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    Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
    2.
    发明授权
    Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations 失效
    同时鉴别差异表达mRNA的方法和相对浓度的测定

    公开(公告)号：US06633818B1

    公开(公告)日：2003-10-14

    申请号：US09630202

    申请日：2000-08-01

    Applicant: J. Gregor Sutcliffe ,  Karl W. Hasel

    Inventor： J. Gregor Sutcliffe ,  Karl W. Hasel

    IPC: G01N3348

    CPC classification number: C12Q1/6809 ,  C12N15/1096 ,  C12Q1/6813 ,  C12Q1/6853 ,  C12Q2525/185 ,  C12Q2539/113 ,  C12Q2525/131 ,  C12Q2525/173

    Abstract: An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3′-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of 5′-RT primers, and performing PCR using a 3′-PCR primer whose sequence is derived from the vector and a set of 5′-PCR primers that is derived from the 5′-RT primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.

    Abstract translation: 用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。 通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组5'-RT引物从cRNA转录cDNA，并使用序列衍生自载体的3'-PCR引物进行PCR，以及从5'端衍生的一组5'-PCR引物 -RT引物用于从cRNA转录cDNA。 该方法可以鉴定与给药药物或与生理或病理状况相关的mRNA的表达变化

    Breast Specific Genes and Proteins
    4.
    发明申请
    Breast Specific Genes and Proteins 审中-公开
    乳腺特异性基因和蛋白质

    公开(公告)号：US20060257409A1

    公开(公告)日：2006-11-16

    申请号：US11383080

    申请日：2006-05-12

    Applicant: Hongjun Ji ,  Craig Rosen

    Inventor： Hongjun Ji ,  Craig Rosen

    IPC: A61K48/00 ,  C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  A61K39/395 ,  C07K14/82 ,  C07K16/30

    CPC classification number: C12Q1/6886 ,  A61K39/00 ,  C07K14/47 ,  C12Q1/6809 ,  C12Q2600/156 ,  G01N33/57415 ,  G01N2500/04 ,  C12Q2539/113

    Abstract: Human breast specific gene polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polynucleotides or polypeptides as a diagnostic marker for breast cancer and as an agent to determine if breast cancer has metastasized. Also disclosed are antibodies specific to the breast specific gene polypeptides which may be used to target cancer cells and be used as part of a breast cancer vaccine. Methods of screening for antagonists for the polypeptide and therapeutic uses thereof are also disclosed.

    Abstract translation: 公开了人类乳腺特异性基因多肽和编码此类多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这样的多核苷酸或多肽作为乳腺癌的诊断标记物和用作确定乳腺癌是否转移的药剂的方法。 还公开了可用于靶向癌细胞并用作乳腺癌疫苗的一部分的乳腺特异性基因多肽特异性抗体。 还公开了筛选多肽的拮抗剂的方法及其治疗用途。

    LOCALISED RCA-BASED AMPLIFICATION METHOD USING A PADLOCK-PROBE
    7.
    发明申请
    LOCALISED RCA-BASED AMPLIFICATION METHOD USING A PADLOCK-PROBE 审中-公开

    公开(公告)号：US20160289750A1

    公开(公告)日：2016-10-06

    申请号：US15036394

    申请日：2014-11-14

    Applicant: OLINK AB

    Inventor： Ulf LANDEGREN ,  Lei CHEN

    IPC: C12Q1/68

    CPC classification number: C12Q1/6844 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6853 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q2531/125 ,  C12Q2563/179 ,  C12Q2531/143 ,  C12Q2537/125 ,  C12Q2531/119 ,  C12Q2537/143 ,  C12Q2539/113 ,  C12Q2525/161 ,  C12Q2565/102 ,  C12Q2525/307 ,  C12Q2563/131

    Abstract: The present invention provides a method for performing a localised RCA reaction comprising at least two rounds of RCA, wherein the product of a second RCA reaction is attached, and hence localised, to a product of a first RCA reaction, said method comprising: (a) providing a concatemeric first RCA product comprising repeated monomers; (b) directly or indirectly hybridising to monomers of said first RCA product a circularisable oligonucleotide comprising target-complementary 3′ and 5′ end regions such that the 3′ and 5′ ends of said oligonucleotide hybridise in juxtaposition for ligation directly or indirectly to each other, wherein the target is a sequence in a monomer of said first RCA product or an intermediate molecule hybridised thereto, and wherein the target-complementary end regions of said circularisable oligonucleotide are 6 to 16 nucleotides in length; (c) directly or indirectly ligating the ends of said circularisable oligonucleotide to circularise the oligonucleotide, thereby to provide a template for a second RCA reaction, wherein when said ends are indirectly ligated (i) either a gap oligonucleotide is provided which hybridises to the monomers of the first RCA product in between the 3′ and 5′ ends of the circularisable oligonucleotide such that it may be ligated to the respective ends, or the hybridised 3′ end of the circularisable oligonucleotide is extended by a polymerase such that the extended 3′ end may be ligated to the hybridised 5′ end, and (ii) the total length of the region of the second RCA template directly or indirectly hybridised to the monomers is no longer than 32 nucleotides in length; and (d) performing a second RCA reaction using said second RCA template of (c) and a primer for said second RCA, to form a second RCA product, wherein in said second RCA reaction the second RCA template remains attached to the first RCA product, and thereby the second RCA product is attached to the first RCA product.

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