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公开(公告)号:US20240229003A1
公开(公告)日:2024-07-11
申请号:US18425681
申请日:2024-01-29
发明人: Xinjian LIN , Xiying SHANG , Stephen B. HOWELL
CPC分类号: C12N9/6467 , A61K47/65 , A61K47/6889 , C07K14/43504 , C07K14/59 , C12N9/52 , C12N9/6424 , C12N9/96 , C12Y304/21079 , C12Y304/22007 , C12Y304/2207 , A61K48/00 , C07K2319/00 , C07K2319/21 , C07K2319/33 , C07K2319/40 , C07K2319/55 , C07K2319/70 , C07K2319/74 , C07K2319/90
摘要: The invention is directed to cell-targeted cytotoxic agents, including sortase serine protease constructs. Methods for targeted cell killing for treatment of proliferative diseases, for example, cancer, are provided. Exemplary embodiments comprise an R-spondin ligand for targeting the cytotoxic agents to effect the cell killing.
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公开(公告)号:US20240191276A1
公开(公告)日:2024-06-13
申请号:US18547468
申请日:2022-02-23
CPC分类号: C12P21/02 , C12N9/52 , C12N9/93 , C12Y304/2207 , C12Y601/01022
摘要: The present invention lies in the technical field of enzymatic (poly)peptide ligation and specifically relates to methods that allow the ligation of (poly)peptides and oligonucleotides. The methods comprise providing at least one cargo molecule modified with a peptide tag and at least one poly(peptide) to be ligated to the cargo molecule, wherein the peptide tag and/or the (poly)peptide comprises a ligation motif for a peptide ligase, preferably sortase and peptidyl asparaginyl ligases (PALs), such as butelase-1, VyPAL2 or OaAEPI b. The invention also relates to the resulting conjugates and the corresponding uses.
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公开(公告)号:US20180346899A1
公开(公告)日:2018-12-06
申请号:US16054382
申请日:2018-08-03
发明人: Giulia Pasqual , Gabriel Victora
IPC分类号: C12N9/52 , A01K67/027 , G01N33/50
CPC分类号: C12N9/52 , A01K67/027 , A01K67/0275 , A01K2217/072 , A01K2227/105 , A01K2267/03 , A01K2267/0387 , C07K2319/70 , C12Y304/2207 , G01N33/5008 , G01N33/5047 , G01N2333/70532 , G01N2333/70578
摘要: An sortase-mediated intercellular labeling method allowing for tracking ligand-receptor interaction both in vitro and in vivo; and uses thereof for tracking molecule interactions both in vitro and in vivo, identifying modulators of ligand-receptor interaction, identifying potential binding partners of a protein of interest, identifying B cells expressing high affinity B cell receptors to antigens, and identifying the antigen to which a T cell of interest binds.
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公开(公告)号:US20180216091A1
公开(公告)日:2018-08-02
申请号:US15933705
申请日:2018-03-23
发明人: Mara Boenitz-Dulat , Martin Schatte
CPC分类号: C12N9/52 , C07K2319/30 , C12P21/02 , C12Y304/2207
摘要: Herein is reported a polypeptide comprising the amino acid sequence of SEQ ID NO: 38 as sole Listeria monocytogenes derived polypeptide and its use in conjugating polypeptides.
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公开(公告)号:US09862779B2
公开(公告)日:2018-01-09
申请号:US14658078
申请日:2015-03-13
发明人: Mariel Beck , Georg Tiefenthaler
CPC分类号: C07K16/468 , C07K1/1075 , C07K16/065 , C07K16/2881 , C07K16/32 , C07K2317/31 , C07K2317/52 , C07K2317/55 , C07K2319/00 , C07K2319/01 , C07K2319/21 , C12N9/52 , C12Y304/2207
摘要: Herein is reported a method for producing a polypeptide comprising at least two polypeptide domains comprising the step of cultivating a cell comprising (a) a nucleic acid encoding a soluble S. aureus sortase A with a C-terminal endoplasmic reticulum retention signal, (b) a nucleic acid encoding a first polypeptide domain comprising at its C-terminus a sortase motif followed by an endoplasmic reticulum retention signal, and (c) a nucleic acid encoding a second polypeptide domain comprising at its N-terminus at least a diglycine, whereby the cell secretes the sortase A conjugate of the first polypeptide domain and the second polypeptide domain, thereby producing a polypeptide comprising at least two polypeptide domains.
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公开(公告)号:US09267127B2
公开(公告)日:2016-02-23
申请号:US13922812
申请日:2013-06-20
发明人: David R. Liu , Irwin Chen , Brent M. Dorr
CPC分类号: C12N15/1058 , C12N9/52 , C12N9/6472 , C12N15/1037 , C12P21/02 , C12Y304/2207
摘要: Strategies, systems, methods, reagents, and kits for the directed evolution of bond-forming enzymes are provided herein. Evolution products, for example, evolved sortases exhibiting enhanced reaction kinetics and/or altered substrate preferences are also provided herein, as are methods for using such evolved bond-forming enzymes. Kits comprising materials, reagents, and cells for carrying out the directed evolution methods described herein are also provided.
