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公开(公告)号：US5229288A
公开(公告)日：1993-07-20
申请号：US690132
申请日：1991-04-23
申请人： Yuichi Mori , Toshiaki Takezawa , Manabu Yamazaki
发明人： Yuichi Mori , Toshiaki Takezawa , Manabu Yamazaki
IPC分类号： C12M3/00 , C12N5/06 , C12Q1/04 , G01N33/50 , G01N33/569
CPC分类号： G01N33/5014 , G01N33/56977
摘要： A cytotoxicity test method is provided which measures the effect of test substances or physical stimulation on cells. The method is both simple and sensitive and is useful for a wide variety of substances and stimulations.
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公开(公告)号：US11198264B2
公开(公告)日：2021-12-14
申请号：US14326817
申请日：2014-07-09
申请人： NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES , Toshiaki Takezawa , KANTO KAGAKU KABUSHIKI KAISHA
摘要： A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.
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3.发明申请Carrier for co-culturing a fertilized ovum of an animal and method for culturing a fertilized ovum of an animal using the carrier 审中-公开用于共培养动物的受精卵的载体和使用载体培养动物的受精卵的方法公开(公告)号：US20050164159A1
公开(公告)日：2005-07-28
申请号：US11038253
申请日：2005-01-21
CPC分类号： C12N5/0068 , C12N5/0604 , C12N2533/54
摘要： There is provided a carrier for co-culturing a fertilized ovum of an animal by which behavior of the fertilized ovum of an animal can be easily observed in a culture system and by which adhesion and three-dimensional growth of the fertilized ovum become possible at first, as well as there is provided a method of culturing the fertilized ovum of an animal using the co-culturing carrier. There is provided a carrier for co-culturing a fertilized ovum of an animal composed of a cell incorporated type three-dimensionally reconstructed tissue for co-culturing the fertilized ovum of an animal to induce adhesion and three-dimensional growth, of the fertilized ovum as well as there is provided a method of culturing the fertilized ovum of an animal characterized in that the co-culturing carrier is introduced into a culture vessel to culture the fertilized ovum of an animal.
摘要翻译： 提供了用于共培养动物受精卵的载体，通过该载体可以容易地在培养系统中观察到动物的受精卵的行为，并且首先使受精卵的粘附和三维生长成为可能 ，并且提供了使用共培养载体培养动物的受精卵的方法。 提供了一种用于共培养动物受精卵的载体，该动物由细胞结合型三维重建组织组成，用于共培养动物的受精卵以诱导受精卵的粘附和三维生长作为 因为提供了培养动物的受精卵的方法，其特征在于将共培养载体引入培养容器中以培养动物的受精卵。
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公开(公告)号：US07195912B2
公开(公告)日：2007-03-27
申请号：US11035954
申请日：2005-01-18
CPC分类号： A61L31/145 , A61L31/005 , A61L31/044 , C12N5/0068 , C12N2533/54
摘要： The thin film of the invention comprises a hydrate of a vitrified gel containing one or more extracellular matrix components, which can be integrated with a retainer as required. A hydrogel thin film containing one or more extracellular matrix components such as thin-film collagen hydrogel thin film, which is useful for a cell culture substratum and for preventing organ adhesion, can be easily prepared, and is excellent in expediency.
摘要翻译： 本发明的薄膜包含含有一种或多种细胞外基质组分的玻璃化凝胶的水合物，其可以根据需要与保持剂一体化。 可以容易地制备含有一种或多种细胞外基质成分的水凝胶薄膜，例如可用于细胞培养基底和用于防止器官粘连的薄膜胶原蛋白水凝胶薄膜，并且便利性优异。
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公开(公告)号：US20050129720A1
公开(公告)日：2005-06-16
申请号：US11035954
申请日：2005-01-18
CPC分类号： A61L31/145 , A61L31/005 , A61L31/044 , C12N5/0068 , C12N2533/54
摘要： The thin film of the invention comprises a hydrate of a vitrified gel containing one or more extracellular matrix components, which can be integrated with a retainer as required. A hydrogel thin film containing one or more extracellular matrix components such as thin-film collagen hydrogel thin film, which is useful for a cell culture substratum and for preventing organ adhesion, can be easily prepared, and is excellent in expediency.
