公开(公告)号：US20230057075A1

公开(公告)日：2023-02-23

申请号：US17981135

申请日：2022-11-04

申请人： Clarapath, Inc.

发明人： Cong Zhang ,  Mark Fasciano ,  Partha P. Mitra

IPC分类号： G01N1/06 ,  G01N1/31 ,  G01N35/00

摘要： An automated tape transfer apparatus including a tape feed mechanism feeding a continuous length of an adhesive tape through the automated tape transfer apparatus and a tape applicator applying the adhesive tape to a cutting face of a sample block, wherein a section of the sample block is adhered to the adhesive tape after cutting of the section from the sample block. A slide station transfers the cut section from the adhesive tape to a slide.

公开(公告)号：US11519832B2

公开(公告)日：2022-12-06

申请号：US17035040

申请日：2020-09-28

申请人： TissueVision, Inc.

发明人： Timothy Ragan ,  Chaim Moshe Dimant

IPC分类号： G01N1/06 ,  G01N1/30 ,  G01N1/31 ,  G01N1/42 ,  G01N21/64 ,  G01N35/00 ,  G02B21/16 ,  G02B21/24 ,  G02B21/26 ,  G02B21/36

摘要： The present invention relates to systems and methods for sequential operation of staining, imaging and sectioning of tissue samples by a processing system. After each layer of the sample is removed by the sectioning system, the system automatically stains the exposed surface of a sample to a depth to enable imaging of the remaining tissue. The system then repeats the sectioning, staining and imaging steps in sequence to image the sample.

公开(公告)号：US20220308077A1

公开(公告)日：2022-09-29

申请号：US17702598

申请日：2022-03-23

申请人： Clarapath, Inc.

发明人： Partha P. Mitra ,  Charles Cantor ,  Baris Yagci ,  Jonathan Gardi ,  Dmitry Khavulya

IPC分类号： G01N35/00 ,  G01N1/06 ,  G01N1/28

摘要： Systems and methods enable fully automated tissue processing using tissue processing components for producing tissue sections from a tissue sample block and to transfer the tissue sections to slides. A control device queries a Laboratory Information Management System (LIMS) with a sample identifier associated with the tissue sample block and receives tissue sample data from the LIMS. The control device generates tissue processing workflow parameters based on the tissue sample data for each tissue processing component to perform an automated tissue processing workflow. The control device automatically controls each tissue processing component according to the tissue processing workflow parameters to process the tissue sample block. The control device generates a block processing update message while the tissue sample block is processed and communicates the block processing update message to the LIMS to enable the LIMS to track the automated tissue processing workflow for the tissue sample block.

公开(公告)号：US11445722B2

公开(公告)日：2022-09-20

申请号：US16199266

申请日：2018-11-26

申请人： SHANGHAI CELLIVER BIOTECHNOLOGY INC.

发明人： Hongping Wu ,  Min Zeng ,  Xu Zhou ,  Xiaofang Jin ,  Qiurui Yang

IPC分类号： A01N1/02 ,  C12N5/09 ,  C12N5/00 ,  G01N1/06

摘要： A kit, including: a first solution, a second solution, and a metal mesh. The first solution includes: dulbecco's modified eagle medium (DMEM), 65-95 V/V %; dimethyl sulfoxide (DMSO), 5.5-20 V/V %; ethylene glycol (EG), 3.5-15 V/V %; bovine serum albumin (BSA), 0.5-4 W/V %; sucrose 1-5 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; hetastarch 0.25-0.6 W/V %; and glucose 15-35 W/V %. The second solution includes: DMEM, 65-95 V/V %; DMSO, 5.5-20 V/V %; EG, 8-20 V/V %; BSA, 0.5-4 W/V %; sucrose, 10-20 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; polyvinyl pyrrolidone (PVP), 0.25-0.6 W/V %; and glucose 15-35 W/V %. The metal mesh has a thickness of 0.15-0.2 mm and includes a plurality of square holes. The side length of the square holes is 2.0-3.0 mm, and the spacing between adjacent holes is 0.5-2.0 mm.

公开(公告)号：US11340165B2

公开(公告)日：2022-05-24

申请号：US16629326

申请日：2018-04-10

申请人： HAMAMATSU PHOTONICS K.K.

