公开(公告)号：EP2483680A4

公开(公告)日：2014-01-01

申请号：EP10820963

申请日：2010-05-13

申请人： QUANTAPORE INC

发明人： HUBER MARTIN

IPC分类号： G01N33/00 ,  B82B3/00 ,  C12Q1/68 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N21/6428 ,  G01N33/48721 ,  G01N2021/6432 ,  C12Q2563/155 ,  C12Q2563/119 ,  C12Q2563/107 ,  C12Q2565/531 ,  C12Q2565/101 ,  C12Q2561/113

公开(公告)号：EP2483680A1

公开(公告)日：2012-08-08

申请号：EP10820963.6

申请日：2010-05-13

申请人： Quantapore Inc.

发明人： HUBER, Martin

IPC分类号： G01N33/00 ,  B82B3/00

CPC分类号： C12Q1/6869 ,  G01N21/6428 ,  G01N33/48721 ,  G01N2021/6432 ,  C12Q2563/155 ,  C12Q2563/119 ,  C12Q2563/107 ,  C12Q2565/531 ,  C12Q2565/101 ,  C12Q2561/113

摘要： Methods and systems for sequencing a biological molecule or polymer, e.g., a nucleic acid, are provided. One or more donor labels, which are attached to a pore or nanopore, may be illuminated or otherwise excited. A polymer having a monomer labeled with one or more acceptor labels, may be translocated through the pore. Either before, after or while the labeled monomer of the polymer passes through, exits or enters the pore, energy may be transferred from the excited donor label to the acceptor label of the monomer. As a result of the energy transfer, the acceptor label emits energy, and the emitted energy is detected in order to identify the labeled monomer of the translocated polymer and to thereby sequence the polymer.

公开(公告)号：EP1394270B1

公开(公告)日：2008-03-12

申请号：EP03018640.7

申请日：2003-08-20

申请人： BioArray Solutions Ltd.

发明人： Yang, Jiacheng

IPC分类号： C12Q1/68 ,  G01N33/543 ,  C12Q1/25

CPC分类号： C12Q1/6837 ,  C07K14/003 ,  C07K2319/50 ,  C12N2310/3181 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/485 ,  C12Q1/6827 ,  G01N33/54366 ,  G01N33/6893 ,  G01N33/6896 ,  C12Q2525/107 ,  C12Q2525/161 ,  C12Q2537/137 ,  C12Q2565/518 ,  C12Q2563/119

公开(公告)号：EP1713933A1

公开(公告)日：2006-10-25

申请号：EP05701315.3

申请日：2005-02-02

申请人： INTERNATIONAL UNIVERSITY BREMEN GMBH ,  Universität Basel

发明人： SCHWANEBERG, Ulrich ,  WINTERHALTER, Mathias ,  MEIER, Wolfgang

IPC分类号： C12Q1/68 ,  G01N33/58

CPC分类号： C12N15/1006 ,  C12Q2563/119 ,  C12Q2563/161

摘要： The invention relates to a method which is used to separate substances from liquid media and suitable separation means therefor.

公开(公告)号：EP1664722A2

公开(公告)日：2006-06-07

申请号：EP04784773.6

申请日：2004-09-22

申请人： Bioarray Solutions Ltd

发明人： WANG, Xinwen ,  BANERJEE, Sukanta

IPC分类号： G01N1/00

CPC分类号： C12Q1/6834 ,  C07H21/04 ,  G01N33/54353 ,  C12Q2563/119

摘要： A polyelectrolyte having multiple exposed functional groups, each such group being capable of covalently bonding to a molecule, is immobilized on a surface for the purpose of bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, e.g., an amine functionalized oligonucleotide. The polyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is bound to a functionalized surface using a covalent immobilization strategy, e.g., reaction with the surface of a tosyl-activated microparticle. Following such reaction, exposed reactive functional groups on the protein, such as amine, carboxyl, thiol, hydroxyl groups can further be utilized to covalently couple the oligonucleotide of interest using suitable chemistry.

