公开(公告)号：EP0311054A2

公开(公告)日：1989-04-12

申请号：EP88116500.5

申请日：1988-10-05

申请人： MERCIAN CORPORATION

发明人： Yoshimoto, Akihoro ,  Jodo Osamu Sanraku Hodogayaryo ,  Watanabe, Yoshio ,  Okamoto, Rokuro ,  Ishikura, Tomoyuki ,  Naganawa, Hiroshi ,  Sawa, Tsutomu ,  Takeuchi, Tomio

IPC分类号： C07H15/252 ,  A61K31/70

CPC分类号： C07H15/252

摘要： Disclosed are anthracycline antibiotics of a formula (I):

in which R¹ and R² are hydroxyl groups, R³ is ethyl group, R⁴ is methoxycarbonyl group and X represents daunosamine-rhodenose or daunosamine-deoxyfucose; or
R¹ and R² are hydroxyl groups, R³ is 1-hydroxyethyl group, R⁴ is hydrogen atom and X represents daunosamine-­rhodenose or daunosamine-deoxyfucose; or
R¹, R² and R⁴ are hydroxyl groups, R³ is ethyl group and X represents daunosamine-rhodenose, daunosamine-­deoxyfucose, rhodosamine-rhodenose, N-­monomethyldaunosamine-rhodenose or N-­monomethyldaunosamine-deoxyfucose; or
R¹ is methoxy group, R² is hydroxyl group, R³ is ethyl group, R⁴ is methoxycarbonyl group and X represents daunosamine-rhodenose or daunosamine-deoxyfucose; or
R¹ and R⁴ are hydroxy groups, R² is hydrogen atom, R³ is ethyl group and X represents daunosamine-deoxyfucose, as well as pharmaceutically acceptable acid addition salts thereof. The antibiotics (I) have a carcinostatic activity and are useful as a carcinostatic.

摘要翻译： 公开了式（I）的蒽环类抗生素：其中R 1和R 2为羟基的基团为CHEM，R 3为乙基，R 4为甲氧基羰基，X为道诺胺 - 罗丹诺 或柔红霉素 - 脱氧岩藻糖; 或R 1和R 2为羟基，R 3为1-羟基乙基，R 4为氢原子，X为道诺明胺 - 罗丹诺或柔红霉素脱氧岩藻糖; 或R 1，R 2和R 4为羟基，R 3为乙基，X为道诺胺 - 罗丹诺，柔红霉素 - 脱氧岩藻糖，罗丹明 - 罗丹诺，N-单甲基丹麦胺 - 罗丹诺或N-单甲基丹麦胺 -deoxyfucose; 或R 1为甲氧基，R 2为羟基，R 3为乙基，R 4为甲氧基羰基，X为道诺明胺 - 罗丹诺或丹参胺脱氧岩藻糖; 或R 1和R 4为羟基，R 2为氢原子，R 3为乙基，X为道诺胺脱氧岩藻糖，以及其药学上可接受的酸加成盐。 抗生素（I）具有制癌活性，可用作制癌剂。

公开(公告)号：EP2963104A1

公开(公告)日：2016-01-06

申请号：EP14756464.5

申请日：2014-02-28

申请人： MERCIAN CORPORATION

发明人： OKOSHI Takashi ,  TANZAWA Fumiko ,  SUZUKI Katsuhiko

IPC分类号： C12G1/02 ,  C12G3/02

CPC分类号： C12G1/02 ,  C12G1/0203 ,  C12G1/0213 ,  C12G3/02 ,  C12G2200/21

摘要： A method is disclosed for manufacturing fruit liquor in which muscat fragrance is emphasized. The method includes either or both of set of step (a) and step (d) and another set of step (b) and step (c). Step (a) is to obtain fruit juice or fruit juice containing pericarp, by crushing and squeezing the fruit part of grapes. Step (b) is to heat the fruit juice or the fruit juice containing pericarp obtained in step (a) so as to make the article temperature reach 45 to 95 °C. Step (c) is to add enzyme having the glycosidase activity at the article temperature of fruit juice in a range of 10 to 55 °C. Step (d) is to ferment the fruit juice obtained in step (b) or step (c) using a yeast having the β-glucosidase activity. The fruit liquor manufactured by this method has favorable muscat fragrance.

