantities of labeled proteins required for various biochemical techniques such as spectroscopy, microscopy, and crystallography, and applications including protein structure determination, protein tracing and/or localization, diagnostic and therapeutic applications, and affinity experiments. Thus, the present invention provides methods for rapidly producing large quantities of labeled proteins by using transient transfection of large-scale eukaryotic cell cultures, which are then grown in a chemically defined labeling medium that includes labeled amino acids. The present invention is also directed to methods of using the labeled proteins produced by the novel labeling methods for use in various techniques.

    摘要翻译： 本发明基于以编码目的蛋白质的多核苷酸瞬时转染大规模真核细胞培养物的观察结果，可以用于快速产生各种生物化学技术如光谱学，显微镜和晶体学所需的大量标记蛋白质， 以及包括蛋白质结构测定，蛋白质追踪和/或定位，诊断和治疗应用以及亲和力实验的应用。 因此，本发明提供了通过使用大规模真核细胞培养物的瞬时转染来快速产生大量标记的蛋白质的方法，然后将其在包括标记的氨基酸的化学学定义的标记培养基中生长。 本发明还涉及使用通过新型标记方法产生的标记蛋白用于各种技术的方法。