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公开(公告)号：US6138082A
公开(公告)日：2000-10-24
申请号：US993483
申请日：1997-12-18
IPC分类号： G01J3/02 , G01J3/28 , G01J3/45 , G01N21/27 , G01N21/35 , G01N30/62 , G01N30/74 , G01N30/86 , G01R35/00
CPC分类号： G01J3/28 , G01N21/274 , G01N30/62 , G01N2030/626 , G01N2030/743
摘要： For conversion of spectral information of an FTIR spectrometric instrument for comparison with that of a dispersion instrument, a first standard function is selected for spectral line shape for the first instrument, and a second standard function for line shape is selected for the second instrument. A conversion factor is computed for converting the first standard function to the second standard function. In ordinary operations, first spectral information is obtained with the first instrument for a first sample, and second spectral information is obtained with the second instrument for a second sample. The conversion factor is applied to the first spectral information to effect converted information, and the converted information is compared with the second spectral information. Such conversion also is applied between chromatographic instruments.
摘要翻译： 为了将FTIR光谱仪的光谱信息转换成与分散仪器相比较的光谱信息，为第一仪器的谱线形状选择第一标准函数，为第二仪器选择线形的第二标准函数。 计算用于将第一标准函数转换为第二标准函数的转换因子。 在普通操作中，用第一样品的第一仪器获得第一光谱信息，并且用第二样品的第二仪器获得第二光谱信息。 将转换因子应用于第一光谱信息以实现转换的信息，并将转换的信息与第二光谱信息进行比较。 这种转化也适用于色谱仪器。
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公开(公告)号：US6049762A
公开(公告)日：2000-04-11
申请号：US993482
申请日：1997-12-18
申请人： Alan M. Ganz , Yongdong Wang , David H. Tracy , Robert A. Hoult , Jerry E. Cahill , David A. Huppler
发明人： Alan M. Ganz , Yongdong Wang , David H. Tracy , Robert A. Hoult , Jerry E. Cahill , David A. Huppler
CPC分类号： G01J3/453 , G01J2003/2866
摘要： Standardization is achieved for FTIR spectrometric instruments that effect an intrinsic distortion in spectral information, the distortion being associated with an aperture size. An idealized function of spectral line shape is specified. With a small calibration aperture, spectral data is obtained for a basic sample having known "true" spectral data, and standard spectral data also is obtained for a standard sample. With a larger, normal sized aperture, standard spectral data is obtained again for the calibration sample. A transformation factor, that is a function of this data and the standardized function, is applied to spectral data for test samples to effect standardized information. In another embodiment, the standard sample has known true spectral data, and the basic sample is omitted. In either case, the transformation factor is applied to the sample data in logarithm form, the antilogarithm of the result effects the standardized information.
摘要翻译： 对于影响光谱信息中的固有失真的FTIR光谱仪器实现标准化，失真与孔径尺寸相关联。 规定了光谱线形状的理想化功能。 使用小的校准孔径，获得具有已知“真实”光谱数据的基本样品的光谱数据，并且还为标准样品获得标准光谱数据。 对于较大的正常尺寸的孔径，再次为校准样品获得标准光谱数据。 作为该数据和标准化函数的函数的变换因子被应用于测试样本的光谱数据以实现标准化信息。 在另一个实施例中，标准样品具有已知的真实光谱数据，并省略了基本样品。 在任一种情况下，转换因子以对数形式应用于样本数据，结果的反对数效应影响标准化信息。
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公开(公告)号：US06029115A
公开(公告)日：2000-02-22
申请号：US940575
申请日：1997-09-30
申请人： David H. Tracy , Alan M. Ganz , Yongdong Wang , David A. Huppler , Juan C. Ivaldi , Christopher B. Hanna
发明人： David H. Tracy , Alan M. Ganz , Yongdong Wang , David A. Huppler , Juan C. Ivaldi , Christopher B. Hanna
CPC分类号： G01J3/28 , G01J3/2803 , G01J3/457 , G01J2003/2866
摘要： A spectrometric instrument includes a detector with detecting subarrays on small portions of the surface. Spectral data are acquired for selected subarrays at a first time for a drift standard, and compared to a zero position to obtain first offset data. Data are acquired similarly at a second time to obtain second offset data. The offset data are utilized to obtain a spectral shift for any subarray position at any selected time. The shift is applied to a matrix model used for converting test data to compositional information. Archive data for the model is obtained in the foregoing manner, using slit scanning in the instrument to achieve sub-increments smaller than the detector pixel size, with a procedure to assure that there is an integral number of scanning steps across one pixel. The drift standard may be chemical analytes, or an optical interference element producing fringes related to spectral positions in each subarray. A procedure is used to identify the fringe peaks to spectral position, with temperature correction.
