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    METHODS FOR IDENTIFYING COMPOUNDS THAT BIND TO A TARGET
    2.
    发明申请
    METHODS FOR IDENTIFYING COMPOUNDS THAT BIND TO A TARGET 无效
    将化合物鉴定到目的物的方法

    公开(公告)号：US20030100007A1

    公开(公告)日：2003-05-29

    申请号：US08769250

    申请日：1996-12-18

    发明人： HOWARD BENJAMIN ,  MARK A. FINDEIS ,  MALCOLM L. GEFTER ,  GARY MUSSO ,  ETHAN R. SIGNER

    IPC分类号： G01N033/53 ,  G01N033/567 ,  G01N033/566 ,  G01N033/543 ,  G01N033/554

    CPC分类号： C07K1/047

    摘要： Methods for identifying a compound that binds to a target are described. In general, the methods involve forming a first library comprising a multiplicity of peptides, identifying one or more peptides that bind to the target and determining a peptide motif therefrom, forming a second library comprising a multiplicity of compounds designed based on the peptide motif, selecting from the second library at least one compound that binds to the target, and determining the structure or structures of the at least one compound that binds to the target. Libraries of compounds based on a peptide motif and compounds identified by the methods of the invention are also disclosed.

    摘要翻译： 描述了用于鉴定与靶标结合的化合物的方法。 通常，所述方法包括形成包含多个肽的第一文库，鉴定结合靶标的一种或多种肽，并从其中确定肽基序，形成包含基于肽基序设计的多种化合物的第二文库，选择 来自第二文库的至少一种与靶标结合的化合物，以及确定结合靶标的至少一种化合物的结构或结构。 还公开了基于肽基序的化合物和通过本发明的方法鉴定的化合物的文库。

    MULTIHUED LABELS
    5.
    发明申请
    MULTIHUED LABELS 失效
    多媒体标签

    公开(公告)号：US20010044116A1

    公开(公告)日：2001-11-22

    申请号：US09332613

    申请日：1999-06-14

    发明人： LAWRENCE M. KAUVAR ,  JOHN SEDAT

    IPC分类号： G01N033/53 ,  G01N033/542 ,  G01N033/537 ,  G01N033/543 ,  C07K016/00 ,  G01N033/566 ,  G01N033/531

    CPC分类号： G01N33/54313 ,  G01N33/585 ,  G01N33/68 ,  Y10S977/918 ,  Y10S977/924 ,  Y10S977/925

    摘要： Particulate labels that can be individually identified comprise particulate supports to which are bound at least two distinguishable signal-generating moieties, such as fluorophores emitting at different wavelengths, which signals are detectable and measurable in situ. By varying the ratio and/or amounts of the signal-generating moieties, a multiplicity of different and distinguishable labels is obtained. Each different label can then be coupled to a different reagent and the individual interactions of each reagent with a target observed in parallel.

    摘要翻译： 可以单独鉴定的颗粒状标签包括结合至少两个可区分的信号产生部分的颗粒载体，例如以不同波长发射的荧光团，该信号可原位检测和可测量。 通过改变信号产生部分的比例和/或量来获得多个不同且可区别的标签。 然后可以将每个不同的标记偶联到不同的试剂上，并且每个试剂与平行观察到的目标的各个相互作用。

    METHODS FOR THE CHARACTERIZATION AND SELECTION OF RNA TARGET MOTIFS THAT BIND COMPOUNDS OF PHARMACEUTICAL USE
    6.
    发明申请
    METHODS FOR THE CHARACTERIZATION AND SELECTION OF RNA TARGET MOTIFS THAT BIND COMPOUNDS OF PHARMACEUTICAL USE 有权
    用于表征和选择药物使用的化合物的RNA靶标运动的方法

