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    NOVEL CELLULAR GLYCAN COMPOSITIONS
    13.
    发明公开
    NOVEL CELLULAR GLYCAN COMPOSITIONS 审中-公开
    新型细胞甘氨酸组合物

    公开(公告)号：EP2047257A1

    公开(公告)日：2009-04-15

    申请号：EP07788780.0

    申请日：2007-06-29

    申请人： Suomen Punainen Risti, Veripalvelu ,  Glykos Finland Oy

    发明人： LAINE, Jarmo ,  SATOMAA, Tero ,  NATUNEN, Jari ,  HEISKANEN, Annamari ,  SAARINEN, Juhani ,  JAATINEN, Taina ,  MIKKOLA, Milla ,  NATUNEN, Suvi

    IPC分类号： G01N33/50 ,  C12N5/06

    CPC分类号： G01N33/6848 ,  C08B37/0006 ,  C08B37/006 ,  C12N5/0606 ,  G01N33/56966 ,  G01N2400/10

    摘要： The invention describes novel compositions of glycans, glycomes, from human embryonic stem cells, and especially novel subcompositions of the glycomes with specific monosaccharide compositions and glycan structures. The invention is further directed to methods for modifying the glycomes and analysis of the glycomes and the modified glycomes. Furthermore, the invention is directed to stem cells carrying the modified glycomes on their surfaces. The glycomes are preferably analysed by profiling methods able to detect reproducibly and quantitatively numerous individual glycan structures at the same time. The most preferred type of the profile is a mass spectrometric profile. The invention specifically revealed novel target structures and is especially directed to the development of reagents recognizing the structures.

    摘要翻译： 本发明描述了来自人胚胎干细胞的聚糖，糖类的新型组合物，尤其是具有特定单糖组成和聚糖结构的糖类的新型亚组合物。 本发明还涉及修饰糖类和糖类和修饰糖类分析的方法。 此外，本发明涉及在其表面上携带修饰的糖基的干细胞。 优选通过分析方法分析糖类，所述方法能够同时检测可重复和定量的多种单独的聚糖结构。 该配置文件的最优选类型是质谱配置文件。 本发明具体揭示了新颖的靶结构并且特别针对开发识别结构的试剂。

    PAYLOAD-POLYMER-PROTEIN CONJUGATES
    17.
    发明公开
    PAYLOAD-POLYMER-PROTEIN CONJUGATES 审中-公开
    有效载荷聚合物蛋白KONJUGATE

    公开(公告)号：EP3166638A1

    公开(公告)日：2017-05-17

    申请号：EP15733782.5

    申请日：2015-06-12

    申请人： Glykos Finland Oy

    发明人： HELIN, Jari ,  SAARINEN, Juhani ,  EKHOLM, Filip S. ,  LEPPÄNEN, Anne

    IPC分类号： A61K41/00 ,  A61K47/50 ,  A61P35/00

    CPC分类号： A61K47/48692 ,  A61K41/0095 ,  A61K47/48384 ,  A61K47/6803 ,  A61K47/6849 ,  A61K47/6883

    摘要： The present invention relates to a conjugate represented by the formula [D-L- Y-(CH2)
    n -O]
    m -P-T wherein T is a protein; P is a polymer selected from the group consisting of dextran, mannan, pullulan, hyaluronic acid, hydroxyethyl starch, chondroitin sulphate, heparin, heparin sulphate, polyalkylene glycol, Ficoll, polyvinyl alcohol, amylose, amylopectin, chitosan, cyclodextrin, pectin and carrageenan, or a derivative thereof; m is at least 1; n is in the range of 1 to 10; each Y is independently selected from the group consisting of S, NH and 1,2,3-triazolyl, wherein 1,2,3- triazolyl is optionally substituted; each L is independently absent or comprises a linker group covalently joining D and Y; and each D is a payload molecule.

