公开(公告)号：US08361748B2

公开(公告)日：2013-01-29

申请号：US13528162

申请日：2012-06-20

申请人： Kenzo Yokozeki ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo Yokozeki ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/06

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

摘要翻译： 通过从L-天冬氨酸-α，二酯和L形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 - 苯丙氨酸，其使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α，α-二酯的α-酯位点的酶或酶的物质。

公开(公告)号：US20120301918A1

公开(公告)日：2012-11-29

申请号：US13528162

申请日：2012-06-20

申请人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/02

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

公开(公告)号：US20120003692A1

公开(公告)日：2012-01-05

申请号：US13198075

申请日：2011-08-04

申请人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/02

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

摘要翻译： 通过从L-天冬氨酸-α，二酯和L形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 - 苯丙氨酸，其使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α，α-二酯的α-酯位点的酶或酶的物质。

公开(公告)号：US08034584B2

公开(公告)日：2011-10-11

申请号：US12749966

申请日：2010-03-30

申请人： Kenzo Yokozeki ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo Yokozeki ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/06

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-αβ-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-αβ-diester through a peptide bond.

摘要翻译： 通过从L-天冬氨酸-α和bgr-二酯和L-苯丙氨酸形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α和bgr-二酯的α-酯位点的酶或酶的物质。

公开(公告)号：US07736876B2

公开(公告)日：2010-06-15

申请号：US12141428

申请日：2008-06-18

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US07278876B2

公开(公告)日：2007-10-09

申请号：US11645467

申请日：2006-12-26

申请人： Seiichi Hara ,  Kiminobu Tabata ,  Takeshi Kai ,  Tsutomu Fukui ,  Masaru Shinmura ,  Tadashi Inazu ,  Shigeki Takahashi

发明人： Seiichi Hara ,  Kiminobu Tabata ,  Takeshi Kai ,  Tsutomu Fukui ,  Masaru Shinmura ,  Tadashi Inazu ,  Shigeki Takahashi

IPC分类号： H01R13/85

CPC分类号： H01R13/582

摘要： A connector includes two housings coupled with each other to hold a cable therebetween. One of the housings includes a cable receiving portion for receiving the cable, a restricting portion adjacent to the cable receiving portion, a first wall portion formed outside the restricting portion and defining a space between the first wall portion and the restricting portion, and a first engaging portion inwardly protruding from the first wall portion. Another of the housings includes a cable pressing portion for pressing the cable towards the cable receiving portion and the restricting portion, and a second wall portion formed adjacent to the cable pressing portion and inserted into the space. The second wall portion has a second engaging portion extending outward from the second wall portion. The first and the second engaging potions are engaged with each other to prevent the housings from separating from each other.

公开(公告)号：US07223589B2

公开(公告)日：2007-05-29

申请号：US10879305

申请日：2004-06-30

申请人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

发明人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

IPC分类号： C12N1/20 ,  C12P1/00 ,  C07H21/04

CPC分类号： C12P19/38 ,  C12P19/40

摘要： The present invention provides a microorganism for producing a 2′-deoxyribonucleoside. In particular, the microorganism of the present invention is transformed with a gene encoding a ribonucleotide reductase and in which 2′-deoxyribonucleoside degradation activity is decreased or eliminated by disrupting a gene encoding a purine nucleoside phosphorylase on chromosomal DNA.

摘要翻译： 本发明提供了用于制备2'-脱氧核糖核苷的微生物。 特别地，本发明的微生物用编码核糖核苷酸还原酶的基因转化，通过在染色体DNA上破坏编码嘌呤核苷磷酸化酶的基因来降低或消除2'-脱氧核糖核苷降解活性。

公开(公告)号：US20060177893A1

公开(公告)日：2006-08-10

申请号：US11330076

申请日：2006-01-12

申请人： Kenzo Yokozeki ,  Isao Abe ,  Seiichi Hara

发明人： Kenzo Yokozeki ,  Isao Abe ,  Seiichi Hara

IPC分类号： C12P21/06 ,  C12N1/21 ,  C07K5/04

CPC分类号： C12P21/02

摘要： The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.

摘要翻译： 本发明提供了一种通过工业上有利且简单的途径以低成本获得的起始材料制备二肽的方法。 通过使用具有从氨基酸酯和氨基酸产生二肽的能力的微生物培养物，从培养物分离的微生物细胞或经处理的微生物细胞产物，由氨基酸酯和氨基酸产生二肽。

公开(公告)号：US20050208632A1

公开(公告)日：2005-09-22

申请号：US10879305

申请日：2004-06-30

申请人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

发明人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P19/38 ,  C12P19/40 ,  C12R1/19 ,  C12P21/04 ,  C12P19/30

CPC分类号： C12P19/38 ,  C12P19/40

摘要： A 2′-deoxyribonucleoside is produced by culturing a microorganism, which is transformed with a gene encoding a ribonucleotide reductase and in which 2′-deoxyribonucleoside degradation activity is decreased or eliminated by disrupting a gene encoding a purine nucleoside phosphorylase on chromosomal DNA, in a medium in which the microorganism can grow to produce the 2′-deoxyribonucleoside.

摘要翻译： 通过培养用编码核糖核苷酸还原酶的基因转化的微生物，通过在染色体DNA上破坏编码嘌呤核苷磷酸化酶的基因而降低或消除2'-脱氧核糖核苷降解活性的2'-脱氧核糖核苷 其中微生物能够生长以产生2'-脱氧核糖核苷的培养基。

公开(公告)号：US20050176095A1

公开(公告)日：2005-08-11

申请号：US10504988

申请日：2002-08-20

申请人： Genryou Umitsuki ,  Osamu Hatamoto ,  Seiichi Hara ,  Tsutomu Masuda ,  Motoaki Sano ,  Masayuki Machida

发明人： Genryou Umitsuki ,  Osamu Hatamoto ,  Seiichi Hara ,  Tsutomu Masuda ,  Motoaki Sano ,  Masayuki Machida

IPC分类号： C12N15/09 ,  C07K14/38 ,  C12N1/14 ,  C12N1/15 ,  C12N9/50 ,  C12N9/62 ,  C12N15/31 ,  C12P21/00 ,  C12R1/69 ,  C12P21/06 ,  C07K14/37 ,  C12N1/16 ,  C12N15/74

CPC分类号： C07K14/38

摘要： This invention provides a transcription factor that has functions of regulating the expression of a sulfur-assimilatory gene of koji mold during culture, and a gene encoding the same. This invention also relates to a protein comprising the amino acid sequence as shown in SEQ ID NO: 3, and a gene encoding the amino acid sequence as shown in SEQ ID NO: 3 or comprising the nucleotide sequence as shown in SEQ ID NO: 2.

摘要翻译： 本发明提供了具有调节培养期间曲霉的硫同化基因的表达的功能的转录因子和编码其的基因。 本发明还涉及包含SEQ ID NO：3所示的氨基酸序列的蛋白质和编码SEQ ID NO：3所示的氨基酸序列的基因，或包含SEQ ID NO：2所示的核苷酸序列 。