Transformed bacteria homologous recombination frequency is increased

    公开(公告)号:JP4625318B2

    公开(公告)日:2011-02-02

    申请号:JP2004353026

    申请日:2004-12-06

    发明人: 理 高橋

    摘要: The purpose of the present invention is to provide a strain having a significantly increased frequency of homologous recombination, which is necessary for gene disruption of gene replacement by gene targeting of a mitosporic filamentous fungus. This invention relates to a transformant having an increased frequency of homologous recombination (gene targeting), which is a mitosporic filamentous fungus belonging to Trichocomaceae, due to suppression of a ku gene by ku gene disruption or antisense RNA method, to a method for the production of a gene-disruption stain, gene-deletion strain, gene-replacement strain, gene-insertion strain or chromosome-modification strain by means of the gene targeting method using said transformant, and to the above-mentioned ku genes such as those derived from Aspergillus sojae and Aspergillus oryzae, and their expressed products (proteins).