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    POLY-3-HYDROXYALKANOATES HAVING VINYL MOIETIES AND METHOD OF PRODUCING SUCH
    2.
    发明公开
    POLY-3-HYDROXYALKANOATES HAVING VINYL MOIETIES AND METHOD OF PRODUCING SUCH 审中-公开

    公开(公告)号：US20240060099A1

    公开(公告)日：2024-02-22

    申请号：US18030970

    申请日：2021-10-11

    申请人： POLYFERM CANADA INC.

    发明人： Bruce RAMSAY ,  Juliana RAMSAY ,  Araceli FLORES-SANCHEZ

    IPC分类号： C12P7/625 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N15/74

    CPC分类号： C12P7/625 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/74 ,  C12N2500/30 ,  C12Y103/08001 ,  C12Y402/01017

    摘要： A method of producing polyhydroxyalkanoate (PHA) copolymers comprising both short-chain-length (scl) and medium-chain-length (mcl) subunits, wherein some of the mcl subunits bear reactive vinyl groups, is provided. The method comprises providing cells comprising (i) a PHA synthase (phaC) gene encoding a particular class I poly(3-hydroxyalkanoate) polymerase, and (ii) a phaJ gene encoding a particular (R)-specific enoyl-CoA hydratase, and cultivating said cells in a growth medium comprising an alkenoic acid. In one example, cells grown in the presence of a mixture of decanoic acid and undecen-10-enoic acid yielded a copolymer comprising units of 3-hydroxybutyrate (94.73 mol %), 3-hydroxyhexanoate (1.51 mol %), 3-hydroxyoctanoate (2.33 mol %), 3-hydroxydecanoate (0.69 mol %) and 3-hydroxyhept-6-enoate (0.73 mol %). The presence of acrylic acid as a 13-oxidation inhibitor in the growth medium led to an increase in both the mcl-PHA subunit content and vinyl subunit content. For example, a copolymer containing about 1.2 mol % 3-hydroxyhept-6-enoate and about 0.5 mol % 3-hydroxynon-8-enoate was obtained at a concentration of 40 mM acrylic acid. PHA accumulation of up to 57.2% (w/w of cell dry weight) are reported. The scl-mcl PHAs with subunits bearing reactive vinyl groups are expected to be useful in a variety of applications, for instance for covalently linking bioactive molecules.