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    Novel protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof
    1.
    发明申请
    Novel protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof 有权
    新型蛋白质脱酰胺酶，编码基因的基因及其制备方法及其用途

    公开(公告)号：US20040166558A1

    公开(公告)日：2004-08-26

    申请号：US10815751

    申请日：2004-04-02

    申请人： AMANO PHARMACEUTICAL CO., LTD.

    发明人： Shotaro Yamaguchi ,  Akira Matsuura

    IPC分类号： C12P021/06 ,  C07H021/04 ,  C12N009/52 ,  C12N001/20

    CPC分类号： A23J3/34 ,  C12N9/80

    摘要： A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.

    摘要翻译： 一种具有通过作用于蛋白质中侧链酰胺基的蛋白质释放侧链羧基和氨的活性的新型酶。 本发明涉及一种酶的制备方法，其包括在培养基中培养属于细胞毒细菌或放线菌属的细菌的菌株，并且具有产生具有使蛋白质中酰氨基化的性质的酶的能力，由此 影响所述酶的产生，并随后从培养混合物中收集所述酶。 本发明还涉及使用直接作用于蛋白质中的酰胺基的新型酶的蛋白质的制造方法，以及具有使蛋白质中的酰氨基脱氨基化的酶和编码所述酶的基因的酶。

    Bread quality-improving composition and bread producing process using the same
    2.
    发明授权
    Bread quality-improving composition and bread producing process using the same 失效
    面包质量改善成分和面包生产过程使用相同

    公开(公告)号：US5698245A

    公开(公告)日：1997-12-16

    申请号：US454565

    申请日：1995-05-30

    申请人： Noriaki Tanaka ,  Kuniharu Nakai ,  Kenichi Takami ,  Yoshiyuki Takasaki

    发明人： Noriaki Tanaka ,  Kuniharu Nakai ,  Kenichi Takami ,  Yoshiyuki Takasaki

    IPC分类号： A21D6/00 ,  A21D8/04 ,  A21D2/00

    CPC分类号： A21D8/042

    摘要： A bread quality-improving composition and a process for the production of bread using the composition. More particularly, a bread quality-improving composition which contains a maltotriose-forming enzyme or a maltotriose-forming enzyme, glucose oxidase and/or hemicellulase and a process for the production of dough and bread using the composition. The bread quality-improving composition renders possible the easy handling of dough without covering it with wheat flour and the like, the exclusion of additives for use in the control of water content, an improvement in elasticity and ductility of bread when baked, the prevention of bread solidification and other problems such as a reduction in bread volume when frozen dough is used and the elimination or reduction of emulsifying agents.

    摘要翻译： 面包质量改善组合物和使用该组合物生产面包的方法。 更具体地，包含形成麦芽三糖的酶或麦芽三糖形成酶，葡萄糖氧化酶和/或半纤维素酶的面包质量改进组合物和使用该组合物生产面团和面包的方法。 面包质量改进的组合物使面团的容易处理成为可能，而不用小面粉等覆盖面包，排除用于控制含水量的添加剂，烘烤时面包的弹性和延展性的改善，防止 面包凝固和其他问题，例如当使用冷冻面团时减少面包体积并消除或减少乳化剂。

    Triterpenyl esters of organic acids and hypolipidemic agents composed of them
    4.
    发明授权
    Triterpenyl esters of organic acids and hypolipidemic agents composed of them 失效
    有机酸的三萜烯酯和由它们组成的降血脂剂

    公开(公告)号：US4748161A

    公开(公告)日：1988-05-31

    申请号：US739183

    申请日：1985-05-30

    申请人： Goro Kimura ,  Yoshihiko Hirose ,  Kumi Yoshida ,  Fumio Kuzuya ,  Katsunari Fujita

    发明人： Goro Kimura ,  Yoshihiko Hirose ,  Kumi Yoshida ,  Fumio Kuzuya ,  Katsunari Fujita