摘要翻译： 光谱测量仪器包括一个检测器，在表面的小部分具有检测子阵列。 针对漂移标准在第一时间针对所选择的子阵列采集光谱数据，并与零位置进行比较以获得第一偏移数据。 在第二时间类似地获取数据以获得第二偏移数据。 偏移数据用于在任何选定的时间获得任何子阵列位置的光谱偏移。 该转移被应用于用于将测试数据转换成组合信息的矩阵模型。 以上述方式获得用于模型的存档数据，使用仪器中的狭缝扫描来实现小于检测器像素尺寸的次增量，以确保跨越一个像素存在整数个扫描步骤的过程。 漂移标准可以是化学分析物，或者产生与每个子阵列中的光谱位置相关的条纹的光学干涉元件。 使用一个程序来确定边缘峰到光谱位置，并进行温度校正。
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4.发明授权公开(公告)号：US06430513B1
公开(公告)日：2002-08-06
申请号：US09479642
申请日：2000-01-07
申请人： Yongdong Wang , David H. Tracy , Paul G. Saviano , Alan M. Ganz , Koichi Nishikida , Gitesh Kumar
发明人： Yongdong Wang , David H. Tracy , Paul G. Saviano , Alan M. Ganz , Koichi Nishikida , Gitesh Kumar
IPC分类号： G01N3100
CPC分类号： A61B5/6814 , A61B5/14546 , A61B5/14553 , G01N21/49
摘要： Constituents such as oxy- and deoxy-hemoglobin are monitored non-invasively in an animal organ such as a brain with a spectrometric instrument by passing radiation through the organ. Concentrations are computed from the spectral intensities and from a statistical correlation model. To predetermine the correlation model, the procedures are effected for a plurality of organs of a same type with each organ having established concentrations of the selected constituents, and the correlation model is statistically determined from the concentrations and corresponding intensities. For more accuracy computations are normalized to path length which may be determined by utilizing several discrete wavelengths with RF modulations.
摘要翻译： 通过使辐射通过器官，通过光谱仪器在诸如脑的动物器官中非侵入性地监测诸如氧和血红蛋白的成分。 浓度从光谱强度和统计相关模型计算。 为了预先确定相关模型，对于具有确定的所选组分浓度的每个器官，对于多个相同类型的器官进行该程序，并且根据浓度和相应的强度统计地确定相关模型。 为了更准确的计算，归一化到可以通过利用具有RF调制的几个离散波长来确定的路径长度。
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公开(公告)号：US5928907A
公开(公告)日：1999-07-27
申请号：US752973
申请日：1996-12-02
申请人： Timothy M. Woudenberg , Kevin S. Bodner , Charles R. Connell , Alan M. Ganz , Lincoln J. McBride , Paul G. Saviano , John Shigeura , David H. Tracy , Eugene F. Young , Linda G. Lee
发明人： Timothy M. Woudenberg , Kevin S. Bodner , Charles R. Connell , Alan M. Ganz , Lincoln J. McBride , Paul G. Saviano , John Shigeura , David H. Tracy , Eugene F. Young , Linda G. Lee
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68 , G01N21/03 , G01N21/64 , G01N21/75 , G01N21/78 , G01N33/50 , G01N33/58
CPC分类号： G01N21/6428 , C12Q1/6818 , C12Q1/6851 , C12Q1/686 , G01N21/0303 , G01N2021/6484
摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.