摘要翻译： 本发明的薄膜包含含有一种或多种细胞外基质组分的玻璃化凝胶的水合物，其可以根据需要与保持剂一体化。 可以容易地制备含有一种或多种细胞外基质成分的水凝胶薄膜，例如可用于细胞培养基底和用于防止器官粘连的薄膜胶原蛋白水凝胶薄膜，并且便利性优异。
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6.发明授权Medium-penetrating cell culture carrier, a culturing method and a device using this carrier 失效中等渗透性细胞培养载体，培养方法和使用该载体的装置
公开(公告)号：US5736399A
公开(公告)日：1998-04-07
申请号：US401255
申请日：1995-03-09
CPC分类号： C12M25/14 , C12N5/0068 , C12N2533/54
摘要： The present invention provides a medium-penetrating culture carrier comprising a plurality of natural or synthetic threads or the woven body thereof, a method for adhering cells onto this carrier to allow them to be proliferated, and a device which is physically connected to the carrier for feeding a medium using this medium-penetrating culture carrier, which are able to culture three-dimensionally animal cells in order that they can effect self-assembly as they do in the living tissue or organ from which they are derived.
摘要翻译： 本发明提供一种中等穿透性培养载体，其包含多个天然或合成丝线或其编织体，一种将细胞粘附到载体上以使其增殖的方法，以及物理连接到载体的装置 使用这种中等穿透性培养载体喂养培养基，其能够三维培养动物细胞，以便它们可以像在活体组织或其衍生物的器官中一样实现自组装。
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7.发明申请CELL CULTURE CHAMBER, METHOD FOR PRODUCING SAME, TISSUE MODEL USING CELL CULTURE CHAMBER, AND METHOD FOR PRODUCING SAME 审中-公开细胞培养室，其生产方法，使用细胞培养室的组织模型及其生产方法公开(公告)号：US20130280807A1
公开(公告)日：2013-10-24
申请号：US13884651
申请日：2011-11-10
IPC分类号： G01N33/50
CPC分类号： G01N33/5008 , C12M21/08 , C12M23/10 , C12M25/04 , C12M25/14
摘要： A single cell-culture chamber is provided that includes a dried vitrigel membrane covering and secured to one open end surface of a tubular frame. Also provided is a double cell-culture chamber that includes two tubular frames of substantially the same planar cross-sectional shape adhesively secured to each other with a dried vitrigel membrane interposed between the opposing open end surfaces of the tubular frames so as to form a first chamber and a second chamber via the dried vitrigel membrane.
摘要翻译： 提供单个细胞培养室，其包括覆盖并固定到管状框架的一个开放端表面的干燥的玻璃质凝胶膜。 还提供了一种双细胞培养室，其包括两个基本上相同的平面截面形状的管状框架，其通过插入在管状框架的相对开口端表面之间的干燥玻璃凝胶膜彼此粘合地固定，以形成第一 室和通过干燥的玻璃体膜的第二室。
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8.发明申请DRIED HYDROGEL, DRIED VITRIGEL MEMBRANE, AND METHODS FOR PRODUCING THE SAME 审中-公开干燥水凝胶，干燥维生素膜及其制造方法公开(公告)号：US20130217126A1
公开(公告)日：2013-08-22
申请号：US13818759
申请日：2011-08-25
CPC分类号： B29D7/01 , B29C39/02 , B29C41/02 , B29K2089/00 , C12M25/14 , C12N5/0062
摘要： A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.
摘要翻译： 干燥玻璃凝胶膜是通过以下步骤制造的：（1）将水凝胶保持在壁表面模具内部的形状与设置在基板上的所需形状相同的形状的步骤， 水凝胶内的自由水从基材和壁表面模具之间的间隙中释放出来; （2）从基板的顶部除去壁面模具的工序; （3）干燥水凝胶以去除残留游离水的步骤，由此制造玻璃化干燥水凝胶; （4）将干燥的水凝胶再水化以制造玻璃el膜的步骤; 和（5）重新搅拌玻璃浆膜以除去游离水的步骤，从而制造玻璃化的干燥玻璃凝胶膜。
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公开(公告)号：US5985539A
公开(公告)日：1999-11-16
申请号：US112211
申请日：1998-07-09
申请人： Toshiaki Takezawa , Nobuo Hanai
发明人： Toshiaki Takezawa , Nobuo Hanai
CPC分类号： A01N1/0226 , A01N1/02 , C12N5/0062 , C12N5/0671 , A61K35/12
摘要： The present invention relates to a method for reconstruction of animal organ which comprises perfusing a cell-dispersible solution to animal organ from vascular system and then perfusing a cell culture medium whereby the organ is reconstructed.According to the present invention, the organ can be reconstructed and cultured for long term without separating most of cells which constitute the animal organ.
摘要翻译： 本发明涉及一种动物器官重建方法，其特征在于，将来自血管系统的细胞分散溶液灌注到动物器官中，然后灌注细胞培养基，由此重建器官。 根据本发明，器官可以长期重建和培养，而不分离构成动物器官的大部分细胞。
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