公开(公告)号：WO2013022799A1

公开(公告)日：2013-02-14

申请号：PCT/US2012/049646

申请日：2012-08-03

Applicant: DANISCO US INC. ,  BERGSMA, Martien, H. ,  CALABRIA, Anthony, R. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline, Johanna Maria ,  XU, Hongxian

Inventor： BERGSMA, Martien, H. ,  CALABRIA, Anthony, R. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline, Johanna Maria ,  XU, Hongxian

IPC: C12P5/00 ,  C12N9/24

CPC classification number: C12P5/007 ,  C12N9/1085 ,  C12N9/2428 ,  C12Y205/0101 ,  C12Y302/01003

Abstract: Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

Abstract translation: 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产类异戊二烯前体分子和/或类异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。

公开(公告)号：WO2010132157A2

公开(公告)日：2010-11-18

申请号：PCT/US2010/030443

申请日：2010-04-08

Applicant: DANISCO US INC. ,  DUAN, Gang ,  QIAN, Ying ,  SALA, Rafael, F. ,  SHETTY, Jayarama

Inventor： DUAN, Gang ,  QIAN, Ying ,  SALA, Rafael, F. ,  SHETTY, Jayarama

IPC: C12P19/22

CPC classification number: C12P19/14

Abstract: The combination of a Pseudomonas saccharophila G4-forming amylase (PS4) variant and a pullulanase advantageously can catalyze at a high temperature saccharification to produce an increased amount of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant supplement with a pullulanase is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.

Abstract translation: 酿酒酵母G4形成淀粉酶（PS4）变体和支链淀粉酶的组合有利地可以在高温糖化下催化产生增加量的麦芽四糖，其可以在生产麦芽四糖糖浆的方法中用于下游。 在一个实施方案中，提供了具有支链淀粉酶的热稳定性PS4变体，其可以基于总糖含量产生约40重量％至约60重量％的麦芽四糖。

公开(公告)号：WO2008112729A2

公开(公告)日：2008-09-18

申请号：PCT/US2008/056601

申请日：2008-03-12

Applicant: DANISCO US INC., GENENCOR DIVISION ,  DUAN, Gang ,  QIAN, Kathy ,  SCHEFFERS, Martijn ,  SHETTY, Jayarama ,  VAN SOLINGEN, Pieter

Inventor： DUAN, Gang ,  QIAN, Kathy ,  SCHEFFERS, Martijn ,  SHETTY, Jayarama ,  VAN SOLINGEN, Pieter

IPC: C07K14/19

CPC classification number: C12N9/242

Abstract: A maltogenic a-amylase from Trichoderma reesei (TrAA) and variants thereof are useful in the production of high-maltose syrups from liquefied starch. Particularly high maltose concentrations are achieved when TrAA is used in the presence of a pullulanase. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.

Abstract translation: 来自里氏木霉Trichoderma reesei（TrAA）的产麦芽糖α-淀粉酶及其变体可用于从液化淀粉生产高麦芽糖糖浆。 当在支链淀粉酶存在下使用TrAA时，可以获得特别高的麦芽糖浓度。 还提供了用于生产TrAA及其变体的表达宿主和编码核酸。

公开(公告)号：WO2017116840A1

公开(公告)日：2017-07-06

申请号：PCT/US2016/067727

申请日：2016-12-20

Applicant: DANISCO US INC. ,  DUAN, Gang ,  GOHEL, Vipul ,  LIU, Xuan ,  QIAN, Ying ,  RANGANATHAN, Kannan ,  ZHANG, Xiaoping

Inventor： DUAN, Gang ,  GOHEL, Vipul ,  LIU, Xuan ,  QIAN, Ying ,  RANGANATHAN, Kannan ,  ZHANG, Xiaoping

IPC: C12P19/00 ,  C12P7/06 ,  C12P7/08 ,  C12P7/10

CPC classification number: C12P7/06 ,  C12P7/08 ,  C12P7/10 ,  C12P19/00 ,  Y02E50/16 ,  Y02E50/17

Abstract: Methods and compositions for enhanced production of at least one fermentation product resulting from the fermentation of sugar containing medium obtained from one or more feedstocks are disclosed herein. The compositions disclosed herein comprise trehalase.

Abstract translation: 本文公开了用于增强生产由从一种或多种原料获得的含糖培养基的发酵产生的至少一种发酵产物的方法和组合物。 本文公开的组合物包含海藻糖酶。

公开(公告)号：WO2013148207A3

公开(公告)日：2013-10-03

申请号：PCT/US2013/030980

申请日：2013-03-13

Applicant: DANISCO US INC.

Inventor： BLAKE, Collette Michelle ,  DUAN, Gang ,  KO, Douglas ,  KOOY, Floor K. ,  LEE, Sung, Ho ,  PEPSIN, Michael J. ,  QIAN, Ying ,  REBOLI, Matthew T. ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Paula Johanna, Maria ,  XU, Hongxian

IPC: C12P19/12

Abstract: Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

公开(公告)号：WO2012019159A1

公开(公告)日：2012-02-09

申请号：PCT/US2011/046849

申请日：2011-08-05

Applicant: DANISCO US INC. ,  BERGSMA, Martien, H. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung, Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline Johanna, Maria ,  XU, Hongxian

Inventor： BERGSMA, Martien, H. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung, Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline Johanna, Maria ,  XU, Hongxian

IPC: C12P7/06

CPC classification number: C12P7/06 ,  C12P7/04 ,  C12P7/14 ,  C12P7/16 ,  C12P7/18 ,  C12P7/46 ,  C12P7/56 ,  C12P13/04 ,  C12P13/14 ,  C12P19/20 ,  Y02E50/10 ,  Y02E50/17

