利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

8 条记录 (耗时 0.013 秒)

    ION-SELECTIVE SENSORS
    1.
    发明申请
    ION-SELECTIVE SENSORS 审中-公开
    离子选择传感器

    公开(公告)号：WO2009051703A1

    公开(公告)日：2009-04-23

    申请号：PCT/US2008011726

    申请日：2008-10-14

    申请人： DRAPER LAB CHARLES S ,  CLARK HEATHER A ,  HARJES DANIEL I ,  DUBACH JOHN M

    发明人： CLARK HEATHER A ,  HARJES DANIEL I ,  DUBACH JOHN M

    IPC分类号： G01N33/58 ,  C12M1/34 ,  G01N21/64 ,  G01N27/333

    CPC分类号： G01N33/588 ,  B82Y5/00 ,  B82Y15/00 ,  G01N21/6458 ,  G01N27/3335 ,  G01N2021/6439

    摘要： The application provides on-selective sensors capable of selectively measuring ions, e.g., Na+, K+, Cl-, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and an ion-selective component such as an ionophore disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot or dye by directly absorbing its fluorescence emission.

    摘要翻译： 该应用提供能够选择性地测量单个活细胞的胞质溶胶中的离子，例如Na +，K +，Cl-等的选择性传感器。 传感器包括一个或多个量子点或荧光染料，pH敏感染料和离子选择性组分，例如设置在聚合物基质中的离子载体。 聚合物基质包括使得传感器能够定位在细胞的胞质溶胶内的内化部分。 内化部分包含在酸性条件下反应以从内体的范围释放传感器的小分子或肽，例如胺，antepennepedia，mastoparan或melittin。 一旦在细胞溶质中，传感器可以通过聚合物的选择性离子提取来检测离子分析物，从而在传感器内引起pH变化，从而改变pH敏感染料的吸光度。 吸光度的变化可以通过直接吸收其荧光发射来衰减来自量子点或染料的可检测发射强度，例如荧光强度。

    ION-SELECTIVE QUANTUM DOTS
    2.
    发明申请
    ION-SELECTIVE QUANTUM DOTS 审中-公开
    离子选择量子点

    公开(公告)号：WO2008016646A2

    公开(公告)日：2008-02-07

    申请号：PCT/US2007/017189

    申请日：2007-07-31

    申请人： THE CHARLES STARK DRAPER LABORATORY, INC. ,  CLARK, Heather, A. ,  HARJES, Daniel, I. ,  DUBACH, John, M.

    发明人： CLARK, Heather, A. ,  HARJES, Daniel, I. ,  DUBACH, John, M.

    CPC分类号： G01N33/588 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/5005 ,  G01N33/54313 ,  G01N33/6872 ,  G01N33/84 ,  G01N2333/726 ,  Y10S977/774

    摘要： A bipolar stimulator probe suitable for application of intramuscular stimulation provides an active stimulator electrode and a reference electrode as part of a single tool. The separation between the electrodes is significantly greater than the electrode separation of known bipolar stimulator probes and bipolar bar electrode configurations. The probe tool may be configured to permit adjustment of the fixed relative position of the electrodes within a given range. By providing a relatively wide spacing between the two electrodes, the tool can be used to approximate the effect of monopolar stimulation with a separate reference electrode, with the electrical stimulus producing less pain to the patient compared to bipolar stimulation where the two electrodes are spaced apart by only 2-4 cm or less.

    摘要翻译： 适用于肌内刺激的双极型刺激探针提供了一种主动刺激电极和参考电极，作为单一工具的一部分。 电极之间的间隔明显大于已知的双极性刺激探头和双极棒电极配置的电极分离。 探针工具可以被配置为允许在给定范围内调节电极的固定相对位置。 通过在两个电极之间提供相对宽的间隔，该工具可用于近似单极刺激与单独参考电极的影响，与双极刺激相比，电刺激对患者产生较少的疼痛，其中两个电极间隔开 只有2-4厘米或更小。

    QUANTUM DOT BASED FLUORESCENT ION-SENSOR
    3.
    发明申请
    QUANTUM DOT BASED FLUORESCENT ION-SENSOR 审中-公开
    基于量子的荧光离子传感器

    公开(公告)号：WO2008016646A3

    公开(公告)日：2008-07-24

    申请号：PCT/US2007017189

    申请日：2007-07-31

    申请人： DRAPER LAB CHARLES S ,  CLARK HEATHER A ,  HARJES DANIEL I ,  DUBACH JOHN M

    发明人： CLARK HEATHER A ,  HARJES DANIEL I ,  DUBACH JOHN M

    IPC分类号： G01N33/542 ,  C12Q1/00 ,  G01N33/543 ,  G01N33/68

    CPC分类号： G01N33/588 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/5005 ,  G01N33/54313 ,  G01N33/6872 ,  G01N33/84 ,  G01N2333/726 ,  Y10S977/774

    摘要： A bipolar stimulator probe suitable for application of intramuscular stimulation provides an active stimulator electrode and a reference electrode as part of a single tool. The separation between the electrodes is significantly greater than the electrode separation of known bipolar stimulator probes and bipolar bar electrode configurations. The probe tool may be configured to permit adjustment of the fixed relative position of the electrodes within a given range. By providing a relatively wide spacing between the two electrodes, the tool can be used to approximate the effect of monopolar stimulation with a separate reference electrode, with the electrical stimulus producing less pain to the patient compared to bipolar stimulation where the two electrodes are spaced apart by only 2-4 cm or less.

    摘要翻译： 适用于肌内刺激的双极型刺激探针提供了一种主动刺激电极和参考电极，作为单一工具的一部分。 电极之间的间隔明显大于已知的双极性刺激探头和双极棒电极配置的电极分离。 探针工具可以被配置为允许在给定范围内调节电极的固定相对位置。 通过在两个电极之间提供相对宽的间隔，该工具可用于近似单分子刺激与单独的参比电极的影响，与双极刺激相比，电刺激对患者产生较少的疼痛，其中两个电极间隔开 只有2-4厘米或更小。

    ION-SELECTIVE SENSORS
    5.
    发明申请
    ION-SELECTIVE SENSORS 审中-公开

    公开(公告)号：WO2009051703A8

    公开(公告)日：2009-04-23

    申请号：PCT/US2008/011726

    申请日：2008-10-14

    申请人： THE CHARLES STARK DRAPER LABORATORY, INC. ,  CLARK, Heather, A. ,  HARJES, Daniel, I. ,  DUBACH, John, M.

    发明人： CLARK, Heather, A. ,  HARJES, Daniel, I. ,  DUBACH, John, M.

    IPC分类号： G01N33/58 ,  G01N21/64 ,  C12M1/34 ,  G01N27/333

    摘要： The application provides ion-selective sensors capable of selectively measuring ions, e.g., Na + , K + , Cl - , etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and an ion-selective component such as an ionophore disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot or dye by directly absorbing its fluorescence emission.