摘要:
The invention relates to a device for multispot scanning microscopy, having a multicolour light source for providing at least one illumination light beam, having a splitting device for splitting the illumination light beam into a plurality of illumination sub-beams, having first optical means for providing an illumination optical path for guiding and focussing the individual illumination sub-beams respectively into a light spot on or in a specimen to be examined, having a scan unit for guiding the light spots over the probe, having a detection unit for detecting detection light emitted by the specimen in detection sub-beams after irradiation with the individual illumination sub-beams, having second optical means for providing a detection optical path for guiding the detection sub-beams to the detector unit, having a control and evaluation unit for controlling the scan unit and for evaluating the detection light detected by the detection unit. The device is characterised in that in the illumination optical path for at least two of the illumination sub-beams a controllable beam manipulation means is present for independent setting of a spectral composition of the respective illumination sub-beam, and the control and evaluation unit is designed to control the beam manipulation means. The invention further relates to a method for multispot scanning microscopy.
摘要:
Provided are a light source apparatus and an inspection apparatus that can stably output a wavelength converted light beam. A light source apparatus includes a laser light source that generates a first fundamental light beam, at least one nonlinear optical crystal that generates a wavelength converted light beam using the fundamental light beam or a harmonic laser beam of the fundamental light beam as an incident light beam, a detector that detects the wavelength converted light beam, an acousto-optic modulator that is disposed in an optical path of the incident light beam in such a way that a zero-order light beam enters the nonlinear optical crystal, and a controller that controls an output intensity of the wavelength converted light beam according to a detection signal from the detector.
摘要:
A microscope with at least one illumination beam that is phase modulated in a section along its cross-section with a modulation frequency and a microscope lens for focusing the illumination beam into a test as well as a detection beam path and at least one means of demodulation, wherein at least one polarization altering item is scheduled in the illuminating beam path, for which a phase plate is subordinated that exhibits at least two areas with different phase influence.A second embodiment involves a microscope with at least one illumination beam and a microscope lens for focusing the illumination beam into a test as well as a detection beam path with at least one demodulator, wherein the illumination beam is set up alternatingly on at least two beam paths, and an element in one of the beam paths is scheduled to generate a field mode different from that of the first beam path, and the two beam paths are overlaid with different field modes in the focus.
摘要:
Both light stimulation of a specimen at a high intensity and detailed fluorescence observation at a low intensity are realized by using a single laser light source. Provided is a laser scanning microscope apparatus (1) including a laser light source (11) that generates laser light; output-power control portion (13) that can set an output power of the laser light source (11) by changing the output power in a step-wise manner; an AOTF (15) that can adjust a light level of the laser light emitted from the laser light source (11) in a step-wise manner at a resolution that is finer than a resolution at which the output-power control portion (13) changes the output power of the laser light source (11); and an observation device (17) that scans the laser light whose light level has been adjusted by the AOTF (15) on a specimen and that detects fluorescence generated at the specimen.
摘要:
A method and a system for scanning a time delay between a first ultrafast optical pulse of duration shorter than 10 ps and a second ultrafast optical pulse of duration shorter than 10 ps, wherein the second ultrafast pulse is submitted to an acousto-optic Bragg diffraction by an acoustic pulse in the bulk of an acousto-optic material and the delay scanning is produced by time variation of the acoustic pulse in the material.
摘要:
A method for generation of electromagnetic radiation has the following method steps: generation of electromagnetic radiation at a useful frequency, division of the electromagnetic radiation into a useful beam and a secondary beam, frequency shift of the electromagnetic radiation of the secondary beam, control of the useful frequency as determined by a manipulated variable, wherein the manipulated variable is derived from the frequency-shifted radiation of the secondary beam.
摘要:
A method and a device for acousto-optic filtering with large optic and acoustic interaction length; includes the use of a birefringent acousto-optic crystal whereof the sound wave propagation speed is as low as possible, this acousto-optical crystal including, on one of its faces, a piezoelectric transducer intended to generate a transverse sound wave whereof the energy propagates collinearly to the energy of an incident light wave, all along the path of the incident light wave, in the aforementioned birefringent acousto-optic crystal, knowing that the transverse sound wave and the incident light wave travel a path including multiple reflections on one or the other of the reflective faces of the birefringent acousto-optic crystal perpendicular to the axes of symmetry shared by the acoustic slownesses curve and the curves of the ordinary and extraordinary optical indices of the acousto-optic crystal.
摘要:
The subject matter of the invention is a method of high-resolution acousto-optic programmable filtering in the infrared region of an incident optical wave. To that end it proposes the use of a birefringent acousto-optic crystal whereof the propagation speed of acoustic waves is slow, such as compounds of mercury, which acousto-optic crystal comprises, on one of its faces, a piezoelectric transducer designed to generate a transverse acoustic wave with wave vector whereof the energy propagates according to the same axis but in the opposite direction to the energy of the incident optical wave, knowing that the optical wave resulting from the acousto-optic interaction between the incident optical wave and the acoustic wave with wave vector is diffracted perpendicularly or almost perpendicularly to the direction of the incident optical wave.
摘要:
An acousto-optic module is provided, including a number of partially coupled optical resonators distributed within a dielectric medium and at least one acoustic transducer mounted on a surface of the dielectric medium for injecting an acoustic wave into the optical resonators so as to diffract light passing therethrough by means of Bragg diffraction. This acousto-optic module has been applied in particular to an improved tuneable optical filter in which an acoustic shear wave is generated and which travels through the acousto-optic module in a direction substantially parallel with a polarized light signal passing therethrough. The acousto-optic module is also applied to an improved optical frequency shifter.
摘要:
An apparatus includes a first optical amplifier that uses a rare-earth-doped optical medium, an isolator that inputs amplified light amplified by the first optical amplifier, a second optical amplifier that uses a rare-earth-doped optical medium to amplify a light output from the isolator, and a first light router that routes amplified spontaneous emission light generated by the first optical amplifier or the second optical amplifier to input, by a second light router, the routed amplified spontaneous emission light to the optical rare-earth-doped medium other than the optical rare-earth-doped medium where the routed amplified spontaneous emission light is generated.