公开(公告)号：US20240218403A1

公开(公告)日：2024-07-04

申请号：US18285138

申请日：2022-04-01

申请人： Ginkgo Bioworks, Inc.

发明人： Guillaume Beaudoin ,  Elena Brevnova ,  Alkiviadis Orfefs Chatzivasileiou ,  Alexandra Exner ,  Annapuma Kamineni ,  Matthew McMahon ,  Joshua Trueheart

IPC分类号： C12P7/42 ,  C12N1/16 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12P17/02 ,  C12P33/00 ,  C12R1/19 ,  C12R1/865

CPC分类号： C12P7/42 ,  C12N1/165 ,  C12N1/205 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12P17/02 ,  C12P33/00 ,  C12R2001/19 ,  C12R2001/865 ,  C12Y101/01034 ,  C12Y101/01088 ,  C12Y203/01009 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y207/04026 ,  C12Y401/01033 ,  C12Y503/03002 ,  C12Y504/99007

摘要： Described in this application are proteins and host cells involved in methods of producing isoprenoid precursors and/or isoprenoids.

公开(公告)号：US20230265465A1

公开(公告)日：2023-08-24

申请号：US17962229

申请日：2022-10-07

申请人： Khona Scientific Holdings, Inc.

发明人： Jordan Buck

IPC分类号： C12P7/42 ,  C12N15/82 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81

CPC分类号： C12P7/42 ,  C12N15/8222 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  C12N2330/51 ,  C12Y203/01009 ,  C12Y121/03008 ,  C12Y103/03 ,  C12Y121/03007 ,  C12Y203/01206 ,  C12Y404/01026 ,  C12Y205/01001 ,  C12Y503/03002 ,  C12Y101/01157 ,  C12Y402/01017 ,  C12Y203/01016 ,  C12Y103/01038 ,  C12Y203/0301 ,  C12Y101/01034 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y203/03008 ,  C12Y604/01002 ,  C12N2800/40

摘要： This document relates to using bidirectional, multi-enzymatic scaffolds to biosynthesize cannabinoids in recombinant hosts.

公开(公告)号：US20170009240A1

公开(公告)日：2017-01-12

申请号：US14950425

申请日：2015-11-24

申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Han Min WOO ,  Eun Mi KIM ,  Youngsoon UM ,  Gyeongtaek GONG ,  Sun Mi LEE ,  Yunje KIM

IPC分类号： C12N15/52 ,  C12N15/71 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04 ,  C12N9/12 ,  C12N15/72 ,  C12N9/10

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/71 ,  C12N15/72 ,  C12Y101/01034 ,  C12Y203/01026 ,  C12Y205/01 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y401/03 ,  C12Y503/03002

摘要： Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

摘要翻译： 本文公开了能够表达月桂烯的表达载体，用载体转化的大肠杆菌菌株，并且具有改进的生产月桂烯的能力和用于制备月桂烯的方法和使用该方法回收甘油的方法。 在一方面，本公开的转化的大肠杆菌菌株可以使用甘油或葡萄糖作为碳源大量生产高纯度的月桂烯。 此外，本公开的大肠杆菌菌株是经济和环境友好的，因为它可以使用废甘油作为碳源产生高附加值的月桂烯。 此外，强烈挥发性月桂烯可以同时生产和分离。

公开(公告)号：US06989257B2

公开(公告)日：2006-01-24

申请号：US10166225

申请日：2002-06-05

申请人： Alan Berry ,  Werner Bretzel ,  Markus Hümbelin ,  Rual Lopez-Ulibarri ,  Anne Françoise Mayer ,  Alexei A. Yeliseev

发明人： Alan Berry ,  Werner Bretzel ,  Markus Hümbelin ,  Rual Lopez-Ulibarri ,  Anne Françoise Mayer ,  Alexei A. Yeliseev

IPC分类号： C12N9/02 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12N9/1022 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12P23/00 ,  C12R1/01 ,  C12Y101/01034 ,  C12Y101/01036 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033

摘要： Isolated polynucleotides encoding polypeptides having the activity of enzymes in the mevalonate pathway are provided. These sequences are useful for recombinantly producing isoprenoid compounds, such as carotenoids, in particular zeaxanthin. Expression vectors, cultured cells, and methods of making isoprenoid compounds are also provided.

公开(公告)号：US11993635B2

公开(公告)日：2024-05-28

申请号：US17816937

申请日：2022-08-02

申请人： The Regents of the University of Colorado, a Body Corporate

发明人： Jerome Fox ,  Ankur Sarkar ,  Akarawin Hongdusit ,  Edward Kim

IPC分类号： C12N1/20 ,  C07K14/005 ,  C07K14/47

CPC分类号： C07K14/005 ,  C07K14/47 ,  C12N1/20 ,  C07K2319/80 ,  C12Y205/01001 ,  C12Y205/0101 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y301/01048 ,  C12Y401/01033 ,  C12Y402/03017 ,  C12Y402/03018 ,  C12Y402/03024 ,  C12Y402/03056 ,  C12Y503/03002

摘要： This invention relates to the field of genetic engineering. Specifically, the invention relates to the construction of operons to produce biologically active agents. For example, operons may be constructed to produce agents that control the function of biochemical pathway proteins (e.g., protein phosphatases, kinases and/or proteases). Such agents may include inhibitors and modulators that may be used in studying or controlling phosphatase function associated with abnormalities in a phosphatase pathway or expression level. Fusion proteins, such as light activated protein phosphatases, may be genetically encoded and expressed as photoswitchable phosphatases. Systems are provided for use in controlling phosphatase function within living cells or in identifying small molecule inhibitors/activator/modulator molecules of protein phosphatases associated with cell signaling.

