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公开(公告)号：US07304315B2
公开(公告)日：2007-12-04
申请号：US11404248
申请日：2006-04-13
申请人： Yoshinori Iketaki , Masaaki Fujii , Takeshi Watanabe , Takashige Omatsu , Kimihisa Yamamoto , Toshio Suzuki
发明人： Yoshinori Iketaki , Masaaki Fujii , Takeshi Watanabe , Takashige Omatsu , Kimihisa Yamamoto , Toshio Suzuki
CPC分类号： G01N21/6458 , G01N21/645 , G01N2021/6419
摘要： A three-dimensional analyzing device includes a first beam source for generating a first beam, a second beam source for generating a second beam, an optical system for spatially overlapping the first and second beams at least partly and irradiating the beams onto a specimen to three-dimensionally confine a photoactive region in a specimen, and a photo acceptance element for accepting a response light emitted from the photoactive region. Preferably, the device further includes an operation unit for calculating a correlation function of a response light in the time domain based on the output of the photo acceptance element to analyze a desired physical value of the specimen.
摘要翻译： 三维分析装置包括用于产生第一光束的第一光束源，用于产生第二光束的第二光束源，至少部分地将第一和第二光束空间重叠的光学系统，并将光束照射到样品上至三 视觉上将光活性区域限制在样本中，以及用于接受从光活性区域发射的响应光的光接收元件。 优选地，所述装置还包括操作单元，用于基于所述光接收元件的输出来计算所述时域中的响应光的相关函数，以分析所述样本的期望物理值。
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公开(公告)号：US20060290924A1
公开(公告)日：2006-12-28
申请号：US11404248
申请日：2006-04-13
申请人： Yoshinori Iketaki , Masaaki Fujii , Takeshi Watanabe , Takashige Omatsu , Kimihisa Yamamoto , Toshio Suzuki
发明人： Yoshinori Iketaki , Masaaki Fujii , Takeshi Watanabe , Takashige Omatsu , Kimihisa Yamamoto , Toshio Suzuki
IPC分类号： G01J3/00
CPC分类号： G01N21/6458 , G01N21/645 , G01N2021/6419
摘要： A three-dimensional analyzing device includes a first beam source for generating a first beam, a second beam source for generating a second beam, an optical system for spatially overlapping the first and second beams at least partly and irradiating the beams onto a specimen to three-dimensionally confine a photoactive region in a specimen, and a photo acceptance element for accepting a response light emitted from the photoactive region. Preferably, the device further includes an operation unit for calculating a correlation function of a response light in the time domain based on the output of the photo acceptance element to analyze a desired physical value of the specimen.
摘要翻译： 三维分析装置包括用于产生第一光束的第一光束源，用于产生第二光束的第二光束源，用于至少部分地将第一和第二光束空间重叠的光学系统，并将光束照射到样品上至三 视觉上将光活性区域限制在样本中，以及用于接受从光活性区域发射的响应光的光接收元件。 优选地，所述装置还包括操作单元，用于基于所述光接收元件的输出来计算所述时域中的响应光的相关函数，以分析所述样本的期望物理值。
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公开(公告)号：US06859313B2
公开(公告)日：2005-02-22
申请号：US10103190
申请日：2002-03-21
CPC分类号： G02B21/06
摘要： A microscope includes a first light source to emit a first light to excite a molecule of a sample to a higher energy level vibration state which belongs to a lowest energy level electron state from the ground state, a second light source to emit a second light source to excite the molecule to a higher energy level quantum state from the higher energy vibration state, an optical system to overlap the first light and the second light partially on the sample, and an optical detector to detect a given fluorescence from the irradiated region of the first light and the second light on the sample.
摘要翻译： 显微镜包括：第一光源，用于发射第一光以将样品的分子激发到属于基本状态的最低能量级电子态的较高能级振动状态，第二光源发射第二光源 从更高的能量振动状态激发分子到更高的能量级量子态，光学系统将部分地与样品上的第一光和第二光重叠，以及光学检测器，用于检测来自辐射源的照射区域的给定荧光 样品上的第一个光和第二个光。
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公开(公告)号：US06667830B1
公开(公告)日：2003-12-23
申请号：US09445389
申请日：2000-03-17
IPC分类号： G02B2100
CPC分类号： G01N21/6458 , G01N21/636 , G01N21/64 , G01N21/6428 , G01N21/6445 , G02B21/0032 , G02B21/0056 , G02B21/0072 , G02B21/0076 , G02B21/16 , G21K7/00
摘要： A microscope system comprising an adjusted specimen and a microscope body, wherein the adjusted specimen is dyed with molecule which has three electronic states including at least a ground state and in which an excited wavelength band from the first electron excited state to the second electron excited state overlaps a fluorescent wavelength band upon deexcitation through a fluorescence process from the first electron excited state to a vibrational level in the ground state. There is provided a novel microscope system which is enabled to condense an erase light for exciting a molecule in the first electron excited state to the second electron excited state in an excellent beam profile by using a simple, compact optical system and which has high stability and operability and an excellent super-resolution.
