Evolution of bond-forming enzymes
    6.
    发明授权
    Evolution of bond-forming enzymes 有权
    键形成酶的进化

    公开(公告)号:US09267127B2

    公开(公告)日:2016-02-23

    申请号:US13922812

    申请日:2013-06-20

    IPC分类号: C12N9/52 C12P21/02 C12N9/64

    摘要: Strategies, systems, methods, reagents, and kits for the directed evolution of bond-forming enzymes are provided herein. Evolution products, for example, evolved sortases exhibiting enhanced reaction kinetics and/or altered substrate preferences are also provided herein, as are methods for using such evolved bond-forming enzymes. Kits comprising materials, reagents, and cells for carrying out the directed evolution methods described herein are also provided.

    摘要翻译: 本文提供了用于形成键的酶的定向进化的策略,系统,方法,试剂和试剂盒。 本文还提供了进化产物,例如展现出增强的反应动力学和/或改变的底物偏好的进化分类,以及使用这种进化的形成键的酶的方法。 还提供了包括用于实施本文所述的定向进化方法的材料,试剂和细胞的试剂盒。