公开(公告)号：US06492750B2

公开(公告)日：2002-12-10

申请号：US09773737

申请日：2001-02-02

申请人： Yoshimi Kikuchi ,  Daisuke Higuchi ,  Kian Hock Yeoh

发明人： Yoshimi Kikuchi ,  Daisuke Higuchi ,  Kian Hock Yeoh

IPC分类号： G11B1920

CPC分类号： F16F15/363 ,  F16F2222/12 ,  H02K7/04 ,  Y10T74/2109 ,  Y10T74/2111

摘要： A cylindrical case provided with a chamber is coaxially attached to a rotary shaft of a motor. A plurality of balancers are movably provided within the chamber so as to cancel a rotation eccentricity of the motor. A viscous member such as high viscosity fluid is provided on at least one of the surfaces of the balancers and an inner face of the chamber.

摘要翻译： 设置有室的圆筒形壳体同轴地附接到电动机的旋转轴。 多个平衡器可移动地设置在腔室内，以抵消电动机的旋转偏心。 在平衡器的至少一个表面和室的内表面上设置诸如高粘度流体的粘性构件。

公开(公告)号：US06664670B2

公开(公告)日：2003-12-16

申请号：US09798728

申请日：2001-03-02

申请人： Yoshimi Kikuchi ,  Daisuke Higuchi

发明人： Yoshimi Kikuchi ,  Daisuke Higuchi

IPC分类号： H02K524

CPC分类号： H02K7/04 ,  H02K21/222 ,  Y10T464/50

摘要： An automatic balancing apparatus may be mounted in a rotator body such as a spindle motor having a rotary shaft defining an axis of rotation. The automatic balancing apparatus has a plurality of balancing members rotatable about the axis of rotation in a non-contact manner. Each of the balancing members has a balance action section spaced a distance in a radial direction from the axis of rotation and a connection section that rotatably couples the balance action section to the axis of rotation. The balance action section has a mass that effects a balance correction action to cancel a rotational unbalance of the rotator body when the rotator body rotates at a rotational speed that exceeds a resonant rotational speed CR thereof. The connection section has a mass that substantially does not affect the balance correction action compared to the balance action section.

公开(公告)号：US06479912B2

公开(公告)日：2002-11-12

申请号：US09764964

申请日：2001-01-17

申请人： Yoshimi Kikuchi ,  Daisuke Higuchi ,  Yeoh Kian Hock

发明人： Yoshimi Kikuchi ,  Daisuke Higuchi ,  Yeoh Kian Hock

IPC分类号： G11R1920

CPC分类号： G11B33/08 ,  F16F15/363 ,  G11B25/043

摘要： An automatic balancing apparatus which includes a support shaft which is rotated by a drive; a case body which is fixed to the support shaft and which rotates together with the support shaft; a ring magnet which is placed at the inside corner, in the radial direction, of an inner space of the case body; and a balancing member. The balancing member further includes a plurality of magnetic bodies which are movable in the inner space of the case body and which can be drawn up by the magnetic field of the ring magnet. When the rotational frequency of a rotor, which rotates together with the case body, exceeds the resonant frequency, the automatic balancing apparatus reduces vibrations of the rotor; and the outer circumference of the ring magnet has a flat portion.

摘要翻译： 一种自动平衡装置，包括由驱动器旋转的支撑轴; 壳体，其固定在所述支撑轴上并与所述支撑轴一起旋转; 环形磁体，其设置在壳体的内部空间的径向的内侧角部; 和平衡构件。 平衡构件还包括多个磁体，其可在壳体的内部空间中移动并且可以由环形磁体的磁场拉伸。 当与壳体一起旋转的转子的旋转频率超过谐振频率时，自动平衡装置减小转子的振动; 并且环形磁铁的外周具有平坦部分。

公开(公告)号：US07972829B2

公开(公告)日：2011-07-05

申请号：US12714151

申请日：2010-02-26

申请人： Yoshimi Kikuchi ,  Masayo Date ,  Yukiko Umezawa ,  Keiichi Yokoyama ,  Hiroshi Matsui

发明人： Yoshimi Kikuchi ,  Masayo Date ,  Yukiko Umezawa ,  Keiichi Yokoyama ,  Hiroshi Matsui

IPC分类号： C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04 ,  A61K38/00 ,  C07H21/04 ,  C07H21/02 ,  C07K1/00 ,  C12Q1/68 ,  C12Q1/00

CPC分类号： C12N9/1044 ,  C07K2319/02 ,  C07K2319/036 ,  C12N9/52 ,  C12N15/625 ,  C12Y203/02013

摘要： The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium.According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).An intended foreign protein, in particular, transglutaminase, is produced by using an expression construct wherein the gene sequence of the intended foreign protein containing the pro-structure part, in particular, pro-transglutaminase gene sequence, is ligated to the downstream of a sequence encoding the signal peptide region from a coryneform bacterium, introducing this expressional genetic construct into a coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the extracellularly released protein with a protease, etc. to cleave and eliminate the pro-part.

