公开(公告)号：US4372745A

公开(公告)日：1983-02-08

申请号：US233057

申请日：1981-02-10

申请人： Richard M. Mandle ,  Yuan N. Wong

发明人： Richard M. Mandle ,  Yuan N. Wong

IPC分类号： G01N33/58 ,  G01N33/54 ,  G01N33/52

CPC分类号： G01N33/582 ,  G01N33/586 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/826

摘要： A system for the detection of a biological analyte of interest is disclosed which comprises a microencapsulated fluorescer material which has been conjugated to an immunological specie specific to the biological analyte of interest, a means of disrupting the capsule containing the fluorescer and an energy source other than electro-magnetic radiation which is capable of activating the fluorescer. A method for the qualitative and/or quantitative detection of a biological of interest is disclosed, which comprises: (a) labeling an immunological specie specific to the analyte of interest with a microencapsulated fluorescer material which is biologically compatible with such specie;(b) contacting the microencapsulated fluorescer labeled specie and the biological of interest to form a microencapsulated fluorescer labeled specie/biological complex;(c) separating the microencapsulated fluorescer labeled specie/biological complex;(d) distrupting the capsule containing the fluorescer label thus freeing it to solution;(e) contacting the freed fluorescer with an energy source other than electro-magnetic radiation which is capable of activating the fluorescer label; and(f) determining the presence of and/or measuring the quantity of chemiluminescent light emitted.

摘要翻译： 公开了一种用于检测感兴趣的生物分析物的系统，其包含已经与感兴趣的生物分析物特异性的免疫学特异性结合的微囊化荧光剂材料，破坏包含荧光剂的胶囊和除了 能够激活荧光剂的电磁辐射。 公开了用于定性和/或定量检测感兴趣的生物学的方法，其包括：（a）用与这种物质生物相容的微囊化荧光剂材料标记对目标分析物特异性的免疫学物种; （b）使微囊化的荧光物质标记的物质和感兴趣的生物学接触以形成微囊化的荧光剂标记的物种/生物复合物; （c）分离微囊化的荧光剂标记的物种/生物复合物; （d）破坏含有荧光标签的胶囊，从而释放出溶液; （e）使除去的荧光剂与能够激活荧光标记物的电磁辐射以外的能量源接触; 和（f）确定发射的化学发光光的量的存在和/或测量。

公开(公告)号：US5904848A

公开(公告)日：1999-05-18

申请号：US604440

申请日：1996-02-21

申请人： Yuan N. Wong ,  Richard Chen

发明人： Yuan N. Wong ,  Richard Chen

IPC分类号： B01D15/08 ,  B01D15/36 ,  B01D15/38 ,  B01D67/00 ,  B01D69/14 ,  B01D71/04 ,  B01D71/36 ,  B01J20/28 ,  B01J20/32 ,  B01J41/20 ,  C07B61/00 ,  C07H21/00 ,  G01N33/552 ,  B01D39/00 ,  B01D39/14 ,  B01D71/26

CPC分类号： B01D67/0093 ,  B01D69/141 ,  B01D71/04 ,  B01D71/36 ,  B01J20/28004 ,  B01J20/28026 ,  B01J20/28033 ,  B01J20/28085 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3255 ,  B01J20/3274 ,  B01J41/20 ,  C07H21/00 ,  G01N33/552 ,  B01D15/361 ,  B01D15/3804 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00641 ,  C07B2200/11 ,  C40B40/00

摘要： Particulate inorganic pore material, e.g., controlled pore glass (CPG) embedded porous synthetic resin membrane is prepared by mixing inorganic pore material and an aqueous resin, preferably polytetrafluoroethylene (PTFE), aqueous dispersion to form a paste-like mass, heating the mass at a temperature between 50 to 70.degree. C., and forming the mass into a sheet by calendering. The sheet is then sintered to produce a rigid porous sheet. The membrane may be functionalized, as by silanization. The membrane is useful for the same purposes as controlled pore glass or functionalized controlled pore glass.

