公开(公告)号：US20100272709A1

公开(公告)日：2010-10-28

申请号：US12693523

申请日：2010-01-26

申请人： Jochen Reiser ,  Sanja Sever

发明人： Jochen Reiser ,  Sanja Sever

IPC分类号： A61K39/395 ,  A61K31/7088 ,  A61K38/02 ,  A61K38/46 ,  A61K38/16 ,  A61P3/10 ,  A61P9/12 ,  A61P9/00 ,  C12N5/00 ,  C12Q1/34 ,  C12Q1/68

CPC分类号： A61K38/07 ,  A61K38/02 ,  A61K38/05 ,  A61K38/46 ,  A61K38/55 ,  C12Q1/37 ,  G01N2333/96466 ,  G01N2500/02

摘要： This invention relates generally to the treatment of cathepsin or dynamin mediated diseases, such as proteinuria, cancer, and cognitive disease and related products. Diagnostic and other assays are also provided, as well as methods for podocyte cell gene transfer.

摘要翻译： 本发明一般涉及组织蛋白酶或dynamin介导的疾病如蛋白尿，癌症和认知疾病及相关产品的治疗。 还提供了诊断和其他测定，以及足细胞细胞基因转移的方法。

公开(公告)号：US07547538B2

公开(公告)日：2009-06-16

申请号：US10400060

申请日：2003-03-27

申请人： Azorides Morales ,  Harold Essenfeld ,  Ervin Essenfeld

发明人： Azorides Morales ,  Harold Essenfeld ,  Ervin Essenfeld

IPC分类号： A01N1/00

CPC分类号： B01L9/50 ,  G01N1/06 ,  G01N1/30 ,  G01N1/312 ,  G01N1/36 ,  G01N1/44 ,  G01N2001/305 ,  G01N2001/315

摘要： A process and apparatus for rapid, continuous flow histological processing of tissues is disclosed. The steps of fixation, dehydration, clearing and impregnation are performed in less than one hour; this allows a pathologist to evaluate samples shortly after receipt, perhaps while the patient is still in the operating room. Rapid and continuous processing is accomplished by decreasing the thickness of tissue sections, use of nonaqueous solutions composed of admixtures of solutions, solution exchange at elevated temperature and with agitation, and impregnation under vacuum pressure. The patient in surgery is thus provided with point-of-care surgical pathology.

摘要翻译： 公开了用于组织快速，连续流组织学处理的方法和装置。 固定，脱水，清洗和浸渍的步骤在不到一小时的时间内完成; 这允许病理学家在收到后不久评估样本，也许在患者仍在手术室时。 通过减少组织切片的厚度，使用由溶液的混合物组成的非水溶液，在升高的温度下进行溶液交换和搅拌，以及在真空压力下浸渍来实现快速和连续的加工。 因此，手术中的患者提供了点护理手术病理学。

公开(公告)号：US07219884B2

公开(公告)日：2007-05-22

申请号：US10260443

申请日：2002-10-01

申请人： Azorides R. Morales

发明人： Azorides R. Morales

IPC分类号： B23Q1/00

CPC分类号： G01N1/286 ,  G01N2001/061 ,  Y10T83/04 ,  Y10T83/0448 ,  Y10T83/6945

摘要： A tool for holding tissue to be sampled in place to allow tissue samples of a prescribed thickness to be prepared from the held tissue. The tool includes at least one tine array for engaging and holding the tissue. First and second tine arrays may advantageously be provided to define a slice thickness therebetween. In addition, or in the alternative, the tines of the tine array are mutually spaced apart by different distances to effectively hold the tissue and to define prescribed tissue sample lengths.

摘要翻译： 用于将要采样的组织保持在适当位置以允许从所保持的组织制备规定厚度的组织样本的工具。 该工具包括用于接合和保持组织的至少一个齿阵列。 可有利地提供第一和第二齿阵列以在其间限定切片厚度。 另外或替代地，齿阵列的齿相互间隔开不同的距离以有效地保持组织并限定规定的组织样本长度。

公开(公告)号：US20040098096A1

公开(公告)日：2004-05-20

申请号：US10680240

申请日：2003-10-08

申请人： The University of Miami

发明人： Darwin Eton

IPC分类号： A61F002/06

CPC分类号： A61F2/07 ,  A61F2/89 ,  A61F2002/065 ,  A61F2002/075 ,  A61F2002/077 ,  A61F2250/0003 ,  A61F2250/0018 ,  A61F2250/0024

摘要： An implantable endograft device which may be characterized as an endograft assembly that is effectively anchored with respect to the weakened blood vessel by filling the aneurysmal sac to preclude further enlargement thereof and to anchor the endograft with respect to the aneurysm. In this way, migration of the endograft is inhibited and exposure of the aneurysmal sac to endoleak circulatory pressures is limited thereby minimizing the risk of vessel wall rupture.