摘要翻译： 一种显微镜系统，包括经调整的样本和显微镜主体，其中所述经调整的样本用分子染色，所述分子具有至少包括基态的三种电子状态，并且其中从第一电子激发态到第二电子激发态的激发波长带 通过从第一电子激发状态到基态的振动电平的荧光过程在激发下与荧光波长带重叠。 提供了一种新颖的显微镜系统，其能够通过使用简单紧凑的光学系统来将用于将第一电子激发态的分子激发到第二电子激发态的擦除光以优异的光束轮廓冷凝，并且具有高稳定性和 可操作性和出色的超分辨率。
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公开(公告)号：US07095556B2
公开(公告)日：2006-08-22
申请号：US10771729
申请日：2004-02-04
CPC分类号： G02B26/08
摘要： A microscope capable of being shaped into a beam with complete hollow shape by removing the disorder of the wavefront to the erase light, particularly, and capable of improving the spatial resolution by inducing a super-resolution near the limit, is provided. In the microscope, wherein a first light to excite a molecule from a ground-state to first electron excited state or a second light to excite the molecule from the first electron excited state to the second electron excited state with higher energy level, for a sample 56 including the molecule with three electronic states including at least a ground-state, are spatial phase-modulated into the prescribed beam shape, and parts of these first light and the second light are overlapped and focused to detect luminescence from the sample 56, a wavefront compensation means 61 is provided in the optical path of the first light and/or in the optical path of the second light, and the wavefront aberration caused in the first light and/or in the second light, is removed by the wavefront compensation means 61.
摘要翻译： 提供了能够通过将擦除光的波阵面除去特别是并且能够通过在接近极限附近引起超分辨率来改善空间分辨率而将具有完全中空形状的光束成形为显微镜的显微镜。 在显微镜中，其中将分子从基态激发到第一电子激发态的第一光或第二光以将分子从第一电子激发态激发至具有较高能级的第二电子激发态，对于样品 包括具有至少包括基态的三种电子状态的分子被空间相位调制成规定的波束形状，并且这些第一光和第二光的一部分被重叠和聚焦以检测来自样品56的发光， 波前补偿装置61设置在第一光的光路和/或第二光的光路中，并且在第一光和/或第二光中引起的波前像差被波前补偿装置除去 61。
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公开(公告)号：US5835262A
公开(公告)日：1998-11-10
申请号：US580196
申请日：1995-12-28
申请人： Yoshinori Iketaki , Masaaki Fujii
发明人： Yoshinori Iketaki , Masaaki Fujii
CPC分类号： G02B21/06
摘要： A high-accuracy multi-wavelength optical microscope for providing a satisfactory contrast of image and much information about a sample. The microscope includes a plurality of light sources, a wavelength varietor independently varying the wavelength of the individual light sources, and polarization plane rotators on the optical path for each light source.
摘要翻译： 一种高精度多波长光学显微镜，用于提供令人满意的图像对比度和关于样品的大量信息。 显微镜包括多个光源，独立地改变各个光源的波长的波长变换器和用于每个光源的光路上的偏振面旋转器。
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公开(公告)号：US20070291353A1
公开(公告)日：2007-12-20
申请号：US11659525
申请日：2005-07-25
CPC分类号： G01N21/6458 , G01N2021/6419 , G01N2021/6439 , G02B21/002 , G02B21/16 , G02B27/58
摘要： [Task] To provide a super-resolution microscope whereby the light source of pump light and erase light can be selected easily and a super-resolution can be reliably achieved through a simple and inexpensive arrangement. [Solution of the Task] A super-resolution microscope includes an optical system (3, 4, 9) for combining a part of a first coherent light from a first light source (2) and a part of a second coherent light from a second light source (1) and focusing the coherent lights onto a sample (10), scanning means (6, 7) for scanning the coherent lights, and detecting means (16) for detecting an optical response signal from the sample (10). The microscope is configured so as to satisfy the following conditions: σ01Ipτ≦1, and 0.65(λe/λp)≦τσdipIe where λp is the wavelength of the first coherent light, λe is the wavelength of the second coherent light, τ is the excited lifetime in which the molecule is excited by the first coherent light from the ground state to the first electron-excited state, Ip is the maximum photon flux on the sample surface of the first coherent light, Ie is the maximum photon flux on the sample surface of the second coherent light, σ01, is the absorption cross-sectional area when the molecule is exited from the ground state to the first electron-excited state, and σdip is the fluorescence suppression cross-sectional area.