    公开(公告)号：US20010007745A1

    公开(公告)日：2001-07-12

    申请号：US09297479

    申请日：1999-04-30

    发明人： CATHERINE DENISE PRESCOTT

    IPC分类号： C12Q001/68 ,  G01N033/566 ,  C07H021/04

    CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/13

    摘要： Methods are disclosed for identifying an RNA fragment that mimics the structure of a defined or undefined target RNA molecule to which a compound binds inside of a cell resulting in retardation of cell growth or cell death. Methods using these RNA fragments for identifying unknown compounds of pharmaceutical interest, and for identifying unknown RNA targets for use in treating disease are disclosed. These methods and compositions are used in screening for novel antibiotics, bacteriostatics, or modifications thereof or for identifying compounds useful to alter expression levels of proteins encoded by mRna. The methods involve providing random DNA fragments from DNA which encodes RNA target molecules, cloning such fragments to create a plasmid library of same; transfecting cells which contain the native RNA target molecule with the plasmid library and exposing the cells to one or more of test compounds. Cells transfected with an RNA fragment that mimics the target molecule will survive in culture, and cells transfected with fragments which do not mimic the molecule will suffer defects in growth. The mimicking RNA fragment is isolated and provides structural information about the target. The fragment and the information derived from it are then used to screen or design binding compounds.

    摘要翻译： 公开了用于鉴定模拟定义的或未定义的靶RNA分子的结构的RNA片段的方法，化合物结合到细胞内，导致细胞生长或细胞死亡的延迟。 公开了使用这些RNA片段鉴定药物感兴趣的未知化合物以及鉴定用于治疗疾病的未知RNA靶标的方法。 这些方法和组合物用于筛选新型抗生素，抑菌剂或其修饰物，或用于鉴定可用于改变由mRna编码的蛋白质的表达水平的化合物。 该方法涉及从编码RNA靶分子的DNA提供随机DNA片段，克隆这些片段以产生相同的质粒文库; 将含有天然RNA靶分子的细胞转染到质粒文库中，并将细胞暴露于一种或多种测试化合物。 用模拟靶分子的RNA片段转染的细胞将在培养物中存活，并且用不模拟分子的片段转染的细胞将在生长中遭受缺陷。 分离模拟RNA片段并提供关于靶标的结构信息。 然后将片段和从其得到的信息用于筛选或设计结合化合物。

    Methods for diagnosing thyroid conditions and for monitoring thyroxine therapy
    8.
    发明申请
    Methods for diagnosing thyroid conditions and for monitoring thyroxine therapy 审中-公开
    诊断甲状腺疾病和监测甲状腺素治疗的方法

    公开(公告)号：US20020076827A1

    公开(公告)日：2002-06-20

    申请号：US09915931

    申请日：2001-07-26

    发明人： Hilton A. Salhanick ,  Joachim Hourihan

    IPC分类号： G01N033/566 ,  G01N033/543

    CPC分类号： G01N33/76 ,  G01N33/78

    摘要： This invention provides a method of diagnosing a thyroid condition in a subject which comprises: determining the concentration of thyroid stimulating hormone in a urine sample by a method which is not a radioimmunoassay; and comparing the concentration of thyroid stimulating hormone with a urinary concentration of thyroid stimulating hormone in a normal subject; wherein: i) a concentration of thyroid stimulating hormone which is higher than the urinary concentration of thyroid stimulating hormone in the normal subject diagnoses hypothyroidism in the subject; and ii) a concentration of thyroid stimulating hormone which is lower than the urinary concentration of thyroid stimulating hormone in the normal subject diagnoses hyperthyroidism in the subject. This invention also proves a method of monitoring thyroxine therapy.

    摘要翻译： 本发明提供一种诊断受试者甲状腺疾病的方法，其包括：通过不是放射免疫测定法测定尿样中的促甲状腺激素的浓度; 并比较正常受试者的促甲状腺激素浓度与促甲状腺激素的尿浓度; 其中：i）在正常受试者诊断甲状腺功能减退症中甲状腺激素激素的浓度高于促甲状腺激素的尿浓度; 和ii）在正常受试者诊断甲状腺功能亢进症中甲状腺激素激素的浓度低于促甲状腺激素激素的浓度。 本发明还证明了一种监测甲状腺素治疗的方法。