摘要翻译： 可植入的内移植物装置，其特征在于通过填充动脉瘤囊来相对于弱化的血管有效地锚定的内移植物组件，以阻止其进一步扩大并相对于动脉瘤锚定内移植物。 以这种方式，内移植物的迁移被抑制，并且动脉瘤囊暴露于内泄漏循环压力受到限制，从而使血管壁破裂的风险最小化。

公开(公告)号：US20020151052A1

公开(公告)日：2002-10-17

申请号：US09820809

申请日：2001-03-30

申请人： The University of Miami

发明人： Nirupa Chaudhari ,  Stephen D. Roper

IPC分类号： G01N033/53 ,  C07H021/04 ,  C07K014/705 ,  C12N005/06 ,  C12N005/08

CPC分类号： C07K14/705

摘要： A metabotropic glutamate taste receptor having a molecular weight of approximately 68 kDa, encoded by a cDNA sequence having the MRNA sequence of SEQ ID NO: 1 is disclosed, which functions as a umami taste receptor. Also disclosed are cells expressing the cloned receptor and a method of screening for umami mimics using the receptor.

摘要翻译： 公开了由具有SEQ ID NO：1的MRNA序列的cDNA序列编码的分子量约68kDa的代谢型谷氨酸味觉受体，其作为鲜味受体起作用。 还公开了表达克隆受体的细胞和使用该受体筛选鲜味模拟物的方法。

公开(公告)号：US6091977A

公开(公告)日：2000-07-18

申请号：US166335

申请日：1998-10-05

申请人： Peter P Tarjan ,  Monica Kaufer-Braverman ,  Leonard L. Rasquinha

发明人： Peter P Tarjan ,  Monica Kaufer-Braverman ,  Leonard L. Rasquinha

IPC分类号： A61F2/72 ,  A61B5/0492

CPC分类号： A61F2/72

摘要： A plurality of locally sensitive, far-field immune sensors are mounted in mutually spaced relation on the exterior of the body of a subject to detect electrical signals associated with voluntary skeletal muscle activity in the immediate physical vicinity of the sensors. The detected signals are used to determine which, if any, of a predetermined series of voluntary skeletal muscle maneuvers is performed by the subject. A signal bearing a predetermined relationship to the maneuver is then transmitted to a device to be controlled based on which maneuver the subject has performed. Also disclosed are a novel sensor and a method of making same.

摘要翻译： 多个局部敏感的远场免疫传感器以相互间隔的关系安装在受试者身体的外部上，以检测与传感器直接物理附近的自愿骨骼肌肉活动相关的电信号。 检测到的信号用于确定受试者执行预定系列的自愿骨骼肌肉动作中的哪一个（如果有的话）。 然后，基于对象进行的操纵，将与该操纵有预定关系的信号传送到要被控制的装置。 还公开了一种新颖的传感器及其制造方法。

公开(公告)号：US20210161934A1

公开(公告)日：2021-06-03

申请号：US17154192

申请日：2021-01-21

申请人： H. Lee Moffitt Cancer Center and Research Institute, Inc. ,  The University of Miami Leonard M. Miller School of Medicine

发明人： James Mulé ,  Rana Falahat ,  Glen Barber

IPC分类号： A61K31/7068 ,  A61K35/17 ,  A61K31/7084 ,  A61P35/00 ,  C12N5/0783 ,  G01N33/574

摘要： Disclosed herein is a method for enhancing antitumor T cell responses in subjects. The method involves administering to the subject in need thereof a composition comprising a demethylating agent in an amount effective to demethylate STING proteins in the tumor cells. This method is particularly useful in subjects with deficient STING expression in the tumor cells. Therefore, also disclosed is a method for treating a tumor in a subject that involves detecting in a biopsy sample from the subject reduced STING expression, reduced cGAS expression, or a combination thereof; and then administering to the subject a demethylating agent in an amount effective to demethylate STING proteins in the tumor cells. The method can further involve administering to the subject a therapeutically effective amount of a STING agonist. The method can further involve administering to the subject tumor infiltrating lymphocytes (TILs), such as HLA-matched TILs.

公开(公告)号：US09217696B2

公开(公告)日：2015-12-22

申请号：US14348304

申请日：2012-09-28

申请人： THE UNIVERSITY OF MIAMI

发明人： Azorides Morales

IPC分类号： A61B10/00 ,  G01N1/31 ,  G01N1/06 ,  G01N1/36 ,  G01N1/30

CPC分类号： G01N1/312 ,  G01N1/06 ,  G01N1/30 ,  G01N1/36

摘要： Improved methods and systems for processing of solid tissue are described. The method may be performed manually or automatically. The system may have modules such as (i) a grossing module where a fresh tissue is sliced to prepare a tissue specimen, (ii) a hardening module that hardens the tissue specimen, (iii) an impregnating module that impregnates the tissue specimen that was hardened, and (iv) an embedding module that embeds a tissue specimen that was hardened and impregnated. Fresh (i.e., not fixed or frozen) tissue, which was excised to diagnose disease or to assess surgical treatment, is grossed to about 0.6 mm. Preferably, the hardening of fresh tissue is initiated, but not completed, during grossing by contact with a chemical admixture. Preferably, dry ice, a thermoelectric device, or a gas condenser cools a metal mold containing the embedded specimen. It is sectioned and then microscopically examined as an alternative to histologic examination of a frozen section to avoid the known problems of discordant and deferred diagnosis.

