Device and method for multispot scanning microscopy

    公开(公告)号:US20170227748A1

    公开(公告)日:2017-08-10

    申请号:US15427337

    申请日:2017-02-08

    IPC分类号: G02B21/00 G02F1/11

    摘要: The invention relates to a device for multispot scanning microscopy, having a multicolour light source for providing at least one illumination light beam, having a splitting device for splitting the illumination light beam into a plurality of illumination sub-beams, having first optical means for providing an illumination optical path for guiding and focussing the individual illumination sub-beams respectively into a light spot on or in a specimen to be examined, having a scan unit for guiding the light spots over the probe, having a detection unit for detecting detection light emitted by the specimen in detection sub-beams after irradiation with the individual illumination sub-beams, having second optical means for providing a detection optical path for guiding the detection sub-beams to the detector unit, having a control and evaluation unit for controlling the scan unit and for evaluating the detection light detected by the detection unit. The device is characterised in that in the illumination optical path for at least two of the illumination sub-beams a controllable beam manipulation means is present for independent setting of a spectral composition of the respective illumination sub-beam, and the control and evaluation unit is designed to control the beam manipulation means. The invention further relates to a method for multispot scanning microscopy.

    MICROSCOPE AND MICROSCOPY TECHNIQUES
    3.
    发明申请
    MICROSCOPE AND MICROSCOPY TECHNIQUES 审中-公开
    显微镜和显微技术

    公开(公告)号:US20170068082A1

    公开(公告)日:2017-03-09

    申请号:US15298424

    申请日:2016-10-20

    摘要: A microscope with at least one illumination beam that is phase modulated in a section along its cross-section with a modulation frequency and a microscope lens for focusing the illumination beam into a test as well as a detection beam path and at least one means of demodulation, wherein at least one polarization altering item is scheduled in the illuminating beam path, for which a phase plate is subordinated that exhibits at least two areas with different phase influence.A second embodiment involves a microscope with at least one illumination beam and a microscope lens for focusing the illumination beam into a test as well as a detection beam path with at least one demodulator, wherein the illumination beam is set up alternatingly on at least two beam paths, and an element in one of the beam paths is scheduled to generate a field mode different from that of the first beam path, and the two beam paths are overlaid with different field modes in the focus.

    摘要翻译: 第二实施例涉及具有至少一个照明光束的显微镜和用于将照明光束聚焦成具有至少一个解调器的测试以及检测光束路径的显微镜透镜,其中照明光束在至少两个光束上交替地设置 路径和其中一个光束路径中的元素被调度以产生与第一光束路径不同的场模式,并且两个光束路径在焦点中被覆盖有不同的场模式。

    LASER SCANNING MICROSCOPE APPARATUS AND LASER SCANNING METHOD
    4.
    发明申请
    LASER SCANNING MICROSCOPE APPARATUS AND LASER SCANNING METHOD 审中-公开
    激光扫描显微镜装置和激光扫描方法

    公开(公告)号:US20150116822A1

    公开(公告)日:2015-04-30

    申请号:US14527504

    申请日:2014-10-29

    发明人: Keigo MORI

    摘要: Both light stimulation of a specimen at a high intensity and detailed fluorescence observation at a low intensity are realized by using a single laser light source. Provided is a laser scanning microscope apparatus (1) including a laser light source (11) that generates laser light; output-power control portion (13) that can set an output power of the laser light source (11) by changing the output power in a step-wise manner; an AOTF (15) that can adjust a light level of the laser light emitted from the laser light source (11) in a step-wise manner at a resolution that is finer than a resolution at which the output-power control portion (13) changes the output power of the laser light source (11); and an observation device (17) that scans the laser light whose light level has been adjusted by the AOTF (15) on a specimen and that detects fluorescence generated at the specimen.

    摘要翻译: 通过使用单个激光源实现高强度的样品的光刺激和低强度的详细荧光观察。 本发明提供一种包括产生激光的激光光源(11)的激光扫描显微镜装置(1) 输出功率控制部(13),其能够通过逐步地改变输出功率来设定激光光源(11)的输出功率; AOTF(15),其可以以比分辨率更精细地调整从激光光源(11)发射的激光的光级别以逐步的方式调节亮度,该分辨率比输出功率控制部分(13)的分辨率更精细, 改变激光光源(11)的输出功率; 以及观察装置(17),其对通过试样上的AOTF(15)调整了光水平的激光进行扫描,并且检测在样本上产生的荧光。

    METHOD FOR OPTICAL FREQUENCY SYNTHESIS
    6.
    发明申请
    METHOD FOR OPTICAL FREQUENCY SYNTHESIS 有权
    光学频率合成方法

    公开(公告)号:US20140355631A1

    公开(公告)日:2014-12-04

    申请号:US14294217

    申请日:2014-06-03

    发明人: Yuriy MAYZLIN

    IPC分类号: H01S3/139

    摘要: A method for generation of electromagnetic radiation has the following method steps: generation of electromagnetic radiation at a useful frequency, division of the electromagnetic radiation into a useful beam and a secondary beam, frequency shift of the electromagnetic radiation of the secondary beam, control of the useful frequency as determined by a manipulated variable, wherein the manipulated variable is derived from the frequency-shifted radiation of the secondary beam.

