-
公开(公告)号：US20240294866A1
公开(公告)日：2024-09-05
申请号：US18270490
申请日：2021-12-29
发明人： Gang MENG , Huiping JIA , Aiying WEI , Chunguang ZHAO , Houbo SU , Lipeng YANG , Fengyong MA , Xiaoqun ZHOU , Xiaowei GUO , Bin TIAN
CPC分类号： C12N1/205 , C07K14/34 , C12N15/77 , C12P13/14 , C12R2001/15
摘要： Provided is a method for introducing a point mutation to a BBD29_04920 gene coding sequence in Corynebacterium or improving the expression thereof. The point mutation causes a mutation to the base at position 1560 in the BBD29_04920 gene sequence from cytosine (C) to adenine (A) such that asparagine at position 520 of a coded corresponding amino acid sequence is substituted by lysine. The method can increase fermentation yield of glutamic acid in a strain with the mutation. Also provided are the bacterium generating L-glutamic acid, a nucleic acid and protein comprising the mutation, a recombinant vector and recombinant strain comprising the nucleic acid, and use of these biomaterials in the regulation of the production of L-glutamic acid of a microorganism.
-
公开(公告)号：US20230242923A1
公开(公告)日：2023-08-03
申请号：US18063629
申请日：2022-12-08
CPC分类号： C12N15/62 , C12N15/8243 , C12N15/8251 , C12P13/02 , C12P13/04 , C12N15/8257 , C12P13/14 , Y10T436/174614
摘要： Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods at the present invention comprises cocoyl glutamate.
-
公开(公告)号：US20220348897A1
公开(公告)日：2022-11-03
申请号：US17573877
申请日：2022-01-12
申请人： ENOBRAQ
发明人： Cedric BOISART , Nicolas MORIN
摘要： The invention concerns a genetically modified microorganism expressing a functional type I or II RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which the glycolysis pathway is at least partially inhibited, said microorganism being genetically modified so as to produce an exogenous molecule and/or to overproduce an endogenous molecule. According to the invention, the oxidative branch of the pentose phosphate pathway may also be at least partially inhibited. The invention also concerns the use of such a genetically modified microorganism for the production or overproduction of a molecule of interest and processes for the synthesis or bioconversion of molecules of interest.
-
4.发明申请公开(公告)号：US20220282265A1
公开(公告)日：2022-09-08
申请号：US17632497
申请日：2020-08-10
申请人： Zymergen Inc.
摘要： The present disclosure describes the engineering of microbial cells for fermentative production of 3-amino-4-hydroxybenzoic acid and provides novel engineered microbial cells and cultures, as well as related 3-amino-4-hydroxybenzoic acid production methods. Embodiments 1: An engineered microbial cell that produces 3-amino-4-hydroxybenzoic acid, wherein the engineered microbial cell expresses: (a) a non-native 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy) heptanoate synthase; and (b) a non-native 3-amino-4-benzoic acid synthase.
-
公开(公告)号：US20220033763A1
公开(公告)日：2022-02-03
申请号：US17309448
申请日：2019-09-20
发明人： Tomokazu Ino
摘要： A method for producing a rich taste imparting substance-containing yeast, where the method includes: a yeast proliferating step of culturing a yeast that is modified to have a reduced acetolactate synthase activity in cells, and has isoleucine and valine requirements in a culture medium containing isoleucine and valine, to proliferate the yeast; and a rich taste imparting substance producing step of culturing the yeast with addition of valine to the culture medium when an isoleucine content in the culture medium is less than 0.2% by mass, to produce a rich taste imparting substance, wherein the rich taste imparting substance is at least one of γ-Glu-Abu and γ-Glu-Abu-Gly.
-
公开(公告)号：US20210403957A1
公开(公告)日：2021-12-30
申请号：US17277211
申请日：2019-09-17
发明人： Jie LIN , Hongxian XU , Hui PENG
摘要： Provided is an application of trehalase in fermentative production. The trehalase has amino acid sequences shown in SEQ ID NO.6, SEQ ID NO.7, and SEQ ID NO.8. Provided are methods for producing and applying trehalase, particularly being applied in the production and fermentation of alcohol and an amino acid.