摘要翻译： 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例 在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。
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公开(公告)号：US5303165A
公开(公告)日：1994-04-12
申请号：US834448
申请日：1992-02-12
申请人： Alan M. Ganz , David H. Tracy , Robert A. Hoult
发明人： Alan M. Ganz , David H. Tracy , Robert A. Hoult
CPC分类号： G01J3/2803 , G01J3/28 , G01J2003/2866
摘要： A spectrometric instrument which exhibits an intrinsic profile for a sharp spectral line produces profile data for narrow spectral lines. The spectral lines are effected with a high finesse etalon of gold coated polymer. A transformation filter is computed for transforming the profile data to a gaussian profile. A wavelength calibration is combined with the filter to effect a correction matrix which is applied to sample data to generate calibrated standardized data. Iteratively a correction matrix is applied to calibration data to generate standardized calibration data which is utilized for the wavelength calibration. Calibration is effected with an optical standard, an interference etalon and a fringe formula. Etalon effective thickness is first estimated and then precisely determined so that fringe peaks calibrate wavelength.
摘要翻译： 显示锋利光谱线的固有分布的光谱仪可以产生窄谱线的谱图数据。 光谱线用金涂层聚合物的高精密标准具实现。 计算转换滤波器以将简档数据变换为高斯分布。 波长校准与滤波器组合以实现校正矩阵，其被应用于采样数据以产生校准的标准化数据。 迭代地将校正矩阵应用于校准数据以产生用于波长校准的标准化校准数据。 用光学标准，干涉标准具和边缘公式进行校准。 首先估计Etalon有效厚度，然后精确确定边缘峰值校准波长。
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公开(公告)号：US5229838A
公开(公告)日：1993-07-20
申请号：US823631
申请日：1992-01-21
申请人： Alan M. Ganz , David H. Tracy
发明人： Alan M. Ganz , David H. Tracy
CPC分类号： G01J1/08 , G01N21/274
摘要： To calibrate a photodetector, a rotating disk with a slot is disposed in a light beam with decreasing speed from a defined maximum rotational speed to a defined minimum speed, while magnitudes and times of signals are read out and stored. Vernier pairs of signals occur in adjacent readout intervals, and non-vernier signals exclude the verniers. Readout times for verniers are used to estimate a preliminary function of rotations versus time. From the function are estimated an occurrence time for each pair and period of disk rotation at the time. Vernier fraction is the ratio of one signal in the pair to the sum of the pair. A time offset is the product of vernier fraction, slot fraction of the disk and the estimated period. Occurrence times corrected with the time offset are utilized to fit a corrected function of disk rotations versus time. Points of time for the non-vernier signals are determined from the corrected function, each point corresponding to disk rotations to a corresponding non-vernier signal. These points of time are employed with corresponding signals for linearly calibrating the photodetector.
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公开(公告)号：US07656521B2
公开(公告)日：2010-02-02
申请号：US12119239
申请日：2008-05-12
IPC分类号： G01J3/00
摘要： In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, andthe transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.
摘要翻译： 在分光过程中，刺激用于产生测试谱线或样品中包含的至少一种组分的光谱的样品，并且使用透射和/或发射的电磁波来产生测试光谱线或光谱。 为了改进这种光谱过程，使得补偿了改变光谱线的形状和/或出现的某些参数的变化，已知比较材料的比较光谱线或光谱基本上在 与样品相同的参数。 将比较谱线或频谱与理想的比较谱线或频谱进行比较，以计算传递函数，并将传递函数应用于测试谱线或频谱，以便计算校正的测试谱线或频谱。
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公开(公告)号：US07405821B2
公开(公告)日：2008-07-29
申请号：US11552361
申请日：2006-10-24
IPC分类号： G01J3/00
摘要： In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, and the transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.
摘要翻译： 在分光过程中，刺激用于产生测试谱线或样品中包含的至少一种组分的光谱的样品，并且使用透射和/或发射的电磁波来产生测试光谱线或光谱。 为了改进这种光谱过程，使得补偿了改变光谱线的形状和/或出现的某些参数的变化，已知比较材料的比较光谱线或光谱基本上在 与样品相同的参数。 将比较谱线或频谱与理想的比较谱线或频谱进行比较，以计算传递函数，并将传递函数应用于测试谱线或频谱，以便计算校正的测试谱线或频谱。
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公开(公告)号：US07145650B2
公开(公告)日：2006-12-05
申请号：US10370910
申请日：2003-02-20
摘要： In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, and the transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.
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