Novel superoxide dismutase and process for preparation thereof
    6.
    发明授权
    Novel superoxide dismutase and process for preparation thereof 失效
    新型超氧化物歧化酶及其制备方法

    公开(公告)号:US4774185A

    公开(公告)日:1988-09-27

    申请号:US915026

    申请日:1986-10-03

    IPC分类号: C12N9/02 C12R1/01

    CPC分类号: C12N9/0089 Y10S435/822

    摘要: A novel superoxide dismutase prepared from a superoxide dismutase-producing strain of the genus Nocardiopsis. The enzyme is a tetramer with a molecular weight of about 8.8.times.10.sup.4, composed of sub-units of about 2.2.times.10.sup.4. The superoxide dismutase contains 1.56 g-atoms of iron per mole but no copper or manganese. Potassium cyanide at a concentration of 1 mM does not inhibit activity, however exponential inactivation is caused by 5 mM hydrogen peroxide.

    摘要翻译: 一种由Nocardiopsis属超氧化物歧化酶生产菌株制备的新型超氧化物歧化酶。 该酶是分子量约为8.8×10 4的四聚物,由约2.2×10 4的亚单位组成。 超氧化物歧化酶每摩尔含有1.56克原子铁,但不含铜或锰。 浓度为1mM的氰化钾不抑制活性,但是由5mM过氧化氢引起指数失活。

    Process for preparing lipase
    8.
    发明授权
    Process for preparing lipase 失效
    脂肪酶的制备方法

    公开(公告)号:US4791059A

    公开(公告)日:1988-12-13

    申请号:US844389

    申请日:1986-03-17

    IPC分类号: C12N9/20 C12P7/64 C12R1/44

    摘要: This invention relates to a process for preparation of a novel lipase. The process is characterized by culturing a microorganism belonging to Staphylococcus capitis and capable of producing lipase in a culture medium to accumulate lipase in the cultured broth and recovering the lipase from the broth. Since the pH of this lipase is in a slightly alkali region and the lipase substantially completely converts glyceride to fatty acids and glycerin, it is useful for the economic preparation of fatty acids by the hydrolysis of fats or oils.

    摘要翻译: PCT No.PCT / JP85 / 00409 Sec。 371日期:1986年3月17日 102(e)1986年3月17日PCT申请日1985年7月19日PCT公布。 第WO86 / 00925号公报 日本1986年2月13日。本发明涉及新型脂肪酶的制备方法。 该方法的特征在于培养属于葡萄球菌属的炎症微生物,并且能够在培养基中产生脂肪酶以在培养的肉汤中积聚脂肪酶并从肉汤中回收脂肪酶。 由于该脂肪酶的pH处于微碱性区域,脂肪酶基本上完全将甘油酯转化为脂肪酸和甘油,因此通过脂肪或油的水解来经济地制备脂肪酸是有用的。