公开(公告)号：WO2007131354A1

公开(公告)日：2007-11-22

申请号：PCT/CA2007/000857

申请日：2007-05-11

Applicant: UNIVERSITE LAVAL ,  NAJARI, Ahmed ,  HO, Hoang-Anh ,  LECLERC, Mario

Inventor： NAJARI, Ahmed ,  HO, Hoang-Anh ,  LECLERC, Mario

IPC: C40B40/06 ,  C07H21/00 ,  C12Q1/68 ,  C40B30/04 ,  C40B50/14 ,  C40B60/12 ,  C40B70/00 ,  G01N33/52 ,  G01N33/53

CPC classification number: G01N33/54306 ,  B01J19/0046 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00644 ,  B01J2219/00659 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2565/101

Abstract: Methods and tools (e.g., kits, articles of manufacturing, support and arrays) for the solid-phase detection of a target molecule using a cationic polymer and nucleic acid probe complex is provided herewith. These methods and tools allows for the reagentless, ultrasensitive and specific detection of nucleic acids, proteins and other molecules of interest and are based on a labeled complex made of specific capture probes and a polythiophene derivative.

Abstract translation: 本文提供了使用阳离子聚合物和核酸探针复合物对靶分子的固相检测的方法和工具（例如，试剂盒，制品，载体和阵列）。 这些方法和工具允许核酸，蛋白质和其他感兴趣的分子的无试剂，超灵敏和特异性检测，并且基于由特异性捕获探针和聚噻吩衍生物制成的标记复合物。

公开(公告)号：WO2006092063A1

公开(公告)日：2006-09-08

申请号：PCT/CA2006/000322

申请日：2006-03-03

Applicant: NATIONAL RESEARCH COUNCIL CANADA ,  LECLERC, Mario ,  HO, Hoang-Anh

Inventor： LECLERC, Mario ,  HO, Hoang-Anh

IPC: C12Q1/68 ,  C12Q1/00

CPC classification number: C12Q1/6816 ,  C12Q1/682 ,  Y10T436/143333 ,  C12Q2565/113 ,  C12Q2565/107 ,  C12Q2563/107

Abstract: The present invention relates to a novel integrated PCR-free signal amplification polynucleotide detection system which combines a specific receptor, an optical transducer, and an amplification mechanism. This novel detection system is based on different electrostatic interactions and conformations between a cationic polythiophene (i.e. polymer 1) and single-stranded or double-stranded polynucleotides (such as ss-DNA or ds-DNA), and the efficient energy transfer between the triplex (complexation between the cationic polythiophene and ds-DNA) and neighboring fluorophores attached to ss-DNA or ds-DNA probes. It is to be understood that in the case of ss-DNA, triplex formation occurs via the hybridization of complementary ss-DNA strands, combined with complexation with the cationic polythiophene. The present detection system allows for the detection of single nucleotide polymorphisms (SNPs) from samples in only a few minutes, without the need for nucleic acid amplification.

Abstract translation: 本发明涉及一种结合特异性受体，光学换能器和放大机制的新型无PCR信号整合多聚核苷酸检测系统。 这种新型检测系统是基于阳离子聚噻吩（即聚合物1）和单链或双链多核苷酸（如ss-DNA或ds-DNA）之间的不同静电相互作用和构象，以及三链体之间的有效能量转移 （阳离子聚噻吩和ds-DNA之间的络合）和与ss-DNA或ds-DNA探针相连的相邻荧光团。 应当理解，在ss-DNA的情况下，通过互补的ss-DNA链的杂交与阳离子聚噻吩的络合结合发生三链体形成。 本检测系统允许仅在几分钟内从样品中检测单核苷酸多态性（SNPs），而不需要核酸扩增。

公开(公告)号：WO2002081735A2

公开(公告)日：2002-10-17

申请号：PCT/CA2002/000485

申请日：2002-04-05

Applicant: INFECTIO DIAGNOSTIC (I.D.I.), INC. ,  LECLERC, Mario ,  HO, Hoang,Anh ,  BOISSINOT, Maurice

Inventor： LECLERC, Mario ,  HO, Hoang,Anh ,  BOISSINOT, Maurice

IPC: C12Q1/68

CPC classification number: C07D409/12 ,  C07D333/32 ,  C07D409/06 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q2563/137

Abstract: Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly (3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hibridized) oligonucleotides.

Abstract translation: 公开了允许双链寡核苷酸的简单光学和电化学检测的新方法。 这些方法是快速，有选择性和多功能的。 有利的是，它们不需要在探针或分析物上进行任何化学反应，因为它们是基于阳离子聚（3-烷氧基-4-甲基噻吩）衍生物和单链或双链（杂化）寡核苷酸之间的不同静电相互作用。

公开(公告)号：WO2009009889A1

公开(公告)日：2009-01-22

申请号：PCT/CA2008/001299

申请日：2008-07-11

Applicant: NATIONAL RESEARCH COUNCIL OF CANADA ,  DUBUS, Sébastien ,  HO, Hoang-Anh ,  LECLERC, Mario ,  BOUDREAU, Denis

Inventor： DUBUS, Sébastien ,  HO, Hoang-Anh ,  LECLERC, Mario ,  BOUDREAU, Denis

IPC: C12Q1/68 ,  C08G61/12 ,  C08L65/00 ,  C09D165/00 ,  C40B30/04 ,  C40B70/00

CPC classification number: C08G61/126 ,  C12Q1/682 ,  C40B30/04 ,  C40B70/00 ,  C12Q2565/113

Abstract: The invention relates to methods and reagents for detecting minute amounts of targets having affinity for nucleic acid. The present invention more particularly relates to target detection using aggregates of cationic polymer chains and nucleic acid capture probes linked to particles, such as controllable mobility particles.

