公开(公告)号：EP1948822A4

公开(公告)日：2009-10-28

申请号：EP06790343

申请日：2006-10-06

申请人： JOHNSON & JOHNSON RES PTY LTD

发明人： MOKANY ELISA ,  BIRKETT DONALD JOHN ,  TODD ALISON VELYIAN ,  DOAN TRAM BICH

IPC分类号： C12Q1/68 ,  C12N9/00

CPC分类号： C07H21/04 ,  C12N15/111 ,  C12N2310/12 ,  C12N2320/10 ,  C12N2330/30 ,  C12Q1/6811 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q1/6823 ,  C12Q2537/125 ,  C12Q2525/161 ,  C12Q2521/337 ,  C12Q2525/205

公开(公告)号：EP1857555B1

公开(公告)日：2008-12-31

申请号：EP07013576.9

申请日：2004-10-01

申请人： STERNA BIOLOGICALS GmbH & Co. KG

发明人： Sel, Serdar, Dr. ,  Renz, Harald, Prof. Dr.

IPC分类号： C12N15/63 ,  A61K48/00 ,  C12N15/10

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C12N15/1136 ,  C12N2310/12 ,  C12N2310/14 ,  C12Q1/6809 ,  C12Q1/6883 ,  C12Q2521/337

公开(公告)号：EP1062367B1

公开(公告)日：2008-11-12

申请号：EP99914694.7

申请日：1999-02-22

申请人： JOHNSON & JOHNSON RESEARCH PTY LIMITED

发明人： TODD, Alison ,  FUERY, Caroline, J. ,  CAIRNS, Murray

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6867 ,  C12Q2545/114 ,  C12Q2537/143 ,  C12Q2521/337 ,  C12Q2525/149 ,  C12Q2525/161 ,  C12Q2563/125 ,  C12Q2531/125 ,  C12Q2531/113

摘要： This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.

公开(公告)号：EP1504018B1

公开(公告)日：2008-08-13

申请号：EP03731042.2

申请日：2003-04-24

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： LU, Yi ,  LIU, Juewen

IPC分类号： C12Q1/68 ,  C07H21/04 ,  G01N33/58 ,  C12Q1/34

CPC分类号： C12Q1/68 ,  C12Q1/34 ,  C12Q1/6816 ,  G01N33/585 ,  Y10T436/143333 ,  C12Q2563/137 ,  C12Q2521/337

摘要： Disclosed are compositions and methods for the sensitive and selective detection of ions using nucleic acid enzymes and DNA modified microparticles.

公开(公告)号：EP1882746A2

公开(公告)日：2008-01-30

申请号：EP07111614.9

申请日：2007-07-12

申请人： Epigenomics AG

发明人： Berlin, Kurt ,  Distler, Jürgen ,  Tetzner, Reimo

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q1/6851 ,  C12Q2521/337 ,  C12Q2523/125 ,  C12Q2525/191 ,  C12Q2531/125

摘要： The present invention relates to a method for detecting the presence of one or more methylation patterns. It comprises the conversion of nucleic acids and a catalytic nucleic acid activity. Here, the conversion of the nucleic acid is characterized such that the 5-methylcytosine remains unchanged while the unmethylated cytosine is converted into uracil or another base, which can be distinguished from cytosine in its base-pairing behavior.

摘要翻译： 本发明涉及用于检测一种或多种甲基化模式的存在的方法。 它包括核酸的转化和催化核酸活性。 在此，核酸转化的特征在于，5-甲基胞嘧啶保持不变，而未甲基化的胞嘧啶转变为尿嘧啶或另一种碱基，其可以与胞嘧啶的碱基配对行为区分开来。

公开(公告)号：EP1717313A4

公开(公告)日：2007-11-14

申请号：EP04787923

申请日：2004-09-21

申请人： UNIV KYOTO

发明人： AOYAMA YASUHIRO ,  SANDO SHINSUKE ,  SASAKI TOSHINORI ,  NARITA ATSUSHI

IPC分类号： C12N15/11 ,  C12M1/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566