公开(公告)号：EP1770165B1

公开(公告)日：2011-12-21

申请号：EP05766205.8

申请日：2005-07-19

申请人： Eisai R&D Management Co., Ltd. ,  MERCIAN CORPORATION

发明人： MACHIDA, Kazuhiro ,  ARISAWA, Akira ,  TAKEDA, Susumu ,  YOSHIDA, Masashi ,  TSUCHIDA, Toshio

IPC分类号： C12N15/52 ,  C07K14/36 ,  C12N9/00 ,  C12N9/02 ,  C12P17/16 ,  C12P17/08

CPC分类号： C12P17/08 ,  C07K14/36 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/52 ,  C12P17/162

摘要： A polypeptide participating in the biosynthesis of pladienolide as a macrolide compound; a DNA coding for such a polypeptide; a variant thereof; a transformant in which part or the whole of such a DNA or variant thereof has been incorporated; and a process for producing pladienolide as a macrolide compound with the use of such a transformant. In particular, there is provided an isolated pure DNA comprising at least one region coding for a polypeptide participating in the biosynthesis of pladienolide. Further, there are provided a polypeptide coded for by this DNA and a recombinant plasmid capable of self-replication or integration replication carrying this DNA. Still further, there are provided a transformant carrying this DNA and a process for producing pladienolide, characterized in that this transformant is cultured in a culture medium and pladienolide is collected from the culture solution.

公开(公告)号：EP2357229A1

公开(公告)日：2011-08-17

申请号：EP07743689.7

申请日：2007-05-18

申请人： MERCIAN CORPORATION ,  National Institute of Advanced Industrial Science and Technology

发明人： FUJII, Yoshikazu ,  TAMURA, Tomohiro

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/04 ,  C12P7/02 ,  C12P33/06

CPC分类号： C12P7/22 ,  C12N9/0071 ,  C12P7/02

摘要： A vitamin D 3 hydroxylase is purified from Pseudonocardia autotrophica cell, and a primer is designed based on amino acid sequence obtained from hydroxylase. Subsequently, PCR is conducted using genomic DNA of Pseudonocardia autotrophica as a template to clone a gene for the vitamin D 3 hydroxylase. By conducting a conversion reaction using a microorganism in which the vitamin D 3 hydroxylase gene is expressed using a proper expression system, a hydroxide of vitamin D or the like (e.g., hydroxy vitamin D 3 ) can be produced with high efficiency.

摘要翻译： 从维生素D 3羟化酶纯化，并从基于羟化酶的氨基酸序列设计引物。 随后，使用假心内膜自养基因组DNA作为模板进行PCR，以克隆维生素D 3羟化酶的基因。 通过使用适当的表达系统来表达维生素D 3羟化酶基因的微生物进行转化反应，可以高效率地制造维生素D等的氢氧化物（如羟基维生素D 3）。

公开(公告)号：EP2341137A1

公开(公告)日：2011-07-06

申请号：EP09819153.9

申请日：2009-10-05

申请人： MERCIAN CORPORATION ,  National Institute of Advanced Industrial Science and Technology

发明人： FUJII Yoshikazu ,  FUJII Tadashi ,  ARISAWA Akira ,  TAMURA Tomohiro

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12P33/06 ,  C12N9/02

CPC分类号： C12P21/02 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/74 ,  C12P7/02 ,  C12P7/62 ,  C12P17/06 ,  C12Y114/13013

摘要： An expression vector capable of expressing a foreign gene in Pseudonocardia autotrophica; a transformant of Pseudonocardia autotrophica produced by using the expression vector; a method for producing a protein by using the transformant; a method for producing an active form of vitamin D3 from vitamin D3, which comprises highly expressing a gene encoding an enzyme involved in the synthesis of the active form of vitamin D3 in a transformant by using the expression vector or the transformant; a method for producing 25-hydroxyvitamin D2 from vitamin D2; and a method for producing pravastatin from compactin, which comprises highly expressing a compact in hydroxylase gene in a transformant by using the expression vector or the transformant.

