公开(公告)号：US11845973B2

公开(公告)日：2023-12-19

申请号：US17679007

申请日：2022-02-23

申请人： LG CHEM, LTD.

发明人： Hye Ok Kang ,  Donggyun Kang ,  Chul Woong Kim ,  In Young Huh ,  Jung Yun Choi

IPC分类号： C08G63/06 ,  C12P7/625 ,  C12N1/20 ,  C12N15/70 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C08G63/08 ,  C12N9/88

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/08 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12N15/70 ,  C12Y101/01027 ,  C12Y101/01028 ,  C12Y102/01003 ,  C12Y203/01 ,  C12Y208/03001 ,  C12Y402/0103 ,  C12Y402/01036 ,  C12N2510/02

摘要： The present invention relates to a novel 3-hydroxypropionate-lactate block copolymer [P(3HP-b-LA)], and a method for preparing same, and more specifically, provides a method for preparing a 3-hydroxypropionate-lactate block copolymer, and a 3-hydroxypropionate-lactate block copolymer produced thereby, the method comprising: a first culture step in which, by using recombinant E. coli improved so as to be incapable of biosynthesizing lactic acid, P(3HP) is biosynthesized at the early stage of culturing by having glycerol as a carbon source and through 3-hydroxypropionate-generating genes and an enhanced PHA synthase; and a second culture step in which P(3HP) production is inhibited by using a carbon catabolic repression system for selectively introducing only glucose into E. coli when glycerol and glucose are supplied together as carbon sources, and in which polylactate is biosynthesized to an interrupted P(3HP) terminus by the enabling of the expression of a lactate synthase and a lactyl-CoA converting enzyme through an IPTG induction system.

公开(公告)号：US20230407314A1

公开(公告)日：2023-12-21

申请号：US18035068

申请日：2021-11-02

申请人： Ambareesh Govind Phadnavis

发明人： Ambareesh Govind Phadnavis

IPC分类号： C12N15/52 ,  C12P7/6409 ,  C12P7/6445 ,  C12P13/08 ,  C12P7/06 ,  C12N9/88 ,  C12N9/10 ,  C12N9/04 ,  C12N9/12 ,  C12N9/16 ,  C12N9/00

CPC分类号： C12N15/52 ,  C12P7/6409 ,  C12P7/6445 ,  C12P13/08 ,  C12P7/06 ,  C12N9/88 ,  C12Y401/01032 ,  C12Y402/01036 ,  C12N9/1096 ,  C12Y206/01001 ,  C12N9/1025 ,  C12Y203/03001 ,  C12N9/0006 ,  C12Y101/01087 ,  C12Y101/01008 ,  C12Y101/01042 ,  C12Y101/09001 ,  C12Y401/02048 ,  C12N9/1029 ,  C12Y203/01054 ,  C12Y206/01042 ,  C12N9/1205 ,  C12Y207/01086 ,  C12N9/16 ,  C12Y301/02014 ,  C12N9/93 ,  C12Y604/01002

摘要： The invention provides a genetically modified eukaryotic microorganism for anaerobic production of essential amino acids and optionally the co-production of one or more co-products. The microorganism is genetically modified to redirect carbon flow from PEP via oxaloacetate and asparatate semialdehyde, towards the synthesis of increased amounts of essential amino acids. The microorganism may be genetically modified to produce increased amounts of one or more co-product by enhancing carbon flow from PEP via pyruvate, acetyl CoA and malonyl CoA to produce alcohols and lipids, such as triglycerides, fatty esters, fatty alcohols, fatty aldehydes, fatty amides. The invention provides a method for anaerobic production of essential amino acids using the genetically modified eukaryotic microorganism and optionally co-production of said one or more co-products. The genetically modified eukaryotic microorganism may be used for the anaerobic production of essential amino acids and optionally the co-production of said one or more co-products.

公开(公告)号：US09580731B2

公开(公告)日：2017-02-28

申请号：US14138971

申请日：2013-12-23

申请人： INVISTA North America S.á r.l.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie ,  Changlin Chen ,  Paul S. Pearlman

IPC分类号： C12P13/00 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12N15/52

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01 ,  C12Y101/01085 ,  C12Y101/01087 ,  C12Y101/01258 ,  C12Y101/01286 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y102/0101 ,  C12Y102/99006 ,  C12Y203/01032 ,  C12Y203/03013 ,  C12Y203/03014 ,  C12Y206/01 ,  C12Y207/08 ,  C12Y208/03012 ,  C12Y301/02 ,  C12Y305/01062 ,  C12Y401/01043 ,  C12Y402/01033 ,  C12Y402/01036 ,  C12Y402/01114 ,  C12Y602/01005

摘要： This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.

摘要翻译： 本文件描述了通过在C7脂肪族中形成一个或两个由羧基，胺或羟基组成的末端官能团，生成庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇的生化途径 骨架底物。 本文描述的这些途径，代谢工程和培养策略依赖于C1延长酶或与辅酶B生物合成相关的同源物。

公开(公告)号：US20170204420A1

公开(公告)日：2017-07-20

申请号：US15405514

申请日：2017-01-13

申请人： INVISTA North American S.á r.I.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie ,  Changlin Chen ,  Paul S. Pearlman

IPC分类号： C12N15/52 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12P7/24 ,  C12N9/02 ,  C12N9/00 ,  C12N9/80 ,  C12P7/44 ,  C12P13/00 ,  C12N9/04 ,  C12N9/16

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01 ,  C12Y101/01085 ,  C12Y101/01087 ,  C12Y101/01258 ,  C12Y101/01286 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y102/0101 ,  C12Y102/99006 ,  C12Y203/01032 ,  C12Y203/03013 ,  C12Y203/03014 ,  C12Y206/01 ,  C12Y207/08 ,  C12Y208/03012 ,  C12Y301/02 ,  C12Y305/01062 ,  C12Y401/01043 ,  C12Y402/01033 ,  C12Y402/01036 ,  C12Y402/01114 ,  C12Y602/01005

摘要： This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.

公开(公告)号：US20140193863A1

公开(公告)日：2014-07-10

申请号：US14138971

申请日：2013-12-23

申请人： INVISTA North America S.á r.I.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie ,  Changlin Chen ,  Paul S. Pearlman

IPC分类号： C12P13/00 ,  C12P7/42 ,  C12P7/18 ,  C12P7/44

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01 ,  C12Y101/01085 ,  C12Y101/01087 ,  C12Y101/01258 ,  C12Y101/01286 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y102/0101 ,  C12Y102/99006 ,  C12Y203/01032 ,  C12Y203/03013 ,  C12Y203/03014 ,  C12Y206/01 ,  C12Y207/08 ,  C12Y208/03012 ,  C12Y301/02 ,  C12Y305/01062 ,  C12Y401/01043 ,  C12Y402/01033 ,  C12Y402/01036 ,  C12Y402/01114 ,  C12Y602/01005

摘要： This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.

摘要翻译： 本文件描述了通过在C7脂肪族中形成一个或两个由羧基，胺或羟基组成的末端官能团，生成庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇的生化途径 骨架底物。 本文描述的这些途径，代谢工程和培养策略依赖于C1延长酶或与辅酶B生物合成相关的同源物。