CPC分类号： C12Q1/683 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q2521/337

摘要： A nucleic acid probe for highly sensitively, highly accurately and quickly detecting a target nucleic acid in a trace amount which is designed so that, after hybridization with the target nucleic acid, the nucleic acid probe undergoes a structural change, thus shows a lowered binding force in the hybridization and, in its turn, is detached from the target nucleic acid. This nucleic acid probe comprises a cleavage part which is hybridizable with the target nucleic acid and, upon the hybridization with the target nucleic acid, cleaved to thereby give a first probe fragment and a second probe fragment at least one of which can be detached from the target nucleic acid, and a signal generation part capable of generating a signal. This nucleic acid probe has a cleavage part at which it is cleaved upon the hybridization with the target nucleic acid and, until the hybridization with the target nucleic acid, parts of the nucleic acid probe are hybridized with each other in its molecule to form a locked structure.

公开(公告)号：EP1651776A2

公开(公告)日：2006-05-03

申请号：EP04763506.5

申请日：2004-07-26

申请人： QIAGEN GmbH

发明人： WILLE, Tanja ,  KORFHAGE, Christian ,  LADER, Eric

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6848 ,  C12Q1/6809 ,  C12Q2521/107 ,  C12Q2521/337 ,  C12Q2525/107 ,  C12Q2527/137 ,  C12Q2527/127

摘要： The invention relates to a method for the reverse transcription and/or amplification of a product from a reverse transcription of a pool of nucleic acids of a certain type, whereby said pool of nucleic acids is derived from a complex biological sample or an enzymatic reaction.

公开(公告)号：EP1601790A1

公开(公告)日：2005-12-07

申请号：EP04707454.7

申请日：2004-02-02

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： LU, Yi ,  LIU, Juewen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/25 ,  C12Q1/68 ,  C12Q2563/137 ,  C12Q2525/205 ,  C12Q2521/337

摘要： Sensors comprising aptazymes capable of detecting the presence and concentration of effectors, as well as methods of using such sensors, are disclosed.

公开(公告)号：EP0759992B1

公开(公告)日：2004-10-27

申请号：EP95920437.1

申请日：1995-05-15

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  THE BOARD OF REGENTS FOR NORTHERN ILLINOIS UNIVERSITY

发明人： HAMPEL, Arnold ,  DIPAOLO, Joseph ,  SIWKOWSKI, Andrew

IPC分类号： C12N15/52 ,  C12N9/00 ,  A61K31/70 ,  C12Q1/68

CPC分类号： C12N15/1131 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2799/027 ,  C12Q1/683 ,  C12Q1/708 ,  C12Q2521/337

公开(公告)号：EP1354062A2

公开(公告)日：2003-10-22

申请号：EP01971029.2

申请日：2001-09-13

申请人： Archemix Corporation

发明人： STANTON, Marty ,  EPSTEIN, David ,  HAMAGUCHI, Nobuko

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  B01J2219/00385 ,  B01J2219/00479 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00662 ,  B01J2219/00691 ,  B01J2219/00722 ,  B82Y30/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/485 ,  C12Q1/6825 ,  C40B40/06 ,  C40B60/14 ,  C12Q2565/101 ,  C12Q2525/179 ,  C12Q2521/337 ,  C12Q2521/501

摘要： Methods for engineering a target activated biosensor are provided. Biosensors comprise a plurality of nucleic acid sensor molecules labeled with a first signaling moiety and a second signaling moiety. The nucleic acid sensor molecules recognizes target molecules which do not naturally bind to DNA. Binding of a target molecule to the sensor molecules triggers a change in the proximity of the signaling moieties which leads to a change in the optical properties of the nucleic acid sensor molecules on the biosensor. Reagents and systems for performing the method are also provided. The method is useful in diagnostic applications and drug optimization.