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    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
    2.
    发明授权
    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum 有权
    使用来自青霉菌的纤维二糖水解酶-1的超活性纤维素酶制剂

    公开(公告)号：US08283150B2

    公开(公告)日：2012-10-09

    申请号：US12247594

    申请日：2008-10-08

    申请人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    发明人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    IPC分类号： C07K1/00 ,  C07K14/00 ,  C07K17/00 ,  C12N9/42 ,  A23L1/202

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01091

    摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

    摘要翻译： 与其他Cel7家族成员酶相比，来自青霉菌（Penicillium funiculosum）的纯化纤维二糖水解酶I（糖基水解酶家族7（Cel7A））酶表现出高水平的特异性，当用来自解纤维解卷菌的纯化的EIcd内切葡聚糖酶配制并在预处理的玉米秸秆上进行测试时。 纯化的天然酶以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶的结果是真实的。 在具体实例中，与使用来自里氏木霉的纯化Cel7A的制剂相比，使用在泡盛曲霉中表达的来自青霉菌的纯化重组Cel7A的制剂的具体表现增加了超过200％。

    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
    3.
    发明授权
    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum 有权
    使用来自青霉菌的纤维二糖水解酶-1的超活性纤维素酶制剂

    公开(公告)号：US07449550B2

    公开(公告)日：2008-11-11

    申请号：US10557589

    申请日：2003-02-27

    申请人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    发明人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    IPC分类号： C07K14/00

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01091

    摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

    摘要翻译： 与其他Cel7家族成员酶相比，纯化的纤维二糖水解酶I（糖基水解酶家族7（Cel7A））来自A. cellulolyticus的纯化EIcd内切葡聚糖酶，并与预处理的玉米秸秆进行了测试，结果表明： 纯化的天然酶，以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶。在一个具体实例中，使用在A.A中表达的来自Penicillium funiculosum的纯化重组Cel7A的制剂的具体性能。 与使用来自里氏木霉的纯化Cel7A的制剂相比，泡盛曲增加超过200％。

    Single zymomonas mobilis strain for xylose and arabinose fermentation
    4.
    发明授权
    Single zymomonas mobilis strain for xylose and arabinose fermentation 失效
    用于木糖和阿拉伯糖发酵的单一运动发酵单胞菌菌株

    公开(公告)号：US5843760A

    公开(公告)日：1998-12-01

    申请号：US851767

    申请日：1997-05-06

    申请人： Min Zhang ,  Yat-Chen Chou ,  Stephen K. Picataggio ,  Mark Finkelstein

    发明人： Min Zhang ,  Yat-Chen Chou ,  Stephen K. Picataggio ,  Mark Finkelstein

    IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12P7/06 ,  C12N15/74

    CPC分类号： C12N9/10 ,  C12N15/52 ,  C12N9/90 ,  C12P7/065 ,  C12R1/01 ,  Y02E50/17 ,  Y10S435/822

    摘要： This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.

    摘要翻译： 本发明涉及通常不发酵戊糖的单一微生物，其经遗传改变以发酵戊糖，木糖和阿拉伯糖以产生乙醇，以及利用该微生物的发酵方法。 实例包括用木糖异构酶的大肠杆菌基因，木酮糖激酶，L-阿拉伯糖异构酶，L-核糖激酶，L-核酮糖-5-磷酸4-差向异构酶，转醛醇酶和转酮酶的组合转化的运动发酵单胞菌。 添加基因的表达在运动发酵单胞菌启动子的控制下。 这些新产生的微生物可用于发酵通过半纤维素和纤维素或淀粉的水解产生的葡萄糖，木糖和阿拉伯糖，以产生乙醇。

    Superactive Cellulase Formulation Using Cellobiohydrolase-1 from Penicillium Funiculosum
    9.
    发明申请
    Superactive Cellulase Formulation Using Cellobiohydrolase-1 from Penicillium Funiculosum 有权
    超临界纤维素酶配方使用来自青霉菌的纤维二糖水解酶-1

    公开(公告)号：US20090081762A1

    公开(公告)日：2009-03-26

    申请号：US12247594

    申请日：2008-10-08

    申请人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    发明人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    IPC分类号： C12N9/42

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01091

    摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

    摘要翻译： 与其他Cel7家族成员酶相比，来自青霉菌（Penicillium funiculosum）的纯化纤维二糖水解酶I（糖基水解酶家族7（Cel7A））酶表现出高水平的特异性，当用来自解纤维解卷菌的纯化的EIcd内切葡聚糖酶配制并在预处理的玉米秸秆上进行测试时。 纯化的天然酶以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶的结果是真实的。 在具体实例中，与使用来自里氏木霉的纯化Cel7A的制剂相比，使用在泡盛曲霉中表达的来自青霉菌的纯化重组Cel7A的制剂的具体表现增加了超过200％。

    Stable zymomonas mobilis xylose and arabinose fermenting strains
    10.
    发明授权
    Stable zymomonas mobilis xylose and arabinose fermenting strains 失效
    稳定的运动发酵单胞菌木糖和阿拉伯糖发酵菌株

    公开(公告)号：US07354755B2

    公开(公告)日：2008-04-08

    申请号：US09565233

    申请日：2000-05-01

    申请人： Min Zhang ,  Yat-Chen Chou

    发明人： Min Zhang ,  Yat-Chen Chou

    IPC分类号： C12N1/21 ,  C12N15/00 ,  C12N15/11

    CPC分类号： C12N9/1205 ,  C12N9/1022 ,  C12N9/92 ,  C12N15/74 ,  C12N15/90 ,  C12N15/902 ,  C12P7/06 ,  C12P7/065 ,  Y02E50/17

    摘要： The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transformed, is also provided.The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.

    摘要翻译： 本发明简要地包括用于将外来基因稳定插入到细菌基因组中的转座子，其包含至少一个操纵子，所述操纵子具有编码选自由二甲酰基A， 细菌中的结构基因，一对倒置插入序列，插入序列内部的操纵子和位于插入序列外部的转座酶基因。 一种用于将外来基因转化到细菌基因组中的质粒穿梭载体，其包含至少一个操纵子，所述操纵子具有编码选自下组的结构基因：xylAxylB，araBAD和tal / tkt，所述启动子用于在 并且还提供了与待转化的细菌基因组中的基因具有同源性的至少两个DNA片段。 根据本发明，转座子和穿梭载体可用于构建显着不同的运动发酵单胞菌菌株，其可用于使用传统发酵技术将纤维素衍生的戊糖转化为燃料和化学品，因为它们对于在 非选择介质。