Abstract: Embodiments of the present disclosure relate to a process for producing downstream products, such as fermentable sugars and end products, from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

Abstract translation: 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产下游产物如可发酵糖和终产物的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。

公开(公告)号：WO2018226569A1

公开(公告)日：2018-12-13

申请号：PCT/US2018/035825

申请日：2018-06-04

Applicant: DANISCO US INC

Inventor： GOHEL, Vipul ,  KANNAN, Rangana Than ,  QIAN, Kathy ,  DUAN, Gang

IPC: C12P19/00 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14 ,  C12P19/16 ,  C12N9/96

CPC classification number: C12P19/16 ,  C12N9/96 ,  C12P19/02 ,  C12P19/14

Abstract: Described are methods relating to the use of low concentrations of betaine to increase the activity and/or stability of enzymes in stressful environments, such as in starch liquefaction and/or sachharification.

公开(公告)号：WO2015066669A1

公开(公告)日：2015-05-07

申请号：PCT/US2014/063798

申请日：2014-11-04

Applicant: DANISCO US INC.

Inventor： SHARMA, Vivek ,  TEUNISSEN, Paula Johanna Maria ,  CHIN, Regina ,  BLAKE, Collette Michelle ,  LEE, Sang-Kyu ,  QIAN, Ying ,  LIU, Xuan ,  DUAN, Gang ,  XU, Hongxian

IPC: C12P19/14 ,  C12P7/06 ,  C12P7/40

CPC classification number: C12P7/56 ,  C12P7/06 ,  C12P7/48 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/17

Abstract: The present invention relates to reducing the viscosity of a corn-like grain slurry during a liquefaction and/or fermentation. The invention also relates to increasing the rate and/or yield of fermentation products, including, but not limited to ethanol, lactic acid and citric acid from corn-like grains.

Abstract translation: 本发明涉及在液化和/或发酵期间降低玉米样颗粒浆料的粘度。 本发明还涉及增加发酵产物的速率和/或产率，包括但不限于来自玉米状颗粒的乙醇，乳酸和柠檬酸。

公开(公告)号：WO2015061135A1

公开(公告)日：2015-04-30

申请号：PCT/US2014/060934

申请日：2014-10-16

Applicant: DANISCO US INC.

Inventor： DUAN, Gang ,  PETERSEN, Lars W. ,  QIAN, Ying ,  SHETTY, Jayarama K. ,  WILSON, Troy ,  XU, Hongxian ,  ZHANG, Xiaoping

IPC: A23L1/30 ,  A23L1/308

CPC classification number: A23L33/21 ,  A23L29/244 ,  A23L33/135 ,  A23V2002/00 ,  A23Y2220/67 ,  A23Y2220/73 ,  A23V2200/3202 ,  A23V2200/3204 ,  A23V2250/28 ,  A23V2250/5062 ,  A23V2250/62

Abstract: The present teachings provide a method of forming an edible composition, and the compositions themselves. The method generally involves providing a complex functional oligosaccharide to a fermenting microorganism to produce, via fermentation, a low complexity functional oligosaccharide and a desirable biochemical. In recovering the low complexity functional oligosaccharide and the desirable biochemical, the microorganism is not separated. This results in an easy to form edible composition.

Abstract translation: 本教导提供了形成可食用组合物的方法，以及组合物本身。 该方法通常涉及向发酵微生物提供复合功能性寡糖，以通过发酵生产低复杂度功能性寡糖和期望的生物化学品。 在回收低复杂度的功能寡糖和所需的生物化学物质时，微生物不分离。 这导致容易形成可食用组合物。

公开(公告)号：WO2012027226A1

公开(公告)日：2012-03-01

申请号：PCT/US2011/048448

申请日：2011-08-19

Applicant: DANISCO US INC. ,  DUAN, Gang ,  JACOBSEN, Kathleen Burns ,  SHETTY, Jayarama K. ,  YING, Qian ,  WILSON, Troy

Inventor： DUAN, Gang ,  JACOBSEN, Kathleen Burns ,  SHETTY, Jayarama K. ,  YING, Qian ,  WILSON, Troy

IPC: A23L1/305 ,  A23J1/12 ,  A23J3/14 ,  A23J3/34 ,  A23K1/00 ,  A23K1/16 ,  A23G3/54 ,  A23G1/54

CPC classification number: A23J3/346 ,  A23G1/54 ,  A23G3/54 ,  A23J1/125 ,  A23J3/14 ,  A23K20/147 ,  A23L33/185 ,  A23V2002/00 ,  C12Y302/01001 ,  C12Y302/01003 ,  A23V2250/548

Abstract: Rice protein concentrates prepared at low temperature exhibit improved functionality and beneficial physiological benefits, including lowered cholesterol and enhanced lactic acid dehydrogenase activity, without an increase in blood urea nitrogen. The rice protein concentration could be made into a wet dough with comparatively less water than a soy protein concentrate. Use of the rice protein concentrate thus improved processing steps in the formulation of a food article containing the concentrate. The food product advantageously shows an extending shelf life and improved palatable texture.

Abstract translation: 在低温下制备的大米蛋白浓缩物显示改善的功能和有益的生理益处，包括降低的胆固醇和提高的乳酸脱氢酶活性，而不增加血液尿素氮。 大米蛋白质浓度可以制成湿面团，水分比大豆蛋白浓缩物少。 大米蛋白浓缩物的使用从而改善了含有浓缩物的食品制剂的加工步骤。 食品有利地显示出延长的保质期和改善的可口的质感。