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公开(公告)号：US20210041428A1
公开(公告)日：2021-02-11
申请号：US17069591
申请日：2020-10-13
发明人： Robert F. Zuk , Hong Tan , Qing Xia , Pu Li , Haode Chen , Heng Wu
IPC分类号： G01N33/543 , G01N33/533 , G01N33/53 , G01N33/58
摘要： The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.
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公开(公告)号：US10451616B2
公开(公告)日：2019-10-22
申请号：US15429362
申请日：2017-02-10
发明人： Robert F. Zuk
IPC分类号： G01N33/543 , G01N21/76 , G01N21/66 , G01N33/58 , G01N33/53
摘要： The present invention relates to a method for quantitating an analyte having a wide range concentration in a single assay without having to dilute the sample and repeating the assay. The key feature of the invention is having two cycles of events including sample binding to probe, binding reactions, and detection. After the first cycle of binding and detecting, the probe is dipped into the same sample vessel to bind additional analyte in the sample vessel at a condition that is more favorable to binding than the condition in the first cycle.
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公开(公告)号：US09804179B2
公开(公告)日：2017-10-31
申请号：US15293173
申请日：2016-10-13
发明人： Hong Tan , Ming Xia , Yushan Tan , Jun Chen , Erhua Cao , Genqian Li , Robert F. Zuk
CPC分类号： G01N35/00 , B01L3/502 , B01L3/527 , B01L2200/16 , B01L2300/042 , B01L2300/045 , G01N33/54353 , G01N33/54366 , G01N35/026 , G01N2035/0436
摘要： This invention relates to an apparatus for conducting immunoassay test. The apparatus includes a groove unit having a groove along a vertical direction configured to hold a rod-shaped portion of a probe along the vertical direction, and a push pin configured to move along a horizontal direction, the push pin being capable of residing at a first position and a second position. A tip of the push pin is capable of pressing the rod-shaped portion of the probe against the groove when the push pin resides at the first position. The distance between the tip of the push pin and the groove is larger than a diameter of the rod-shaped portion of the probe when the push pin resides at the second position.
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4.发明授权Luminescent immunoassays for quantitating analytes having a wide concentration range 有权用于定量浓度范围宽的分析物的发光免疫测定公开(公告)号：US09568431B2
公开(公告)日：2017-02-14
申请号：US13794080
申请日：2013-03-11
发明人： Robert F. Zuk
IPC分类号： G01N33/543 , G01N21/76 , G01N21/66 , G01N33/58
CPC分类号： G01N33/54306 , G01N21/66 , G01N21/76 , G01N33/5308 , G01N33/582 , G01N2458/30 , G01N2458/40
摘要： The present invention relates to a method for quantitating an analyte having a wide range concentration in a single assay without having to dilute the sample and repeating the assay. The key feature of the invention is having two cycles of events including sample binding to probe, binding reactions, and detection. After the first cycle of binding and detecting, the probe is dipped into the same sample vessel to bind additional analyte in the sample vessel at a condition that is more favorable to binding than the condition in the first cycle.
摘要翻译： 本发明涉及用于在单次测定中定量具有宽范围浓度的分析物的方法，而不必稀释样品并重复测定。 本发明的关键特征是具有两个循环的事件，包括样品结合到探针，结合反应和检测。 在结合和检测的第一个循环之后，将探针浸入相同的样品容器中，以比第一次循环中的条件更有利于结合的条件将样品容器中的另外的分析物结合。
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公开(公告)号：USD759268S1
公开(公告)日：2016-06-14
申请号：US29537860
申请日：2015-08-28
设计人： Hong Tan , Genqian Li , Ming Xia , Yongli Ren
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公开(公告)号：US20140128596A1
公开(公告)日：2014-05-08
申请号：US13846517
申请日：2013-03-18
发明人： Robert F. ZUK
IPC分类号： C08B37/00
CPC分类号： G01N33/54366 , C08B37/006 , G01N21/6428 , G01N21/645 , G01N33/54373 , G01N2021/6439 , G01N2201/06113
摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.