摘要翻译： 本发明涉及通过棒状细菌分泌外源蛋白质，特别是转谷氨酰胺酶的方法。 根据本发明，提供了通过制备棒状细菌以产生工业上有用的外源蛋白质，特别是转谷氨酰胺酶并有效地将细胞外释放产物（即，通过转运谷氨酰胺酶）分泌外源蛋白质，特别是转谷氨酰胺酶的方法， 分泌生产）。 通过使用表达构建体产生预期的外源蛋白质，特别是转谷氨酰胺酶，其中含有前结构部分，特别是原转谷氨酰胺酶基因序列的预期外源蛋白的基因序列连接到序列的下游 编码来自棒状细菌的信号肽区域，将该表达遗传构建体引入棒状杆菌型细菌，培养由此转化的棒状杆菌型细菌，用蛋白酶等处理细胞外释放的蛋白质以切割和消除前体部分。

公开(公告)号：US20110137007A1

公开(公告)日：2011-06-09

申请号：US12983500

申请日：2011-01-03

申请人： Mototaka Suzuki ,  Keiichi Yokoyama ,  Yoshimi Kikuchi

发明人： Mototaka Suzuki ,  Keiichi Yokoyama ,  Yoshimi Kikuchi

IPC分类号： C08G73/22 ,  C12P13/00

CPC分类号： C12P13/008 ,  C08G73/22 ,  C12N9/16 ,  C12N9/88

摘要： Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.

摘要翻译： 提供了通过培养具有编码不经反馈抑制的突变型天冬氨酸激酶的基因的棒状细菌，并且用含有编码的DNA的重组载体转化的有效产生3-氨基-4-羟基苯甲酸型化合物的方法 具有从磷酸二羟丙酮和天冬氨酸半醛形成3-氨基-4-羟基苯甲酸的活性的蛋白质。

公开(公告)号：US20100297729A1

公开(公告)日：2010-11-25

申请号：US12714151

申请日：2010-02-26

申请人： Yoshimi Kikuchi ,  Masayo Date ,  Yukiko Umezawa ,  Keiichi Yokoyama ,  Hiroshi Matsui

发明人： Yoshimi Kikuchi ,  Masayo Date ,  Yukiko Umezawa ,  Keiichi Yokoyama ,  Hiroshi Matsui

IPC分类号： C12N9/48 ,  C07H21/04

CPC分类号： C12N9/1044 ,  C07K2319/02 ,  C07K2319/036 ,  C12N9/52 ,  C12N15/625 ,  C12Y203/02013

摘要： The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium.According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).An intended foreign protein, in particular, transglutaminase, is produced by using an expression construct wherein the gene sequence of the intended foreign protein containing the pro-structure part, in particular, pro-transglutaminase gene sequence, is ligated to the downstream of a sequence encoding the signal peptide region from a coryneform bacterium, introducing this expressional genetic construct into a coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the extracellularly released protein with a protease, etc. to cleave and eliminate the pro-part.

摘要翻译： 本发明涉及通过棒状细菌分泌外源蛋白质，特别是转谷氨酰胺酶的方法。 根据本发明，提供了通过制备棒状细菌以产生工业上有用的外源蛋白质，特别是转谷氨酰胺酶并有效地释放产物的方法（即，通过使细胞外有效释放外源蛋白质，特别是转谷氨酰胺酶） 分泌生产）。 通过使用表达构建体产生预期的外源蛋白质，特别是转谷氨酰胺酶，其中含有前结构部分，特别是原转谷氨酰胺酶基因序列的预期外源蛋白的基因序列连接到序列的下游 编码来自棒状细菌的信号肽区域，将该表达遗传构建体引入棒状杆菌型细菌，培养由此转化的棒状杆菌型细菌，用蛋白酶等处理细胞外释放的蛋白质以切割和消除前体部分。

公开(公告)号：US07723081B1

公开(公告)日：2010-05-25

申请号：US10149450

申请日：2000-01-21

申请人： Kazuo Nakanishi ,  Yoshimi Kikuchi ,  Junichiro Kojima ,  Tomoko Suzuki ,  Yasushi Nishimura ,  Hiroyuki Kojima

发明人： Kazuo Nakanishi ,  Yoshimi Kikuchi ,  Junichiro Kojima ,  Tomoko Suzuki ,  Yasushi Nishimura ,  Hiroyuki Kojima

IPC分类号： C12P13/08

CPC分类号： C12P13/08 ,  C12N15/52

摘要： An Escherichia bacterium (1) which harbors dihydrodipicolinate synthase of which feedback inhibition by L-lysine is desensitized and aspartokinase of which feedback inhibition by L-lysine is desensitized, (2) in which intracellular activity of dihydrodipicolinate reductase is enhanced, and (3) in which a diaminopimelate dehydrogenase gene is introduced or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase are enhanced, wherein intracellular activity of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase is enhanced, is cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.

