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    COMPOSITIONS AND METHODS FOR PRODUCTION OF AGLYCOSYLATED PLASMINOGEN
    63.
    发明公开
    COMPOSITIONS AND METHODS FOR PRODUCTION OF AGLYCOSYLATED PLASMINOGEN 审中-公开
    组合物及其制造方法AGLYCOSYLIERTEM纤溶酶原

    公开(公告)号：EP2435562A1

    公开(公告)日：2012-04-04

    申请号：EP10730613.6

    申请日：2010-05-26

    申请人： Biolex Therapeutics, Inc.

    发明人： DICKEY, Lynn, F. ,  GASDASKA, John, R. ,  COX, Kevin, M.

    IPC分类号： C12N9/68 ,  A61K38/16 ,  C12N15/82 ,  C12N5/04 ,  C12N9/14

    CPC分类号： A61K38/168 ,  C12N9/6435 ,  C12N15/8257 ,  C12Y304/21007

    摘要： Compositions and methods for producing aglycosylated plasminogen (PLG) polypeptides and fragments and variants thereof are provided. Compositions of the invention include isolated nucleic acid molecules encoding aglycosylated PLG polypeptides in which the asparagine (Asn) residue corresponding to residue Asn-289 of the mature human PLG polypeptide has been substituted with an amino acid residue that does not support
    N -linked glycosylation at that position of the PLG polypeptide, as well as the aglycosylated PLG polypeptides encoded thereby. Expression constructs comprising these PLG-encoding nucleic acid molecules and transgenic plants comprising these expression constructs are also provided. Methods of the invention comprise introducing a PLG-encoding nucleic acid molecule of the invention into a plant of interest and culturing the plant under conditions to produce the aglycosylated PLG polypeptide. The aglycosylated PLG polypeptide allows for significant increases in production and yield of PLG from a plant-based expression system without comprising the ability of the PLG product to be activated to a polypeptide capable of binding fibrin and having serine protease activity, including biologically active plasmin that is also aglycosylated. The activated aglycosylated plasmin is useful to treat diseases or conditions associated with a thrombus.

    Biologically safe transient protein expression in plants
    68.
    发明公开
    Biologically safe transient protein expression in plants 审中-公开
    Biologisch sichere transiente蛋白质表达bei Pflanzen

    公开(公告)号：EP2336335A2

    公开(公告)日：2011-06-22

    申请号：EP10011720.9

    申请日：2005-07-07

    申请人： Icon Genetics GmbH

    发明人： Marillonnet, Sylvestre ,  Engler, Carola ,  Mühlbauer, Stefan ,  Herz, Stefan ,  Werner, Stefan ,  Klimyuk, Victor ,  Gleba, Yuri

    IPC分类号： C12N15/82 ,  A01H5/12 ,  C12N15/74

    CPC分类号： C12N15/8203 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8257

    摘要： A process of producing a protein of interest by expressing a sequence of interest in a plant or in plant leaves, comprising:
    (a) transfecting said plant or said plant leaves with an Agrobacterium strain in the presence or in the absence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon,
    wherein said sequence encoding a replicon contains
    (i) sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and
    (ii) said sequence of interest to be expressed,

    (b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a),

    wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.

    摘要翻译： 通过在植物或植物叶中表达感兴趣的序列来产生感兴趣的蛋白质的方法，包括：（a）在存在或不存在补体因子的情况下，用农杆菌菌株转染所述植物或所述植物叶， 所述含有T-DNA的农杆菌菌株具有编码复制子的序列部分的异源DNA序列，其中所述编码复制子的序列包含（i）所述复制子的复制子功能所需的序列，所述序列衍生自植物病毒，和 ii）要表达的所述感兴趣的序列，（b）任选地将所述植物或所述植物叶中的所述目的蛋白分离入步骤（a），其中所述农杆菌菌株被提供第一遗传修饰，使得所述农杆菌菌株为 在没有所述补体因子的情况下用所述T-DNA转染生物体。