摘要翻译： 能够表达外源基因的表达载体，其能够在自养假诺卡氏菌中表达; 通过使用表达载体产生的假心内膜自养基因的转化体; 使用该转化体生产蛋白质的方法; 从维生素D3生产活性形式的维生素D3的方法，其包括通过使用表达载体或转化体高度表达编码参与转化体中活性形式的维生素D3的活性形式的酶的基因; 从维生素D2生产25-羟基维生素D 2的方法; 以及通过使用表达载体或转化体在转化体中高度表达羟化酶基因中的紧密度的方法，从卡培生制备普伐他汀的方法。

公开(公告)号：EP2017301A1

公开(公告)日：2009-01-21

申请号：EP07742027.1

申请日：2007-04-20

申请人： MERCIAN CORPORATION

发明人： TAKATA, Ryoji ,  YAMAMOTO, Reiko ,  YANAI, Takaaki ,  KON-NO, Tomonori

IPC分类号： C08L5/00

CPC分类号： C08L5/00 ,  A23L33/10 ,  A61K31/352 ,  A61K31/715 ,  A61K36/185

摘要： An object is to establish a method of preparation of novel polysaccharides from a cassis polysaccharide (CAPS), wherein the novel polysaccharides exhibit a higher immunoregulatory effect per unit amount, have a low viscosity, and can be handled readily during the method of preparation a final product. Another object is to provide health foods and drinks having high safety and an excellent immunoregulatory effect at a low cost by utilizing a juice, processed juice or a purified product. The present invention relates to a composition of which chief ingredient is novel polysaccharides, having an average molecular weight falling within a range of 10,000 to 40,000; which is obtained by partially digestion of CAPS with enzyme, and an immunoregulatory foods and drinks utilizing the composition.

摘要翻译： 目的是建立一种从黑腹果糖多糖（CAPS）制备新型多糖的方法，其中新型多糖对每单位量表现出更高的免疫调节作用，具有低粘度，并且在制备最终的方法中可以容易地处理 产品。 另一个目的是通过使用果汁，加工的果汁或纯化产品，以低成本提供高安全性和优异的免疫调节作用的保健食品和饮料。 本发明涉及其主要成分为新型多糖的组合物，其平均分子量在10,000〜40,000的范围内; 其通过用酶部分消化CAPS获得，并且使用该组合物的免疫调节食品和饮料。

公开(公告)号：EP1200421B1

公开(公告)日：2008-08-27

申请号：EP00940911.1

申请日：2000-06-30

申请人： MERCIAN CORPORATION ,  ZAIDAN HOJIN BISEIBUTSU KAGAKU KENKYU KAI

发明人： Tsuchida, Toshio ,  Nagai, Hazuki ,  Nakashima, Takashi ,  Yoshida, Masashi ,  Konuki, Kaname ,  Kuroda, Asako ,  Isshiki, Kunio ,  Takeuchi, Tomio

IPC分类号： C07D311/76 ,  C07C69/78

CPC分类号： C07D311/76 ,  C07C67/31 ,  C07C67/343 ,  C07C67/46 ,  C07C69/92 ,  Y02P20/55 ,  C07C69/88

摘要： Provided is a process for producing an isocoumarin-3-yl-acetic acid derivative, characterized by reacting a homophthalic acid derivative represented by formula (a): (wherein Rc, R2 and R are mainly protecting groups) with a malonic acid derivating represented by formula (IV): (wherein R is an organic group including a lower alkyl group; R3 is a protecting group for a carboxyl group; and X is a halogen atom or a -OM group (wherein M is alkaline metal or alkaline earth metal)). According to the above process, various isocoumarin-3-yl-acetic acid derivatives can efficiently be provided.