摘要翻译： 描述了用于处理固体组织的改进的方法和系统。 该方法可以手动或自动执行。 该系统可以具有模块，例如（i）将新鲜组织切片以制备组织标本的总体模块，（ii）硬化组织样本的硬化模块，（iii）浸渍组织样本的浸渍模块， 硬化，以及（iv）嵌入模块，其嵌入被硬化和浸渍的组织样本。 被切除以诊断疾病或评估手术治疗的新鲜（即不固定或冷冻的）组织总共达到约0.6mm。 优选地，新鲜组织的硬化在通过与化学混合物接触的成本期间开始但未完成。 优选地，干冰，热电装置或气体冷凝器冷却包含嵌入样品的金属模具。 将其切片，然后进行显微镜检查，作为冷冻切片组织学检查的替代方法，以避免已知的不一致和延迟诊断问题。

公开(公告)号：US20150119557A1

公开(公告)日：2015-04-30

申请号：US14394346

申请日：2013-04-12

申请人： THE UNIVERSITY OF MIAMI ,  THE BRITISH COLUMBIA CANCER AGENCY BRANCH ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Andrew P. Weng ,  Jon C. Aster ,  Tan A. Ince

IPC分类号： C12N5/09 ,  C07K16/30 ,  G01N33/50

CPC分类号： C12N5/0694 ,  C07K16/28 ,  C07K16/2863 ,  C07K2317/73 ,  C07K2317/76 ,  C12N2500/92 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/125 ,  C12N2501/2302 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/42 ,  C12N2502/1394 ,  G01N33/5011 ,  G01N33/505 ,  G01N2500/10

摘要： We describe cell culture media for in vitro culture of a human cancer cell of the lymphocyte lineage (e.g., leukemia, lymphoma, or other blasts) or a precursor thereof, especially T-cell acute lymphoblastic leukemia lymphoma (T-ALL), as well as methods for at least maintenance, propagation, or both of the human cancer cell or its precursor.

摘要翻译： 我们描述了淋巴细胞谱系（例如白血病，淋巴瘤或其他原始细胞）或其前体，特别是T细胞急性成淋巴细胞白血病淋巴瘤（T-ALL）的人类癌细胞体外培养的细胞培养基，以及 作为用于至少维持，增殖或两者人癌细胞或其前体的方法。

公开(公告)号：US08252209B2

公开(公告)日：2012-08-28

申请号：US12805579

申请日：2010-08-06

申请人： Francisco M. Raymo ,  Massimiliano Tomasulo

发明人： Francisco M. Raymo ,  Massimiliano Tomasulo

IPC分类号： G02B5/23 ,  C07D265/12 ,  C07D265/00

CPC分类号： C07D498/04

摘要： We have designed a molecular switch based on the photoinduced opening and thermal closing of a [1,3]oxazine ring. A substituted [1,3]oxazine compound described as having a general (i.e., unsubstituted) structure with fused indoline and benzooxazine fragments such that they share a common bond in the [1,3]oxazine compound: (i) the bond connecting positions 1 and 2 of the indoline fragment and (ii) the bond connecting positions 2 and 3 of the benzooxazine fragment. Irradiation by light of suitable wavelength and intensity of this photochromic compound induces cleavage of a [C—O] bond of the [1,3]oxazine ring to form a phenolate chromophore. The photogenerated (e.g., colored) isomer may revert thermally to the starting (e.g., colorless) oxazine. Alternatively, the switch may be between isomers of the compound that absorb at different wavelengths. Reversible coloration of silica or polymeric materials and switching optical signals may involve many cycles of interconversion between different colored states. A colorless/colored state may be maintained by constant irradiation or chemical trapping.

摘要翻译： 我们设计了一种基于[1,3]恶嗪环的光致开放和热关闭的分子开关。 描述为具有稠合二氢吲哚和苯并恶嗪片段的通用（即未取代）结构的取代的[1,3]恶嗪化合物，使得它们在[1,3]恶嗪化合物中具有共同的键：（i）键连接位置 1和2的二氢吲哚片段和（ii）连接苯并恶嗪片段的2和3的键。 通过该光致变色化合物的适当波长和强度的光照射引起[1,3]恶嗪环的[C-O]键的切割以形成酚盐发色团。 光生的（例如着色的）异构体可以热回到起始（例如，无色）恶嗪。 或者，开关可以在吸收不同波长的化合物的异构体之间。 二氧化硅或聚合材料的可逆显色和切换光信号可能涉及不同着色状态之间的许多相互转换循环。 可以通过恒定的照射或化学捕获来维持无色/有色状态。