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    UNIVERSAL METHOD AND COMPOSITION FOR THE RAPID LYSIS OF CELLS FOR THE RELEASE OF NUCLEIC ACIDS AND THEIR DETECTION
    1.
    发明申请
    UNIVERSAL METHOD AND COMPOSITION FOR THE RAPID LYSIS OF CELLS FOR THE RELEASE OF NUCLEIC ACIDS AND THEIR DETECTION 审中-公开
    用于释放核酸的细胞快速裂解的通用方法和组合物及其检测

    公开(公告)号：WO03008636A2

    公开(公告)日：2003-01-30

    申请号：PCT/CA0201088

    申请日：2002-07-19

    Applicant: INFECTIO DIAGNOSTIC INC ,  MENARD CHRISTIAN ,  PICARD FRANCOIS J

    Inventor： MENARD CHRISTIAN ,  PICARD FRANCOIS J

    IPC: C07H21/04 ,  C12N1/06 ,  C12N15/10 ,  C12Q1/68

    CPC classification number: C07H21/04 ,  C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/137 ,  C12Q2527/125

    Abstract: This invention describes a rapid (10 to 15 minutes), simple, flexible and efficient method of nucleic acids extraction for nucleic acid testing assays. This method has the following basic steps: i) mechanical cell lysis using solid particles in the presence of a chelating agent, followed by ii) controlling the presence and/or activity of NAT assays inhibitors. This method is applicable to various biological samples and universal for microorganisms, as one can use it to extract nucleic acids from test samples containing target viruses, bacteria, bacterial spores, fungi, parasites or other eukaryotic cells, including animal and human cells.

    Abstract translation: 本发明描述了用于核酸测试测定的核酸提取的快速（10至15分钟），简单，灵活且有效的方法。 该方法具有以下基本步骤：i）在螯合剂存在下使用固体颗粒进行机械细胞裂解，然后ii）控制NAT测定抑制剂的存在和/或活性。 这种方法适用于各种生物样品，通用于微生物，因为人们可以用它从含有靶病毒，细菌，细菌孢子，真菌，寄生虫或其他真核细胞，包括动物和人类细胞的测试样品中提取核酸。

    BIOLOGICAL REAGENTS AND METHODS TO VERIFY THE EFFICIENCY OF SAMPLE PREPARATION AND NUCLEIC ACID AMPLIFICATION AND/OR DETECTION
    2.
    发明申请
    BIOLOGICAL REAGENTS AND METHODS TO VERIFY THE EFFICIENCY OF SAMPLE PREPARATION AND NUCLEIC ACID AMPLIFICATION AND/OR DETECTION 审中-公开
    生物试剂和方法来验证样品制备和核酸扩增和/或检测的效率

    公开(公告)号：WO2004055205A3

    公开(公告)日：2004-08-26

    申请号：PCT/CA0301925

    申请日：2003-12-15

    Applicant: INFECTIO DIAGNOSTIC INC ,  GAYRAL JEAN-PIERRE ,  PICARD FRANCOIS ,  BOISSINOT MAURICE ,  BASTIEN MARTINE

    Inventor： GAYRAL JEAN-PIERRE ,  PICARD FRANCOIS ,  BOISSINOT MAURICE ,  BASTIEN MARTINE

    IPC: C12Q1/68

    CPC classification number: C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q2545/101

    Abstract: This invention relates to reagent comprising: any one of cells, viral particles, organelles, parasites, cells comprising organelles, cells comprising viral particles, cells comprising parasites, cells comprising bacterial cells and any combination thereof, the cells, viral particles, organelles or parasites comprising at least one nucleic acid sequence serving as an internal control (IC) target for nucleic acid testing (NAT) assay; wherein the reagent is suitable to be added to a test sample undergoing sample preparation to release, concentrate and/or purify nucleic acids and amplification and/or detection of nucleic acids so as to be used to verify: (i) the efficiency of sample preparation; and (ii) the efficiency of nucleic acid amplification and/or detection. The present invention also relates to a method to verify or validate the preparation and amplification and/or detection of a nucleic acid target sequence in a sample spiked with a reagent of the present invention.