公开(公告)号：EP1949889A1

公开(公告)日：2008-07-30

申请号：EP06811869.4

申请日：2006-10-17

申请人： MERCIAN CORPORATION

发明人： WATANABE, Azuma ,  IMAMURA, Kazuyuki

IPC分类号： A61K8/97 ,  A61K8/49 ,  A61K36/18 ,  A61P17/02 ,  A61P17/16 ,  A61P43/00 ,  A61L27/00

CPC分类号： A61K36/87 ,  A61K8/97 ,  A61K2800/70 ,  A61L27/60 ,  A61Q19/08

摘要： The present invention relates to a fibroblast activator comprising grape sap as an active ingredient, a method of activating a fibroblast, using grape sap, and a method of using grape sap as a fibroblast activator. The present invention further relates to a collagen synthesis promoter comprising grape sap as an active ingredient, a method of promoting collagen synthesis using grape sap, and a method of using grape sap as a collagen synthesis promoter. The present invention further relates to a skin antiaging agent comprising grape sap as an active ingredient, a method of preventing aging of skin using grape sap, and a method of using grape sap as a skin antiaging agent. The present invention provides highly safe and effective means of preventing aging.

摘要翻译： 本发明涉及一种成纤维细胞活化剂含有葡萄树液作为成分活性，激活成纤维细胞，使用葡萄树液的方法，以及使用葡萄树液作为成纤维细胞激活剂的方法。 本发明还涉及一种胶原合成促进剂含有葡萄树液作为有效成分，使用葡萄树液促销婷胶原合成的方法，以及使用葡萄树液作为胶原合成促进剂的方法。 本发明还涉及一种皮肤抗老化剂含有葡萄树液作为有效成分，使用葡萄树液防止皮肤老化的方法，以及使用葡萄树液作为皮肤抗老化剂的方法。 本发明提供了预防老化的高度安全的和有效的手段。

公开(公告)号：EP1103612B1

公开(公告)日：2008-03-26

申请号：EP99935047.3

申请日：1999-08-04

申请人： MERCIAN CORPORATION

发明人： FUJII, Tadashi ,  NARITA, Takao ,  NAKATA, Kuniho ,  AGEMATU, Hitosi ,  TSUNEKAWA, Hiroshi ,  ISSHIKI, Kunio ,  YOSHIOKA, Takeo

IPC分类号： C12N15/53 ,  C12N15/54 ,  C12N9/04 ,  C12N9/10 ,  C12N1/21 ,  C12P13/04

CPC分类号： C12N9/0004 ,  C12N9/00 ,  C12N9/1096 ,  C12P13/14

公开(公告)号：EP1770165A1

公开(公告)日：2007-04-04

申请号：EP05766205.8

申请日：2005-07-19

申请人： Eisai R&D Management Co., Ltd. ,  MERCIAN CORPORATION

发明人： MACHIDA, Kazuhiro ,  ARISAWA, Akira ,  TAKEDA, Susumu ,  YOSHIDA, Masashi ,  TSUCHIDA, Toshio

IPC分类号： C12N15/52 ,  C07K14/36 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12P17/16

CPC分类号： C12P17/08 ,  C07K14/36 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/52 ,  C12P17/162

摘要： The present invention provides polypeptides that participate in the biosynthesis of the pladienolide macrolide compounds, DNA that encodes these polypeptides and variants of this DNA, transformants that maintain all or a portion of this DNA or variant thereof, and a method of producing the pladienolide macrolide compounds using these transformants. More particularly, it provides an isolated pure DNA that contains at least one region encoding a polypeptide that participates in pladienolide biosynthesis; polypeptide encoded by this DNA; a self-replicating or integrated-replicating recombinant plasmid carrying this DNA; a transformant maintaining this DNA; and a method of producing a pladienolide, characterized by culturing this transformant on culture medium and collecting pladienolide from this culture medium.

摘要翻译： 本发明提供了参与扁桃苷内酰胺大环内酯化合物的生物合成的多肽，编码这些多肽的DNA和该DNA的变体，保持该DNA或其变体的全部或一部分的转化体，以及生产该扁桃苷内酰胺大环内酯化合物的方法 使用这些转化体。 更具体地，其提供了分离的纯DNA，其含有至少一个编码参与泛二烯内酯生物合成的多肽的区域; 由该DNA编码的多肽; 携带该DNA的自复制或整合复制重组质粒; 维持该DNA的转化体; 以及生产普拉二烯内酯的方法，其特征在于在培养基上培养该转化体并从该培养基中收集普拉地奈内酯。