公开(公告)号：WO2012061444A2

公开(公告)日：2012-05-10

申请号：PCT/US2011058857

申请日：2011-11-01

Applicant: HIDDESSEN AMY L ,  MASQUELIER DONALD A ,  NESS KEVIN D ,  HINDSON BENJAMIN J ,  MAKAREWICZ ANTHONY J JR ,  STEENBLOCK ERIN R

Inventor： HIDDESSEN AMY L ,  MASQUELIER DONALD A ,  NESS KEVIN D ,  HINDSON BENJAMIN J ,  MAKAREWICZ ANTHONY J JR ,  STEENBLOCK ERIN R

IPC: F17D1/16 ,  F03B11/02

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F13/1022 ,  B01L3/502761 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0867 ,  B01L2400/0487 ,  G01N1/38 ,  G01N2035/1046 ,  Y10T137/0396 ,  Y10T137/8593

Abstract: System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.

Abstract translation: 系统，包括用于形成乳液的方法，设备和试剂盒。 该系统可以包括由仪器接收的仪器和微流体芯片。 该仪器可以对由芯片保持的预期乳剂阶段施加压力，以驱动芯片中乳剂的形成和收集。 在一些实施例中，当仪器检测到满足预定条件的压力变化时，仪器可以停止对芯片施加压力。 改变可能表明已经达到了液滴产生的终点。

公开(公告)号：WO2014205447A3

公开(公告)日：2015-03-12

申请号：PCT/US2014043715

申请日：2014-06-23

Applicant: BIO RAD LABORATORIES ,  MAKAREWICZ ANTHONY J JR ,  BOUSSE LUC ,  SCHIAFFINO STEFANO

Inventor： MAKAREWICZ ANTHONY J JR ,  BOUSSE LUC ,  SCHIAFFINO STEFANO

IPC: G01N1/00

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/04 ,  B01L2300/041 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  B01L2400/0677 ,  B01L2400/084 ,  C12N15/1075 ,  C12Q1/6806 ,  Y10T436/2575

Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In some embodiments, the system may include a well and a channel component attached to the well. The channel component may include (a) a body, (b) an input tube (a "fluid pickup") projecting from a bottom surface of the body and having an open bottom end disposed in the input well, (c) a microchannel, and (d) a passage extending through the input tube and the body and connecting the well to the microchannel. The system may be configured to receive a sample- containing fluid in the well and retain the sample-containing fluid below a top end of the passage, until a pressure differential is created that drives at least a portion of the sample-containing fluid from the well via the passage and through the microchannel.

Abstract translation: 微流体系统，包括用于处理流体的方法和装置，例如通过液滴产生。 在一些实施例中，系统可以包括井和连接到井的通道部件。 通道部件可以包括（a）主体，（b）从主体的底表面突出并具有设置在输入孔中的敞开的底端的输入管（“流体拾取器”），（c）微通道， 和（d）延伸穿过输入管和主体并将井连接到微通道的通道。 该系统可以被配置为在井中接收含样品的流体并将含样品的流体保持在通道的顶端下方，直到形成压力差，从而将含有样品的流体的至少一部分从 通过通道和通过微通道。

公开(公告)号：WO2019006190A8

公开(公告)日：2019-01-03

申请号：PCT/US2018/040125

申请日：2018-06-28

Applicant: BIO-RAD LABORATORIES, INC. ,  STUMBO, David, P. ,  CARMAN, George ,  HOBBS, Steve ,  MAKAREWICZ, Anthony, J., Jr. ,  SIMONIAN, Dmitri ,  GLADE, David ,  OEN, Joshua ,  PRISTINSKI, Denis ,  DZENITIS, John

Inventor： STUMBO, David, P. ,  CARMAN, George ,  HOBBS, Steve ,  MAKAREWICZ, Anthony, J., Jr. ,  SIMONIAN, Dmitri ,  GLADE, David ,  OEN, Joshua ,  PRISTINSKI, Denis ,  DZENITIS, John

IPC: B01L3/02 ,  G01N35/10

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：WO2014205447A2

公开(公告)日：2014-12-24

申请号：PCT/US2014/043715

申请日：2014-06-23

Applicant: BIO-RAD LABORATORIES, INC. ,  MAKAREWICZ, Anthony, J., Jr. ,  BOUSSE, Luc ,  SCHIAFFINO, Stefano

Inventor： MAKAREWICZ, Anthony, J., Jr. ,  BOUSSE, Luc ,  SCHIAFFINO, Stefano

IPC: B01L3/00 ,  C12N15/10

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/04 ,  B01L2300/041 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  B01L2400/0677 ,  B01L2400/084 ,  C12N15/1075 ,  C12Q1/6806 ,  Y10T436/2575

Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In some embodiments, the system may include a well and a channel component attached to the well. The channel component may include (a) a body, (b) an input tube (a "fluid pickup") projecting from a bottom surface of the body and having an open bottom end disposed in the input well, (c) a microchannel, and (d) a passage extending through the input tube and the body and connecting the well to the microchannel. The system may be configured to receive a sample- containing fluid in the well and retain the sample-containing fluid below a top end of the passage, until a pressure differential is created that drives at least a portion of the sample-containing fluid from the well via the passage and through the microchannel.

Abstract translation: 微流体系统，包括用于处理流体的方法和装置，例如通过液滴产生。 在一些实施例中，系统可以包括井和连接到井的通道部件。 通道部件可以包括（a）主体，（b）从主体的底表面突出并具有设置在输入孔中的敞开的底端的输入管（“流体拾取器”），（c）微通道， 和（d）延伸穿过输入管和主体并将井连接到微通道的通道。 该系统可以被配置为在井中接收含样品的流体并将含样品的流体保持在通道的顶端下方，直到形成压力差，从而将含有样品的流体的至少一部分从 通过通道和通过微通道。

公开(公告)号：WO2012061444A3

公开(公告)日：2012-05-10

申请号：PCT/US2011/058857

申请日：2011-11-01

Applicant: HIDDESSEN, Amy L. ,  MASQUELIER, Donald A. ,  NESS, Kevin D. ,  HINDSON, Benjamin J. ,  MAKAREWICZ, Anthony J., Jr. ,  STEENBLOCK, Erin R.

Inventor： HIDDESSEN, Amy L. ,  MASQUELIER, Donald A. ,  NESS, Kevin D. ,  HINDSON, Benjamin J. ,  MAKAREWICZ, Anthony J., Jr. ,  STEENBLOCK, Erin R.

IPC: B01F15/00

Abstract: System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.