摘要翻译: 本文提供了用于形成键的酶的定向进化的策略,系统,方法,试剂和试剂盒。 本文还提供了进化产物,例如展现出增强的反应动力学和/或改变的底物偏好的进化分类,以及使用这种进化的形成键的酶的方法。 还提供了包括用于实施本文所述的定向进化方法的材料,试剂和细胞的试剂盒。
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公开(公告)号:US20230220110A1
公开(公告)日:2023-07-13
申请号:US17851989
申请日:2022-06-28
CPC分类号: C07K16/32 , C07K16/468 , C12Y304/2207 , C07K2317/31 , C07K2317/52 , C07K2317/55 , C07K2319/30
摘要: Herein is reported a method for producing a bispecific antibody comprising the step of incubating
(i) an antibody Fab fragment or a scFv antibody comprising within the 20 C-terminal amino acid residues the amino acid sequence LPX1TG (SEQ ID NO: 01),
(ii) a one-armed antibody comprising a full length antibody heavy chain, a full length antibody light chain, and an Fc-heavy chain,
whereby the full length antibody heavy chain and the full length antibody light chain are cognate antibody chains that thereof forms an antigen binding site,
whereby the full length antibody heavy chain and the Fc-heavy chain are covalently linked to each other via one or more disulfide bonds forming an antibody hinge region, and
whereby the Fc-heavy chain has an oligoglycine amino acid sequence at its N-terminus,
and
(iii) a Sortase A enzyme.-
公开(公告)号:US20180187217A1
公开(公告)日:2018-07-05
申请号:US15650852
申请日:2017-07-15
申请人: Rongzhen Zhang , Yan Xu , Kunpeng Li
发明人: Rongzhen Zhang , Yan Xu , Kunpeng Li
CPC分类号: C12P7/04 , C07B2200/07 , C07C29/143 , C07C33/26 , C07K2/00 , C12N9/0006 , C12N9/0008 , C12N9/52 , C12N15/70 , C12P7/22 , C12Q1/686 , C12Y304/2207
摘要: Disclosed are methods for the synthesis of chiral alcohols by Sortase A-mediated oxidoreductase oligomers, which relates to the field of biocatalysis. In the present disclosure, oxidoreductase oligomers were used as biocatalysts for chiral alcohol preparation. Compared to wild-type enzymes, the oxidoreductase oligomers significantly improved catalytic activity and thermal stability. The sortase A-mediated oxidoreductase oligomers had 6-8 folds improvement in specific activity over that of the wild-type enzymes. The oligomers displayed a Tm value 6-12° C. higher than that of the wild-type, suggesting the sortase A-mediated oxidoreductase oligomers significantly improved thermostability of the enzymes. The oxidoreductase oligomers catalyzed asymmetric transformation to produce (S)-1-phenyl-1,2-ethanediol or (R)-1-phenethyl alcohol within 3-6 hr, with an optical purity of 98%-100% and a yield of 98%-99%.
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公开(公告)号:US20170340754A1
公开(公告)日:2017-11-30
申请号:US15523325
申请日:2015-10-29
发明人: Zheng-Yi Chen , David Liu
IPC分类号: A61K48/00 , C07J7/00 , C07K7/06 , C12N9/22 , C07K7/08 , C12N15/11 , C12N9/52 , C07K19/00 , C07K14/47 , C07K14/46 , A61K9/127 , C12N15/113 , C12N2310/20
CPC分类号: A61K48/0008 , A61K9/1272 , A61K38/00 , A61K38/10 , A61K47/64 , A61K47/6911 , A61K48/0075 , A61K48/0083 , C07J7/009 , C07K7/06 , C07K7/08 , C07K14/463 , C07K14/47 , C07K19/00 , C07K2319/06 , C07K2319/21 , C07K2319/33 , C07K2319/60 , C12N5/062 , C12N9/00 , C12N9/22 , C12N9/52 , C12N15/11 , C12N15/113 , C12N15/87 , C12N15/88 , C12N15/907 , C12N2310/20 , C12N2310/3513 , C12N2510/00 , C12Y304/2207
摘要: Compositions are described for direct protein delivery into multiple cell types in the mammalian inner ear. The compositions are used to deliver protein(s) (such as gene editing factors) editing of genetic mutations associated with deafness or associated disorders thereof. The delivery of genome editing proteins for gene editing and correction of genetic mutations protect or restore hearing from genetic deafness. Methods of treatment include the intracellular delivery of these molecules to a specific therapeutic target.
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公开(公告)号:US20170260517A1
公开(公告)日:2017-09-14
申请号:US15434648
申请日:2017-02-16
发明人: Xinjian LIN , Xiying SHANG , Stephen B. HOWELL
IPC分类号: C12N9/64 , C07K14/435 , C07K14/59 , C12N9/96
CPC分类号: C12N9/6467 , A61K47/65 , A61K47/6889 , A61K48/00 , C07K14/43504 , C07K14/59 , C07K2319/00 , C07K2319/21 , C07K2319/33 , C07K2319/40 , C07K2319/55 , C07K2319/70 , C07K2319/74 , C07K2319/90 , C12N9/52 , C12N9/6424 , C12N9/96 , C12Y304/21079 , C12Y304/22007 , C12Y304/2207
摘要: Cell-targeted cytotoxic agents, including sortase serine protease constructs, are provided. Such compounds can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant sortase serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity.
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