    摘要翻译： 本发明涉及由式[D-L-Y-（CH2）n-O] m-P-T表示的结合物，其中T是蛋白质; P是选自葡聚糖，甘露聚糖，支链淀粉，透明质酸，羟乙基淀粉，硫酸软骨素，肝素，硫酸肝素，聚亚烷基二醇，Ficoll，聚乙烯醇，直链淀粉，支链淀粉，壳聚糖，环糊精，果胶和角叉菜胶的聚合物， 或其衍生物; m至少为1; n在1〜10的范围内; 每个Y独立地选自S，NH和1,2,3-三唑基，其中1,2,3-三唑基任选被取代; 每个L独立地不存在或包含共价连接D和Y的连接基团; 并且每个D是有效载荷分子。

    PRODUCTION OF GLYCOPROTEINS HAVING INCREASED N-GLYCOSYLATION SITE OCCUPANCY
    18.
    发明公开
    PRODUCTION OF GLYCOPROTEINS HAVING INCREASED N-GLYCOSYLATION SITE OCCUPANCY 审中-公开
    枸杞子

    公开(公告)号：EP3019621A1

    公开(公告)日：2016-05-18

    申请号：EP14738496.0

    申请日：2014-07-10

    申请人： Novartis AG ,  Glykos Finland Oy

    发明人： NATUNEN, Jari ,  LANDOWSKI, Christopher ,  SALOHEIMO, Markku ,  OSTERMEIER, Christian ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon

    IPC分类号： C12P21/00 ,  C12N9/10

    CPC分类号： C12P21/005 ,  C07K16/00 ,  C12N9/1051 ,  C12N9/1081 ,  C12N15/80 ,  C12Y204/01

    摘要： The present disclosure relates to compositions and methods useful for the production of heterologous proteins with increased N-glycosylation site occupancy in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutation that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s), ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase, and iii. a polynucleotide encoding a heterologous glycoprotein, wherein said catalytic subunit of oligosaccharyl transferase is selected from Leishmania oligosaccharyl transferase catalytic subunits.

    摘要翻译： 本公开内容涉及用于生产丝状真菌细胞如木霉属细胞中具有增加的N-糖基化位点占据的异源蛋白质的组合物和方法。 更具体地，本发明提供丝状真菌细胞，其包含i。 一种或多种与不具有所述突变的亲本丝状真菌细胞相比减少或消除一种或多种内源蛋白酶活性的突变，ii。 编码寡糖基转移酶的异源催化亚基的多核苷酸，和iii。 编码异源糖蛋白的多核苷酸，其中所述寡糖基转移酶的催化亚单位选自利什曼原虫寡糖基转移酶催化亚基。

    O-MANNOSYLTRANSFERASE DEFICIENT FILAMENTOUS FUNGAL CELLS AND METHODS OF USE THEREOF
    19.
    发明公开
    O-MANNOSYLTRANSFERASE DEFICIENT FILAMENTOUS FUNGAL CELLS AND METHODS OF USE THEREOF 审中-公开
    O-MANNOSYLTRANSFERASEDEFIZIENTEFILAMENTÖSEPILZZELLEN UND VERFAHREN ZUR VERWENDUNG DAVON

    公开(公告)号：EP3016970A1

    公开(公告)日：2016-05-11

    申请号：EP14734515.1

    申请日：2014-07-03

    申请人： Novartis AG ,  Glykos Finland Oy

    发明人： NATUNEN, Jari ,  HILTUNEN, Jukka ,  HUUSKONEN, Anne ,  SALOHEIMO, Markku ,  OSTERMEIER, Christian ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon

    IPC分类号： C07K14/37 ,  C07K16/00

    CPC分类号： C12N9/1051 ,  C07K14/37 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/41 ,  C12Y204/01109

    摘要： The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a PMT-deficient filamentous fungal cell comprising a) at least a first mutation that reduces an endogenous protease activity compared to a parental filamentous fungal cell which does not have said first mutation, and, b) at least a second mutation in a PMT gene that reduces endogenous O-mannosyltransferase activity compared to a parental filamentous fungal cell which does not have said second mutation, wherein said filamentous fungal cell is selected from the group consisting of Trichoderma, Neurospora, Myceliophthora or Chrysosporium cell.

    摘要翻译： 本公开涉及用于生产丝状真菌细胞如木霉属细胞中具有降低的O-甘露糖基化的异源蛋白质的组合物和方法。 更具体地，本发明提供了一种缺乏PMT的丝状真菌细胞，其包含a）至少一种与不具有所述第一突变的亲本丝状真菌细胞相比降低内源蛋白酶活性的第一突变，和b）至少第二突变 与不具有第二突变的亲本丝状真菌细胞相比，降低内源性O-甘露糖基转移酶活性的PMT基因中的突变，其中所述丝状真菌细胞选自木霉属，孢子孢子虫，丝霉菌属或孢子孢子菌属细菌。