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    Novel FRT recombination sites and methods of use
    7.
    发明申请
    Novel FRT recombination sites and methods of use 有权
    新型FRT重组位点和使用方法

    公开(公告)号：US20080047031A1

    公开(公告)日：2008-02-21

    申请号：US11487300

    申请日：2006-07-14

    申请人： Yumin Tao ,  Dennis Bidney ,  William Gordon-Kamm ,  Leszek Lyznik

    发明人： Yumin Tao ,  Dennis Bidney ,  William Gordon-Kamm ,  Leszek Lyznik

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C12N5/04 ,  C40B40/02 ,  C12N5/10 ,  C12N1/00

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

    Modified FRT recombination site libraries and methods of use
    8.
    发明申请
    Modified FRT recombination site libraries and methods of use 审中-公开
    修改的FRT重组位点文库和使用方法

    公开(公告)号：US20070015195A1

    公开(公告)日：2007-01-18

    申请号：US11487273

    申请日：2006-07-14

    申请人： Yumin Tao ,  Dennis Bidney ,  William Gordon-Kamm ,  Leszek Lyznik

    发明人： Yumin Tao ,  Dennis Bidney ,  William Gordon-Kamm ,  Leszek Lyznik

    IPC分类号： C40B30/06 ,  C40B50/06

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 还提供了包含FRT位点的文库群的试剂盒，以及制备修饰的FRT重组位点的文库的方法。 重组生成修饰的FRT重组位点可用于多种目标多重核苷酸重组方法。

    Compostions and Methods for Identifying Transformed Cells
    9.
    发明申请
    Compostions and Methods for Identifying Transformed Cells 审中-公开
    识别转化细胞的组合和方法

    公开(公告)号：US20080032309A1

    公开(公告)日：2008-02-07

    申请号：US11869771

    申请日：2007-10-10

    申请人： Keith Lowe ,  William Gordon-Kamm ,  George Hoerster

    发明人： Keith Lowe ,  William Gordon-Kamm ,  George Hoerster

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6895 ,  C12Q2600/13

    摘要： The present invention relates to compositions and methods for identifying transformed cells. The method comprises introducing a visual marker polynucleotide into a plant cell and providing a growth stimulation protein. The compositions comprise a visual marker polynucleotide and a growth stimulation polynucleotide. Also provided are expression cassettes, plant cells, plant parts, and plants comprising same.

    摘要翻译： 本发明涉及鉴定转化细胞的组合物和方法。 该方法包括将视觉标记多核苷酸引入植物细胞并提供生长刺激蛋白。 组合物包含视觉标记多核苷酸和生长刺激多核苷酸。 还提供了表达盒，植物细胞，植物部分和包含它们的植物。

    Seed-preferred regulatory elements and uses thereof
    10.
    发明申请
    Seed-preferred regulatory elements and uses thereof 失效
    种子优选的调节元件及其用途

    公开(公告)号：US20070220626A1

    公开(公告)日：2007-09-20

    申请号：US11519184

    申请日：2006-09-11

    申请人： Shane Abbitt ,  William Gordon-Kamm ,  Keith Lowe

    发明人： Shane Abbitt ,  William Gordon-Kamm ,  Keith Lowe

    IPC分类号： C07H21/04 ,  A01H1/00 ,  C12N15/63 ,  A01H5/00

    CPC分类号： C12Y505/01004 ,  C07K14/415 ,  C12N9/90 ,  C12N15/8234

    摘要： The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for callus-tissue and seed embryo-preferred regulatory sequences. Methods for expressing an isolated nucleotide sequence in a plant or callus tissue using the regulatory sequences are also provided. The methods comprise transforming a plant cell to contain an isolated nucleotide sequence operably linked to the regulatory sequences of the present invention and regenerating a stably transformed plant or callus tissue from the transformed plant cell.

    摘要翻译： 本发明提供用于调节植物中分离的核苷酸序列表达的组合物和方法。 组合物是用于愈伤组织和种子胚优选调节序列的新型核酸序列。 还提供了使用调节序列在植物或愈伤组织中表达分离的核苷酸序列的方法。 所述方法包括将植物细胞转化成含有与本发明的调控序列可操作地连接的分离的核苷酸序列，并从转化的植物细胞再生稳定转化的植物或愈伤组织。