公开(公告)号：US20100264030A1

公开(公告)日：2010-10-21

申请号：US12705200

申请日：2010-02-12

申请人： German Arciniegas ,  Eric Wallace ,  Daniel Y. Chu ,  Jason McKee

发明人： German Arciniegas ,  Eric Wallace ,  Daniel Y. Chu ,  Jason McKee

IPC分类号： C25B9/02 ,  C25B7/00

CPC分类号： G01N27/44704

摘要： Horizontal electrophoresis is performed in a cell that includes a tank and a pair of electrodes, each mounted on an electrode support that is easily inserted into and removed from the tank, and once inserted, is secured in position by one or more spring-loaded, manual-release locking features.

摘要翻译： 在包括容器和一对电极的电池中进行水平电泳，每个电极安装在容易插入和移出罐的电极支撑件上，并且一旦插入，就通过一个或多个弹簧加载的固定在适当位置， 手动释放锁定功能。

公开(公告)号：US20100041122A1

公开(公告)日：2010-02-18

申请号：US12477701

申请日：2009-06-03

申请人： Charles W. Ragsdale

发明人： Charles W. Ragsdale

IPC分类号： C12N13/00 ,  C12M1/00

CPC分类号： C12M33/00 ,  C12M35/02

摘要： Membrane-encased structures such as biological cells, liposomes, and vesicles, are conveyed through one or more channels in a rotating disk for individual exposure to optical elements or to electrodes, for purposes of transfection or flow cytometry. The rotation of the disk serves either to provide centrifugal force to urge the cells against one wall of the channel and in certain embodiments to move the cells through the channels, or to draw cells at preselected times or intervals into the exposure zone, or all three.

摘要翻译： 为了转染或流式细胞术的目的，诸如生物细胞，脂质体和囊泡的膜包裹的结构通过旋转盘中的一个或多个通道被输送以单独暴露于光学元件或电极。 盘的旋转用于提供离心力以将细胞推动到通道的一个壁上，并且在某些实施例中以使细胞移动通过通道，或者以预选的时间或间隔将细胞拉入曝光区域，或者全部三个 。

公开(公告)号：US20100028988A1

公开(公告)日：2010-02-04

申请号：US12511839

申请日：2009-07-29

申请人： Daniel Y. Chu ,  Hui Du

发明人： Daniel Y. Chu ,  Hui Du

IPC分类号： C12M1/34 ,  B29C55/00

CPC分类号： B29C51/04 ,  B01L3/50851 ,  B01L2200/12 ,  B01L2300/0829 ,  B29C51/02 ,  B29C2791/006 ,  B29K2023/06 ,  B29K2023/12

摘要： A multi-well plate with wells having ultra-thin walls and yet with sufficient structural rigidity to allow reliable use of the plate in automated instrumentation is formed by first forming a plate blank by injection molding, the blank being of a thickness that provides the desired rigidity, and then subjecting the blank to vacuum forming to stretch designated areas in the blank to form wells or to extend wells already formed, the stretching resulting in a reduction in thickness of the molded resin at the walls of the wells only.

摘要翻译： 具有超薄壁并且具有足够的结构刚性以允许在自动化仪器中可靠地使用板的多孔板通过首先通过注射成型形成板坯而形成，坯料具有提供期望的厚度 刚性，然后对坯料进行真空成形以拉伸坯料中的指定区域以形成孔或延伸已经形成的孔，拉伸仅导致在孔壁处的模制树脂的厚度减小。

公开(公告)号：US20070202018A1

公开(公告)日：2007-08-30

申请号：US11361411

申请日：2006-02-24

申请人： Daniel Chu ,  Paul Patt ,  Jeffry Ceremony

发明人： Daniel Chu ,  Paul Patt ,  Jeffry Ceremony

IPC分类号： B01L9/00

CPC分类号： G01N35/028 ,  B01L9/523 ,  B01L2200/025 ,  B01L2300/0829

摘要： A platform that supports a sample plate such as a microtiter plate, a multi-well plate of any size, or a glass slide with sample spots distributed over its surface, and presents the plate for assay detection by a movable scanning head that has a field depth on the millimeter scale is leveled or otherwise adjusted in a planar orientation by an apparatus that includes a rocker plate, position sensors, and motorized risers arranged on the apparatus to provide the rocker plate with tilting capability along either or both or two orthogonal axes.