摘要翻译： 通过混合无机孔隙材料和水性树脂，优选聚四氟乙烯（PTFE）水分散体来制备颗粒状无机孔隙材料，例如可控孔隙玻璃（CPG）嵌入的多孔合成树脂膜，以形成糊状物质， 温度在50至70℃之间，并通过压延将组合物形成片材。 然后将片材烧结以产生刚性多孔片材。 膜可以通过硅烷化官能化。 该膜可用于与受控孔玻璃或官能化控制孔玻璃相同的目的。

公开(公告)号：US06261497B1

公开(公告)日：2001-07-17

申请号：US09305219

申请日：1999-05-04

申请人： Yuan N. Wong ,  Richard Chen

发明人： Yuan N. Wong ,  Richard Chen

IPC分类号： B29C6704

CPC分类号： B01D67/0093 ,  B01D15/361 ,  B01D15/3804 ,  B01D69/141 ,  B01D71/04 ,  B01D71/36 ,  B01J20/28004 ,  B01J20/28026 ,  B01J20/28033 ,  B01J20/28085 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3255 ,  B01J20/3274 ,  B01J41/20 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00641 ,  C07B2200/11 ,  C07H21/00 ,  C40B40/00 ,  G01N33/552

摘要： Particulate inorganic pore material, e.g., controlled pore glass (CPG) embedded porous synthetic resin membrane is prepared by mixing inorganic pore material and an aqueous resin, preferably polytetrafluoroethylene (PTFE), aqueous dispersion to form a paste-like mass, heating the mass at a temperature between 50 to 70° C., and forming the mass into a sheet by calendering. The sheet is then sintered to produce a rigid porous sheet. The membrane may be functionalized, as by silanization. The membrane is useful for the same purposes as controlled pore glass or functionalized controlled pore glass.

摘要翻译： 通过混合无机孔隙材料和水性树脂，优选聚四氟乙烯（PTFE）水分散体来制备颗粒状无机孔隙材料，例如可控孔隙玻璃（CPG）嵌入的多孔合成树脂膜，以形成糊状物质， 温度在50至70℃之间，并通过压延将组合物形成片材。 然后将片材烧结以产生刚性多孔片材。 膜可以通过硅烷化官能化。 该膜可用于与受控孔玻璃或官能化控制孔玻璃相同的目的。

公开(公告)号：US5601979A

公开(公告)日：1997-02-11

申请号：US307307

申请日：1994-09-16

申请人： Yuan N. Wong

发明人： Yuan N. Wong

IPC分类号： C03C11/00 ,  C03C14/00 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/70 ,  G01N33/00

CPC分类号： C03C14/004 ,  C03C11/00 ,  C03C2214/04 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/838 ,  Y10S977/845 ,  Y10S977/895 ,  Y10S977/902 ,  Y10S977/915 ,  Y10T436/196666 ,  Y10T436/20

摘要： A functionalized, magnetic controlled pore glass support useful in solid phase DNA synthesis is described. The support acts as a universal solid phase for direct oligonucleotide synthesis. The oligonucleotide bound MPG is useful directly to isolate or purify oligonucleotides which possess a section of complementary sequences.

摘要翻译： 描述了可用于固相DNA合成的官能化的磁控孔隙玻璃载体。 支持体作为直接寡核苷酸合成的通用固相。 结合MPG的寡核苷酸可直接用于分离或纯化具有一段互补序列的寡核苷酸。

公开(公告)号：US5734020A

公开(公告)日：1998-03-31

申请号：US460130

申请日：1995-06-02

申请人： Yuan N. Wong

发明人： Yuan N. Wong

IPC分类号： C03C11/00 ,  C03C14/00 ,  C07H1/00 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C03C14/004 ,  C03C11/00 ,  C03C2214/04 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/838 ,  Y10S977/895 ,  Y10S977/896 ,  Y10S977/902 ,  Y10S977/915

摘要： Magnetic porous inorganic siliceous materials having a particle size of about 1 to about 200 microns useful as solid supports in various chromatography, immunoassays, synthesis and other separation and purification procedures is disclosed.

摘要翻译： 公开了在各种色谱，免疫测定，合成和其它分离和纯化方法中用作固体支持物的粒径约1至约200微米的磁性无机硅质材料。

公开(公告)号：US5610274A

公开(公告)日：1997-03-11

申请号：US952264

申请日：1992-09-28

申请人： Yuan N. Wong

发明人： Yuan N. Wong

IPC分类号： C03C11/00 ,  C03C14/00 ,  A61K38/02

CPC分类号： C03C14/004 ,  C03C11/00 ,  C03C2214/04 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/838 ,  Y10S977/845 ,  Y10S977/895 ,  Y10S977/902 ,  Y10S977/915 ,  Y10T436/196666 ,  Y10T436/20

摘要： Magnetic porous inorganic siliceous materials having a particle size of about 1 to about 200 microns useful as solid supports in various chromatography, immunoassays, synthesis and other separation and purification procedures as disclosed.

摘要翻译： 在各种色谱，免疫测定，合成和其它所公开的分离和纯化方法中，具有约1至约200微米粒度的磁性无机硅质材料可用作固体载体。