发明人： Norikazu Sugiyama ,  Masanori Matsubara ,  Satoshi Yamamoto

IPC分类号： G01N21/64 ,  C12Q1/06 ,  G01N1/30 ,  G01N1/06 ,  G01N21/51 ,  G06T7/00 ,  G01N15/14 ,  G01N21/00 ,  G01N1/31 ,  G02B21/00 ,  G02B21/18 ,  G02B21/26 ,  G02B21/36 ,  G01N21/17 ,  G01N15/10

摘要： A sample observation device includes an imaging unit that images observation light generated due to irradiation with the planar light that is transmitted through a membrane filter and outputs fluorescent light image data, a partial image generation unit that specifies a first area corresponding to a first sample holding space and a second area corresponding to a second sample holding space in the fluorescent light image data, and generates first partial image data corresponding to the first area and second partial image data corresponding to the second area, an observation image generation unit that generates first observation image data and second observation image data on the basis of the partial image data, and an analysis unit that analyzes a sample on the basis of the first observation image data and the second observation image data.

公开(公告)号：US11299726B2

公开(公告)日：2022-04-12

申请号：US16255659

申请日：2019-01-23

申请人： Cepheid

发明人： Edwin Wei-Lung Lai ,  Reuel Van Atta ,  Kenneth E. Ho

IPC分类号： C12Q1/68 ,  C12N15/10 ,  G16B25/00 ,  G16B25/10 ,  C12Q1/6806 ,  C12Q1/6886 ,  G01N1/06

摘要： Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.

公开(公告)号：US11260488B2

公开(公告)日：2022-03-01

申请号：US15995018

申请日：2018-05-31

申请人： KOREA BASIC SCIENCE INSTITUTE

发明人： Hwan Hur ,  Geon-Hee Kim ,  Ji Yong Bae ,  Hee-Seok Kweon

IPC分类号： B24B3/36 ,  G01N1/06 ,  B23K26/364

摘要： Provided is a method of manufacturing a diamond knife for an ultra-microtome, including determining a shape and size of a knife, performing a multiphase flow analysis on movement of a droplet at a fore-end of the knife with the determined shape and size, processing a fine pattern to ensure hydrophobic property according to a result of the flow analysis, and grinding a blade of the knife after the processing of the fine pattern to minimize an interval between the pattern and the blade and, thus, a knife blade is always maintained in a wet state due to a droplet movement between a hydrophobic fine pattern and a hydrophilic surface to continuously generate ultrathin slices.

公开(公告)号：US20210222229A1

公开(公告)日：2021-07-22

申请号：US17010390

申请日：2020-09-02

申请人： Quantumcyte, Inc.

发明人： John BUTLER ,  Bidhan CHAUDHURI

IPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  G01N1/06

摘要： Provided herein is a method of isolating cellular components from at least one region of interest in a planar tissue section.

公开(公告)号：US10955315B2

公开(公告)日：2021-03-23

申请号：US15776791

申请日：2016-11-17

申请人： ETH Zurich

发明人： Thomas Templier ,  Richard Hahnloser

IPC分类号： B26D7/32 ,  G01N1/08 ,  G01N1/06 ,  G01N1/28 ,  G01N33/483

摘要： The invention relates to a method, a device, and a system for manipulating portions of a rigid body (1), and a computer program for the execution of the steps of the method for manipulating portions (20) of a rigid body (1). The method comprises the steps of providing a rigid body (1) comprising ultrathin-sectionable material, cutting an ultrathin portion (20) from the rigid body (1), providing the portion (20) at the surface of a liquid, providing at least one magnetic device (4), which produces a magnetic field or which is able to produce a magnetic field, using the magnetic field in order to provide a magnetic force, and using the magnetic force to move the portion (20) at the surface of the liquid. The device, the system and the computer program are adapted to carry out the method for manipulating portions (20) of a rigid body (1).

公开(公告)号：US20210073980A1

公开(公告)日：2021-03-11

申请号：US16950870

申请日：2020-11-17

申请人： 3Scan Inc.

发明人： Matthew Goodman ,  Todd Huffman ,  Cody Daniel

IPC分类号： G06T7/00 ,  G01N1/06 ,  G06K9/00 ,  G06T7/33 ,  G06K9/32

摘要： The following concerns a method for co-localization of microscopy or histology stains by the assembly of a virtual image from one or more imaging operations. In particular, the method decreases the time required to obtain multiple labeled antigen or protein histology images of a biological sample. The method includes imaging the tissue as it is sliced by a microtome with a knife edge scanning microscope and spatially aligning the samples by the generated images. The spatial alignment of samples enabled by the method allows a panel of different antigen or protein secondary or functional stains to be compared across different sample slices, thereby allowing concurrent secondary stains of tissues and cells.