公开(公告)号：US07169592B2

公开(公告)日：2007-01-30

申请号：US10112706

申请日：2002-04-02

申请人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Keiji Matsumoto ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

发明人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Keiji Matsumoto ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

IPC分类号： C12N9/10 ,  C12N1/21 ,  C12N15/54 ,  C12N15/63 ,  C12N15/82 ,  C12N15/85 ,  C07H21/04

CPC分类号： C12P13/001 ,  C12N9/1096

摘要： To provide a method for preparing optically active compounds, which mainly contain (R)-amino compounds, by microbial enzymes efficiently and inexpensively; a polypeptide having stereoselective transaminase activity which can be suitably used for the above preparation method; and a DNA encoding the polypeptide. A method for preparing an optically active amino compound, characterized in stereoselectively transaminating by acting a transaminase on an amino group acceptor, a ketone compound in the presence of an amino group donor, a primary amine; a DNA comprising a nucleotide sequence encoding a polypeptide having stereoselective transaminase activity; and a polypeptide having stereoselective transaminase activity obtainable from a culture of a microorganism belonging to the genus Arthrobacter.

摘要翻译： 提供通过微生物酶有效且廉价地制备主要含有（R） - 氨基化合物的光学活性化合物的方法; 具有立体选择性转氨酶活性的多肽，可适用于上述制备方法; 和编码该多肽的DNA。 一种制备光学活性氨基化合物的方法，其特征在于通过在氨基受体上起转氨酶的作用，在氨基供体存在下的酮化合物，伯胺，立体选择性转氨酶; 包含编码具有立体选择性转氨酶活性的多肽的核苷酸序列的DNA; 和具有立体选择性转氨酶活性的多肽，其可从属于节杆菌属的微生物的培养物获得。

公开(公告)号：US06221638B1

公开(公告)日：2001-04-24

申请号：US09064750

申请日：1998-04-23

申请人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

发明人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

IPC分类号： C12P1300

CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12P13/005 ,  C12P13/008 ,  C12P13/04 ,  C12P41/006 ,  Y10S435/83

摘要： The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.

摘要翻译： 制备光学活性（R） - 氨基化合物的方法，其特征在于在酮化合物（氨基受体）的共存下通过（R） - 形式 - 特异性转氨酶的作用立体选择性地进行氨基转移的方法， 和外消旋形式的氨基化合物（氨基供体）或（R） - 形式，得到光学活性（R） - 氨基化合物。 根据本发明，可以容易地容易地制备其1-位上具有芳基等的光学活性（R） - 氨基化合物等，其通常难以制备。

公开(公告)号：US06344351B2

公开(公告)日：2002-02-05

申请号：US09794359

申请日：2001-02-28

申请人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

发明人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

IPC分类号： C12N910

CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12P13/005 ,  C12P13/008 ,  C12P13/04 ,  C12P41/006 ,  Y10S435/83

摘要： The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.

摘要翻译： 制备光学活性（R） - 氨基化合物的方法，其特征在于在酮化合物（氨基受体）的共存下通过（R） - 形式 - 特异性转氨酶的作用立体选择性地进行氨基转移的方法， 和外消旋形式的氨基化合物（氨基供体）或（R） - 形式，得到光学活性（R） - 氨基化合物。 根据本发明，可以容易地容易地制备其1-位上具有芳基等的光学活性（R） - 氨基化合物等，其通常难以制备。

公开(公告)号：US06346402B1

公开(公告)日：2002-02-12

申请号：US09582764

申请日：2000-06-30

申请人： Akira Iwasaki ,  Yukio Yamada ,  Yasuhiro Ikenaka ,  Yoshihiko Yasohara ,  Junzo Hasegawa

发明人： Akira Iwasaki ,  Yukio Yamada ,  Yasuhiro Ikenaka ,  Yoshihiko Yasohara ,  Junzo Hasegawa

IPC分类号： C12P1304

CPC分类号： C12N9/1096 ,  C12P41/006

摘要： Relating to an enzyme capable of efficiently converting a ketone compound to an optically active amino compound by transamination, and a process for preparing an optically active amino compound using the enzyme. An (S)-&agr;-phenethylamine:pyruvate transaminase, which acts on (S)-&agr;-phenethylamine and a ketone compound, thereby catalyzing transamination for forming acetophenone and an amino compound corresponding to the ketone compound; a process for preparing an optically active amino compound using the transaminase; and a method for culturing a microorganism for producing the above transaminase, comprising adding to a medium one or more compounds selected from the group consisting of propylamine, 1-butylamine, 2-butylamine, 2-pentylamine, isopropylamine and isobutylamine as an inducer for the enzyme when a microorganism for producing (S)-&agr;-phenethylamine:pyruvate transaminase is cultured.