摘要翻译： 具有L-赖氨酸的反馈抑制脱敏的二氢吡啶并吡啶合酶的大肠杆菌细菌（1），L-赖氨酸的反馈抑制的天冬氨酸脱敏，（2）二氢吡啶二羧酸还原酶的细胞内活性增强，（3） 其中引入二氨基庚二酸脱氢酶基因或增加四氢二吡啶甲酰基琥珀酰基酶和琥珀酰二氨基庚二酸脱酰基酶的细胞内活性，其中天冬氨酸半醛脱氢酶或磷酸烯醇丙酮酸羧化酶的细胞内活性增强，在合适的培养基中培养以在培养物中产生和积累L-赖氨酸 ，并从培养物中收集L-赖氨酸。

公开(公告)号：US07704707B2

公开(公告)日：2010-04-27

申请号：US11218780

申请日：2005-09-06

申请人： Yukiko Umezawa ,  Keiichi Yokoyama ,  Yoshimi Kikuchi ,  Masayo Date ,  Norimasa Onishi

发明人： Yukiko Umezawa ,  Keiichi Yokoyama ,  Yoshimi Kikuchi ,  Masayo Date ,  Norimasa Onishi

IPC分类号： C12P21/06 ,  C12N9/48

CPC分类号： C12N9/1044 ,  C12N9/6489

摘要： The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.

摘要翻译： 本发明提供来自放线菌的中性金属蛋白酶，其选择性地切割微生物转谷氨酰胺酶的前体结构部分和编码所述中性金属蛋白酶的基因。 具有前体结构部分切割的活性微生物转谷氨酰胺酶可以通过培养从引入放线菌中产生中性金属蛋白酶的本发明的编码中性金属蛋白酶的基因的微生物获得，其中通过从放线菌产生中性金属蛋白酶， 微生物抗转谷氨酰胺酶。

公开(公告)号：US20070184525A1

公开(公告)日：2007-08-09

申请号：US11550972

申请日：2006-10-19

申请人： Masayo Date ,  Yoshimi Kikuchi ,  Hiroshi Itaya ,  Nami Nakamura

发明人： Masayo Date ,  Yoshimi Kikuchi ,  Hiroshi Itaya ,  Nami Nakamura

IPC分类号： C12P21/06 ,  C12N9/06 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12P21/02 ,  C12N15/77

摘要： The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5′-end to 3′-end, and secretory producing the heterologous protein by coryneform bacteria.

摘要翻译： 本发明提供了一种在棒状细菌中有效生产工业上有用的蛋白质的方法，更具体地，涉及使用常规的蛋白质分泌途径有效制备难以分泌的蛋白质的方法。 特别地，本发明提供了一种有效生产异源蛋白的方法，包括培养含有在棒状细菌中起作用的启动子序列的遗传构建的棒状细菌，编码Tat系统依赖性信号肽区的核酸序列和核酸 从5'端到3'端的方向编码异源蛋白的序列，以及通过棒状细菌产生异源蛋白的分泌。

公开(公告)号：US5958128A

公开(公告)日：1999-09-28

申请号：US945266

申请日：1997-10-24

申请人： Yoshimi Kikuchi ,  Hitoshi Sugamo

发明人： Yoshimi Kikuchi ,  Hitoshi Sugamo

IPC分类号： C09B35/10 ,  C09B41/00 ,  C09B27/00

CPC分类号： C09B35/105 ,  C09B41/006

摘要： In the method of manufacturing a disazo pigment according to the present invention, a superior coupling reaction rate during the coupling reaction process makes it possible to manufacture a disazo pigment having superior transparency, brightness, color strength and gloss for the case in which a color strength agent is used. In this method, a coupling reaction is carried out while continuously feeding a coupler aqueous solution comprising a coupler component having the general formula (I) shown below and a tetrazo aqueous solution comprising a benzidine derivative tetrazo component at a molar ratio of 2:0.8.about.0.99 into an acidic aqueous solution held in a batch type mixing vessel. ##STR1## (where each of X, Y, Z independently represents H, CH.sub.3, OCH.sub.3, Cl)

摘要翻译： PCT No.PCT / JP97 / 00547 Sec。 371 1997年10月24日第 102（e）1997年10月24日PCT PCT 1997年2月26日提交PCT公布。 公开号WO97 / 31067 日本公开日1997年8月28日在本发明的双偶氮颜料的制造方法中，在偶联反应工序中优异的偶联反应速度使得可以制造具有优异透明性，亮度，着色强度和光泽度的双偶氮颜料 其中使用着色剂。 在该方法中，连续进料包含下述通式（I）所示的成色剂组分的偶合剂水溶液和包含联苯胺衍生物双偶氮组分的双偶氮水溶液，摩尔比为2:0.8。 0.99进入分批式混合容器中的酸性水溶液中。 （其中X，Y，Z各自独立地表示H，CH 3，OCH 3，Cl）