公开(公告)号：US20230340506A1

公开(公告)日：2023-10-26

申请号：US18054917

申请日：2022-11-14

申请人： The Regents of the University of California

发明人： Jay D. KEASLING ,  Leo D′ESPAUX ,  Jeff WONG ,  Xiaozhou LUO ,  Michael REITER ,  Charles DENBY ,  Anna LECHNER

IPC分类号： C12N15/81 ,  C12N9/10 ,  C12N15/70 ,  C12P7/42 ,  C07C63/04 ,  C12N15/82

CPC分类号： C12N15/81 ,  C07C63/04 ,  C12N9/1085 ,  C12N15/70 ,  C12N15/8243 ,  C12P7/42 ,  C12N15/52 ,  C12Y101/01088 ,  C12Y203/01086 ,  C12Y203/01206 ,  C12Y203/0301 ,  C12Y205/01102 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y503/03002 ,  C12Y602/01003 ,  C12Y604/01002

摘要： The present disclosure provides genetically modified host cells that produce a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative. The present disclosure provides methods of synthesizing a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative.

公开(公告)号：US11674184B2

公开(公告)日：2023-06-13

申请号：US16766297

申请日：2018-11-21

申请人： ENHANCEDBIO INC.

发明人： Eun Kyung Choi ,  Seong-Yun Jeong ,  Si Yeol Song ,  Yun-Yong Park ,  Seok Soon Park ,  Eun Jin Ju ,  Eun Jung Ko

IPC分类号： A61K38/55 ,  A61K31/7105 ,  A61P35/00 ,  C12N15/113 ,  C12Q1/6886 ,  A61K38/00

CPC分类号： C12Q1/6886 ,  A61K31/7105 ,  A61K38/005 ,  A61K38/55 ,  A61P35/00 ,  C12N15/1137 ,  C12N2310/14 ,  C12Y207/04002

摘要： The present invention relates to a biomarker composition for diagnosing radiation-resistant cancer comprising PMVK as an active ingredient and a method of diagnosing radiation-resistant cancer using the same, and when the PMVK is knocked down, it is confirmed that the survival rate of cancer cells decreases during radiation treatment, and based on this, the possibility as a factor related to radiation therapy resistance to cancer was suggested, and the PMVK can be used as a new target to enhance the effect of radiation therapy on human cancer cells.

公开(公告)号：US20170145441A1

公开(公告)日：2017-05-25

申请号：US15238225

申请日：2016-08-16

申请人： INVISTA North America S.à.r.l.

发明人： Alex Van Eck CONRADIE

IPC分类号： C12P5/00 ,  C12N9/10 ,  C12N9/04 ,  C12P7/42 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P9/00 ,  C12N15/74 ,  C12N9/12

CPC分类号： C12P5/007 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12P7/42 ,  C12P9/00 ,  C12Y101/01034 ,  C12Y101/01088 ,  C12Y203/01009 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y402/03027 ,  C12Y503/03002

摘要： This application describes methods, including non-naturally occurring methods, for biosynthesizing unsaturated pentahydrocarbons, such as isoprene and intermediates thereof, via the mevalonate pathway, as well as non-naturally occurring hosts for producing isoprene.

公开(公告)号：US20240199704A1

公开(公告)日：2024-06-20

申请号：US18443086

申请日：2024-02-15

申请人： The Regents of the University of Colorado, a Body Corporate

发明人： Jerome FOX ,  Ankur SARKAR ,  Akarawin HONGDUSIT ,  Edward KIM

IPC分类号： C07K14/005 ,  C07K14/47 ,  C12N1/20

CPC分类号： C07K14/005 ,  C07K14/47 ,  C12N1/20 ,  C07K2319/80 ,  C12Y205/01001 ,  C12Y205/0101 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y301/01048 ,  C12Y401/01033 ,  C12Y402/03017 ,  C12Y402/03018 ,  C12Y402/03024 ,  C12Y402/03056 ,  C12Y503/03002

摘要： This invention relates to the field of genetic engineering. Specifically, the invention relates to the construction of operons to produce biologically active agents. For example, operons may be constructed to produce agents that control the function of biochemical pathway proteins (e.g., protein phosphatases, kinases and/or proteases). Such agents may include inhibitors and modulators that may be used in studying or controlling phosphatase function associated with abnormalities in a phosphatase pathway or expression level. Fusion proteins, such as light activated protein phosphatases, may be genetically encoded and expressed as photoswitchable phosphatases. Systems are provided for use in controlling phosphatase function within living cells or in identifying small molecule inhibitors/activator/modulator molecules of protein phosphatases associated with cell signaling.

公开(公告)号：US11746344B2

公开(公告)日：2023-09-05

申请号：US16073073

申请日：2017-01-26

申请人： INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY

发明人： Seon Won Kim ,  Jung Hun Kim ,  Myeong Seok Cha ,  Hui Jeong Jang

IPC分类号： C12N9/88 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12P5/00

CPC分类号： C12N9/88 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/90 ,  C12P5/007 ,  C12Y203/01194 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y402/03027

摘要： A method for producing isoprene includes culturing E. coli, which has isoprene productivity and in which a gene encoding a recA protein is attenuated or deleted, in a medium containing a carbon source. Therefore, a great amount of isoprene may be produced within a short period of time, and thereby considerably decreasing isoprene production unit costs.