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    Actinobacillus succinogenes shuttle vector and methods of use
    2.
    发明申请
    Actinobacillus succinogenes shuttle vector and methods of use 有权

    公开(公告)号：US20050164347A1

    公开(公告)日：2005-07-28

    申请号：US11042977

    申请日：2005-01-25

    申请人： J. Zeikus ,  Maris Laivenieks ,  Claire Vieille ,  Pil Kim

    发明人： J. Zeikus ,  Maris Laivenieks ,  Claire Vieille ,  Pil Kim

    IPC分类号： C12N9/88 ,  C12N15/74 ,  C12P7/46 ,  C07K14/195 ,  C07H21/04 ,  C12N1/21

    CPC分类号： C12N15/74 ,  C12N9/88 ,  C12P7/46 ,  C12Y401/01032

    摘要： An Actinobacillus succinogenes plasmid vector which provides a means to overexpress proteins in A. succinogenes. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner in A. succinogenes. The plasmid comprises at least one marker gene, operably linked to a first promoter functional in Actinobacillus succinogenes, an origin of replication functional in Actinobacillus succinogenes, a second promoter isolated from Actinobacillus succinogenes, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence of Actinobacillus succinogenes, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinant Actinobacillus succinogenes is described, including a method of transformation. Additionally, a recombinant Actinobacillus succinogenes is disclosed and a method for producing succinate utilizing this recombinant Actinobacillus succinogenes is described.

    Actinobacillus succinogenes shuttle vector and methods of use
    5.
    发明授权
    Actinobacillus succinogenes shuttle vector and methods of use 有权

    公开(公告)号：US07192761B2

    公开(公告)日：2007-03-20

    申请号：US11042407

    申请日：2005-01-25

    申请人： J. Gregory Zeikus ,  Maris Laivenieks ,  Claire Vieille ,  Pil Kim

    发明人： J. Gregory Zeikus ,  Maris Laivenieks ,  Claire Vieille ,  Pil Kim

    IPC分类号： C12N1/21 ,  C12N15/74 ,  C12N15/11 ,  C07H21/04

    CPC分类号： C12N15/74 ,  C12N9/88 ,  C12P7/46 ,  C12Y401/01032

    摘要： An Actinobacillus succinogenes plasmid vector which provides a means to overexpress proteins in A. succinogenes. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner in A. succinogenes. The plasmid comprises at least one marker gene, operably linked to a first promoter functional in Actinobacillus succinogenes, an origin of replication functional in Actinobacillus succinogenes, a second promoter isolated from Actinobacillus succinogenes, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence of Actinobacillus succinogenes, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinant Actinobacillus succinogenes is described, including a method of transformation. Additionally, a recombinant Actinobacillus succinogenes is disclosed and a method for producing succinate utilizing this recombinant Actinobacillus succinogenes is described.

    METABOLIC EVOLUTION OF ESCHERICHIA COLI STRAINS THAT PRODUCE ORGANIC ACIDS
    7.
    发明申请
    METABOLIC EVOLUTION OF ESCHERICHIA COLI STRAINS THAT PRODUCE ORGANIC ACIDS 审中-公开
    生产有机酸的食品菌株的代谢演化

    公开(公告)号：US20160083753A1

    公开(公告)日：2016-03-24

    申请号：US14491073

    申请日：2014-09-19

    申请人： MYRIANT CORPORATION

    发明人： Tammy Grabar ,  Wei Gong ,  R. Rogers Yocum

    IPC分类号： C12P7/46 ,  C12N9/88 ,  C12N9/00

    CPC分类号： C12P7/46 ,  C12N9/88 ,  C12N9/93 ,  C12P2203/00 ,  C12Y207/03009 ,  C12Y401/01031 ,  C12Y401/01032 ,  C12Y604/01001

    摘要： This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

    摘要翻译： 本发明涉及先前优化用于在发酵条件下以商业上显着的量生产有机酸的微生物生物的代谢进化，其中使用己糖作为最小矿物培养基中的唯一碳源。 作为这种代谢进化的结果，微生物生物体获得使用来自纤维素材料的戊糖的生长能力，同时保持原始生长动力学，有机酸生产速率和使用己糖作为碳源的能力 。 本发明还公开了赋予在生产商业上大量有机酸中同时使用己糖和戊糖的能力的微生物中的遗传变化。

    MODULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE-MITCHONDRIAL (PEPCK-M) EXPRESSION
    9.
    发明申请
    MODULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE-MITCHONDRIAL (PEPCK-M) EXPRESSION 审中-公开
    磷酸羟丙酯羧酸酶（PEPCK-M）表达的调节

    公开(公告)号：US20130217749A1

    公开(公告)日：2013-08-22

    申请号：US13702965

    申请日：2011-06-10

    申请人： Sanjay Bhanot ,  Gerald Shulman

    发明人： Sanjay Bhanot ,  Gerald Shulman

    IPC分类号： A61K31/7088

    CPC分类号： A61K31/7088 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12Y401/01032 ,  C12N2310/321 ,  C12N2310/3525

    摘要： Provided herein are methods, compounds, and compositions for reducing expression of phosphoenolpyruvate carboxykinase-mitochondrial (PEPCK-M) mRNA and protein in an animal. Also provided herein are methods, compounds, and compositions for preventing or decreasing diabetes, obesity, metabolic syndrome, diabetic dyslipidemia, and/or hypertriglyceridemia in an animal. Such methods, compounds, and compositions are useful to treat, prevent, delay, or ameliorate any one or more of diabetes, obesity, metabolic syndrome, diabetic dyslipidemia, and/or hypertriglyceridemia, or a symptom thereof.

    摘要翻译： 本文提供了用于在动物中降低磷酸烯醇丙酮酸羧激酶 - 线粒体（PEPCK-M）mRNA和蛋白质的表达的方法，化合物和组合物。 本文还提供用于预防或减少动物体内的糖尿病，肥胖，代谢综合征，糖尿病性血脂异常和/或高甘油三酯血症的方法，化合物和组合物。 这些方法，化合物和组合物可用于治疗，预防，延迟或改善糖尿病，肥胖，代谢综合征，糖尿病性血脂异常和/或高甘油三酯血症或其症状中的任何一种或多种。