    摘要翻译: 产生电磁辐射的方法具有以下方法步骤:在有用频率下产生电磁辐射,将电磁辐射分成有用光束和次光束,次级光束的电磁辐射的频移, 由操纵变量确定的有用频率,其中所述操纵变量从所述次级光束的频移辐射导出。

    METHOD AND DEVICE FOR ACOUSTO-OPTIC FILTERING WITH LONG OPTICAL AND ACOUSTIC INTERACTION LENGTH
    7.
    发明申请
    METHOD AND DEVICE FOR ACOUSTO-OPTIC FILTERING WITH LONG OPTICAL AND ACOUSTIC INTERACTION LENGTH 审中-公开
    用于具有长光和交流相互作用长度的光学滤波的方法和装置

    公开(公告)号:US20120069427A1

    公开(公告)日:2012-03-22

    申请号:US12888033

    申请日:2010-09-22

    IPC分类号: G02F1/33

    CPC分类号: G02F1/116 G02F2201/17

    摘要: A method and a device for acousto-optic filtering with large optic and acoustic interaction length; includes the use of a birefringent acousto-optic crystal whereof the sound wave propagation speed is as low as possible, this acousto-optical crystal including, on one of its faces, a piezoelectric transducer intended to generate a transverse sound wave whereof the energy propagates collinearly to the energy of an incident light wave, all along the path of the incident light wave, in the aforementioned birefringent acousto-optic crystal, knowing that the transverse sound wave and the incident light wave travel a path including multiple reflections on one or the other of the reflective faces of the birefringent acousto-optic crystal perpendicular to the axes of symmetry shared by the acoustic slownesses curve and the curves of the ordinary and extraordinary optical indices of the acousto-optic crystal.

    摘要翻译: 一种具有大光学和声学相互作用长度的声光滤波的方法和装置; 包括使用双折射声光晶体,其声波传播速度尽可能低,该声光晶体在其一个面上包括用于产生横向声波的压电换能器,其中能量共线地传播 在上述双折射声光晶体中,所有沿着入射光波的路径的入射光波的能量,知道横向声波和入射光波在一个或另一个上行进包括多个反射的路径 的双折射声光晶体的反射面垂直于由声音缓慢曲线共享的对称轴以及声光晶体的普通和非凡光学指数的曲线。

    Method and device for high resolution acousto-optic programmable filtering in the infrared region
    8.
    发明授权
    Method and device for high resolution acousto-optic programmable filtering in the infrared region 有权
    在红外区域进行高分辨率声光可编程滤波的方法和装置

    公开(公告)号:US07944607B2

    公开(公告)日:2011-05-17

    申请号:US12237354

    申请日:2008-09-24

    申请人: Pierre Tournois

    发明人: Pierre Tournois

    IPC分类号: G02F1/33

    CPC分类号: G02F1/116

    摘要: The subject matter of the invention is a method of high-resolution acousto-optic programmable filtering in the infrared region of an incident optical wave. To that end it proposes the use of a birefringent acousto-optic crystal whereof the propagation speed of acoustic waves is slow, such as compounds of mercury, which acousto-optic crystal comprises, on one of its faces, a piezoelectric transducer designed to generate a transverse acoustic wave with wave vector whereof the energy propagates according to the same axis but in the opposite direction to the energy of the incident optical wave, knowing that the optical wave resulting from the acousto-optic interaction between the incident optical wave and the acoustic wave with wave vector is diffracted perpendicularly or almost perpendicularly to the direction of the incident optical wave.

    摘要翻译: 本发明的主题是在入射光波的红外区域中的高分辨率声光可编程滤波的方法。 为此,它提出使用双折射声光晶体,其声波的传播速度较慢,例如汞的化合物,其声光晶体在其一个面上包括设计成产生 具有波矢的横向声波,其能量根据相同的轴传播,但与入射光波的能量相反的方向传播,知道由入射光波和声波之间的声光相互作用产生的光波 其中波矢量垂直于或几乎垂直于入射光波的方向衍射。

    Acousto-optic devices
    9.
    发明授权
    Acousto-optic devices 失效
    声光设备

    公开(公告)号:US07894125B2

    公开(公告)日:2011-02-22

    申请号:US11795185

    申请日:2007-05-10

    IPC分类号: G02F1/33

    摘要: An acousto-optic module is provided, including a number of partially coupled optical resonators distributed within a dielectric medium and at least one acoustic transducer mounted on a surface of the dielectric medium for injecting an acoustic wave into the optical resonators so as to diffract light passing therethrough by means of Bragg diffraction. This acousto-optic module has been applied in particular to an improved tuneable optical filter in which an acoustic shear wave is generated and which travels through the acousto-optic module in a direction substantially parallel with a polarized light signal passing therethrough. The acousto-optic module is also applied to an improved optical frequency shifter.

    摘要翻译: 提供了一种声光模块,其包括分布在电介质介质中的多个部分耦合的光谐振器,以及安装在介质介质表面上的至少一个声换能器,用于将声波注入到光谐振器中,以便将光通过 通过布拉格衍射。 该声光模块已经特别应用于改进的可调谐光学滤波器,其中产生声剪切波,并且在与通过其的偏振光信号基本平行的方向上传播声光模块。 声光模块也适用于改进的光学移频器。