-
公开(公告)号：US20200277592A1
公开(公告)日：2020-09-03
申请号：US16480569
申请日：2018-01-26
申请人： ENOBRAQ
发明人： Cedric BOISART , Nicolas MORIN
摘要： The invention concerns a genetically modified microorganism expressing a functional type I or II RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which the glycolysis pathway is at least partially inhibited, said microorganism being genetically modified so as to produce an exogenous molecule and/or to overproduce an endogenous molecule. According to the invention, the oxidative branch of the pentose phosphate pathway may also be at least partially inhibited. The invention also concerns the use of such a genetically modified microorganism for the production or overproduction of a molecule of interest and processes for the synthesis or bioconversion of molecules of interest.
-
公开(公告)号：US20180355388A1
公开(公告)日：2018-12-13
申请号：US16106830
申请日：2018-08-21
申请人： AJINOMOTO CO., INC.
发明人： Anastasia Yurievna Romkina , Alexey Nikolaevich Chernyshov , Yulia Georgievna Rostova , Elvira Borisovna Voroshilova , Mikhail Yurievich Kiryukhin
IPC分类号： C12P13/04 , C07K14/245 , C12P13/08 , C12P13/10 , C12P13/12 , C12P13/14 , C12P13/24 , C12P13/06
CPC分类号： C12P13/04 , C07K14/245 , C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12P13/14 , C12P13/24
摘要： The present invention provides a method for producing L-amino acids by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding an iron exporter, such as a fetB gene, fetA gene, fieF gene, or a combination of these genes.
-
9.发明申请公开(公告)号：US20180312886A1
公开(公告)日：2018-11-01
申请号：US16030262
申请日：2018-07-09
申请人： CARGILL INCORPORATED
IPC分类号： C12P7/64 , C12N15/70 , C07C53/126 , C12P13/14 , C12N9/02 , C12P7/52 , C12P7/42 , C12P7/22 , C12P7/00 , C12N9/10 , C12P7/62
摘要： Metabolically engineered microorganism strains are disclosed, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product. Such chemical products include polyketides, 3-hydroxypropionic acid, and various other chemical products described herein. Methods of production also may be applied to further downstream products, such as consumer products. In various embodiments, modifications to a microorganism and/or culture system divert, at least transiently, usage of malonyl-coA from the fatty acid biosynthesis pathway and thereby provides for usage of the malonyl-coA for a chemical product other than a fatty acid. In various embodiments, the fatty acid biosynthesis pathway is modulated to produce specific fatty acids or combinations of fatty acids.
-
公开(公告)号：US20180312882A1
公开(公告)日：2018-11-01
申请号：US16034532
申请日：2018-07-13
申请人： Kao Corporation
发明人： Kougo Iida , Takumi Iwasaki , Tatsunari Nishi
IPC分类号： C12P7/42 , C12N15/52 , C12P7/44 , C12P7/40 , C12N1/20 , C12N9/10 , C12P13/14 , C12P13/06 , C12P13/20
CPC分类号： C12P7/42 , C12N1/20 , C12N9/1055 , C12N15/52 , C12P7/40 , C12P7/44 , C12P13/06 , C12P13/14 , C12P13/20
摘要： It is an object of the present invention to provide a bacterial strain that can decrease the amount of an intermediate Compound P converted into Metabolite M and efficiently accumulate Compound P in a medium that is not supplemented with Metabolite M or the final product generated from Metabolite M. The present invention provides a prokaryotic organism having all features (a) to (d) as defined in the specification so as to accumulate Compound P by regulating expression level of Enzyme X that converts Compound P as an intermediate metabolite into Metabolite M in a biosynthetic pathway in which Metabolite M indispensable for the growth is produced from a carbon source.
-
-
-
-
-
-
-
-
-
搜索结果
536 条记录 (耗时 0.045 秒)