摘要翻译： [任务]提供一种能够容易地选择泵浦光和擦除光的光源的超分辨率显微镜，并且通过简单且廉价的布置可以可靠地实现超分辨率。 [任务的解决方案]超分辨率显微镜包括用于组合来自第一光源（2）的第一相干光的一部分和来自第二光源的第二相干光的一部分的光学系统（3,4,9） 光源（1），并将相干光聚焦到样品（10）上，用于扫描相干光的扫描装置（6,7）以及用于检测来自样品（10）的光响应信号的检测装置（16）。 显微镜被配置为满足以下条件：<？in-line-formula description =“In-line Formulas”end =“lead”？> sigma Iptau <= 1， ？in-line-formula description =“In-line Formulas”end =“tail”？> <？in-line-formula description =“In-line formula”end =“lead”？> 0.65（lambdae / lambdap） = tausigma Ie <？in-line-formula description =“In-line Formulas”end =“tail”？>其中lambdap是第一个相干光的波长，lambdae是 第二相干光，tau是分子被从基态到第一电子激发态的第一相干光激发的激发寿命，Ip是第一相干光的样品表面上的最大光子通量，Ie是 第二相干光的样品表面上的最大光子通量σσ是当分子从基态退出到第一电子激发态时的吸收横截面积，而西格玛 浸渍 是荧光抑制截面积。
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公开(公告)号：US07551350B2
公开(公告)日：2009-06-23
申请号：US11659525
申请日：2005-07-25
CPC分类号： G01N21/6458 , G01N2021/6419 , G01N2021/6439 , G02B21/002 , G02B21/16 , G02B27/58
摘要： A super-resolution microscope includes an optical system for combining a part of a first coherent light from a first light source and a part of a second coherent light from a second light source and focusing the coherent lights onto a sample, scanning , unit for scanning the coherent lights, and a detecting unit for detecting an optical response signal from the sample. The microscope is configured so as to satisfy the following conditions: σ01Ipτ≦1, and 0.65(λe/λp)≦τσdipIe.
摘要翻译： 超分辨率显微镜包括用于组合来自第一光源的第一相干光的一部分和来自第二光源的第二相干光的一部分的光学系统，并将相干光聚焦到样本上，扫描单元进行扫描 相干光，以及用于检测来自样品的光响应信号的检测单元。 显微镜配置为满足以下条件：<？in-line-formula description =“In-line Formulas”end =“lead”？> sigma01Iptau <= 1，<？in-line-formula description =“ 在线公式“end =”tail“？> <？in-line-formula description =”In-line Formulas“end =”lead“？> 0.65（lambdae / lambdap）<= tausigmadipIe。 <？in-line-formula description =“In-line Formulas”end =“tail”？>
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公开(公告)号：US08947658B2
公开(公告)日：2015-02-03
申请号：US13610563
申请日：2012-09-11
申请人： Yoshinori Iketaki , Hideaki Kano
发明人： Yoshinori Iketaki , Hideaki Kano
CPC分类号： G01N21/65 , G01J3/10 , G01J3/44 , G01N2021/653 , G02B21/0004 , G02B21/082 , G02B21/14 , G02B27/425 , G02B27/44
摘要： Provided is a nonlinear optical microscope capable of improving the spatial resolution. The nonlinear optical microscope includes: an illuminating part for irradiating, through an objective lens, a sample with at least two colors of illumination light beams spatially and temporally overlapping each other; and a detecting part for detecting signal light generated from the sample due to nonlinear optical effect, the signal light resulting from the irradiation of the sample with the at least two colors of illumination light beams, in which the illuminating part irradiates the sample with the two colors of illumination light beams in which at least one of the illumination light beams has a wavefront distribution different from a wavefront distribution of the other one of the illumination light beams.
摘要翻译： 提供了能够提高空间分辨率的非线性光学显微镜。 非线性光学显微镜包括：照明部，其通过物镜照射具有空间和时间上彼此重叠的至少两种照明光束的样品; 以及检测部，其用于检测由于非线性光学效应而从样本产生的信号光，所述信号光由具有所述至少两种颜色的照明光束的所述样本的照射而产生，所述照明部分照射所述样本与所述两个 其中至少一个照明光束具有与另一个照明光束的波前分布不同的波前分布的照明光束的颜色。
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公开(公告)号：US06184535B2
公开(公告)日：2001-02-06
申请号：US09156775
申请日：1998-09-18
申请人： Shingo Kashima , Yoshinori Iketaki
发明人： Shingo Kashima , Yoshinori Iketaki
IPC分类号： G01N2164
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6419 , G02B21/002 , G02B21/16
摘要： In a method of observing a specimen through a fluorescence microscope, molecules that could cause double-resonance absorption process in an appropriate manner are used to dye the specimen, and the specimen is irradiated with a plurality of light beams of different wavelengths in a good timing, so that a microscopic image with high spatial resolution is obtained. In the microscope to be used in the method of observation, the specimen is irradiated with two light beams of different wavelength bands via an aperture with an annular-zonal structure and an optical system that has different in-focus positions for these two wavelength bands, so that one of the two light beam is focused on a surface of the specimen and the other light beam is defocused thereon. Consequently, the microscope achieves improvement of the spatial resolution as to overcome the diffraction limit determined by the wavelengths in use.
摘要翻译： 在通过荧光显微镜观察样品的方法中，使用可以以适当的方式引起双共振吸收过程的分子染色样品，并且以良好的时间用多个不同波长的光束照射样品 ，从而获得具有高空间分辨率的显微图像。在用于观察方法的显微镜中，通过具有环形区域结构和光学系统的孔径，用两个不同波长带的光束照射样品 对于这两个波长带具有不同的对焦位置，使得两个光束中的一个被聚焦在样本的表面上，并且另一个光束在其上散焦。 因此，显微镜实现空间分辨率的提高，以克服由使用的波长确定的衍射极限。
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