摘要翻译： 支撑样品板的平台，例如微量滴定板，任何尺寸的多孔板或具有分布在其表面上的样品斑点的玻片，并且通过具有场的可移动扫描头呈现用于测定检测的板 通过包括设置在设备上的摇臂板，位置传感器和电动立管的装置，平面定位或以其它方式调整深度，以使摇杆具有沿着一个或两个或两个正交轴线的倾斜能力。

公开(公告)号：US20060223187A1

公开(公告)日：2006-10-05

申请号：US11099361

申请日：2005-04-04

申请人： Franklin Carver ,  James Lapicola

发明人： Franklin Carver ,  James Lapicola

IPC分类号： G01N33/48

CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25

摘要： Erythrocytes from a vertebrate animal other than a human that have been size reduced and treated with a fixing agent by conventional methods to resemble human platelets are further treated to achieve a size distribution that is detected in a consistent manner by different methodologies of blood cell counting. The further treatment includes heating to a denaturing temperature followed by a second treatment with a fixing agent. The resulting cells are useful as controls for calibrating and checking the accuracy of automated blood cell counting equipment.

公开(公告)号：US20050059761A1

公开(公告)日：2005-03-17

申请号：US10973541

申请日：2004-10-25

申请人： Cory Panattoni

发明人： Cory Panattoni

IPC分类号： C08F2/38 ,  C08F2/10 ,  C08F4/00 ,  C08F4/40 ,  C08F20/56 ,  C08F220/56 ,  C08K5/17 ,  G01N27/447 ,  C08K3/30 ,  C08K3/00

CPC分类号： G01N27/44747 ,  C08F220/56 ,  C08K5/17 ,  G01N27/44704 ,  Y10S524/916 ,  Y10S526/934 ,  C08L33/26

摘要： Irregularities in the pore structures of polyacrylamide gels that are formed in cassettes against plastic walls are reduced or eliminated by the inclusion of an oxygen scavenger in the gel-forming solution. Avoidance of the irregularities results in electropherograms with fewer distortions in the solute bands.

摘要翻译： 通过在凝胶形成溶液中包含氧清除剂，减少或消除了在塑料壁上形成的聚丙烯酰胺凝胶的孔结构中的不规则性。 避免不规则性导致电泳图在溶质带中具有较少的失真。

公开(公告)号：US20090209017A1

公开(公告)日：2009-08-20

申请号：US12366440

申请日：2009-02-05

申请人： Charles W. Ragsdale

发明人： Charles W. Ragsdale

IPC分类号： C12N13/00 ,  C12M1/42

CPC分类号： C12M25/02 ,  C12M35/02 ,  C12N13/00

摘要： An electroporation cuvette is constructed with electroporation electrodes arranged in non-parallel relation to form a gap whose width varies with the location within the cuvette, plus a pair of positioning electrodes that are arranged to cause electrophoretic migration of biological cells within the cuvette according to cell size. Once the cells, suspended in a solution of the impregnant, are distributed in the cuvette by the positioning electrodes, electric field pulses are generated by the non-parallel electroporation electrodes. Because of their distribution in the cuvette, the various cells will experience voltage differentials across their widths that approach uniformity regardless of cell diameter, since the larger cells will be positioned at locations where the gap between the electrodes is greater and the smaller cells at locations where the gap is relatively small while the voltage drop across the entire gap is uniform along the length of the cell. The voltage differential across the width of the cell is thus roughly paired with the cell diameter, and this reduces the disparity in voltage differential that cells of different sizes would otherwise experience with parallel electrodes.

