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公开(公告)号：US20090029430A1
公开(公告)日：2009-01-29
申请号：US11665065
申请日：2005-10-20
CPC分类号： C12N9/0006 , C12P7/04 , C12P7/62 , C12P13/002 , C12P41/002
摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.
摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离的3-氧代-3-苯基丙腈的活性的多肽，以产生（R）-3-羟基-3-苯基丙腈，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。
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公开(公告)号：US20070178565A1
公开(公告)日：2007-08-02
申请号：US11730549
申请日：2007-04-02
CPC分类号： C12P17/10 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供了有效形成（R）-N-苄基-3-吡咯烷醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADH或NADPH作为辅酶作用于N-苄基-3-吡咯烷酮，形成（R） - N-苄基-3-吡咯烷醇; （2）活性最佳pH：5.5〜6.0; （3）活性最佳温度：50〜55℃。 （4）通过凝胶过滤分析确定的分子量：约55,000，通过SDS聚丙烯酰胺凝胶电泳分析确定约28,000。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。
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公开(公告)号：US07220564B2
公开(公告)日：2007-05-22
申请号：US10528000
申请日：2003-09-19
CPC分类号： C12P17/10 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same.The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供了有效形成（R）-N-苄基-3-吡咯烷醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADH或NADPH作为辅酶作用于N-苄基-3-吡咯烷酮，形成（R） - N-苄基-3-吡咯烷醇; （2）活性最佳pH：5.5〜6.0; （3）活性最佳温度：50〜55℃。 （4）通过凝胶过滤分析确定的分子量：约55,000，通过SDS聚丙烯酰胺凝胶电泳分析确定约28,000。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。
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4.发明申请Optically active 2-allylcarboxylic acid derivative and process for producing the same 失效光学活性2-烯丙基羧酸衍生物及其制备方法公开(公告)号：US20060223152A1
公开(公告)日：2006-10-05
申请号：US10553394
申请日：2004-04-16
申请人： Kazumi Okuro , Susumu Amano , Noriyuki Kizaki , Teruaki Takesue , Masaru Mitsuda , Noriyuki Ito , Yoshihiko Yasohara
发明人： Kazumi Okuro , Susumu Amano , Noriyuki Kizaki , Teruaki Takesue , Masaru Mitsuda , Noriyuki Ito , Yoshihiko Yasohara
CPC分类号： C07C233/18 , C07B2200/07 , C07C51/06 , C07C67/20 , C07C233/05 , C07C271/64 , C12P7/40 , C12P41/005 , C07C57/03 , C07C69/533
摘要： The present invention provides a process for producing an optically active 2-allylcarboxylic acid derivative, which is useful as a pharmaceutical intermediate, from readily available and inexpensive starting materials by the process which can be practiced on a commercial scale in a simple and easy manner, and certain 2-allylcarboxamide derivatives, which are novel and important intermediates in that process. An N-allylcarboxamide derivative undergoes rearrangement reaction diastereoselectively in the presence of a base to give a 2-allylcarboxamide derivative, the resulting derivative is subjected to a carbamation reaction and solvolysis to give an optically active 2-allylcarboxylic acid ester, and then the ester obtained is stereoselectively hydrolyzed using an enzyme to produce 2-allylcarboxylic acid having a high optical purity. In addition, the present invention provides a 2-allylcarboxamide derivative compound which is a novel intermediate in the process of the present invention.
