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    RANDOM ACCESS STIMULATED EMISSION DEPLETION (STED) MICROSCOPY
    1.
    发明申请
    RANDOM ACCESS STIMULATED EMISSION DEPLETION (STED) MICROSCOPY 审中-公开
    随机访问刺激排泄（STED）显微镜

    公开(公告)号：WO2014147590A1

    公开(公告)日：2014-09-25

    申请号：PCT/IB2014/060024

    申请日：2014-03-21

    Applicant: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA ,  BAYLOR COLLEGE OF MEDICINE

    Inventor： BIANCHINI, Paolo ,  SAGGAU, Peter ,  DIASPRO, Alberto

    IPC: G02B21/00 ,  G02F1/33 ,  G01N21/64 ,  G02B21/16 ,  G02B27/58

    CPC classification number: G02F1/33 ,  G01N21/6456 ,  G02B21/002 ,  G02B21/0036 ,  G02B21/0076 ,  G02B21/0092 ,  G02B21/16 ,  G02B27/09 ,  G02B27/58

    Abstract: Optical scanning system (80), comprising an optical system (3) for guiding a first and a second light beam (110, 110b), and deflector devices for deflecting first and second light beams in a directionally variable manner. The deflector devices comprise at least one acousto-optic deflector (100.1, 100.2; 100.3, 100.4), and the optical system is arranged in such a way that the first and second light beams are counter-propagating through the acousto-optic deflector, which is controllable for deflecting the first and second light beams simultaneously or in pulse sequence. STED microscopy apparatus comprising an optical scanning system based on acousto-optic deflectors.

    Abstract translation: 光学扫描系统（80），包括用于引导第一和第二光束（110,110b）的光学系统（3）和用于以方向可变方式偏转第一和第二光束的偏转器装置。 偏转器装置包括至少一个声光偏转器（100.1,100.2; 100.3,100.4），并且光学系统被布置成使得第一和第二光束通过声光偏转器相反传播， 是可控制的，以同时或以脉冲顺序偏转第一和第二光束。 包括基于声光偏转器的光学扫描系统的STED显微镜装置。

    MICRO-INTERFEROMETER FOR N-TH HARMONIC INTERFERENCE MICROSCOPY
    2.
    发明申请
    MICRO-INTERFEROMETER FOR N-TH HARMONIC INTERFERENCE MICROSCOPY 审中-公开
    用于N-TH谐波干涉显微镜的微型干涉仪

    公开(公告)号：WO2014041497A1

    公开(公告)日：2014-03-20

    申请号：PCT/IB2013/058483

    申请日：2013-09-12

    Applicant: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

    Inventor： BRANDI, Fernando ,  DIASPRO, Alberto

    IPC: G01B9/02 ,  G01N21/45

    CPC classification number: G01B9/02007 ,  G01B9/02024 ,  G01N21/45 ,  G02B21/14

    Abstract: Device for optically measuring a medium, comprising a light source (1) which provides a fundamental beam (FB) with a first wavelength; a first harmonic generator (3) which generates from the fundamental beam (FB) a first harmonic beam (HB1) with a second wavelength; an optical system (7, 9) which couples light of the fundamental beam (FB) and the first harmonic beam (HB1) along a single, common light path; a second harmonic genera- tor (11) positioned after the target area (5); and a detector (15) which detects light from the medium to measure a change in phase of the light interacting with the medium. The optical system comprises an achromatic focusing system (7) and an achromatic collimating system (9) positioned before and after the target area (5) so as to have respective focal points substantially coincident at the target area (5). The first and second harmonic generators are positioned before the achromatic focusing system (7) and after the achromatic collimating system (9), respectively.

    Abstract translation: 用于光学测量介质的装置，包括提供具有第一波长的基波束（FB）的光源（1） 从基波束（FB）产生具有第二波长的一次谐波波束（HB1）的一次谐波发生器（3） 光学系统（7,9），其沿着单个公共光路耦合所述基波束（FB）和所述第一谐波波束（HB1）的光; 定位在目标区域（5）之后的二次谐波发生器（11）; 以及检测器（15），其检测来自介质的光以测量与介质相互作用的光的相位变化。 光学系统包括消色差聚焦系统（7）和定位在目标区域（5）之前和之后的消色差准直系统（9），以使各个焦点基本上与目标区域（5）重合。 第一和第二谐波发生器分别位于消色差聚焦系统（7）之前和消色差准直系统（9）之后。

    METHOD OF LIGHTNENING AT LEAST ONE BIOLOGICAL SAMPLE, THREE-DIMENSIONAL HIGH RESOLUTION DEPLETION MICROSCOPY METHOD AND CORRESPONDING MICROSCOPE
    3.
    发明申请
    METHOD OF LIGHTNENING AT LEAST ONE BIOLOGICAL SAMPLE, THREE-DIMENSIONAL HIGH RESOLUTION DEPLETION MICROSCOPY METHOD AND CORRESPONDING MICROSCOPE 审中-公开

