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    Artificial binding proteins based on a modified alpha helical region of ubiquitin
    1.
    发明授权
    Artificial binding proteins based on a modified alpha helical region of ubiquitin 有权
    基于泛素修饰的α螺旋区的人工结合蛋白

    公开(公告)号：US08592179B2

    公开(公告)日：2013-11-26

    申请号：US12514550

    申请日：2007-11-15

    申请人： Michael Schraeml ,  Erik Fiedler

    发明人： Michael Schraeml ,  Erik Fiedler

    IPC分类号： C07K14/435

    CPC分类号： C07K14/00 ,  G01N33/6803

    摘要： The present invention is directed to a method for the generation of binding proteins derived from the protein super-family of ubiquitin like proteins with modifications in their alpha helical region as well as to a protein obtainable by said method. Furthermore, the invention provides the use of a protein for the specific recognition, binding and neutralization of a predescribed target molecule, for the detection, quantitative determination, separation and/or for the isolation of a corresponding binding partner and the use of a protein of the invention, for diagnosis, prophylaxis and treatment of diseases in which the corresponding binding partner is directly or indirectly involved.

    摘要翻译： 本发明涉及一种用于产生衍生自泛蛋白样蛋白质的蛋白质超家族的结合蛋白质的方法，其修饰在其α螺旋区域以及可通过所述方法获得的蛋白质。 此外，本发明提供蛋白质用于预测的靶分子的特异性识别，结合和中和用于检测，定量测定，分离和/或分离相应结合配偶体的用途以及使用蛋白质 本发明用于诊断，预防和治疗其中相应的结合伴侣直接或间接参与的疾病。

    METHOD FOR PRODUCING RECOMBINANT THROMBIN
    2.
    发明申请
    METHOD FOR PRODUCING RECOMBINANT THROMBIN 审中-公开
    生产重组血红蛋白的方法

    公开(公告)号：US20130052715A1

    公开(公告)日：2013-02-28

    申请号：US13641844

    申请日：2011-04-20

    申请人： Andreas Anton ,  Arndt Dietrich ,  Jochen Koetter ,  Joerg Schaeffner

    发明人： Andreas Anton ,  Arndt Dietrich ,  Jochen Koetter ,  Joerg Schaeffner

    IPC分类号： C12N9/74

    CPC分类号： C12N9/6429 ,  C12Y304/21005

    摘要： The invention relates to a method for producing folded prethrombin, wherein inclusion bodies, which contain unfolded prethrombin or a derivative thereof, are solubilized in a solubilization buffer containing at least one chaotropic compound and at least one organic disulfide compound. The invention further relates to methods for producing thrombin and a-thrombin and derivatives thereof. The invention also relates to solutions that contain folded proteins, which can be produced by the methods according to the invention.

    摘要翻译： 本发明涉及一种生产折叠凝血酶原的方法，其中包含未折叠凝血酶原或其衍生物的包涵体溶解于含有至少一种离液序列化合物和至少一种有机二硫化物的增溶缓冲液中。 本发明还涉及产生凝血酶和α-凝血酶及其衍生物的方法。 本发明还涉及可以通过根据本发明的方法生产的折叠蛋白质的溶液。

    MIXTURE OF AT LEAST TWO FUSION PROTEINS, THE PRODUCTION THEREOF AND THE USE OF THE SAME
    5.
    发明申请
    MIXTURE OF AT LEAST TWO FUSION PROTEINS, THE PRODUCTION THEREOF AND THE USE OF THE SAME 有权
    至少两种融合蛋白的混合物，其生产及其使用

    公开(公告)号：US20120015819A1

    公开(公告)日：2012-01-19

    申请号：US13222272

    申请日：2011-08-31

    申请人： Matthias PASCHKE

    发明人： Matthias PASCHKE

    IPC分类号： C40B10/00 ,  C40B30/04

    CPC分类号： C40B30/04 ,  C07K2319/10 ,  C07K2319/60 ,  C07K2319/73 ,  C07K2319/81 ,  C12N15/1037 ,  C12N15/62 ,  C40B40/02 ,  G01N2500/04 ,  G01N2500/10

    摘要： The present invention concerns a protein mixture comprising at least a first fusion protein comprising a protein or protein fragment, and an interaction domain and a protein translocation sequence, which effects that the fusion protein upon expression in a bacterium is translocated through the cytoplasmic membrane in an essentially unfolded state and at least a second fusion protein comprising a protein or protein fragment, and an interaction domain and a protein translocation sequence which effects that the fusion protein is translocated through the cytoplasmic membrane upon expression in a bacterium in an essentially folded state, wherein the interaction domain of the first protein can bind to those of the second protein.

    摘要翻译： 本发明涉及包含至少包含蛋白质或蛋白质片段的第一融合蛋白以及相互作用结构域和蛋白质易位序列的蛋白质混合物，所述互作用结构域和蛋白质易位序列影响在细菌中表达时的融合蛋白通过细胞质膜被转位 基本上解折叠状态，以及至少第二融合蛋白，其包含蛋白质或蛋白质片段，以及相互作用结构域和蛋白质易位序列，其在基本折叠状态下在细菌中表达时影响融合蛋白被转位穿过细胞质膜，其中 第一蛋白质的相互作用结构域可以结合第二蛋白质的相互作用结构域。

    Method for obtaining active pro-NGF and beta-NGF
    8.
    发明授权
    Method for obtaining active pro-NGF and beta-NGF 有权
    获得活性前NGF和β-NGF的方法

    公开(公告)号：US08501439B2

    公开(公告)日：2013-08-06

    申请号：US12765229

    申请日：2010-04-22

    申请人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    发明人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    IPC分类号： C12N15/18

    CPC分类号： C07K14/48

    摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.

    摘要翻译： 本发明涉及从前体proNGF生产生物活性β-NGF的方法。 在原核宿主细胞中表达β-NGF的形式后，以不溶性无活性聚集体（包涵体）的形式分离重组蛋白。 在将其溶解在强变性剂中并随后转化成通过存在于天然β-NGF中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性β-NGF。

    Pharmaceutical preparations comprising human proNGF
    10.
    发明授权
    Pharmaceutical preparations comprising human proNGF 有权
    包含人类proNGF的药物制剂

    公开(公告)号：US08318671B1

    公开(公告)日：2012-11-27

    申请号：US09807096

    申请日：1999-10-11

    申请人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    发明人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    IPC分类号： C07K14/48 ,  A61K38/18

    CPC分类号： C07K14/48

    摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.

    摘要翻译： 本发明涉及从前体proNGF生产生物活性的NGF的方法。 在原核宿主细胞中表达生物素-NGF的形式后，重组蛋白以不溶性非活性聚集体（包涵体）的形式分离。 在将其溶解在强变性剂中并随后转化成通过存在于天然和/或生长因子中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性 。