摘要翻译： 关于能够通过转氨酶有效地将酮化合物转化为光学活性氨基化合物的酶，以及使用该酶制备光学活性氨基化合物的方法。 （S）-α-苯乙胺：丙酮酸转氨酶，其作用于（S）-α-苯乙胺和酮化合物，从而催化转氨酶形成苯乙酮和对应于酮化合物的氨基化合物; 使用转氨酶制备光学活性氨基化合物的方法; 以及培养用于制备上述转氨酶的微生物的方法，包括向培养基中加入一种或多种选自丙胺，1-丁胺，2-丁胺，2-戊胺，异丙胺和异丁胺的化合物作为诱导剂 用于生产（S）-α-苯乙胺：丙酮酸转氨酶的微生物培养时的酶。

公开(公告)号：US5223647A

公开(公告)日：1993-06-29

申请号：US902734

申请日：1992-06-23

申请人： Shunich Maemoto ,  Akira Iwasaki ,  Junzo Hasegawa

发明人： Shunich Maemoto ,  Akira Iwasaki ,  Junzo Hasegawa

IPC分类号： C07C67/56 ,  C07B57/00 ,  C07C45/78 ,  C07C45/85 ,  C07C49/497 ,  C07C49/517 ,  C07C67/58 ,  C07C69/76

CPC分类号： C07C45/78 ,  C07C45/85

摘要： The present invention discloses a separating and purifying (4R, 6R)-4-hydroxy-2,2,6-trimethyl cyclohexanone comprising the steps of:reacting a diastereomer mixture of (4R, 6R)-4-hydroxy-2,2,6-trimethyl cyclohexanone represented by the formula (I) ##STR1## and (4S, 6R)-4-hydroxy-2,2,6-trimethyl cyclohexanone represented by the formula (II) ##STR2## with a reagent which reacts with the hydroxyl groups at least equimolar to the compound represented by the formula (II) to thereby convert the compound represented by the formula (II) into (4S, 6R)-4-hydroxy-2,2,6-trimethyl cyclohexanone derivative represented by the formula (III) ##STR3## (where R represents a group modifying the hydroxyl group) by modification with priority of the hydroxyl group in the formula (II), andseparating the compound of the formula (I) from the compound of the formula (III) through the difference in solubility to water or an organic solvent. According to the invention, (4R, 6R)-4-hydroxy-2,2,6-trimethyl cyclohexanone is easily separated with a high yield.

公开(公告)号：US20130030164A1

公开(公告)日：2013-01-31

申请号：US13574458

申请日：2011-01-19

申请人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

发明人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

IPC分类号： C12N9/02 ,  C12N15/63 ,  C07H21/02 ,  C12N1/19 ,  C12N5/10 ,  C12P19/36 ,  C12N15/53 ,  C12N1/21

CPC分类号： C12N9/0036 ,  C12N9/0065 ,  C12P7/02 ,  C12P7/18 ,  C12P7/42 ,  C12P13/04 ,  C12P19/36 ,  C12Y106/03 ,  C12Y106/03001 ,  C12Y111/01001

摘要： Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.

