公开(公告)号：US5942660A

公开(公告)日：1999-08-24

申请号：US628039

申请日：1996-04-04

申请人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

发明人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

IPC分类号： C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/82 ,  C12P7/62 ,  A01H5/00 ,  C12N5/14 ,  C12N15/31 ,  C12N15/52

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12N9/00 ,  C12N9/1029 ,  C12N9/88 ,  C12P7/625

摘要： Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer end product. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

摘要翻译： 提供了通过使用重组DNA技术操作正常代谢途径来优化在植物和细菌中3-羟基丁酸酯（3HB）和3-羟基戊酸酯（3HV）的共聚物的生物合成中使用的底物水平的基因和方法。 这通过使用参与天冬氨酸家族氨基酸的生物合成的各种野生型和/或失调的酶以及涉及苏氨酸转化的野生型或去调节形式的酶，例如苏氨酸脱氨酶来实现 P（3HB-co-3HV）共聚物最终产物。 通过这些方法，产生了苏氨酸，α-酮丁酸酯，丙酸酯，丙酰基-CoA，β-酮戊酰-CoA和β-羟基戊酰-CoA的增强水平。 还提供了在植物和细菌中利用通过各种工程化代谢途径产生的丙酰辅酶A生物生产P（3HB-co-3HV）共聚物的方法。 单独或与参与天冬氨酸家族氨基酸生物合成的各种酶以及将苏氨酸转化为PHA共聚物前体的合适的β-酮硫解酶，β-酮酰基-CoA还原酶和PHA合酶的植物和细菌的引入将允许这些 生物体产生P（3HB-co-3HV）共聚物。

公开(公告)号：US5958745A

公开(公告)日：1999-09-28

申请号：US673388

申请日：1996-06-28

申请人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

发明人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/82 ,  C12P7/62 ,  A61K39/395 ,  C07K14/195

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12N9/00 ,  C12N9/1029 ,  C12N9/88 ,  C12P7/625

摘要： Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer endproduct. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

摘要翻译： 提供了通过使用重组DNA技术操作正常代谢途径来优化在植物和细菌中3-羟基丁酸酯（3HB）和3-羟基戊酸酯（3HV）的共聚物的生物合成中使用的底物水平的基因和方法。 这通过使用参与天冬氨酸家族氨基酸的生物合成的各种野生型和/或失调的酶以及涉及苏氨酸转化的野生型或去调节形式的酶，例如苏氨酸脱氨酶来实现 P（3HB-co-3HV）共聚物末端产物。 通过这些方法，产生了苏氨酸，α-酮丁酸酯，丙酸酯，丙酰基-CoA，β-酮戊酰-CoA和β-羟基戊酰-CoA的增强水平。 还提供了在植物和细菌中利用通过各种工程化代谢途径产生的丙酰辅酶A生物生产P（3HB-co-3HV）共聚物的方法。 单独或与参与天冬氨酸家族氨基酸生物合成的各种酶以及将苏氨酸转化为PHA共聚物前体的合适的β-酮硫解酶，β-酮酰基-CoA还原酶和PHA合成酶的植物和细菌的引入将允许这些 生物体产生P（3HB-co-3HV）共聚物。

公开(公告)号：US06538179B1

公开(公告)日：2003-03-25

申请号：US08399023

申请日：1995-03-06

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark

IPC分类号： C12N1582

CPC分类号： C12N9/1241 ,  C12N15/8222 ,  C12N15/8235 ,  C12N15/8245 ,  C12N15/8247

摘要： Promoters for enhanced expression of ADPglucose pyrophosphorylase in potato tubers and fruits such as tomato; methods of using them; DNA molecules, plant cells and plants containing them. A method of decreasing the oil content of seeds by expression of ADPglucose pyrophosphorylase.

摘要翻译： 用于增强马铃薯块茎和水果如番茄中ADP葡萄糖焦磷酸化酶表达的启动子; 使用它们的方法; DNA分子，植物细胞和含有它们的植物。 通过ADP葡萄糖焦磷酸化酶的表达降低种子含油量的方法。

公开(公告)号：US5648249A

公开(公告)日：1997-07-15

申请号：US406858

申请日：1995-03-20

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark ,  James Conrad Zalewski

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark ,  James Conrad Zalewski

IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N9/12 ,  C12N15/09 ,  C12N15/54 ,  C12N15/82 ,  C12N15/31 ,  C12N15/62

CPC分类号： C12N9/1241 ,  C12N15/8245

摘要： The invention relates to a method of improving the quality of potatoes stored at reduced temperatures and a method of prolonging dormancy of stored potato tubers, by increasing the level of ADPglucose pyrophosphorylase enzyme activity within the potato tuber during storage at ambient or reduced temperatures. Novel DNA molecules, plant cells, and potato plants are provided which contain the gene for the ADPglucose pyrophosphorylase enzyme.

摘要翻译： PCT No.PCT / US94 / 05275第 371日期1995年3月20日 102（e）1995年3月20日PCT PCT 1994年5月18日PCT公布。 第WO94 / 28149号公报 日期1994年12月8日本发明涉及通过在环境储存期间提高马铃薯块茎内的ADP葡萄糖焦磷酸化酶活性的水平，提高在降低温度下储存的马铃薯的质量的方法和延长储存的马铃薯块茎的休眠的方法 降低温度 提供了含有ADP葡萄糖焦磷酸化酶基因的新型DNA分子，植物细胞和马铃薯植物。