    公开(公告)号：WO2019106614A1

    公开(公告)日：2019-06-06

    申请号：PCT/IB2018/059492

    申请日：2018-11-30

    Applicant: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

    Inventor： BIANCHINI, Paolo ,  DEGUCHI, Takahiro ,  DIASPRO, Alberto

    IPC: G02B21/00 ,  G01N21/64 ,  G02B21/16 ,  G02B27/58

    CPC classification number: G02B27/58 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16

    Abstract: Lightening method of at least one biological sample (S), in which said at least one biological sample includes at least one or more fluorophores, at the focal point (F) of at least one objective lens (L) having a main optical axis (z-z), the method comprising the following operational steps:- lightening (step 10) said at least one biological sample (S) with at least one excitation beam (EB), which propagates between said at least one objective lens (L) and said at least one biological sample (S) along at least one first propagation axis (a-a);- lightening (step 20) said at least one biological sample (S) with at least two depletion beams (DB, DB'), which propagate between said at least one objective lens (L) and said at least one biological sample (S) along the respective second propagation axes (b-b, b'-b'), said depletion beams being donut-shaped, each one in a plane orthogonal to the respective second propagation axis (b-b, b'-b'); whereby said at least one first propagation axis (a-a) and said at least second propagation axes (b-b, b'-b') are angularly inclined with each other, and said at least one first propagation axis (a-a) and said second propagation axes (b-b, b'-b') intersect on said at least one biological sample (s) only at the focal point (F) of said at least one objective lens (L), so that an effective fluorescence volume (FV) is generated in said at least one biological sample (S) which is limited both orthogonally and axially with respect to said main optical axis (z-z).

    MICROSCOPY METHOD AND APPARATUS FOR OPTICAL TRACKING OF EMITTER OBJECTS
    4.
    发明申请
    MICROSCOPY METHOD AND APPARATUS FOR OPTICAL TRACKING OF EMITTER OBJECTS 审中-公开

    公开(公告)号：WO2018134730A1

    公开(公告)日：2018-07-26

    申请号：PCT/IB2018/050257

    申请日：2018-01-16

    Applicant: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

    Inventor： DUOCASTELLA, Marti ,  SANCATALDO, Giuseppe ,  DIASPRO, Alberto

    IPC: G02B27/00 ,  G02B21/02 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36

    Abstract: Microscopy method and apparatus for determining the positions of one or more emitter objects in a three-dimensional space that comprises focusing scattered light or fluorescent light emitted by an emitter object, separating the focused beam in a first and a second optical beam, directing the first and the second optical beam through a varifocal lens having an optical axis in such a way that the first optical beam impinges on the lens along the optical axis and the second beam impinges decentralized with respect to the optical axis of the varifocal lens, simultaneously capturing a first image created by the first optical beam and a second image created by the second optical beam, and determining the relative displacement of the position of the object in the first and in the second image, wherein the relative displacement contains the information of the axial position of the object along a perpendicular direction to the image plane.

    METHOD FOR THE PREPARATION OF A SUBSTRATE FOR A PLASMONIC DEVICE
    5.
    发明申请
    METHOD FOR THE PREPARATION OF A SUBSTRATE FOR A PLASMONIC DEVICE 审中-公开
    用于制备等离子体装置的基板的方法

    公开(公告)号：WO2015015423A1

    公开(公告)日：2015-02-05

    申请号：PCT/IB2014/063524

    申请日：2014-07-29

    Applicant: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

    Inventor： INTARTAGLIA, Romuald ,  BEKE, Szabolcs ,  DAS, Gobind ,  DIASPRO, Alberto ,  BRANDI, Fernando

    IPC: G01N21/65 ,  B82Y40/00

    CPC classification number: G01N21/658 ,  B82Y30/00 ,  B82Y40/00

    Abstract: The present invention concerns a method for the preparation of a substrate for a plasmonic device comprising the steps of: - providing a substrate (1) made of a photosensitive glass having a first surface (2); - treating the first surface (2) with a light beam so as to obtain a plurality of substantially conical microstructures (6), - depositing on the plurality of microstructures (6) a layer of metal nanoparticles (7) produced by laser irradiation of a metal sheet in water or in an aqueous solution in which the treatment step with a light beam is performed by means of the following steps: - positioning of a masking element (3) on the first surface (2) so as to define in at least one direction a first plurality of masked portions (4) and a second plurality of unmasked portions (5); - irradiation with UV radiation of the plurality of unmasked portions ( 5 ); - removal of the masking element (3); - heating of the substrate (1) and - treatment of the first surface (2) with a strong acid.