摘要翻译： 来自变形链球菌的成水NADH氧化酶在工业生产实际使用的稳定性方面应进一步提高。 本发明的目的是提供通过蛋白质工程技术，通过改性水合NADH氧化酶获得的酶，其可用作由氧化还原酶催化的用于立体选择性氧化的NAD +再生系统，使得酶能够承受 长期使用而不显示其NAD +再生活性的活性降低，即稳定性提高的酶，以及提供有效生产光学活性醇或氨基酸等有用物质的方法。 本发明涉及通过适当地引入突变来提高来自变形链球菌的水形成NADH氧化酶的稳定性的酶修饰方法。

公开(公告)号：US06513424B1

公开(公告)日：2003-02-04

申请号：US09647897

申请日：2000-10-06

申请人： Akira Iwasaki ,  Toshinari Sasaki ,  Shigetoshi Kashiwagi ,  Shigemi Isobe

发明人： Akira Iwasaki ,  Toshinari Sasaki ,  Shigetoshi Kashiwagi ,  Shigemi Isobe

IPC分类号： B30B1116

CPC分类号： B30B11/16 ,  B30B11/006 ,  B30B11/18 ,  B30B15/0017 ,  B30B15/0082 ,  B30B15/308

摘要： A pair of compression rollers 38a and 38b parallel with each other are provided and powder grains are supplied to a powder grain introduction/compression part 50 formed between the rollers 38a and 38b and, thereby, compression moldings of the powder grains are formed. A powder grain press/feed means 20 is provided in a front stage of the rollers 38a and 38b. The press/feed means 20 has a deaerating barrel 24 and previously presses the powder grains supplied between the rollers 38a and 38b. In side surfaces of the rollers 38a and 38b, side seals 37 are arranged with clearances 72 maintained from the rollers 38a and 38b. During pressing the powder moldings, the powder grains enter into the clearances so that closer layers are formed between the side surfaces of the compression rollers 38a and 38b and the side seals 37, thereby sealing the powder grain introduction/compression part 50. In a rear stage of the rollers 38a and 38b, a shearing device 75 is provided and shears the compression moldings formed by the rollers 38a and 38b. Torque of the shearing device 75 is detected by a torque sensor, and the compression rollers 38a and 38b and the powder grain transport means 17 are controlled in accordance with the detected torque.

摘要翻译： 设置一对彼此平行的压缩辊38a和38b，并且将粉末颗粒供给到形成在辊38a和38b之间的粉末颗粒引入/压缩部分50，从而形成粉末颗粒的压缩模制品。 在辊38a和38b的前段设置有粉粒挤压/进给装置20。 压榨/进料装置20具有脱气筒24并预先挤压供应在辊38a和38b之间的粉末颗粒。 在辊38a和38b的侧表面中，侧密封件37布置有从辊38a和38b保持的间隙72。 在挤压粉末成型体期间，粉粒进入间隙，使得在压缩辊38a和38b的侧表面与侧密封件37之间形成更近的层，从而密封粉粒引入/压缩部50.在后部 辊38a和38b的阶段，设置有剪切装置75，并且剪切由辊38a和38b形成的压缩模制品。 通过转矩传感器检测剪切装置75的扭矩，根据检测的转矩来控制压缩辊38a，38b和粉粒传送装置17。

公开(公告)号：US06291557B1

公开(公告)日：2001-09-18

申请号：US09695975

申请日：2000-10-26

申请人： Kyoichi Yamamoto ,  Kojun Utaka ,  Hisashi Takada ,  Akira Iwasaki

发明人： Kyoichi Yamamoto ,  Kojun Utaka ,  Hisashi Takada ,  Akira Iwasaki

IPC分类号： C08K310

CPC分类号： C09J163/00 ,  C08G59/4292

摘要： The invention provides an alkali-soluble adhesive agent characterized by comprising an alkali-soluble resin that is a reaction product (C) obtained by allowing a polycarboxylic acid and/or a polycarboxylic anhydride having two or more carboxyl groups to react with a reaction product of a compound (A) having at least one epoxy group with a monocarboxylic acid compound (B). Preferably, the alkali-soluble resin is used in combination with a ketone organic solvent, optionally with at least one member selected from the group consisting of a melt viscosity reducing agent, a surface active agent and a plasticizer. The alkali-soluble adhesive agent can be removed by an alkali aqueous solution without recourse to any organic solvent, and is of an environmental protection-conscious type with improvements in adhesion, adhesion strength, coatability, surface smoothness, softening point controllability, melt viscosity controllability and spread controllability.