摘要翻译： 本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。 该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。 本发明还涉及一种使用具有小表面积（＆NlE; 5mm）的探针尖端和具有多个结合分子和多个荧光标记的高分子量聚合物（≥1MD）来检测液体样品中的分析物的方法。 通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。 本发明进一步涉及包含具有多个结合分子和多个荧光标记的交联FICOLL®分子的荧光标记组合物。
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公开(公告)号：US08309369B2
公开(公告)日：2012-11-13
申请号：US13224174
申请日：2011-09-01
申请人： Hong Tan , Robert F. Zuk , Yushan Tan , Erhua Cao , Min Xia , Jun Chen
发明人： Hong Tan , Robert F. Zuk , Yushan Tan , Erhua Cao , Min Xia , Jun Chen
IPC分类号： G01N33/543
CPC分类号： G01N33/54366 , C08B37/006 , G01N21/6428 , G01N21/645 , G01N33/54373 , G01N2021/6439 , G01N2201/06113
摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.
摘要翻译： 本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。 该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。 本发明还涉及一种使用具有小表面积（＆NlE; 5mm）的探针尖端和具有多个结合分子和多个荧光标记的高分子量聚合物（≥1MD）来检测液体样品中的分析物的方法。 通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。 本发明还涉及包含具有多个结合分子和多个荧光标记的交联Ficoll分子的荧光标记组合物。
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公开(公告)号：US20240272153A1
公开(公告)日：2024-08-15
申请号：US18628497
申请日：2024-04-05
发明人： Haode Chen , Robert F. Zuk
IPC分类号： G01N33/543 , G01N21/64 , G01N33/533
CPC分类号： G01N33/54393 , G01N21/6428 , G01N33/533
摘要： The present invention is directed biochemical assay methods with regenerated substrate surface for immunoassay reactions. The methods clean a ceramic or glass substrate surface with atmospheric gas plasma and then coat the cleaned surface directly (without further modification of the cleaned surface) with a hapten-polymer conjugate, a protein, or an anti-non-human IgG antibody as a first layer on the substrate surface for subsequence assay reactions. After the completion of each cycle of reactions, the substrate is cleaned by the same plasma cleaning procedure, to elute the organic molecules formed on the substrate surface and to regenerate the substrate for another cycle of immunoassay reactions. The regeneration protocols can be repeated 1-10 times and maintains acceptable assay performance.
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公开(公告)号：US20240082836A1
公开(公告)日：2024-03-14
申请号：US18517999
申请日：2023-11-22
发明人： Hong Tan , Haode Chen , Genqian Li
IPC分类号： B01L3/00
CPC分类号： B01L3/502715 , B01L2200/04 , B01L2300/0663 , B01L2300/0861 , B01L2400/043
摘要： Introduced here are rotors that can be placed inside of microplate wells that include liquid samples. Each rotor can be comprised of a ferromagnetic material. Accordingly, when a rotor is subjected to an external rotational magnetic field, the rotor spins and agitates the liquid sample inside the corresponding well. The spin speed may be adjusted by changing the rotation speed, direction, and/or orientation of the external rotational magnetic field. The rotor typically includes a central cavity within which a probe can be suspended during the biochemical test.
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10.发明公开公开(公告)号：US20240044886A1
公开(公告)日：2024-02-08
申请号：US17754473
申请日：2020-10-07
IPC分类号： G01N33/543 , G01N33/557
CPC分类号： G01N33/54373 , G01N33/557
摘要： The present invention is directed to biochemical assay methods, which re-use a hapten-immobilized test probe and reagents for quantitating an analyte or measuring kinetic binding in different samples, anywhere from about 3 to 20 times, while maintaining acceptable assay performance. The methods use a conjugate solution either comprising (i) an anti-hapten antibody and a capture antibody against an analyte, or (ii) an anti-hapten antibody and streptavidin, in each cycle and regenerate the hapten-coated test probe by dipping the test probe in an acidic solution having pH about 1-4, optionally further dipping in a DMSO solution, after the completion of each cycle of reaction. The robustness of the hapten-immobilized solid phase allows utilization of denaturation reagents for efficient elution of the immune complexes after each cycle without compromising the binding activity of the hapten on the solid phase.
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