    Abstract translation: 本发明涉及一种制备等离子体元件的衬底的方法，包括以下步骤： - 提供由具有第一表面（2）的感光玻璃制成的衬底（1）; - 用光束处理所述第一表面（2）以获得多个基本上圆锥形的微结构（6）; - 在所述多个微结构（6）上沉积金属纳米颗粒（7），所述金属纳米粒子（7）由激光照射 在水中或水溶液中的金属片，其中通过以下步骤进行具有光束的处理步骤： - 将掩模元件（3）定位在第一表面（2）上，以便至少限定 一个方向是第一多个掩模部分（4）和第二多个未掩模部分（5）; - 多个未掩模部分（5）的UV辐射照射; - 去除掩模元件（3）; - 加热基材（1）和 - 用强酸处理第一表面（2）。

    STIMULATED EMISSION-DEPLETION (STED) MICROSCOPY BASED ON TIME GATING OF EXCITATION BEAM AND SYNCHRONOUS DETECTION OF FLUORESCENCE EMISSION
    7.
    发明申请
    STIMULATED EMISSION-DEPLETION (STED) MICROSCOPY BASED ON TIME GATING OF EXCITATION BEAM AND SYNCHRONOUS DETECTION OF FLUORESCENCE EMISSION 审中-公开
    基于激发光束的时间增益和荧光发射同步检测的刺激排放（STED）显微镜

    公开(公告)号：WO2015022635A1

    公开(公告)日：2015-02-19

    申请号：PCT/IB2014/063872

    申请日：2014-08-12

    Applicant: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

    Inventor： VICIDOMINI, Giuseppe ,  HARKE, Benjamin ,  DIASPRO, Alberto

    IPC: G01N21/64

    CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N2021/6439

    Abstract: Method of optical microscopy by scanning a sample containing an excitable species, the method comprising: - directing a first and a second light beam onto respective, partially overlapped areas of the sample, wherein the first light beam is provided for exciting members of the excitable species, and the second light beam is provided for reducing the number of excited members; - detecting an optical signal coming from the sample, comprising a main component and a spurious component, during consecutive first and second time gates, the first time gate being provided for detecting the optical signal for a time interval during which the main component and the spurious component are both present, and the second time gate being provided for detecting the optical signal for a time interval during which the main component tends to or is zero; - processing the detected optical signal to separate its main component. The first light beam is operating during the first time gate and is interrupted during the second time gate.

    Abstract translation: 通过扫描含有可兴奋物质的样品的光学显微镜方法，所述方法包括：将第一和第二光束引导到样品的相应部分重叠的区域上，其中第一光束被提供用于激发可兴奋物质的成员 并且第二光束被设置用于减少激励构件的数量; - 在连续的第一和第二时间门期间，检测来自采样的光信号，包括主分量和杂散分量，第一时间门被提供用于在主分量和杂散的时间间隔内检测光信号 分量都存在，并且第二时间门被提供用于在主分量倾向于或为零的时间间隔内检测光信号; - 处理检测到的光信号以分离其主要部件。 第一光束在第一时间栅极期间操作并且在第二时间栅极期间中断。

    TIME-RESOLVED IMAGING METHOD WITH HIGH SPATIAL RESOLUTION
    10.
    发明申请
    TIME-RESOLVED IMAGING METHOD WITH HIGH SPATIAL RESOLUTION 审中-公开

    公开(公告)号：WO2019145889A1

    公开(公告)日：2019-08-01

    申请号：PCT/IB2019/050595

    申请日：2019-01-24

    Applicant: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA ,  POLITECNICO DI MILANO ,  UNIVERSITA' DEGLI STUDI DI GENOVA

    Inventor： VICIDOMINI, Giuseppe ,  CASTELLO, Marco ,  TORTAROLO, Giorgio ,  TOSI, Alberto ,  BUTTAFAVA, Mauro ,  VILLA, Federica ,  BIANCHINI, Paolo ,  DIASPRO, Alberto ,  SHEPPARD, Colin J.R.

    IPC: G02B21/00 ,  G02B27/58

    Abstract: Method for operating a point laser- scanning microscope, comprising scanning a sample with a focused illumination laser beam (EB); recording, by means of an array of detecting elements (23), a plurality of images of the sample over a scan by the laser beam, wherein the detecting elements (23) are configurable to an intensity mode, in which the recorded images are intensity images g i,j (n) related to photons collected during an entire dwell time of the illumination beam on the individual position n, or in a time-resolved mode, in which the recorded images are time -resolved images g t ij (n, t ), in which the collected photons are discriminated based on their times of arrival to the individual detecting elements (23); calculating a fingerprint image a by summing the plurality of intensity images g i,j (n) over all positions n of the illumination beam (EB) on the sample, the fingerprint image depending simultaneously on an illumination point-spread function, hereinafter illumination PSF, h exc , and a detection point-spread function, hereinafter detection PSF, h det , estimating shift matrices s x and s y from the intensity images g i,j (n), reconstructing at least one of: i) a time-resolved object function f t , and ii) an intensity object function f, and, visualizing at least one of a high-resolution time-resolved image f t~ and a high- resolution intensity image f ~ , based on said time -resolved object function and intensity object function.

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