公开(公告)号：EP1483415A2

公开(公告)日：2004-12-08

申请号：EP03742781.2

申请日：2003-02-19

申请人： UNIVERSITY OF VIRGINIA PATENT FOUNDATION ,  Chroma Therapeutics Limited

发明人： ALLIS, C., David ,  BRUNS, David, E. ,  DRUMMOND, Alan, H.

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： G01N33/6842 ,  C12Q1/6809 ,  G01N33/5308 ,  G01N33/68 ,  G01N33/6875 ,  C12Q2565/113 ,  C12Q2563/119

摘要： The present invention relates to the use of antibodies directed against specific histone amino terminus modifications as diagnostic indicators of disease or congenital defects. In one embodiment, nucleosomes are isolated from a blood or serum sample of a patient using histone specific antibodies and the accompanying DNA is purified and analyzed for diagnostic and screening purposes.

公开(公告)号：EP0912765A1

公开(公告)日：1999-05-06

申请号：EP97930488.0

申请日：1997-07-02

申请人： Roche Diagnostics GmbH

发明人： GIESEN, Ursula ,  WENZIG, Peter ,  ZIEGLER, Günter ,  WEINDEL, Kurt

IPC分类号： G01N33 ,  C12Q1 ,  G01N21

CPC分类号： C12Q1/6816 ,  Y10T436/143333 ,  C12Q2563/113 ,  C12Q2537/143 ,  C12Q2537/101 ,  C12Q2563/119 ,  C12Q2563/107

摘要： The combination of two detection mechanisms in a single apparatus leads to a process that enables the detected analytes to be easily quantified. Appropriate formats and reagent kits are disclosed.

公开(公告)号：EP0562047A4

公开(公告)日：1995-11-02

申请号：EP92904971

申请日：1991-12-06

申请人： AFFYMAX TECH NV

发明人： FODOR STEPHEN P A ,  SOLAS DENNIS W ,  DOWER WILLIAM J

IPC分类号： B01J19/00 ,  C07B61/00 ,  C07H19/10 ,  C07H21/00 ,  C07K1/04 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/14 ,  G03F7/00 ,  G03F7/26 ,  G03F7/38 ,  G11C13/02 ,  C07H15/12 ,  G01N33/48 ,  G01N33/566

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

摘要： The present invention provides methods and appartus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of bioligical samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.

公开(公告)号：EP0635069A1

公开(公告)日：1995-01-25

申请号：EP93912131.0

申请日：1993-04-02

申请人： APPLIED BIOSYSTEMS, INC.

发明人： GROSSMAN, Paul, David ,  FUNG, Steven ,  MENCHEN, Steven, Michael ,  WOO, Sam, Lee ,  WINN-DEEN, Emily, Susan

IPC分类号： C12N15 ,  B01D57 ,  C12Q1 ,  G01N27 ,  G01N30 ,  G01N33

CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  Y10T436/143333 ,  C12Q2565/125 ,  C12Q2525/203 ,  C12Q2525/197 ,  C12Q2563/167 ,  C12Q2535/101 ,  C12Q2565/629 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2531/137 ,  C12Q2563/119 ,  C12Q2549/119 ,  C12Q2525/161 ,  C12Q2521/501 ,  C12Q2563/131 ,  C12Q2525/101 ,  C12Q2531/113

摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In practicing the method, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive elctrophoretic mobility in a sieving matrix, to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.

公开(公告)号：EP0431882A3

公开(公告)日：1992-06-17

申请号：EP90313132.4

申请日：1990-12-04

申请人： ZENECA LIMITED

发明人： Garman, Andrew John ,  Parker, John Rushington

IPC分类号： C12Q1/68 ,  G01N33/532 ,  C07H21/00 ,  C07K17/06 ,  C07K3/08

CPC分类号： G01N33/535 ,  C12Q1/6816 ,  G01N33/532 ,  Y10S435/964 ,  Y10S530/816 ,  C12Q2561/107 ,  C12Q2563/119

摘要： Pre-actived stable protein reagents are provided. The reagents can be made by reaction of the protein and a heterobifunctional linker and have extended stability especially after lyophilisation. The reagents can be used in assay kits to form conjugates with proteins e.g. antibodies or polynucleotides e.g. oligonucleotides probes.