Method for L-threonine production
    1.
    发明授权
    Method for L-threonine production 有权
    L-苏氨酸生产方法

    公开(公告)号:US07378267B1

    公开(公告)日:2008-05-27

    申请号:US10508728

    申请日:2002-05-16

    IPC分类号: C12N1/12 C12P13/04

    CPC分类号: C12P13/08

    摘要: A method for producing L-threonine using a microorganism is provided. In the method, the threonine dehydratase (tdc) gene existing in the genomic DNA of the microorganism is partially deactivated using a recombination technique. For a microorganism strain with enhanced activity of threonine operon-containing enzymes and the phosphoenolpyruvate carboxylase (ppc) gene, the tdc gene engaged in one of the four threonine metabolic pathways is specifically deactivated, thereby markedly increasing the yield of L-threonine.

    摘要翻译: 提供了使用微生物生产L-苏氨酸的方法。 在该方法中,存在于微生物的基因组DNA中的苏氨酸脱水酶(tdc)基因使用重组技术部分失活。 对于具有苏氨酸操纵子的酶和磷酸烯醇丙酮酸羧化酶(ppc)基因的活性增强的微生物菌株,特异性地去除了四种苏氨酸代谢途径之一的tdc基因,从而显着增加了L-苏氨酸的产量。

    tdcBC/pckA gene-inactivated microorganism and method of producing L-threonine using the same
    2.
    发明授权
    tdcBC/pckA gene-inactivated microorganism and method of producing L-threonine using the same 有权
    tdcBC / pckA基因灭活的微生物和使用其生产L-苏氨酸的方法

    公开(公告)号:US07485450B2

    公开(公告)日:2009-02-03

    申请号:US10817044

    申请日:2004-04-02

    IPC分类号: C12N1/20

    CPC分类号: C12P13/08 C12N9/88

    摘要: The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.

    摘要翻译: 本发明提供了包含灭活的染色体tdcBC基因和灭活的染色体pckA基因的微生物,其显着提高了L-苏氨酸的生产率。 另外,本发明提供使用该微生物生产L-苏氨酸的方法。 通过重组技术将抗生素抗性基因并入包含失活的L-苏氨酸降解相关操纵子基因tdcBC的细菌菌株的染色体上的pckA基因中来制备微生物。 该微生物具有防止由于tdcBC操纵子基因失活引起的L-苏氨酸的降解和细胞内流入的作用,并且包括用于L-苏氨酸生物合成的更多活化途径。 因此,即使在高浓度葡萄糖的存在下,微生物也能够以高水平和高产率生产L-苏氨酸,因此可用于批量生产L-苏氨酸。

    Method for producing L-threonine
    3.
    发明授权
    Method for producing L-threonine 有权
    生产L-苏氨酸的方法

    公开(公告)号:US07074602B2

    公开(公告)日:2006-07-11

    申请号:US10916804

    申请日:2004-08-12

    CPC分类号: C12P13/08

    摘要: An L-threonine-producing Escherichia coli strain and a method for producing the same are provided. The Escherchia coli strain contains chromosomal DNA with inactivated metJ gene. Therefore, expression repression of threonine biosynthesis genes by a metJ gene product is prevented, thereby producing a high concentration of threonine. Further, a high concentration of L-threonine can be produced in high yield using the method.

    摘要翻译: 提供了产生L-苏氨酸的大肠杆菌菌株及其制备方法。 大肠埃希氏菌菌株含有具有灭活的metJ基因的染色体DNA。 因此,通过metJ基因产物抑制苏氨酸生物合成基因的表达抑制,从而产生高浓度的苏氨酸。 此外,可以使用该方法以高产率制备高浓度的L-苏氨酸。

    Optical sensor
    9.
    发明授权
    Optical sensor 有权
    光学传感器

    公开(公告)号:US08598587B2

    公开(公告)日:2013-12-03

    申请号:US13209188

    申请日:2011-08-12

    IPC分类号: H01L31/0376

    摘要: An optical sensor preventing damage to a semiconductor layer, and preventing a disconnection and a short circuit of a source electrode and a drain electrode, and a manufacturing method of the optical sensor is provided. The optical sensor includes: a substrate; an infrared ray sensing thin film transistor including a first semiconductor layer disposed on the substrate; a visible ray sensing thin film transistor including a second semiconductor layer disposed on the substrate; a switching thin film transistor including a third semiconductor layer disposed on the substrate; and a semiconductor passivation layer enclosing an upper surface and a side surface of an end portion of at least one of the first semiconductor layer, the second semiconductor layer, and the third semiconductor layer.

    摘要翻译: 提供了防止半导体层损坏并防止源电极和漏极的断开和短路的光学传感器以及光学传感器的制造方法。 光学传感器包括:基板; 包括设置在所述基板上的第一半导体层的红外线感测薄膜晶体管; 包括设置在所述基板上的第二半导体层的可见光线感测薄膜晶体管; 开关薄膜晶体管,其包括设置在所述基板上的第三半导体层; 以及半导体钝化层,其包围第一半导体层,第二半导体层和第三半导体层中的至少一个的端部的上表面和侧面。