摘要翻译： 本发明提供一种碱溶性粘合剂，其特征在于包含碱溶性树脂，其为通过使多羧酸和/或具有两个或更多个羧基的多元羧酸酐与下述反应产物反应获得的反应产物（C） 具有至少一个环氧基的化合物（A）与单羧酸化合物（B）。 优选地，碱溶性树脂与酮有机溶剂组合使用，任选地与选自熔体粘度降低剂，表面活性剂和增塑剂中的至少一种组合使用。 碱溶性粘合剂可以通过碱水溶液除去而不用任何有机溶剂，并且具有环保意识型，其粘合性，粘合强度，可涂布性，表面平滑性，软化点可控性，熔体粘度可控性 并传播可控性。

公开(公告)号：US5906704A

公开(公告)日：1999-05-25

申请号：US937463

申请日：1997-09-25

申请人： Kazushige Matsuura ,  Akira Iwasaki ,  Hideki Ohmori

发明人： Kazushige Matsuura ,  Akira Iwasaki ,  Hideki Ohmori

IPC分类号： A41D27/06 ,  B29C41/18 ,  C08G18/10 ,  C08G18/80 ,  C08K5/16 ,  C08K5/21 ,  C08L75/04 ,  C09J175/04 ,  C09K3/00 ,  C08G18/40 ,  C08G18/66 ,  C08K5/205

CPC分类号： C08G18/10 ,  C09J175/04 ,  C08G2170/20

摘要： The present invention provides a hot-melt resin composition which comprises 1 to 30% by weight of a compound represented by the general formula (1)Q-(X-A)n (1)(wherein X is a urethane or urea group, Q is a group with a valence of n, A is a univalent group, one of Q and A being an isocyanate residue and the other being an amine or alcohol residue, and n is an integer of 1 to 3) and 70 to 99% by weight of a thermoplastic polyurethane resin (B); a method of producing a powder form of said hot-melt resin composition; and a hot-melt resin powder composition which comprises said powder-form hot-melt resin composition.

摘要翻译： 本发明提供一种热熔树脂组合物，其包含1至30重量％的由通式（1）表示的化合物Q-（XA）n（1）（其中X为氨基甲酸酯或脲基，Q为 化合价为n的基团，A为一价基团，Q和A之一为异氰酸酯残基，另一个为胺或醇残基，n为1〜3的整数），70〜99重量％ 的热塑性聚氨酯树脂（B）; 一种生产所述热熔树脂组合物的粉末形式的方法; 和包含所述粉末状热熔树脂组合物的热熔树脂粉末组合物。

公开(公告)号：US4706977A

公开(公告)日：1987-11-17

申请号：US922040

申请日：1986-10-21

申请人： Norio Ukai ,  Akira Iwasaki

发明人： Norio Ukai ,  Akira Iwasaki

IPC分类号： B62D7/15 ,  B62D7/00

CPC分类号： B62D7/1527

摘要： A steering device for steering front and rear wheels of a motor vehicle has a steering gear box for the front wheels and a steering gear box for the rear wheels. The front wheel steering gear box includes a first rack shaft operatively coupled to the front wheels, a first input shaft and an output shaft operatively coupled to the first rack shaft. The rear wheel steering gear box includes a second rack shaft operatively coupled to the rear wheels and a second input shaft operatively coupled to the second rack shaft. The output shaft and the second input shaft are interconnected by a linkage shaft. The second input shaft has an end inserted into the rear wheel steering gear box and inclined downwardly. The rear wheel steering gear box is supported on the vehicle frame for tilting movement around the second rack shaft.

摘要翻译： 用于转向机动车辆的前轮和后轮的转向装置具有用于前轮的转向齿轮箱和用于后轮的转向齿轮箱。 前轮舵机箱包括可操作地联接到前轮的第一齿条轴，第一输入轴和可操作地联接到第一齿条轴的输出轴。 后轮舵机包括可操作地联接到后轮的第二齿条轴和可操作地联接到第二齿条轴的第二输入轴。 输出轴和第二输入轴通过联动轴相互连接。 第二输入轴具有插入后轮舵机箱中并向下倾斜的一端。 后轮舵机箱被支撑在车架上用于围绕第二齿条轴倾斜运动。