    Abstract translation: 本发明涉及试剂，其包含：细胞，病毒颗粒，细胞器，寄生虫，包含细胞器的细胞，包含病毒颗粒的细胞，包含寄生虫的细胞，包含细菌细胞的细胞及其任何组合，细胞，病毒颗粒，细胞器或寄生虫 包括用作核酸测试（NAT）测定的内部对照（IC）靶的至少一个核酸序列; 其中所述试剂适于加入到经过样品制备以测试核酸的测试样品中以及核酸的扩增和/或检测，以便用于验证：（i）样品制备的效率 ; 和（ii）核酸扩增和/或检测的效率。 本发明还涉及验证或验证掺有本发明试剂的样品中核酸靶序列的制备和扩增和/或检测的方法。

    Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
    7.
    发明申请
    Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories 审中-公开
    物种特异性，属特异性和通用DNA探针和扩增引物，用于快速检测和鉴定临床标本中常见的细菌和真菌病原体和相关抗生素抗性基因，用于微生物实验室的诊断

    公开(公告)号：WO9820157A3

    公开(公告)日：1998-08-13

    申请号：PCT/CA9700829

    申请日：1997-11-04

    Applicant: INFECTIO DIAGNOSTIC INC ,  BERGERON MICHEL G ,  PICARD FRANCOIS J ,  OUELETTE MARC ,  ROY PAUL H

    Inventor： BERGERON MICHEL G ,  PICARD FRANCOIS J ,  OUELLETTE MARC ,  ROY PAUL H

    IPC: C12N15/09 ,  C07K14/195 ,  C07K14/21 ,  C07K14/245 ,  C07K14/26 ,  C07K14/285 ,  C07K14/31 ,  C07K14/315 ,  C12N1/21 ,  C12Q1/68 ,  C12R1/01

    CPC classification number: C12Q1/689 ,  C07K14/195 ,  C07K14/21 ,  C07K14/212 ,  C07K14/245 ,  C07K14/26 ,  C07K14/285 ,  C07K14/31 ,  C07K14/315 ,  C07K14/3156 ,  C12Q1/6895 ,  C12Q2600/156

    Abstract: DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample DNA from (i) any bacterium, (ii) the species Streptococcus agalactiae, Staphylococcus saprophyticus, Enterococcus faecium, Neisseria meningitidis, Listeria monocytogenes and Candida albicans, and (iii) any species of the genera Streptococcus, Staphylococcus, Enterococcus, Neisseria and Candida are disclosed. DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample antibiotic resistance genes selected from the group consisting of blatem, blarob, blashv, blaoxa, blaZ, aadB, aacC1, aacC2, aacC3, aacA4, aac6'-IIa, ermA, ermB, ermC, mecA, vanA, vanB, vanC, satA, aac(6'-aph(2''), aad(6'), vat, vga, msrA, sul and int are also disclosed. The above microbial species, genera and resistance genes are all clinically relevant and commonly encountered in a variety of clinical specimens. These DNA-based assays are rapid, accurate and can be used in clinical microbiology laboratories for routine diagnosis. These novel diagnostic tools should be useful to improve the speed and accuracy of diagnosis of microbial infections, thereby allowing more effective treatments. Diagnostic kits for (i) the universal detection and quantification of bacteria, and/or (ii) the detection, identification and quantification of the above-mentioned bacterial and fungal species and/or genera, and/or (iii) the detection, identification and quantification of the above-mentioned antibiotic resistance genes are also claimed.

    Abstract translation: 基于DNA的方法，使用扩增引物或探针来检测，鉴定和定量来自（i）任何细菌的测试样品DNA，（ii）无乳链球菌，腐生葡萄球菌，屎肠球菌，脑膜炎奈瑟氏球菌，单核细胞增生利斯特氏菌和白色念珠菌 ，和（iii）任何种属的链球菌属，葡萄球菌属，肠球菌属，奈瑟球菌属和假丝酵母属。 基于DNA的方法，使用扩增引物或探针来检测，鉴定和定量测试样品抗生素抗性基因，其选自bla tem，bla rob，bla shv，bla oxa，blaZ，aadB，aacC1，aacC2，aacC3 ，aacA4，aac6'-IIa，ermA，ermB，ermC，mecA，vanA，vanB，vanC，satA，aac（6'） - aph（2“），aad（6'），vat，vga，msrA， 上述微生物种类，属和抗性基因全部临床相关，常见于各种临床标本，这些基于DNA的检测方法快速，准确，可用于临床微生物实验室进行常规诊断。 诊断工具可用于提高微生物感染诊断的速度和准确性，从而允许更有效的治疗。诊断试剂盒用于（i）细菌的普遍检测和定量，和/或（ii）检测，鉴定和定量 的上述细菌 和/或类，和/或（iii）上述抗生素抗性基因的检测，鉴定和定量也被要求保护。

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