    IPC分类号： C07J53/00 ,  C07J9/00

    CPC分类号： C07J53/004

    摘要： A triterpenyl ester of organic acid other than triterpenyl esters of ferulic acid and of monobasic and dibasic saturated fatty acids. A process for producing a triterpenyl ester of organic acid other than esters of ferulic acid and of monobasic and dibasic saturated fatty acids, which comprises the reaction of a triterpenyl alcohol with an acid halide of the corresponding organic acid. A pharmaceutical composition for treatment of hyperlipidemia comprising a pharmaceutical carrier and an effective amount of a triterpenyl ester of organic acid other than triterpenyl esters of dibasic saturated fatty acids. A pharmaceutical composition for treating hyperlipidemia which comprises a pharmaceutical carrier and an effective amount of cyclobranol as an active ingredient.

    摘要翻译： 除了阿魏酸和一元和二元饱和脂肪酸的三萜烯酯以外的有机酸的三萜烯酯。 一种制备除阿魏酸和一元和二元饱和脂肪酸以外的有机酸三萜烯酯的方法，其包括三萜烯醇与相应有机酸的酰卤反应。 一种用于治疗高脂血症的药物组合物，其包含药物载体和有效量的除二元饱和脂肪酸的三萜烯酯之外的有机酸的三萜烯基酯。 一种用于治疗高脂血症的药物组合物，其包含药物载体和有效量的环唑啉作为活性成分。

    Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof
    6.
    发明授权
    Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof 有权
    蛋白质脱酰胺酶，编码基因的基因，其制备方法及其用途

    公开(公告)号：US07279298B2

    公开(公告)日：2007-10-09

    申请号：US10815751

    申请日：2004-04-02

    申请人： Shotaro Yamaguchi ,  Akira Matsuura

    发明人： Shotaro Yamaguchi ,  Akira Matsuura

    IPC分类号： C12Q1/00 ,  C12Q1/48

    CPC分类号： A23J3/34 ,  C12N9/80

    摘要： A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.

    摘要翻译： 一种具有通过作用于蛋白质中侧链酰胺基的蛋白质释放侧链羧基和氨的活性的新型酶。 本发明涉及一种酶的制备方法，其包括在培养基中培养属于细胞毒细菌或放线菌属的细菌的菌株，并且具有产生具有使蛋白质中酰氨基化的性质的酶的能力，由此 影响所述酶的产生，并随后从培养混合物中收集所述酶。 本发明还涉及使用直接作用于蛋白质中的酰胺基的新型酶的蛋白质的制造方法，以及具有使蛋白质中的酰氨基脱氨基化的酶和编码所述酶的基因的酶。

    Process of making cyclodextrin glucanotransferase with brevibacterium
    8.
    发明授权
    Process of making cyclodextrin glucanotransferase with brevibacterium 失效
    用短杆菌制备环糊精葡聚糖转移酶的方法

    公开(公告)号：US5707833A

    公开(公告)日：1998-01-13

    申请号：US471235

    申请日：1995-06-06

    申请人： Shigeharu Mori ,  Tamio Mase ,  Takaichi Ohya

    发明人： Shigeharu Mori ,  Tamio Mase ,  Takaichi Ohya

    IPC分类号： C12N9/10 ,  C12P19/18 ,  C12P21/04

    CPC分类号： C12N9/1074 ,  C12P19/18 ,  Y10S435/84

    摘要： This invention relates to a .gamma.-cyclodextrin glucanotransferase having novel properties, to a process for the production of the .gamma.-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the .gamma.-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of .gamma.-cyclodextrin as a main product, and to a method for increasing .gamma.-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which .gamma.-cyclodextrin and .beta.-cyclodextrin are formed from starch by the action of .gamma.-cyclodextrin glucanotransferase, thereby effecting repressed formation of .beta.-cyclodextrin and enhanced formation of .gamma.-cyclodextrin. Development of the process of this invention has rendered possible low cost production of .gamma.-cyclodextrin and therefore expands practical use of .gamma.-cyclodextrin to the field of food and the like.