摘要翻译： 本发明提供了一种用于制备可用作药物中间体的光学活性2-烯丙基羧酸衍生物的方法，其由易于获得且廉价的原料通过可以简单和容易的方式在商业规模上实施的方法制备， 和某些2-烯丙基羧酰胺衍生物，它们是该方法中新颖且重要的中间体。 N-烯丙基甲酰胺衍生物在碱存在下非对映选择性地进行重排反应，得到2-烯丙基甲酰胺衍生物，所得衍生物进行氨基甲酰化反应和溶剂解，得到光学活性2-烯丙基羧酸酯，然后得到所述酯 使用酶进行立体选择性水解以产生具有高光学纯度的2-烯丙基羧酸。 此外，本发明提供了在本发明的方法中是新型中间体的2-烯丙基甲酰胺衍生物化合物。
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![Process for the preparation of optically active 2-[6-hydroxymethyl)-1,3-dioxan-4yl]acetic acid derivatives](/abs-image/US/2002/10/29/US06472544B1/abs.jpg.150x150.jpg)
5.发明授权Process for the preparation of optically active 2-[6-hydroxymethyl)-1,3-dioxan-4yl]acetic acid derivatives 有权制备光学活性2-Ä6-（羟甲基）-1,3-二恶烷-4-基乙酸衍生物的方法公开(公告)号：US06472544B1
公开(公告)日：2002-10-29
申请号：US09509998
申请日：2000-08-16
申请人： Noriyuki Kizaki , Yukio Yamada , Yoshihiko Yasohara , Akira Nishiyama , Makoto Miyazaki , Masaru Mitsuda , Takeshi Kondo , Noboru Ueyama , Kenji Inoue
发明人： Noriyuki Kizaki , Yukio Yamada , Yoshihiko Yasohara , Akira Nishiyama , Makoto Miyazaki , Masaru Mitsuda , Takeshi Kondo , Noboru Ueyama , Kenji Inoue
IPC分类号： C07D31906
CPC分类号： C07D319/06 , C07B2200/07 , C07C67/343 , C07C253/30 , C12P7/62 , C07C69/716 , C07C255/21
摘要： This invention provides a process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivatives, which are of value as intermediates of drugs, from inexpensive starting materials without using any special equipment such as that required for super-low temperature reactions. A process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivatives which comprises reacting an acetic acid derivative at a temperature of not less than −30° C. with an enolate prepared by permitting either a base or a metal having a valency of 0 to act on the derivative to produce a hydroxyoxohexanoic acid derivative, reducing this compound with the aid of a strain of microorganism to provide a halomethyldioxanylacetic acid derivative, treating this compound with an acetalizing agent in the presence of an acid catalyst to provide a halomethyldioxanylacetic acid derivative, reacted with an acyloxylating agent to provide a acyloxymethyldioxanylacetic acid derivative, and subjecting this compound to solvolysis in the presence of a base.
摘要翻译： 本发明提供了从廉价起始原料生产具有价值作为药物中间体的光学活性的2- [6-（羟甲基）-1,3-二恶烷-4-基]乙酸衍生物的方法，而不使用任何特殊设备 例如超低温反应所需的方法。一种制备光学活性的2- [6-（羟甲基）-1,3-二恶烷-4-基]乙酸衍生物的方法，其包括在不是的温度下使乙酸衍生物反应 小于-30℃，通过使碱金属或化合价为0的金属作用于衍生物以制备羟基氧代己酸衍生物而制备的烯醇化物，借助于微生物菌株还原该化合物以提供 卤甲基二恶烷基乙酸衍生物，在酸催化剂存在下用缩醛化剂处理该化合物，得到卤代甲基二恶烷基乙酸衍生物，与酰氧基化剂反应，得到酰氧基甲基二恶烷基乙酸 在碱的存在下，将该化合物施用于溶剂分解。
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6.发明申请METHOD FOR CULTIVATING ALGA AND METHOD FOR PRODUCING ALGINIC ACID-CONTAINING COMPOSITION 审中-公开用于培养ALGA的方法和用于生产含酸性组合物的方法公开(公告)号：US20140242109A1
公开(公告)日：2014-08-28
申请号：US14343909
申请日：2012-09-06
申请人： Noriyuki Kizaki , Takeshi Furuta , Taku Mouri , Naoaki Taoka
发明人： Noriyuki Kizaki , Takeshi Furuta , Taku Mouri , Naoaki Taoka
CPC分类号： C12P19/04 , A23K10/10 , A23K10/30 , A23K20/163 , A23L33/10 , A23L33/105 , A23L33/21 , A61K31/734 , A61K36/05 , B01J20/24 , B01J20/3078 , B01J2220/4843 , C02F1/68 , C12N1/12 , C12P7/44 , C12R1/89 , Y02W10/37
摘要： The objective of the present invention is to provide a method for cultivating an alga having ability to produce alginic acid industrially and effectively and a method for producing an alginic acid-containing composition industrially advantageously. The method for cultivating an alga according to the present invention is characterized in comprising the step of cultivating the alga heterotrophically, wherein the alga belongs to the genus Parachlorella and has ability to produce alginic acid.
