公开(公告)号：US12071645B2

公开(公告)日：2024-08-27

申请号：US17900590

申请日：2022-08-31

申请人： Givaudan SA

发明人： Eric Eichhorn ,  Boris Schilling ,  Denis Wahler ,  Laurent Fourage ,  Esther Locher

IPC分类号： C12P17/04 ,  C07D307/92 ,  C12N9/90

CPC分类号： C12P17/04 ,  C07D307/92 ,  C12N9/90

摘要： There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (−)-Ambrox and SHC/HAC enzymes in methods to prepare (−)-Ambrox.

公开(公告)号：US12037623B2

公开(公告)日：2024-07-16

申请号：US17257792

申请日：2019-07-02

申请人： Merck Sharp & Dohme Corp.

发明人： Mark A. Huffman ,  Anna Fryszkowska ,  Joshua N. Kolev ,  Paul N. Devine ,  Kevin R. Campos ,  Matthew Truppo ,  Christopher C. Nawrat

IPC分类号： C12P19/40 ,  C07C33/042 ,  C07F9/113 ,  C07H13/00 ,  C12P7/18 ,  C12P9/00 ,  C12P17/04

CPC分类号： C12P19/40 ,  C07C33/042 ,  C07F9/113 ,  C07H13/00 ,  C12P7/18 ,  C12P9/00 ,  C12P17/04

摘要： The present invention relates to an enzymatic synthesis of 4′-ethynyl-2′-deoxy nucleosides and analogs thereof, for example EFdA, that eliminates the use of protecting groups on the intermediates, improves the stereoselectivity of glycosylation and reduces the number of process steps needed to make said compounds. It also relates to the novel intermediates employed in the process.

公开(公告)号：US20230250390A1

公开(公告)日：2023-08-10

申请号：US17947421

申请日：2022-09-19

申请人： Prospective Research, Inc.

发明人： Dakota Benjamin Hamill ,  Jake J. Cotter

IPC分类号： C12N1/38 ,  C12N15/80 ,  C12N1/12 ,  C12P17/04 ,  C07D307/33 ,  C12P1/04 ,  C12P17/16 ,  C12P1/06

CPC分类号： C12N1/38 ,  C12N15/80 ,  C12N1/12 ,  C12P17/04 ,  C07D307/33 ,  C12P1/04 ,  C12P17/165 ,  C12P1/06

摘要： Methods and compositions herein provide non-naturally occurring γ-butyrolactones (GBLs) in racemic mixtures that increase efficiency and effectiveness of screening for production of antibiotics, and enhance yields and express silent pathways. Non-naturally occurring GBLs were synthesized and found to stimulate antibiotic production in several different streptomycete strains. Antibiotic production by Streptomyces coelicolor was induced by a racemic mixture of non-cognate stereoisomers of VB-D, seven of which are non-naturally occurring. Further, novel A-factor-type GBL analogs stimulated antibiotic production in S. coelicolor. Synthesis in response to the treatment with the non-cognates GBL was observed for known compounds including undecylprodigiosin, desferrioxamine and streptorubin B, as was synthesis of a compound of unknown structure. A group of 37 additional microbial strains was screened by principal component analysis to determine optimal concentrations of each of a panel of four non-cognate synthetic GBLs for addition to cultures with optimal stimulation of secondary metabolites, and large scale fermentations were analyzed and product enhancement by the GBLs was observed.

公开(公告)号：US20230212624A1

公开(公告)日：2023-07-06

申请号：US18018360

申请日：2021-07-29

申请人： Givaudan SA

发明人： Jean-Pierre BARRAS ,  Sylvie MOREL ,  Eric EICHHORN

IPC分类号： C12P17/04 ,  C12N9/90

CPC分类号： C12P17/04 ,  C12N9/90 ,  C12Y504/99017

摘要： A method for purifying a crude flavor or perfumery or cosmetic ingredient or intermediate, for example crude (−)-Ambrox, comprising a number of washing steps, the products of said method, and uses of said product.

公开(公告)号：US11566270B2

公开(公告)日：2023-01-31

申请号：US17219792

申请日：2021-03-31

申请人： Braskem S.A.

发明人： Paulo Moises Raduan Alexandrino ,  Iuri Estrada Gouvea ,  Veronica Leite Queiroz ,  Felipe Cicaroni Fernandes ,  Bárbara Mano

IPC分类号： C12P17/04 ,  C07D307/68 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/08 ,  C12N9/16

摘要： The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US11535601B2

公开(公告)日：2022-12-27

申请号：US16975894

申请日：2018-07-30

申请人： Jiangsu Ruike Medical Science And Technology Co., Ltd.

发明人： Zhaobo Gao ,  Jianhua Chen ,  Zhidong Wan ,  Dawei He ,  Zengle Zhou ,  Xiaodong Ma ,  Wei Xiang ,  Jingxin Lin ,  Yijiang Mei

IPC分类号： C07D307/33 ,  C07D493/04 ,  C12P17/04 ,  C12P17/16

摘要： The invention relates to the field of pharmaceutical synthesis, in particular to a preparation method of (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol derivatives and their intermediates. The preparation method is carried out starting from compound Formula A1. In the preparation of (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol derivatives, the chirality was constructed by enzymatic method, and the products were prepared with high optical purity. The preparation method can be used to produce the key intermediates of (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol of darunavir commercially, which is a very economical route suitable for industrial production.

公开(公告)号：US11525123B2

公开(公告)日：2022-12-13

申请号：US17200394

申请日：2021-03-12

申请人： California Institute of Technology

发明人： Kai Chen ,  Noah P. Dunham ,  Daniel J. Wackelin ,  Andrew Z. Zhou ,  Zhen Liu

IPC分类号： C12N9/02 ,  C12P17/04 ,  C12P7/62 ,  C12P17/16

摘要： The present disclosure provides cytochrome P450 variants useful for carrying out in vivo and in vitro carbene insertion reactions. Methods for preparing carbene insertion products including cyclopropenes, cyclopropanes, bicyclobutanes, substituted lactones, cyclized compounds, and substituted amines are also described.

公开(公告)号：US11421212B2

公开(公告)日：2022-08-23

申请号：US16323458

申请日：2017-08-04

申请人： CARGILL, INCORPORATED

发明人： Christopher K. Miller ,  Gregory Michael Poynter ,  Amit Vas

IPC分类号： C12N9/34 ,  C12P7/06 ,  C12P13/04 ,  C12P17/04 ,  C12P19/14

摘要： The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).

公开(公告)号：US20220204953A1

公开(公告)日：2022-06-30

申请号：US17694779

申请日：2022-03-15

申请人： Jiangnan University

发明人： Liming Liu ,  Xin Xu ,  Wei Song ,  Xiulai Chen ,  Jia Liu ,  Cong Gao ,  Jing Wu ,  Liang Guo

IPC分类号： C12N9/16 ,  C12N15/70 ,  C12P17/04

摘要： Disclosed is a mutant of acid phosphatase and an application thereof, belonging to the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, a catalytic system of the mutant of acid phosphatase PaAPaseMu3 is optimized from the aspects of reaction pH and temperature, so that a yield of L-ascorbate-2-phosphate can reach 90.1 g/L and a molar yield can reach 75.1%. Therefore, the problems of a high substrate cost, environmental pollution and the like before are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

公开(公告)号：US11371046B2

公开(公告)日：2022-06-28

申请号：US15270578

申请日：2016-09-20

申请人： Genomatica, Inc.

发明人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

IPC分类号： C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12P7/52 ,  C12P17/04 ,  B01D3/00 ,  C12N15/70 ,  C12N15/81

摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.