    摘要翻译： 本发明涉及一种具有新特性的γ-环糊精葡聚糖转移酶，涉及γ-环糊精葡聚糖转移酶的制备方法，该方法包括培养能产生环糊精葡聚糖转移酶的短杆菌属属的菌株，从而使菌株产生γ- 环糊精葡聚糖转移酶，然后将酶收集到生产环糊精的方法中，该方法包括使环糊精葡聚糖转移酶与溶解在溶液中的底物反应，从而形成γ-环糊精作为主要产物，并且 一种增加γ-环糊精产率而不伴随产生总环糊精的增加的方法，该方法包括向γ-淀粉酶通过γ-环糊精葡聚糖转移酶的作用从淀粉形成γ-环糊精和β-环糊精的反应溶液中加入乙醇， 形成β-环糊精并增强γ-环糊精的形成。 本发明方法的开发使得γ-环糊精的低成本生产成为可能，从而将γ-环糊精的实际应用扩展到食品领域等。

    Process for mannose and mannose isomerase production using mannose isomerase-producing pseudomonas
    9.
    发明授权
    Process for mannose and mannose isomerase production using mannose isomerase-producing pseudomonas 失效
    使用产甘露糖异构酶的假单胞菌生产甘露糖和甘露糖异构酶的方法

    公开(公告)号：US5240717A

    公开(公告)日：1993-08-31

    申请号：US851665

    申请日：1992-03-16

    申请人： Yoshiyuki Takasaki ,  Takaichi Ohya

    发明人： Yoshiyuki Takasaki ,  Takaichi Ohya

    IPC分类号： C12N9/90

    CPC分类号： C12R1/38 ,  C12N9/90 ,  Y10S435/874

    摘要： A mannose isomerase having excellent properties for industrial use, such as high thermal stability and resistance to high substrate concentrations, can be produced by culturing a strain of Pseudomonas (sp. AM-9582), and extracting it from the cells of AM-9582. Mannose can be effectively produced from fructose of high concentrations using the enzyme.

    摘要翻译： 可以通过培养假单胞菌（SP.AM-9582）菌株并从AM-9582的细胞中提取具有优异工业性质的甘露糖异构酶，例如高热稳定性和高底物浓度。 使用酶可以从高浓度的果糖有效地制备甘露糖。

    Process for modifying fats and oils
    10.
    发明授权
    Process for modifying fats and oils 失效
    改性油脂的方法

    公开(公告)号：US5219744A

    公开(公告)日：1993-06-15

    申请号：US722388

    申请日：1991-06-26

    申请人： Jun Kurashige ,  Narihide Matsuzaki ,  Tamio Mase ,  Shotaro Yamaguchi

    发明人： Jun Kurashige ,  Narihide Matsuzaki ,  Tamio Mase ,  Shotaro Yamaguchi

    IPC分类号： C12N9/20 ,  C12P7/64

    CPC分类号： C12P7/6454 ,  C12N9/20 ,  Y10S435/933

    摘要： A process for modifying fats and oils which comprises applying partial glyceride lipase to fats and oils containing partial glycerides and fatty acids to synthesize triglycerides from the partial glycerides by esterification.The lipase is preferably an immobilized lipase and this process is preferably carried out under a water concentration of 1500 ppm or less.By using partial glyceride lipase according to the present invention, there can be obtained modified fats and oils having a low content of diglycerides without changing triglyceride composition.

    摘要翻译： 一种改性脂肪和油的方法，其包括将偏甘油酯脂肪酶应用于含有偏甘油酯和脂肪酸的脂肪和油，以通过酯化从所述部分甘油酯合成甘油三酯。 脂肪酶优选为固定化脂肪酶，该方法优选在1500ppm以下的水分浓度下进行。 通过使用根据本发明的偏甘油酯脂肪酶，可以获得具有低甘油二酯含量的改性脂肪和油，而不改变甘油三酯组成。