摘要翻译： 本发明的目的是提供一种在工业上有效地培养具有生产藻酸的能力的藻类的方法和在工业上有利地生产含海藻酸的组合物的方法。 根据本发明的培养藻类的方法的特征在于包括以异养方式培养藻类的步骤，其中藻类属于Parachlorella属并具有产生海藻酸的能力。
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公开(公告)号：US20120202889A1
公开(公告)日：2012-08-09
申请号：US13389924
申请日：2010-08-11
申请人： Hiroaki Inoue , Noriyuki Kizaki , Hirokazu Nanba
发明人： Hiroaki Inoue , Noriyuki Kizaki , Hirokazu Nanba
CPC分类号： A61K31/01 , A23K20/174 , A23K20/179 , A61K31/122 , C12P23/00
摘要： The present invention relates to a method for reducing the relative ratio of 3-hydroxy-3′,4′-didehydro- β, Ψ-caroten-4-one (HDCO) to astaxanthin in a composition containing astaxanthin and HDCO by contacting the composition with an acidic medium having a pH of 3 or less and/or a basic medium having a pH of 9 or greater, and also relates to a method for producing an astaxanthin-containing composition which includes reducing the relative ratio of HDCO by the above method. By means of the method of the present invention, the relative ratio of HDCO, the biological function of which is not known, in an astaxanthin-containing composition can be easily reduced.
摘要翻译： 本发明涉及一种降低含有虾青素和HDCO的组合物中3-羟基-3'，4'-二脱氢糠醛，Ψ-胡萝卜素-4-酮（HDCO）与虾青素的相对比例的方法， pH为3以下的酸性介质和/或pH为9以上的碱性介质的组合物，还涉及含有虾青素的组合物的制造方法，其包括降低HDCO与上述相对的比例 方法。 通过本发明的方法，可以容易地降低含有虾青素的组合物中HDCO（其生物学功能不知道）的相对比例。
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公开(公告)号：US08008461B2
公开(公告)日：2011-08-30
申请号：US11665065
申请日：2005-10-20
IPC分类号： C07H21/04 , C12N9/02 , C12P21/02 , C12P13/00 , C07C255/01
CPC分类号： C12N9/0006 , C12P7/04 , C12P7/62 , C12P13/002 , C12P41/002
摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.
摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离的3-氧代-3-苯基丙腈的活性的多肽，以产生（R）-3-羟基-3-苯基丙腈，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。
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公开(公告)号：US07332312B2
公开(公告)日：2008-02-19
申请号：US10512908
申请日：2003-04-30
CPC分类号： C12N9/0006 , C12Y101/01184
摘要： The present invention provides a novel polypeptide forming (R)-2-chloro-1-(3′-chlorophenyl)ethanol, a polynucleotide coding for said polypeptide, and use of the same.The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on 2-chloro-1-(3′-chlorophenyl)ethanone with NADPH or NADH as a coenzyme, to form (R)-2-chloro-1-(31-chlorophenyl)ethanol; (2) optimum pH for activity: 5.0 to 6.0; (3) optimum temperature for activity: 40° C. to 50° C.; (4) molecular weight: about 40,000 as determined by gel filtration analysis, about 30,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供形成（R）-2-氯-1-（3'-氯苯基）乙醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADPH或NADH作为辅酶作用于2-氯-1-（3'-氯苯基）乙酮， 形成（R）-2-氯-1-（31-氯苯基）乙醇; （2）活性最佳pH：5.0〜6.0; （3）最适温度为40℃〜50℃。 （4）分子量：约40,000，通过凝胶过滤分析测定，约30,000，通过SDS聚丙烯酰胺凝胶电泳分析测定。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。
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公开(公告)号：US07169592B2
公开(公告)日：2007-01-30
申请号：US10112706
申请日：2002-04-02
申请人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Keiji Matsumoto , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
发明人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Keiji Matsumoto , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
CPC分类号： C12P13/001 , C12N9/1096
摘要： To provide a method for preparing optically active compounds, which mainly contain (R)-amino compounds, by microbial enzymes efficiently and inexpensively; a polypeptide having stereoselective transaminase activity which can be suitably used for the above preparation method; and a DNA encoding the polypeptide. A method for preparing an optically active amino compound, characterized in stereoselectively transaminating by acting a transaminase on an amino group acceptor, a ketone compound in the presence of an amino group donor, a primary amine; a DNA comprising a nucleotide sequence encoding a polypeptide having stereoselective transaminase activity; and a polypeptide having stereoselective transaminase activity obtainable from a culture of a microorganism belonging to the genus Arthrobacter.
摘要翻译： 提供通过微生物酶有效且廉价地制备主要含有（R） - 氨基化合物的光学活性化合物的方法; 具有立体选择性转氨酶活性的多肽，可适用于上述制备方法; 和编码该多肽的DNA。 一种制备光学活性氨基化合物的方法，其特征在于通过在氨基受体上起转氨酶的作用，在氨基供体存在下的酮化合物，伯胺，立体选择性转氨酶; 包含编码具有立体选择性转氨酶活性的多肽的核苷酸序列的DNA; 和具有立体选择性转氨酶活性的多肽，其可从属于节杆菌属的微生物的培养物获得。
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