公开(公告)号：US11566270B2

公开(公告)日：2023-01-31

申请号：US17219792

申请日：2021-03-31

申请人： Braskem S.A.

发明人： Paulo Moises Raduan Alexandrino ,  Iuri Estrada Gouvea ,  Veronica Leite Queiroz ,  Felipe Cicaroni Fernandes ,  Bárbara Mano

IPC分类号： C12P17/04 ,  C07D307/68 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/08 ,  C12N9/16

摘要： The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US11535601B2

公开(公告)日：2022-12-27

申请号：US16975894

申请日：2018-07-30

申请人： Jiangsu Ruike Medical Science And Technology Co., Ltd.

发明人： Zhaobo Gao ,  Jianhua Chen ,  Zhidong Wan ,  Dawei He ,  Zengle Zhou ,  Xiaodong Ma ,  Wei Xiang ,  Jingxin Lin ,  Yijiang Mei

IPC分类号： C07D307/33 ,  C07D493/04 ,  C12P17/04 ,  C12P17/16

摘要： The invention relates to the field of pharmaceutical synthesis, in particular to a preparation method of (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol derivatives and their intermediates. The preparation method is carried out starting from compound Formula A1. In the preparation of (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol derivatives, the chirality was constructed by enzymatic method, and the products were prepared with high optical purity. The preparation method can be used to produce the key intermediates of (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol of darunavir commercially, which is a very economical route suitable for industrial production.

公开(公告)号：US11525123B2

公开(公告)日：2022-12-13

申请号：US17200394

申请日：2021-03-12

申请人： California Institute of Technology

发明人： Kai Chen ,  Noah P. Dunham ,  Daniel J. Wackelin ,  Andrew Z. Zhou ,  Zhen Liu

IPC分类号： C12N9/02 ,  C12P17/04 ,  C12P7/62 ,  C12P17/16

摘要： The present disclosure provides cytochrome P450 variants useful for carrying out in vivo and in vitro carbene insertion reactions. Methods for preparing carbene insertion products including cyclopropenes, cyclopropanes, bicyclobutanes, substituted lactones, cyclized compounds, and substituted amines are also described.

公开(公告)号：US11421212B2

公开(公告)日：2022-08-23

申请号：US16323458

申请日：2017-08-04

申请人： CARGILL, INCORPORATED

发明人： Christopher K. Miller ,  Gregory Michael Poynter ,  Amit Vas

IPC分类号： C12N9/34 ,  C12P7/06 ,  C12P13/04 ,  C12P17/04 ,  C12P19/14

摘要： The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).

公开(公告)号：US20220204953A1

公开(公告)日：2022-06-30

申请号：US17694779

申请日：2022-03-15

申请人： Jiangnan University

发明人： Liming Liu ,  Xin Xu ,  Wei Song ,  Xiulai Chen ,  Jia Liu ,  Cong Gao ,  Jing Wu ,  Liang Guo

IPC分类号： C12N9/16 ,  C12N15/70 ,  C12P17/04

摘要： Disclosed is a mutant of acid phosphatase and an application thereof, belonging to the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, a catalytic system of the mutant of acid phosphatase PaAPaseMu3 is optimized from the aspects of reaction pH and temperature, so that a yield of L-ascorbate-2-phosphate can reach 90.1 g/L and a molar yield can reach 75.1%. Therefore, the problems of a high substrate cost, environmental pollution and the like before are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

公开(公告)号：US11371046B2

公开(公告)日：2022-06-28

申请号：US15270578

申请日：2016-09-20

申请人： Genomatica, Inc.

发明人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

IPC分类号： C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12P7/52 ,  C12P17/04 ,  B01D3/00 ,  C12N15/70 ,  C12N15/81

摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

公开(公告)号：US20220064682A1

公开(公告)日：2022-03-03

申请号：US17463988

申请日：2021-09-01

申请人： Braskem S.A.

发明人： Iuri Estrada GOUVEA ,  Ana Karina BRAMBILLA COSTA ,  Marcos Rogerio SIMOES ,  Veronica Leite QUEIROZ ,  Aline Silva ROMÃO DUMARESQ

IPC分类号： C12P17/04 ,  C12N1/16 ,  C12N15/81

摘要： The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandimethanol from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandimethanol with ethanol and for co-producing 2,4-furandimethanol with ethanol and acetone and/or isopropanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandimethanol and that couple the 2,4-furandimethanol pathway with an additional metabolic pathway. The present disclosure further provides enzymatic production of 2,4-furandicarboxylic acid.

公开(公告)号：US11203774B2

公开(公告)日：2021-12-21

申请号：US16842401

申请日：2020-04-07

申请人： The Regents of the University of California

发明人： John Eugene Dueber ,  Zachary Nicholas Russ ,  Tammy Melody Hsu ,  Terry Don Johnson, Jr. ,  Bernardo Cervantes ,  Ramya Lakshmi Prathuri ,  Shyam Pravin Bhakta ,  Arthur Muir Fong, III ,  Luke Nathaniel Latimer

IPC分类号： C12P17/10 ,  C12P17/04 ,  C12N9/02 ,  C12N9/24 ,  C12P19/60 ,  C12N9/10 ,  C12P17/16 ,  C09B7/02 ,  C09B7/04 ,  C12N9/14

摘要： The present disclosure relates to the biosynthesis of indigoid dye precursors and their conversion to indigoid dyes. Specifically, the present disclosure relates to methods of using polypeptides to produce indigoid dye precursors from indole feed compounds, and the use of the indigoid dye precursors to produce indigoid dyes.

公开(公告)号：US20210388403A1

公开(公告)日：2021-12-16

申请号：US17291821

申请日：2019-11-08

申请人： INSTITUT DES SCIENCES ET INDUSTRIES DU VIVANT ET DE L'ENVIRONNEMENT - AGROPARIS TECH ,  University of Florida Research Foundation, Inc.

发明人： Florent Allais ,  Louis Mouterde ,  John Dale Stewart

IPC分类号： C12P17/04

摘要： An eco-compatible method is used to synthesize 2H-HBO optionally substituted at the β-position of the lactone function from LGO or a saturated form of LGO such as dihydrolevoglucosenone (2H-LGO) or LGO hydrate (OH-LGO) via a biocatalytic reaction using a cyclohexanone monooxygenase (CHMO).

公开(公告)号：US20210381013A1

公开(公告)日：2021-12-09

申请号：US17131930

申请日：2020-12-23

申请人： BASF SE

发明人： Michael BREUER ,  Wolfgang SIEGEL ,  Stefan RÜDENAUER ,  Ralf PELZER

IPC分类号： C12P17/04

摘要： The present invention relates to processes for the preparation of sclareolide and related compounds by the biocatalytic cyclization of polyunsaturated carboxylic acid compounds, in particular of homofarnesylic acid and related compounds; and to a process for the preparation of ambroxide via the biocatalytic cyclization of homofarnesylic acid to sclareolide.