摘要翻译： 电穿孔试管是以不平行关系排列的电穿孔电极构成的，以形成宽度随试剂杯内部位置而变化的间隙，另外还有一对定位电极，它们被设置成根据电池使生物细胞在比色杯内电泳迁移 尺寸。 一旦悬浮在浸渍剂溶液中的细胞通过定位电极分布在反应杯中，则电场脉冲由非平行电穿孔电极产生。 由于它们在比色皿中的分布，各种单元将经历其宽度上的电压差，其接近均匀度而不考虑单元直径，因为较大的单元将位于电极之间的间隙较大的位置处，而较小的单元位于 间隙相对较小，而跨整个间隙的电压降沿着电池的长度是均匀的。 因此，电池宽度上的电压差与电池直径大致配对，这样可以减小电压差异，不同尺寸的电池将以平行电极的方式经历。

公开(公告)号：US20070096568A1

公开(公告)日：2007-05-03

申请号：US11265000

申请日：2005-11-01

申请人： Paul Patt ,  Daniel Chu ,  Jeffry Ceremony

发明人： Paul Patt ,  Daniel Chu ,  Jeffry Ceremony

IPC分类号： H02K35/00 ,  H02K33/00

CPC分类号： H02K33/18

摘要： A moving coil actuator that moves in a rapid back-and-forth motion is constructed with a magnet assembly that concentrates the magnetic flux in a region of limited length and with a coiled electric conductor that includes two separated regions of densely wound coil, the assembly and conductor arranged such that only one of the coil regions resides in a concentrated flux region at either end of the actuator stroke. This is achieved with either a single region of concentrated flux or two spatially separated regions of concentrated flux. In either case, the force constant, i.e., the motor force generated in each coil per unit of current through the coil, is non-linear, with a maximal force at each end of the stroke and a minimal force at the stroke mid-point. The result is an efficient use of the electric current and relatively small amounts of materials of construction, and accordingly less weight for the actuator to carry during its travel and its changes of direction.

公开(公告)号：US20070065074A1

公开(公告)日：2007-03-22

申请号：US11521677

申请日：2006-09-14

申请人： James Hillendahl ,  David Waldbeser

发明人： James Hillendahl ,  David Waldbeser

IPC分类号： G02B6/32

CPC分类号： G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

摘要： Biochemical assays of samples in receptacles, in gels, in blots, in arrays and the like, and that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the sample. Emission light produced by the excitation is then collected by a lens and converted to a signal that is compiled by conventional software for analysis. The optical fiber transfixes (passes through) the lens via a slot or other opening and is preferably offset from the center of the lens. The optical fiber and collecting lens can either be on the same side of the sample or on opposite sides, i.e., one above and the other below. The optical fiber and the light collecting lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle. Illumination systems are also disclosed in which a ultraviolet, visible, or near-infrared light source is optically coupled to an optical fiber.

摘要翻译： 通过照射和检测系统增强了在容器，凝胶，印迹，阵列等中的样品和利用激发和发光作为检测标签的样品的生物化学分析，其通过透射激发光的光纤提供激发光 从激发光源到样品。 然后由透镜产生的发射光被透镜收集并转换成由常规软件编译的信号用于分析。 光纤经由狭槽或其他开口将透镜经过（透过）透镜，并且优选地偏离透镜的中心。 光纤和收集透镜可以在样品的相同侧或者在相对的两侧，即一个在另一个之上。 光纤和聚光透镜被布置成使得激发光的行进方向和发射光被收集的方向不同轴，并且优选地两者都与垂直于嘴的轴成锐角 容器 还公开了照明系统，其中紫外线，可见光或近红外光源光学耦合到光纤。

公开(公告)号：US20050155862A1

公开(公告)日：2005-07-21

申请号：US10763405

申请日：2004-01-21

申请人： Lee Olech

发明人： Lee Olech

IPC分类号： C07C279/12 ,  G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44795 ,  C07C279/12 ,  G01N27/44747

摘要： Carboxylic acid-substituted polyalkylene polyamines in which amine nitrogen atoms on the polyamine backbone structure are replaced by guanidine groups provide a pH range extending into high pH values. These substances are useful as carrier ampholytes in isoelectric focusing.

摘要翻译： 羧胺取代的聚亚烷基多胺，其中多胺骨架结构上的胺氮原子被胍基团取代，提供了延伸到高pH值的pH范围。 这些物质可用作等电聚焦中的载体两性电解质。