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公开(公告)号：WO2017112643A1
公开(公告)日：2017-06-29
申请号：PCT/US2016/067708
申请日：2016-12-20
申请人： DANISCO US INC. , KOOPS, Bart C. , TEUNISSEN, Paula Johanna Maria , VAN BRUSSEL, Marco , SCHEFFERS, Martijn , GUIJT, Kees-Jan , ZOU, Zhengzheng , TANG, Zhongmei , QIAN, Zhen , GE, Jing , ZHANG, Zhenghong
发明人： KOOPS, Bart C. , TEUNISSEN, Paula Johanna Maria , VAN BRUSSEL, Marco , SCHEFFERS, Martijn , GUIJT, Kees-Jan , ZOU, Zhengzheng , TANG, Zhongmei , QIAN, Zhen , GE, Jing , ZHANG, Zhenghong
CPC分类号： C12P19/02 , C12P19/14 , C12Y302/01001 , C12Y302/01003
摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.
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公开(公告)号：WO2020010101A2
公开(公告)日：2020-01-09
申请号：PCT/US2019/040331
申请日：2019-07-02
申请人： DANISCO US INC
发明人： TANG, Zhongmei , WU, Qihui , XI, Xingxiang , ZHANG, Zhenghong
摘要： Described are methods of saccharifying starch-containing materials using a glucoamylase, the methods of producing fermentation products and the fermentation products produced by the method thereof.
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3.发明申请
公开(公告)号：WO2019209559A1
公开(公告)日：2019-10-31
申请号：PCT/US2019/027426
申请日：2019-04-15
申请人： DANISCO US INC
摘要： Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.
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公开(公告)号：WO2019047199A1
公开(公告)日：2019-03-14
申请号：PCT/CN2017/101177
申请日：2017-09-11
申请人： DANISCO US INC. , HAO, Helong , TANG, Zhongmei , XIE, Zhiyong , ZHANG, Zhenghong
发明人： HAO, Helong , TANG, Zhongmei , XIE, Zhiyong , ZHANG, Zhenghong
摘要： Described are methods of saccharifying starch-containing materials using a glucoamylase, as well as the methods of producing fermentation products and the fermentation products produced by the method thereof.
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5.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION 审中-公开从ASPERGILLUS FUMIGATUS和ISOAMYLASE使用ALPHA-AMYLASE进行鉴定的方法
公开(公告)号：WO2014093125A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/073317
申请日：2013-12-05
申请人： DANISCO US INC.
发明人： HUA, Ling , SCHEFFERS, Martijn , VAN BRUSSEL, Marco , VROEMEN, Casper , ZHANG, Bo , ZHANG, Zhenghong
CPC分类号： C12P19/14 , A21D8/042 , A23L7/107 , A23V2002/00 , C11D3/386 , C12C5/004 , C12C11/003 , C12N9/242 , C12N9/246 , C12P7/06 , C12P19/02 , C12Y302/01001 , C12Y302/01068 , D06L1/06 , Y02E50/17
摘要： A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30 - 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI + DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
摘要翻译： 真菌α-淀粉酶由烟曲霉（AfAmyl）提供。 AfAmyl具有3.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川崎曲霉α-淀粉酶催化的糖化产物相比，AfAmyl还催化淀粉底物对显着富集DP2和（DPI + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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公开(公告)号：WO2023034486A2
公开(公告)日：2023-03-09
申请号：PCT/US2022/042317
申请日：2022-09-01
申请人： DANISCO US INC.
发明人： LASSILA, Jonathan , ZHANG, Zhenghong , ZHONG, Kun
摘要： Disclosed herein are compositions and methods for preventing, reducing, or removing biofilms.
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公开(公告)号：WO2020010101A3
公开(公告)日：2020-01-09
申请号：PCT/US2019/040331
申请日：2019-07-02
申请人： DANISCO US INC
发明人： TANG, Zhongmei , WU, Qihui , XI, Xingxiang , ZHANG, Zhenghong
摘要： Described are methods of saccharifying starch-containing materials using a glucoamylase, the methods of producing fermentation products and the fermentation products produced by the method thereof.
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8.发明申请METHOD OF USING ALPHA-AMYLASE FROM TALAROMYCES EMERSONII FOR SACCHARIFICATION 审中-公开使用来自TALAROMYCES EMERSONII的ALPHA-淀粉酶进行鉴定的方法
公开(公告)号：WO2014058572A1
公开(公告)日：2014-04-17
申请号：PCT/US2013/060068
申请日：2013-09-17
申请人： DANISCO US INC.
发明人： HUA, Ling , TANG, Zhongmei , VROEMEN, Casper , ZHANG, Bo , ZHANG, Zhenghong
CPC分类号： C12N9/242 , A21D8/042 , A23L29/35 , C11D3/386 , C12C5/004 , C12C7/04 , C12C7/14 , C12C11/003 , C12C11/07 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06L1/12 , D06L1/14 , D06M16/003 , Y02E50/16 , Y02E50/17
摘要： A fungal alpha amylase is provided from Talaromyces emersonii (TeAmy1), along with variants of the same. TeAmy1 has an optimal pH of 3.5 and is operable over a temperature range of at least 30 75ºC, allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. TeAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii . This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
摘要翻译： 真菌α淀粉酶从艾美（Talaromyces emersonii）（TeAmy1）提供，以及相同的变体。 TeAmy1具有3.5的最佳pH，并且在至少30±75℃的温度范围内可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇方法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比，TeAmy1还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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公开(公告)号：WO2023064905A1
公开(公告)日：2023-04-20
申请号：PCT/US2022/078121
申请日：2022-10-14
申请人： DANISCO US INC. , SCHEFFERS, Martijn , IANCU, Svetlana, Laura , VAN BRUSSEL-ZWIJNEN, Marco , KRUITHOF, Paulien , SORG, Robin , TANG, Zhongmei , ZHANG, Zhenghong , SHANG, Yuepeng , KOZAK, Barbara , NIKOLAEV, Igor , LASSILA, Jonathan
发明人： SCHEFFERS, Martijn , IANCU, Svetlana, Laura , VAN BRUSSEL-ZWIJNEN, Marco , KRUITHOF, Paulien , SORG, Robin , TANG, Zhongmei , ZHANG, Zhenghong , SHANG, Yuepeng , KOZAK, Barbara , NIKOLAEV, Igor , LASSILA, Jonathan
摘要： Described herein, inter cilia, are glucoamylase variants and methods of using the same for saccharifying a starch substrate. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage using said as well as a. method of producing a fermented beverage using said glucoamylase variants.
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公开(公告)号：WO2021212095A1
公开(公告)日：2021-10-21
申请号：PCT/US2021/027894
申请日：2021-04-19
发明人： TANG, Zhongmei , ZHANG, Zhenghong , GE, Jing , BABE, Lilia , XI, Xingxiang , HAO, Helong , HUANG, Chao
摘要： Described are a recombinant host cell, a composition comprising a glucoamylase and methods of saccharifying the starch substrate using the glucoamylase. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage. The glucoamylase or 1,4-alpha-D- glucan glucohydrolase (EC 3.2.1.3) is preferably from the Mucorales- clade, especially having high sequence identity to the glucoamylase from Saksenaea vasiformis.
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