Abstract translation: 本发明涉及用于检测对核酸具有亲和性的微量目标物的方法和试剂。 本发明更具体地涉及使用阳离子聚合物链的聚集体和连接到颗粒如可控迁移率颗粒的核酸捕获探针的靶检测。

公开(公告)号：WO2004106544A2

公开(公告)日：2004-12-09

申请号：PCT/CA2004/000824

申请日：2004-06-03

Applicant: INFECTIO DIAGNOSTIC (I.D.I) INC. ,  UNIVERSITE LAVAL ,  LECLERC, Mario ,  HO, Hoang-Anh ,  BOISSINOT, Maurice

Inventor： LECLERC, Mario ,  HO, Hoang-Anh ,  BOISSINOT, Maurice

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q2563/173 ,  C12Q2563/107 ,  C12Q2537/101 ,  C12Q2525/205

Abstract: An optical sensor for detecting a target comprising a single­stranded aptamer complementary to said target, and a water-soluble cationic polythiophene derivative of the following formula: wherein "n" is an integer ranging from 6 to 100, is disclosed. The optical sensor allows for the detection of targets selected from the group consisting of potassium ions, small organic molecules, amino acids, proteins, whole cells and nucleotides. The detection is based on the formation of hybrid anionic aptamer/cationic polythiophene complexes.

Abstract translation: 一种用于检测包含与所述靶互补的单链适配体的靶的光学传感器和下式的水溶性阳离子聚噻吩衍生物：其中“n”为6至100的整数。 光学传感器允许检测从由钾离子，小有机分子，氨基酸，蛋白质，全细胞和核苷酸组成的组中选择的靶标。 检测是基于杂交阴性适体/阳离子聚噻吩复合物的形成。

公开(公告)号：WO02081735A3

公开(公告)日：2003-04-17

申请号：PCT/CA0200485

申请日：2002-04-05

Applicant: INFECTIO DIAGNOSTIC INC ,  LECLERC MARIO ,  HO HOANG ANH ,  BOISSINOT MAURICE

Inventor： LECLERC MARIO ,  HO HOANG ANH ,  BOISSINOT MAURICE

IPC: G01N33/53 ,  C07D333/20 ,  C07D333/32 ,  C07D333/42 ,  C07D409/06 ,  C07D409/12 ,  C08G61/12 ,  C08G75/32 ,  C12Q1/68 ,  G01N21/78 ,  G01N27/48 ,  G01N33/566 ,  G01N33/58

CPC classification number: C07D409/12 ,  C07D333/32 ,  C07D409/06 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q2563/137

Abstract: Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly (3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hibridized) oligonucleotides.

Abstract translation: 公开了允许双链寡核苷酸的简单光学和电化学检测的新方法。 这些方法是快速，有选择性和多功能的。 有利的是，它们不需要在探针或分析物上进行任何化学反应，因为它们是基于阳离子聚（3-烷氧基-4-甲基噻吩）衍生物和单链或双链（杂化）寡核苷酸之间的不同静电相互作用。

公开(公告)号：WO2004106544A3

公开(公告)日：2005-04-21

申请号：PCT/CA2004000824

申请日：2004-06-03

Applicant: INFECTIO DIAGNOSTIC INC ,  UNIV LAVAL ,  LECLERC MARIO ,  HO HOANG-ANH ,  BOISSINOT MAURICE

Inventor： LECLERC MARIO ,  HO HOANG-ANH ,  BOISSINOT MAURICE

IPC: C12Q1/68 ,  C07D333/32 ,  C12N15/11

CPC classification number: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q2563/173 ,  C12Q2563/107 ,  C12Q2537/101 ,  C12Q2525/205

Abstract: An optical sensor for detecting a target comprising a singlestranded aptamer complementary to said target, and a water-soluble cationic polythiophene derivative of the following formula (I) wherein "n" is an integer ranging from 6 to 100, is disclosed. The optical sensor allows for the detection of targets selected from the group consisting of potassium ions, small organic molecules, amino acids, proteins, whole cells and nucleotides. The detection is based on the formation of hybrid anionic aptamer/cationic polythiophene complexes.

Abstract translation: 一种用于检测包含与所述靶互补的单链适配体的靶的光学传感器和下式（I）的水溶性阳离子聚噻吩衍生物，其中“n”为6至100的整数。 光学传感器允许检测从由钾离子，小有机分子，氨基酸，蛋白质，全细胞和核苷酸组成的组中选择的靶标。 检测是基于杂交阴性适体/阳离子聚噻吩复合物的形成。