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113 条记录 (耗时 0.048 秒)

    Side-wall heater for thermocycler device
    4.
    发明授权
    Side-wall heater for thermocycler device 有权
    用于热循环装置的侧壁加热器

    公开(公告)号：US07459302B2

    公开(公告)日：2008-12-02

    申请号：US10261751

    申请日：2002-10-01

    申请人： Taylor Reid ,  Roger Taylor ,  Larry Brown

    发明人： Taylor Reid ,  Roger Taylor ,  Larry Brown

    IPC分类号： C12M1/38

    CPC分类号： B01L7/52 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/1805 ,  Y10S435/809

    摘要： The invention relates to an apparatus and methods for preventing condensation on the interior surfaces of sample tubes which are being exposed to temperature cycles, such as during a PCR amplification reaction. In particular, the invention relates to apparatus comprising a sample block comprising a plurality of sample wells for receiving sample tubes and heating elements disposed in the sample wells for heating at least a portion of the sides of sample tubes (e.g., at least the portion which forms the head space after a tube is filled with a PCR reaction mixture). In a preferred aspect, the sample block is part of a thermocycling device for performing PCR and the side-wall heater is used to enhance uniformity and speed of amplification reactions. For example, by decreasing or eliminating condensation, signal strength jumps in a real-time PCR assay can be minimized as can reaction non-homogeneity.

    摘要翻译： 本发明涉及一种用于防止在诸如PCR扩增反应期间暴露于温度循环的样品管的内表面上的冷凝的装置和方法。 特别地，本发明涉及包括样品块的装置，其包括多个用于接收样品管的样品孔和设置在样品阱中的加热元件，用于加热样品管的至少一部分侧面（例如，至少部分 在管填充PCR反应混合物后形成顶部空间）。 在优选的方面，样品块是用于进行PCR的热循环装置的一部分，并且侧壁加热器用于增强扩增反应的均匀性和速度。 例如，通过减少或消除冷凝，实时PCR测定中的信号强度跳跃可以如反应非均匀性那样最小化。

    Compositions and methods for the detection of a nucleic acid using a cleavage reaction
    5.
    发明申请
    Compositions and methods for the detection of a nucleic acid using a cleavage reaction 审中-公开

    公开(公告)号：US20080008995A1

    公开(公告)日：2008-01-10

    申请号：US11296614

    申请日：2005-12-07

    申请人： Joseph Sorge

    发明人： Joseph Sorge

    IPC分类号： C12Q1/68 ,  C12P21/00

    CPC分类号： C12Q1/6823 ,  C12Q1/6813 ,  C12Q2561/109 ,  C12Q2537/163 ,  C12Q2525/161 ,  C12Q2521/301

    摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid sequence with a nucleic acid polymerase and cleaving the cleavage structure with a nuclease to generate a cleaved nucleic acid fragment. The invention also relates to methods of detecting or measuring a target nucleic acid sequence, where the method includes forming a cleavage structure by incubating a target nucleic acid sequence with a nucleic acid polymerase, cleaving the cleavage structure with a nuclease and detecting or measuring the release of a fragment.

    Polymerase compositions and uses thereof
    6.
    发明授权
    Polymerase compositions and uses thereof 失效
    聚合酶组合物及其用途

    公开(公告)号：US07312052B1

    公开(公告)日：2007-12-25

    申请号：US08529767

    申请日：1995-09-18

    申请人： Joseph A. Sorge ,  Rebecca Lynn Mullinax

    发明人： Joseph A. Sorge ,  Rebecca Lynn Mullinax

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02

    CPC分类号： C12Q1/686 ,  C12Q2521/319 ,  C12Q2521/101

    摘要： The subject invention provides novel compositions containing a mixture of (a) an enzyme that possesses substantial 3′–5′ exonuclease activity (b) a DNA polymerase with less 3′–5′ exonuclease activity than the enzyme with substantial 3′–5′ exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially lack 3′–5′ exonuclease activity. A preferred embodiment of the invention is a composition comprising the Taq DNA polymerase (isolated from Thermus aquaticus) and the Pfu DNA polymerase (isolated from Pyrococcus furiosus). Another aspect of the invention is to provide methods for synthesizing polynucleotides, typically DNA, using compositions comprising an enzyme that possesses substantial 3′–5′ exonuclease activity and a DNA polymerase with less 3′–5′ exonuclease activity than the enzymes possessing substantial 3′–5′ exonuclease activity, preferably a DNA polymerase that substantially lacks 3′–5′ exonuclease activity. Another aspect of the invention involves the use the subject method of polynucleotide synthesis to carry out the synthesis step in a polymerase chain reaction experiment. Yet another aspect of the invention is to provide kits for the synthesis of polynucleotides, wherein the kits comprise an enzyme that possesses substantial 3′–5′ exonuclease activity and a DNA polymerase with less 3′–5′ exonuclease activity than the enzyme possessing substantial 3′–5′ exonuclease activity.

    摘要翻译： 本发明提供了包含（a）具有大量3'-5'核酸外切酶活性的酶的混合物的新型组合物（b）具有比实际3'-5'核酸外切酶活性大的3'-5'核酸外切酶活性的3' 核酸外切酶活性。 优选地，包含在组合物中的DNA聚合酶是基本上缺乏3'-5'核酸外切酶活性的DNA聚合酶。 本发明的优选实施方案是包含Taq DNA聚合酶（从Thermus aquaticus分离）和Pfu DNA聚合酶（从激烈热球菌分离的）的组合物。 本发明的另一方面是提供使用包含具有大量3'-5'核酸外切酶活性的酶的组合物和具有比具有实质3的酶的3'-5'外切核酸酶活性少的DNA聚合酶合成多核苷酸（通常为DNA）的方法 '-5'核酸外切酶活性，优选基本上缺乏3'-5'核酸外切酶活性的DNA聚合酶。 本发明的另一方面涉及使用聚合酶链反应实验中的多核苷酸合成的主题方法进行合成步骤。 本发明的另一方面是提供用于合成多核苷酸的试剂盒，其中所述试剂盒包含具有显着3'-5'核酸外切酶活性的酶和具有比具有实质性的酶的酶更少3'-5'外切核酸酶活性的DNA聚合酶 3'-5'核酸外切酶活性。

    Methods of enriching for and identifying polymorphisms
    8.
    发明申请
    Methods of enriching for and identifying polymorphisms 审中-公开

    公开(公告)号：US20070259371A1

    公开(公告)日：2007-11-08

    申请号：US11786361

    申请日：2007-04-11

    申请人： Joseph Sorge ,  Ronald Davis

    发明人： Joseph Sorge ,  Ronald Davis

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2565/519 ,  C12Q2565/531 ,  C12Q2533/101 ,  C12Q2522/101

    摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.

    Methods of enriching for and identifying polymorphisms
    9.
    发明申请
    Methods of enriching for and identifying polymorphisms 有权

    公开(公告)号：US20070248979A1

    公开(公告)日：2007-10-25

    申请号：US11786204

    申请日：2007-04-11

    申请人： Joseph Sorge ,  Ronald Davis

    发明人： Joseph Sorge ,  Ronald Davis

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2565/519 ,  C12Q2565/531 ,  C12Q2533/101 ,  C12Q2522/101

    摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.